JP7853291B2 - 複数のインサートを含む配列決定鋳型、並びに配列決定スループットを改善するための組成物及び方法 - Google Patents
複数のインサートを含む配列決定鋳型、並びに配列決定スループットを改善するための組成物及び方法Info
- Publication number
- JP7853291B2 JP7853291B2 JP2023524116A JP2023524116A JP7853291B2 JP 7853291 B2 JP7853291 B2 JP 7853291B2 JP 2023524116 A JP2023524116 A JP 2023524116A JP 2023524116 A JP2023524116 A JP 2023524116A JP 7853291 B2 JP7853291 B2 JP 7853291B2
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- Prior art keywords
- sequence
- insert
- sequencing
- adapter
- polynucleotide
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/101—Crosslinking agents, e.g. psoralen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063094422P | 2020-10-21 | 2020-10-21 | |
| US63/094,422 | 2020-10-21 | ||
| US202163256040P | 2021-10-15 | 2021-10-15 | |
| US63/256,040 | 2021-10-15 | ||
| PCT/US2021/055878 WO2022087150A2 (en) | 2020-10-21 | 2021-10-20 | Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023547366A JP2023547366A (ja) | 2023-11-10 |
| JP2023547366A5 JP2023547366A5 (https=) | 2024-10-29 |
| JP7853291B2 true JP7853291B2 (ja) | 2026-04-28 |
Family
ID=78622058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023524116A Active JP7853291B2 (ja) | 2020-10-21 | 2021-10-20 | 複数のインサートを含む配列決定鋳型、並びに配列決定スループットを改善するための組成物及び方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230407388A1 (https=) |
| EP (1) | EP4232600A2 (https=) |
| JP (1) | JP7853291B2 (https=) |
| KR (1) | KR20230091116A (https=) |
| CN (1) | CN116438319A (https=) |
| AU (1) | AU2021366658A1 (https=) |
| CA (1) | CA3198842A1 (https=) |
| IL (1) | IL302207A (https=) |
| MX (1) | MX2023004461A (https=) |
| WO (1) | WO2022087150A2 (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11427855B1 (en) | 2021-06-17 | 2022-08-30 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| KR20240047357A (ko) * | 2021-06-17 | 2024-04-12 | 엘리먼트 바이오사이언스, 인크. | 쌍별 서열분석을 위한 조성물 및 방법 |
| WO2022266470A1 (en) * | 2021-06-17 | 2022-12-22 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| JP2025507763A (ja) * | 2022-03-01 | 2025-03-21 | ガーダント ヘルス， インコーポレイテッド | シトシンのメチル化およびヒドロキシメチル化を分析するための方法 |
| AU2023234670A1 (en) | 2022-03-15 | 2024-01-18 | Illumina, Inc. | Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection |
| AU2023236924A1 (en) | 2022-03-15 | 2024-10-24 | Illumina, Inc. | Paired-end sequencing |
| US20250327063A1 (en) * | 2022-05-26 | 2025-10-23 | Illumina, Inc. | Preparation of long read nucleic acid libraries |
| EP4573213A1 (en) | 2022-08-15 | 2025-06-25 | Element Biosciences, Inc. | Spatially resolved surface capture of nucleic acids |
| KR20250075532A (ko) | 2022-09-19 | 2025-05-28 | 일루미나, 인코포레이티드 | 고정된 프라이머를 포함하는 변형가능한 중합체 |
| KR20260026601A (ko) * | 2023-04-13 | 2026-02-26 | 아니링 에스엘. | Dna 시퀀싱 방법 |
| WO2024256580A1 (en) | 2023-06-14 | 2024-12-19 | Illumina, Inc. | Concurrent sequencing with spatially separated rings |
| EP4728092A1 (en) | 2023-06-14 | 2026-04-22 | Illumina, Inc. | Determination of modified cytosines |
| WO2025062002A1 (en) | 2023-09-20 | 2025-03-27 | Illumina, Inc. | Concurrent sequencing using nick translation |
| WO2025062001A1 (en) | 2023-09-20 | 2025-03-27 | Illumina, Inc. | Optimised nucleic acid sequencing |
