JPH01172341A - Preventive and remedy for infectious disease of clostridium perfringens of domestic animal and domestic fowl - Google Patents

Abstract

PURPOSE:To obtain the title preventive and remedy having no problems of occurrence of resistant bacteria and side effects and no fear of influence of remaining agent on human body, comprising a crude drug such as root and rhizome of Glycyrrhiza glabra Linne var. glandulifera Regel et Herder, leaves of Artemlsia princeps Pampanini or rhizome of Coptis japonica Makino as an active ingredient. CONSTITUTION:The title preventive and remedy comprising a crude drug selected from the group consisting of root and rhizome of Glycyrrhiza glabra Linne var. glandulifera Regel et Herder, leaves of Artemlsia princeps Pampanini, rhizome of Coptis japonica Makino, root of Scutellarla baicalensis Georgi, bark of Phellodendron amurense Ruprecht, stems and leaves of Geranium nepalense Sweet, bark of Magnolia obovata Thumberg, root of Salvia milltiorrhiza Bunge, rhizome of Rheum palmatum Linne, rhizome of Anemanhena asphodeloides Bunge, bud of Eugenia caryophyllata Thumb., fruit of Ligustrum japonicum Thunberg, 3-5mm cut pieces of ground part of Nepeta japonica Maximovicz, dried bark of Cinna momum Cassla Blume, root of Scrophularia Oldhami Oliver, ripe seed of Cassia obtusifolla Linne, leaves of Eribotrya japonica bindley, root and rhizome of Seselilibanotic Koch var. daucifolla DC., fruit ear of Humulus Lupulus Linne, Leaves of Perilla frutescens Britton var. acuta Kudo, bark of Myrica rubra Siebold et Zuccarini, fruit of Forsythia suspensa Vahl, Aloe arborescens Mill.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a prophylactic and therapeutic agent for Clostridium perfringens infections in livestock and poultry.

More specifically, the present invention provides chicken, pig,
This invention relates to a prophylactic and therapeutic agent for Clostridium perfringens infections in cattle and sheep.

The present invention also relates to a method for preventing and treating the same infectious disease, which comprises administering crude drugs.

[Prior Art] Clostridium perfringens infection of livestock and poultry is caused by ClosLridium perl'r1ng.
It is a disease caused by infection with E. ens, and the typical symptom is necrotic enteritis, and it can also cause enterotoxemia and malignant edema. Both morbidity and mortality rates are high (and the economic damage it causes to the poultry, broiler, pig, and dairy industries is significant. Therefore, among the many diseases of livestock and poultry, it is the most important disease for which it is urgently necessary to establish control measures. It is one of the.

Scarring enteritis is a disease of piglets, chicks, etc. Clinically, diarrhea is observed, and it is characterized by bacterial invasion of the intestinal mucosa and necrosis of the small intestine.

Enterotoxemia is a disease in which Clostridium verfringens proliferates in the small intestine of animals, and the toxins it produces cause necrotic and hemorrhagic lesions, and acute death due to toxemia.

Malignant dropsy is a disease that occurs when bacteria invade the wound surface caused by an accidental or surgical operation, and the bacteria germinate, multiply, and release toxins, causing toxemia and bacteremia, which can lead to death. .

Thus, although Clostridium verfringens infection is a serious infectious disease, preventive and therapeutic methods have not yet been established.

Proper breeding management has been used as a prevention method, but in reality prevention is extremely difficult.

Furthermore, in order to prevent and treat chickenpox, methods have been used to administer antibiotics and other antibacterial agents together with feed. However, in recent years, there has been a growing trend that it is not appropriate to administer antibiotics to animals due to problems such as the emergence of resistant bacteria and the persistence of antibiotics in meat. Therefore, it is desired to develop methods and agents for preventing and treating chickenpox that do not use antibiotics or the like.

[Means for Solving the Problems] As a result of research to solve the above problems, the present inventors discovered that certain herbal medicines have the effect of suppressing this disease, and completed the present invention. Ta.

That is, an object of the present invention is to provide a preventive and therapeutic agent for Clostridium verfringens infection in livestock and poultry, and a preventive and therapeutic method according to the same agency.