| WO2026006746A2 (en) * | 2024-06-28 | 2026-01-02 | Illumina, Inc. | Nucleic acid preparation and analysis techniques |
| WO2026006622A2 (en) * | 2024-06-28 | 2026-01-02 | Illumina, Inc. | Sequencing full-complement polynucleotides using nanopores |
Citations (7)
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| JP2017522866A (ja) | 2014-06-26 | 2017-08-17 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列の分析 |
| JP2018501776A (ja) | 2014-10-17 | 2018-01-25 | イルミナ ケンブリッジ リミテッド | 連続性を維持した転位 |
| JP2018508235A (ja) | 2015-02-17 | 2018-03-29 | コンプリート・ゲノミックス・インコーポレーテッド | 制御された鎖置換を用いてのｄｎａ配列決定 |
| JP2018537955A (ja) | 2015-10-09 | 2018-12-27 | アキュラジェン ホールディングス リミテッド | 増幅産生物の富化のための方法および組成物 |
| JP2020501554A (ja) | 2016-12-16 | 2020-01-23 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 短いｄｎａ断片を連結することによる一分子シーケンスのスループットを増加する方法 |
| JP2020510401A (ja) | 2017-02-21 | 2020-04-09 | イルミナ インコーポレイテッド | リンカーを用いた固定化トランスポソームを使用するタグメンテーション |
| JP2020103230A (ja) | 2018-12-28 | 2020-07-09 | 東洋紡株式会社 | アダプターダイマーの生成抑制方法 |
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| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
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| WO2020014437A1 (en) | 2018-07-12 | 2020-01-16 | Levine Alison | Modular apparel |
-
2021
- 2021-10-20 KR KR1020237016082A patent/KR20230091116A/ko active Pending
- 2021-10-20 IL IL302207A patent/IL302207A/en unknown
- 2021-10-20 CN CN202180071179.8A patent/CN116438319A/zh active Pending
- 2021-10-20 JP JP2023524116A patent/JP7853291B2/ja active Active
- 2021-10-20 AU AU2021366658A patent/AU2021366658A1/en active Pending
- 2021-10-20 MX MX2023004461A patent/MX2023004461A/es unknown
- 2021-10-20 CA CA3198842A patent/CA3198842A1/en active Pending
- 2021-10-20 WO PCT/US2021/055878 patent/WO2022087150A2/en not_active Ceased
- 2021-10-20 EP EP21807406.0A patent/EP4232600A2/en active Pending
-
2023
- 2023-04-20 US US18/303,905 patent/US20230407388A1/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017522866A (ja) | 2014-06-26 | 2017-08-17 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列の分析 |
| JP2018501776A (ja) | 2014-10-17 | 2018-01-25 | イルミナ ケンブリッジ リミテッド | 連続性を維持した転位 |
| JP2018508235A (ja) | 2015-02-17 | 2018-03-29 | コンプリート・ゲノミックス・インコーポレーテッド | 制御された鎖置換を用いてのｄｎａ配列決定 |
| JP2018537955A (ja) | 2015-10-09 | 2018-12-27 | アキュラジェン ホールディングス リミテッド | 増幅産生物の富化のための方法および組成物 |
| JP2020501554A (ja) | 2016-12-16 | 2020-01-23 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 短いｄｎａ断片を連結することによる一分子シーケンスのスループットを増加する方法 |
| JP2020510401A (ja) | 2017-02-21 | 2020-04-09 | イルミナ インコーポレイテッド | リンカーを用いた固定化トランスポソームを使用するタグメンテーション |
| JP2020103230A (ja) | 2018-12-28 | 2020-07-09 | 東洋紡株式会社 | アダプターダイマーの生成抑制方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4232600A2 (en) | 2023-08-30 |
| US20230407388A1 (en) | 2023-12-21 |
| AU2021366658A1 (en) | 2023-06-22 |
| WO2022087150A2 (en) | 2022-04-28 |
| AU2021366658A9 (en) | 2024-05-02 |
| CN116438319A (zh) | 2023-07-14 |
| MX2023004461A (es) | 2023-05-03 |
| KR20230091116A (ko) | 2023-06-22 |
| JP2023547366A (ja) | 2023-11-10 |
| WO2022087150A3 (en) | 2022-06-30 |
| IL302207A (en) | 2023-06-01 |
| CA3198842A1 (en) | 2022-04-28 |
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