The present invention covers licorice, mugwort, oren, scutellariae, scutellariae, gennoshoko, sardines, tanjin, chimo,
Rhubarb, clove, jotei, keigai, keihi, genjin, ketsumeishi, biwayo, boufuu, hop, soyo, youbaihi, forsythia, aloe, gyutan,
One or more species selected from the group consisting of Ireisen, Ubai, Enmeisou, Shazensou, Shin'ui, Inchinko, Jinkou, Senkyu, Kouhon, Gobaishi, Cornus elegans, Shikon, Kooren, Peony, Golden oxen, Thyme, White nandina, Chiyu, and Ephedra. The present invention provides a preventive and therapeutic agent for Clostridium verfringens infection in livestock and poultry, which contains the herbal medicine.

The present invention also relates to crude drugs in the form of bulk powders, organic solvents, mixtures of organic solvents and water, or crude drug extracts obtained by extraction with water;
Alternatively, the present invention provides a preventive and therapeutic agent for the same disease in livestock and poultry containing an active ingredient of a crude drug.

Furthermore, the present invention provides licorice, mugwort, oren, scutellariae, scutellariae, gennoshoko, staghorn, tanjin,
Chimo, rhubarb, clove, jotei, kegai, keihi, genjin, ketsumeishi, biwayo, boufuu, hop, soyou, youbaihi, forsythia, aloe, gyutan, ireisen, ubai, japonica, shazenso, shinii, inchinko, jinkou, senkyu, kouhon, Gobaishi, Cornelius, Shikon, Koouren,
To provide a method for preventing and treating the same disease in livestock and poultry, which comprises administering an effective amount of one or more crude drugs selected from the group consisting of peonies, goldenrod, thyme, whitenanthene, chiyu, and ephedra. It is.

The present invention also provides treatment for the same disease in mammals by administering crude drugs in the form of bulk powders, organic solvents, mixtures of organic solvents and water, or crude drug extracts obtained by extraction with water, or in the form of active ingredients of crude drugs. It provides methods of prevention and treatment.

The present invention also provides a method for preventing and treating the same disease in livestock and poultry, which comprises administering the herbal medicine in feed.

Daylily (Glye) 'rrh
lzaglabra Linne' var, gla
ndulif'era Regel et Harder
) roots and rhizomes as they are or with the cork skin removed. This product contains ingredients such as glycyrrhizin, liquiritin, glucose, and asparagine, and is used as a relaxation, antispasmodic, and sputum medicine for humans.

Mugwort (also known as Artemisia prl)
ncepsPampanin+) or sagebrush (A
, Ilontana Pampanini) leaves. This product has cineole (50%) as an ingredient, as well as
It contains essential oils and alkaloids, including α-thujone and sesquiterpenes, and is used as an astringent and hemostatic agent for uterine bleeding, etc., and also has tonic and blood supplementing effects.

Ouren (Yellow Ren) is ouren (Coptisjapo).
It is made by burning off the fine roots from the rhizome of Nica Maklno. This product contains ingredients such as berberine and palmatine, and is used as a bitter and intestinal medicine for humans.

Scutel Ia (lessor) is Scutel Ia.
rlabalcalensls Georgj) from which the cork skin has been removed. This product contains orgonin and picalin as ingredients, and is used as an anti-inflammatory and antipyretic drug for humans.

Yellowfin tuna (Pheel rodend)
ronamurense Ruprecht) or other cultivated plants, with the cork layer removed. This product contains berberine, palmatine, etc. as ingredients, and is used as a stomachic, intestinal regulation, astringent, and anti-inflammatory drug for humans.

Gen no Shoko is Gen no Shoko (Geranlum).
nepalense Swcct) in summer. This substance contains tannins, gallic acid, succinic acid, etc., and is used as an astringent for humans to treat diarrhea and abdominal pain.

Kouboku is Magnollaob.
It is the bark of the tree and branches of Thunberg ovata. This product contains essential oils whose main components are matyrol and magnolol, as well as magtucoumarin, and is used as an astringent diuretic and sputum medicine for thoracic and abdominal distension, abdominal pain, and wheezing and coughing in humans.

Dangjin (Danjin) is Dangjin (Salvlamiltl).
orrhiza Bunge). This product contains tandinone I, ■, and cryptotandinone as ingredients, and is used for human tonicity, menstruation, and as a feminine aid for menstrual disorders, uterine bleeding, abdominal pain, and various healing.

Chimo (Chimo) is Anemanhenaas
phodclojdcs Bungc).

This product contains saponin, steroid saponin,
Contains tannin, etc., and is used as an anti-inflammatory, antipyretic, hot bath, diuretic, and analgesic for humans.

The rhubarb (large seedling) is a rhubarb (Rheu palla).
tLIll Linne' or R, officlna
le Balllon) and related plants, with most of the skin removed and the rhizomes vertically divided. This product contains anthraquinone derivatives such as chrysophanol, emodin, and emodin monomethyl ether, and is used to treat chronic gastrointestinal diseases, indigestion, and constipation as a bitter, laxative, and anti-inflammatory drug for humans. .

Clove is clove (ELIgenlaca).
This is a bud of ryophylla La Thunb, ) just before flowering. This product contains eugenol,
Contains acetyl eugenol, promotes human digestive function,
It is also used as an anthelmintic, as a spice, and in cosmetics.

Jyotei (female chastity) is mouse mochi (L1gustruw)
japonicu* Thunberg). This product contains oleanolic acid, glucose, etc. as ingredients, and is used as a tonic for humans.

Keigai (荊芥) is Keigai (Nepetajapo).
It is a section of the above-ground part of the flowering stage of Nica Maxia+owicz, usually 3 to 5 m+s in length. This product contains d-menthone, d-limonene, etc. as ingredients, and is used as a sweat-fighting, fever-reducing, and antidote for humans.

Keihi (Cinnamon) is Keiji s (C1nna somu
mCassfa Blume) with the dried skin and cinnamon bark removed and the cork layer removed to some extent. This product contains cinnamaldehyde, cinnamyl acetate, etc. as ingredients, and is used for human sweating, fever reduction, analgesia, stomach health, intestinal regulation, and as an anthelmintic.

Genjin (Genjin) is Scrophularia 01dhaIli 01
iver) root. It is used in humans as an anti-inflammatory, antipyretic, analgesic, and as a drug for sexually transmitted diseases.

Ketsumeishi (Casslao) is Ebisugusa (Casslao).
It is a mature seed of btuslrolla Linne').

This product contains ingredients such as emodin and obssifoliin, and is commonly called habu tea and is used as a beverage substitute for tea.

Loquat (Er1obotryaj)
aponlca Llndley) from which the hairs of the leaves have been removed as much as possible. This substance is glucose, sucrose,
It contains fructose, maltose, etc. as ingredients, and was used as a refreshing and stomachic medicine for humans and as a preventative against hot weather.

Boufuu (windbreak) is Ibukiboufuu (Seselil)
lbanotls Koch var, daucif
olla DC,) is dried along with the rhizome. This product contains essential oils and is used as a sweating, antipyretic, and analgesic for humans when colds are accompanied by headaches, chills, and joint pain.

Hops are hops (Humulus Lupulus)
Linne') mature fructose. This product contains tannins, tumurone, rubron, etc. as ingredients, and is necessary for imparting bitterness and aroma in beer brewing.

Perilla f'rut
escensBrltton var, acuta
Kudo) leaves. This thing has an ingredient of 0.5~
Contains 1% essential oil, 55% of which is perillaldehyde.
Also includes limonene, α-pinene, etc. It is used for excitatory sweating, antipyretic, antitussive, analgesic, sedative, diuretic, stomachic, etc. in humans.

Youbaihi (fried plum skin) is a Japanese bayberry (Myricaru).
bra 5iebold at Zuccarinl)
It is the bark of the tree. This product contains tannins, myricetin, myricitrin, etc., and is used as an astringent for diarrhea in humans, and is also effective against bruises.

Forsythi
asuspcnsa Vahl) or the fruit of Forsythia japonica (P. korcanuIIOhvi). This product contains oleanolic acid etc. as a component,
It is used as a detoxifying, purulent, anti-inflammatory, and diuretic in humans.

Aloe is Aloe arborescens.
Mill.

var, naLensis Bergel,) is boiled down. This product contains barbaroin, aloe emodin, etc. as ingredients, and has been used as a bitter stomachic or laxative for humans in the Middle East and Europe since ancient times.

Gyutan (cow intestine) is a bovine intestine.
mesLicus Gselln) is hung to dry in the shade, or the bile duct is incised and the bile is sealed and stored in a container. As an ingredient, this
It contains sodium cholate, mucoprotein, fatty acids, etc., and has been used for a long time to treat jaundice, constipation, diabetes, hemorrhoids, etc. in humans.

Ireisen is Terrasen (CIeIlat1)
sMax1a+ovicziana Ranchet
et 5avatler var.

f'or1da Thunbcrg) or the roots of related plants.

It contains saponin as a component and is used as a diuretic and analgesic in humans for gout, rheumatism and neuralgia.

Prunus Muse 5
The fruit is peeled off from the unripe fruit of C. ieboldet Zuccarjni and buried in soot to dry.

The ingredients of this product include succinic acid, citric acid, malic acid, tartaric acid, etc., and it is used as a refreshing astringent, a hot bath, a worm exterminator, and an antipyretic for humans.

Enmeiso (Enmeiso) is called Hikiokoshi (Isodon).
japonica l1ara) or black-and-white tree (lsodon trichocarpus K)
Udo) contains plectrantin and enmain as ingredients, and is used as a bitter medicine for human stomach health to treat indigestion, anorexia, abdominal pain, etc.

Plantage asia LIca Linne'
) is the whole plant in flowering stage. This product contains ingredients such as aucvin and plantaginine, and is used as an anti-inflammatory and diuretic to treat eye diseases, cystitis, etc. in humans.

Shinyi is Magnolla Kob.
usA, P, De Candolle). This product contains ingredients such as citral, eugenol, and cineole, and is used as an aromatic medicine for headaches, poison healing, nasal disorders, etc.

Inchinkou (d i , W') is known as Japanese mugwort (d i , W').
ArteIIisia capillaris Thu
nberg).

This product contains terpene compounds such as kabiren, β-piben, kabiron, and kabirin, as well as isocoumarin capillarin, and is effective as an anti-inflammatory, antipyretic, and diuretic for humans to treat feverish yellow scars.

Jinko (Agarwood) is called Jinko (Aqullarlaa).
gallocha Roxb,) containing resin. This product contains benzylacetone, p-
Contains methoxybenzylacetone, hydrocinnamic acid, etc.
As a fragrant wood, it is considered to be the main ingredient in ``kodo.'' Also, for medicinal purposes, it is expected to have a kind of sedative effect and fatigue recovery effect.
Used for asthma, vomiting, abdominal pain, etc.

Cnldlumo
f'f1c1nale Maklno) roots are removed and the rhizomes are usually boiled and dried. This product contains cnidium lactone, cnidic acid, etc. as ingredients,
It is used as a human blood purifier, blood supplement, tonic, and sedative to treat anemia, sensitivity to cold, menstrual irregularities, etc.

Kouhon (Maki) is Kasamochi (Nothosmyrn).
It is the rhizome of M.iumjaponiccum Miqucl). This product contains essential oils whose main components are notosmyrnol and dimethoxyallylbenzene, and is used as an antispasmodic and analgesic for humans.

Gobaishi (Gobaishi) is the aphid (Nurudenomimifushia aphid)
Melaphls chinensls J, Be1
The sting of Rhus java
It is a gall produced in the leaves of Nica Linne' and contains tannin, gallic acid, fat, resin, etc. as components. It is used as a raw material for the production of five-fold tincture, tannic acid, gallic acid, and pyrogallol.

The cornelian cornelian (Mountain AIA) is the cornelian cornelian (Cornus
off'1c1na11s 5iebold et Z
Seeds are extracted from the mature fruits of P. ucearini). This product contains crystalline organic acids, gallic acid, malic acid, tartaric acid, etc. as ingredients, and is nourishing and tonic for humans.
It is used as an astringent.

Shikon (purple root) is purple root (Lithospermu).
＋＊offlcinalc Linne' var,
erythrorhizonMaxiIlowlcz)
It is the root of This product contains the purple pigment acetylshikonin, etc., and is used as an antipyretic and antidote for humans.

Plcrorrhiza Kurooa Royle is Plcrorrhiza Kurooa Royle.
ex Bench, ). This product contains ingredients such as kutokin, D-mannitol, and vanillic acid, and is used as a bitter stomachic and laxative for humans.

Peonies (Xuyaku)
lblf'1ora Pa1las var, tri
chocarpa Bunge). It contains benzoic acid, resin, etc. as ingredients, and is used as an astringent, relaxation, analgesic, and antispasmodic for humans.

Kinouushi (Kanaoushi) is called Naniwaibara (RosaLa).
evigata Michaelux). This product contains ingredients such as citric acid, malic acid, tannins, saponins, and vitamin C, and is used to stop diarrhea and multi-bed syndrome in humans.

Thymus is Thymus vu1g.
arlsLinne') at the flowering stage. This product contains thymol, valathymol, pinene, etc. as ingredients, and its decoction or extract is used as an antitussive for humans. It is also used in food as a flavoring agent that also serves as a preservative.

White Nanten (White Nanten) is Nandlnadowes
tlca Thunb, var, Ieucocar
It is the dried fruit of pa Makjno. This product contains components such as domesticustin and protobin (alkaloids), and is used as an antitussive for human asthma, whooping cough, etc.

Chiyu (earth elm) is Sangu1 sorba (Sangu1 sorba).
orf'1cina11s L,) was harvested and dried. This product contains tannin (1
7%), contains saponin, and is used as a hemostatic astringent for humans.
It is used for hematemesis, hemoptysis, and menstrual bleeding.

Ephedra (Ephedra 5lni)
This is the above ground stem of caStap('). This product contains components such as g-ephedrine and d-pseudoephedrine, and is used as an antitussive, diaphoretic, and diuretic for humans.

As mentioned above, the crude drugs used in the present invention include licorice, mugwort, oren, scutellariae, scutellariae, and
Kouboku, tanjin, chimo, rhubarb, clove, jijitei, keigai, keihi, genjin, ketsumeishi, biwayo, boufuu, hop, soyou, youbaihi, forsythia, aloe, gyutan, ireisen, ubai, japonica, shazenso, shinii, chinko, jinkou, Senkyu, Kouhon, Gobaishi, Cornus,
Citrus elegans, peony, thyme, thyme, cilantro, citrus, and ephedra have traditionally been used alone or in combination for the prevention and treatment of human diseases. No attempt has been made to use these for the prevention and treatment of animal diseases, particularly for the prevention and treatment of infectious diseases in animals.

The present inventors have conducted various studies focusing on the medicinal effects of the above-mentioned herbal medicines, and have found that Clostridium perfringens infection, that is, livestock and poultry
One or two of the above-mentioned herbal medicines for the prevention and treatment of diseases caused by infection with C. rfringens! They have discovered that administering the above in combination is extremely effective, and have completed the present invention.

That is, as described below, the above-mentioned specific types of herbal medicines and their extracts are effective against bacterial C in vitro and in vivo.
1 ostrldiun + perfrlngens 0 and this Clostridlu II perf
As a result of the inhibitory effect on C. ringens, when the above-mentioned herbal medicines were administered to pigs, Clostrldium per
It was possible to prevent and cure clostridial infections, which are diseases of livestock and poultry caused by P. f'ringens.

The effectiveness of the above-mentioned specific herbal medicines and their extracts against diseases specific to livestock and poultry was unexpected and was revealed for the first time by the present inventors.

These herbal medicines can be directly administered in the form of raw powder, extracts, or active ingredients of herbal medicines, or they can be added to feed, or they can be prepared in solutions, dispersions, semi-solids, powders or granules, or molded. It is used in formulations such as preparations, infusion preparations, and injection preparations.

Extracts can be obtained by solvent extraction, for example using water, an organic solvent, or a mixture of water and an organic solvent, and then left as is, or
It can be used concentrated, diluted, or with the solvent removed.

Examples of organic solvents include methanol, ethanol, n
-Propertool, n-butanol, acetone, ethyl acetate, ether, methylene chloride, chloroform, benzene, carbon tetrachloride, petroleum ether, etc. are used, and methanol and ethanol are particularly preferred. These organic solvents can be used alone or as a mixture of two or more.

This solvent extraction can be carried out by cold soaking or digesting the herbal medicine in these solvents. 15-25℃ for cold soaking
Digestion can be carried out at a temperature range of 35-45°C. The extraction time varies depending on the extraction temperature, but is generally about 5 days or until the soluble components are sufficiently dissolved.

The active ingredients of herbal medicines can be obtained by, for example, shaking the above-mentioned extracted extract using various solvents, collecting the fraction that transfers to the solvent, and distilling off the solvent, which can be used as the effective fraction, that is, the active ingredient. can.

Solvents used in this case include nonpolar solvents such as lower aliphatic ethers (diethyl ether, ethyl methyl ether, etc.); lower halogen alkanes (chloroform, ethyl methyl ether, etc.);
Examples include dichloroethane, dibromoethane, etc.); benzenes (benzene, toluene, xylene, etc.); and other petroleum solvents (petroleum ether, petroleum benzene, ligroin, etc.).

In addition, the remaining aqueous solution from which this effective fraction was extracted is extracted by shaking using water and a kind of organic solvent saturated with water, washed with water, and transferred to the organic solvent layer that is the residue. A product obtained by distilling off both solvents can also be used as an effective fraction, that is, as an active ingredient. This H-effective fraction has different physicochemical properties and different detected components from the above-mentioned effective fraction.

The dose is 0.0011 in terms of the amount of raw drug ffi.
It ranges from Ig/kg body weight/day to 100 g/kg body weight/day.

This amount differs depending on whether the drug is used as a prophylactic agent or a therapeutic agent, and the dose is generally higher in the latter case.

The administration method can be oral or parenteral administration, and examples of parenteral administration include intramuscular administration, intraperitoneal administration, transdermal administration, nasal administration, intravenous administration, and the like.

[Effects of the Invention] As detailed above, the present invention provides an effective prophylactic and therapeutic agent and an effective prophylactic and therapeutic method for Clostridium verfringens infection.

Since the prophylactic and therapeutic agent of the present invention uses herbal medicines or extracts obtained from them as herbal medicines, there is no problem of the development of resistant bacteria or side effects associated with antibiotics, etc., and it can be administered with confidence.

Furthermore, even if the animal is a domestic animal or an animal that produces eggs and milk, there is no need to worry about the effects of drug residue on the human body.

Furthermore, the disease can be prevented and treated at a lower cost than when using antibiotics or the like.

The present invention will be explained in more detail below using test examples and examples.

Test Example I Clostridium per “ringens”
The susceptibility to various herbal medicines (of bird origin and of pig origin) was investigated.

Various crude drugs were added to a 50% aqueous ethanol solution in an amount seven times the amount of the crude drugs, and extracted for 16 hours to obtain alcoholic extracts.

The extract was infiltrated into a disk of diameter 8III11 by 25 μm and dried. CIostrid this disk
1ua+ perf'ringens (of bird origin and of pig origin) were placed on a 6w blood agar plate and incubated at 37° C. for 18 hours, after which the diameter of the inhibition zone was measured.

Next, the test results are shown in Table 1.

(Margin below) Table 1 Licorice 18 using alcohol extract
19 Yomogi In
14 OWSHIN 22
25 Ougon 17
23 over 17
18 Gennosyouko 1317 Koubori E3
12 Tan Jin 17
16 chimo 19
22 die o tt
12 choji
2o 21joti
10 11 K I G I - i.

Kei Hi 16
15 Genjin-9 Table
1 (Continued) Ketsumeishi caused by alcohol extract - 9 View 13
10 bow fuu -9 ho
15 22 so
Yo 1o
15 youbaihi 2o23 forengiyo 1519 aroe 9
10 Giyuutan 9 20 Ireisen 99 U Bai II
IQ Enmeiso 17 17 Shazenso 9 10 Shin I 9
10 Inch Kou 1211 Table 1 (Continued) N/Ko by alcohol extract -
9 senkiyu 99 kouhon -9 go by shi 20 23 sanshiyuyu
1213 Sicon 10
11 Kooren 12 11 Peony 13 13 Kingfisher 14 12 Time -
11 Shironanthene 99 Chu 16 1
7 ma ou 14 15
(Left below) Example 1 Basic feed corn 450 parts by weight Milo
260 Soybean meal
190 Fish powder 50
/I yellow grease 30 Calcium carbonate 7 // Tertiary calcium phosphate 6 Salt
2 Premix 5
// 0.5% by weight of Oren powder was added to the basic feed to prepare a test feed.

760 healthy chickens, 28 days old, were fed the test feed ad libitum. The number of chickens that died due to field infection with Clostridium verfringens during 35 days from the start of the test was observed. In the control group, basal feed was given instead of the test feed.

As a result, the number of chickens that died due to field infection with Clostridium verfringens was 2 in the test plot of the present invention, whereas 58 chickens died in the control plot.

Examples 2 to 42 The same procedure as in Example 1 was conducted except that 0.6% of the crude drugs shown in Table 2 below was added instead of Oren.

Next, the test results are shown in Table 2.

Table 2 Examples Crude drug name Mortality (feather) 2 Kanzou 43
Mugwort 64 Ou Gon 45 O
Ubari 36 Genno Shoko 5 7 Koubori 58
Tang Jin 39
' Chimo 2IO Daiou 511 Choji 2 Table 2 (Continued) Examples Herbal medicine name Mortality number (feathers) 12 Jo Tei
513 Kay Guy 3
14 Kei Hi
415 Gen Jin
516 Ketsumeishi 8 17 Biwa Yo 81
8 Bow Fu 819
Hop 420
So You 521 You Baihi 2 22 Forengiyo 5 23 7 Ro 2 624
Gyutan 6 25 Ireisen 8 26 U Bai 52
7 Enmeisou 3 2B Shazenso 6 Table 2 (Continued) Example 29 Shin I
630 Jinko 4 31 Jinko 8
32 Senkyu 6 33 Kouhon 6
34 go by shi 235
Cornelius 4 36 Sikon
537 Koouren 4 38 Peony 4 39 Golden Ox 3 40 Time 8
41 Shironanthene 6 42 Chu 343
Maou 4 comparison
58 Example 44 Licorice, mugwort, oren, scutellariae, scutellariae, gennoshoko, staghorn, tanjin, chimo, rhubarb,
Clove, Jotei, Keigai, Keihi, Genjin, Ketsumeishi, Biwayo, Bofuu, Hop, Soyou, Youbaihi, Forsythia, Aloe, Gyutan, Ireisen, Ubai, Enmeiso, Shazenso, Shinii, Inchinko, Jinko, Senkyu, Kouhon, Gobaishi, Cornus, Take 1 kg of each of Sikon japonica, Oriental japonica, Peony, Ephedra, Thyme, White nanthene, Chiyu, and Ephedra, and add each to 1047 ml of water at 104°C.
Extracted for 1 hour. Each water extract was diluted 1000 times to obtain an extract.

485 healthy chickens, 28 days old, were used, and the extract was fed in place of water. The same basal feed as that used in Example 1 was used as the feed. In the control group, water was given instead of the extract. The birds were raised in this way for 35 LI from the start of the test, and the number of dead birds due to Clostridium perfringens field infection was observed at that time. As a result, the number of deaths due to Clostridium perfringens infection was lower in all test plots than in the control plot.

Example 45 Basic feed corn 450 parts by weight Milo
310 Soybean meal
146 kasu
30” fish meal
15/l meat bone powder
10 ll yellow grease 1
8. Calcium carbonate 5. Calcium phosphate 8. Food
Salt 3〃Premix
5 1/ 0.5% by weight of Oren powder was added to the basic feed to prepare a test feed. Fifty healthy pigs, 30 days old, were fed ad libitum with this test sample and raised for 60 days.

In the control group, basal feed was given instead of the test feed.

and the number of pigs that died due to Clostridium verfringens field infection during the test period and the number of Clostridium
The total number of days of diarrhea due to field infection with C. burfringens was observed. As a result, the number of pigs that died due to field infection with Clostridium verfringens was 0 in the test area of the present invention, but 7 in the control area. Additionally, the total number of pigs with diarrhea during the test period was 5/30 in the test area.
00 cows, while in the control area I11 cows/300
There were 0 head.

Examples 46 to 87 Example 4 except that in Example 45, 0.6% of the crude drug shown in Clothing 3 below was added to the basic feed instead of Oren powder.
The same procedure as in step 5 was carried out.

Next, the test results are shown in Table 3.

(Margins below) Table 3 Clostridium bar 7 Example Herbal drug name Number of deaths Total number of pigs with diarrhea due to S. ringens infection x number of days 46 Kan 190,150 6/3000 47
Yomogi 0150 8 /
300048 Scutellariae 0150 5 /3000
49 Oubaku0150 6 /300050
Genno Shoko 0150 6 /300
051 Kouboku0150 7 /300052
Danjin 0150 6 /300053 Chi
Mo 0150 5 /3000
54 Rhubarb 0150 7 /300055
Choji 0150 5 /300056 Jotei 0150 7 /300057 Keigai 0150
8 /300058 Kei Hi 0
150 6 /300059 Genjin 0150 g /300060 Ketsumeishi 0
150 8 /300G6■ Biwayo 0150
7 /30ro 062 Boufuu 0150 8
/s00゜Table 3 (Continued) Clostridium bar 7 Example Herbal drug name Number of dead pigs Total number of pigs with diarrhea due to S. ringens infection x number of days 63 Ho Tubu 0150 pigs 5 pigs/
3000 heads 64 so yo 0150
7 /300085 Youbaihi 01
50 5 /300066 Forsythia 0150
6 /300067 aroe
0150 8 /300068 Gyutan0150 5 /300069 Ireisen0150 8 /30QO70 Uba
I 0150 6 /300071
Enmeiso 0150 7 /30007
2 Shazensou 0150 6 /300
073 Shin Yi 0150
8 /300074 Inchinko 015
0 7 /300075 Jinko 0150
B/300076 Senkyu 0150
8 /300077 Kouhon0150 8 /
300078 Gobaishi0150 5 /3000
79 Cornelius 0150 7/3000 table
3 (Continued) Clostridium barf example Herbal drug name Number of dead pigs Total number of pigs with diarrhea due to S. ringens infection x number of days 80 Shikon 0150 pigs 7
/1000 heads 81 Koouren 0150
7 /300082 Peony 0
150 7 /300083 Kingfish 0150 6 /300084
Time 0150 7 /300
085 Shironanthene 0150 8
/30008G Chuyu 0150
6 /300087 Ephedra 0
150 6 /3000 control 715
0 ill /3000 (blank space below) Example 88 Licorice, mugwort, oren, scutellariae, scutellariae, gennoshoko, scutellariae, tanjin, chimo, rhubarb,
Clove, Jotei, Keigai, Keihi, Genjin, Ketsumeishi, Biwayo, Boufuu, Hop, Soyou, Youbaihi, Forsythia, Aloe, Gyutan, Ireisen, Ubai, Enmeiso, Shazenso, Shinii, Inchinko, Jinko, Senkyu, Kouhon, Gobaishi, Cornus, Take 1 kg of each of Shikon, Oriental laurel, Peony, Golden thyme, Thyme, White nanthene, Chiyu, and Ephedra, add each to 10 g of water, and heat to 100°C.
Extracted for 1 hour. Each water extract was diluted 1000 times to obtain an extract.

Fifty 30-day-old healthy pigs were used in each group, and the test group was given the extract in place of water, and the control group was given water.

The same basal feed as that used in Example 45 was used as the feed. The animals were raised in this manner for 60 days, and the number of dead animals and the number of animals with diarrhea due to field infection with Clostridium perfringens in each section were observed.

As a result, the number of dead animals and the number of animals with diarrhea were lower in all test plots than in the control plot.

Claims (1)

[Scope of Claims] 1) Licorice, mugwort, oren, scutellariae, scutellariae, gennoshoko, kouboku, tanjin, chimo, rhubarb, clove, jotei, keigai, keihi, genjin, ketsumeishi, biwayo, boufu, hop, soyou,
Youbaihi, Forsythia, Aloe, Gyutan, Ireisen, Ubai, Enmeiso, Shazenso, Shinii,
Chinko, Jinkou, Senkyu, Kouhon, Gobaishi, Cornelius, Shikon, Koouren, Peony,
A prophylactic and therapeutic agent for Clostridium perfringens infections in livestock and poultry, which contains one or more herbal medicines selected from the group consisting of goldenrod, thyme, whitenanthene, citrus and ephedra. 2) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is in the form of bulk powder. 3) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is an organic solvent, a mixture of water and an organic solvent, a crude drug extract extracted with water, and/or a crude drug active ingredient obtained therefrom. 4) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is blended into the feed. 5) The prophylactic and therapeutic agent according to claim 1, wherein the livestock and poultry are chickens, pigs, cows, and sheep. 6) Licorice, mugwort, oren, scutellariae, scutellariae, gennoshoko, kouboku, tanjin, chimo, rhubarb, clove, jotei, keigai, keihi, genjin, ketsumeishi, biwayo, boufu, hop, soybean,
Youbaihi, Forsythia, Aloe, Gyutan, Ireisen, Ubai, Enmeiso, Shazenso, Shinii,
Chinko, Jinkou, Senkyu, Kouhon, Gobaishi, Cornelius, Shikon, Koouren, Peony,
A method for preventing and treating Clostridium perfringens infections in livestock and poultry, which comprises administering an effective amount of one or more crude drugs selected from the group consisting of goldenrod, thyme, whitenanthene, citrus, and ephedra. 7) Claim 6 in which the crude drug is used in the form of bulk powder
Prevention and treatment methods described in Section. 8) The prevention and treatment method according to claim 6, wherein the herbal medicine is an organic solvent, a mixture of water and an organic solvent, a herbal medicine extract extracted with water, and/or an active herbal medicine ingredient obtained therefrom. 9) The prevention and treatment method according to claim 6, wherein the herbal medicine is mixed with feed and administered. 10) The prevention and treatment method according to claim 6, wherein the livestock and poultry are chickens, pigs, cows, and sheep.