AU2021366658A1 - Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput - Google Patents
Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput Download PDFInfo
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- AU2021366658A1 AU2021366658A1 AU2021366658A AU2021366658A AU2021366658A1 AU 2021366658 A1 AU2021366658 A1 AU 2021366658A1 AU 2021366658 A AU2021366658 A AU 2021366658A AU 2021366658 A AU2021366658 A AU 2021366658A AU 2021366658 A1 AU2021366658 A1 AU 2021366658A1
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- Australia
- Prior art keywords
- sequence
- complement
- nucleic acid
- insert
- sequencing
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/101—Crosslinking agents, e.g. psoralen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063094422P | 2020-10-21 | 2020-10-21 | |
| US63/094,422 | 2020-10-21 | ||
| US202163256040P | 2021-10-15 | 2021-10-15 | |
| US63/256,040 | 2021-10-15 | ||
| PCT/US2021/055878 WO2022087150A2 (en) | 2020-10-21 | 2021-10-20 | Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2021366658A1 true AU2021366658A1 (en) | 2023-06-22 |
| AU2021366658A9 AU2021366658A9 (en) | 2024-05-02 |
Family
ID=78622058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021366658A Pending AU2021366658A1 (en) | 2020-10-21 | 2021-10-20 | Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230407388A1 (https=) |
| EP (1) | EP4232600A2 (https=) |
| JP (1) | JP7853291B2 (https=) |
| KR (1) | KR20230091116A (https=) |
| CN (1) | CN116438319A (https=) |
| AU (1) | AU2021366658A1 (https=) |
| CA (1) | CA3198842A1 (https=) |
| IL (1) | IL302207A (https=) |
| MX (1) | MX2023004461A (https=) |
| WO (1) | WO2022087150A2 (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11427855B1 (en) | 2021-06-17 | 2022-08-30 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| KR20240047357A (ko) * | 2021-06-17 | 2024-04-12 | 엘리먼트 바이오사이언스, 인크. | 쌍별 서열분석을 위한 조성물 및 방법 |
| WO2022266470A1 (en) * | 2021-06-17 | 2022-12-22 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| JP2025507763A (ja) * | 2022-03-01 | 2025-03-21 | ガーダント ヘルス， インコーポレイテッド | シトシンのメチル化およびヒドロキシメチル化を分析するための方法 |
| AU2023234670A1 (en) | 2022-03-15 | 2024-01-18 | Illumina, Inc. | Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection |
| AU2023236924A1 (en) | 2022-03-15 | 2024-10-24 | Illumina, Inc. | Paired-end sequencing |
| US20250327063A1 (en) * | 2022-05-26 | 2025-10-23 | Illumina, Inc. | Preparation of long read nucleic acid libraries |
| EP4573213A1 (en) | 2022-08-15 | 2025-06-25 | Element Biosciences, Inc. | Spatially resolved surface capture of nucleic acids |
| KR20250075532A (ko) | 2022-09-19 | 2025-05-28 | 일루미나, 인코포레이티드 | 고정된 프라이머를 포함하는 변형가능한 중합체 |
| KR20260026601A (ko) * | 2023-04-13 | 2026-02-26 | 아니링 에스엘. | Dna 시퀀싱 방법 |
| WO2024256580A1 (en) | 2023-06-14 | 2024-12-19 | Illumina, Inc. | Concurrent sequencing with spatially separated rings |
| EP4728092A1 (en) | 2023-06-14 | 2026-04-22 | Illumina, Inc. | Determination of modified cytosines |
| WO2025062002A1 (en) | 2023-09-20 | 2025-03-27 | Illumina, Inc. | Concurrent sequencing using nick translation |
| WO2025062001A1 (en) | 2023-09-20 | 2025-03-27 | Illumina, Inc. | Optimised nucleic acid sequencing |
| WO2026006746A2 (en) * | 2024-06-28 | 2026-01-02 | Illumina, Inc. | Nucleic acid preparation and analysis techniques |
| WO2026006622A2 (en) * | 2024-06-28 | 2026-01-02 | Illumina, Inc. | Sequencing full-complement polynucleotides using nanopores |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| JP2002503954A (ja) | 1997-04-01 | 2002-02-05 | グラクソ、グループ、リミテッド | 核酸増幅法 |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| EP1701785A1 (en) | 2004-01-07 | 2006-09-20 | Solexa Ltd. | Modified molecular arrays |
| CA2579150C (en) | 2004-09-17 | 2014-11-25 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
| GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| CA2648149A1 (en) | 2006-03-31 | 2007-11-01 | Solexa, Inc. | Systems and devices for sequence by synthesis analysis |
| ATE542921T1 (de) | 2006-04-18 | 2012-02-15 | Advanced Liquid Logic Inc | Pyrosequenzierung auf tröpfchenbasis |
| US8343746B2 (en) | 2006-10-23 | 2013-01-01 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| WO2010127304A2 (en) | 2009-05-01 | 2010-11-04 | Illumina, Inc. | Sequencing methods |
| EP2633069B1 (en) | 2010-10-26 | 2015-07-01 | Illumina, Inc. | Sequencing methods |
| US9644199B2 (en) | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| CN105358715B (zh) | 2013-07-03 | 2018-09-18 | 伊鲁米那股份有限公司 | 正交合成测序 |
| MX370616B (es) * | 2013-11-22 | 2019-12-17 | Theranos Ip Co Llc | Amplificacion de acidos nucleicos. |
| WO2017062863A1 (en) * | 2015-10-09 | 2017-04-13 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| EP3957750B1 (en) | 2013-12-20 | 2025-01-29 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic dna samples |
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| US10975371B2 (en) | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
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| JP6773687B2 (ja) * | 2015-02-17 | 2020-10-21 | エムジーアイ テック カンパニー リミテッドＭＧＩ Ｔｅｃｈ Ｃｏ．， Ｌｔｄ． | 制御された鎖置換を用いてのｄｎａ配列決定 |
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| WO2018108328A1 (en) * | 2016-12-16 | 2018-06-21 | F. Hoffmann-La Roche Ag | Method for increasing throughput of single molecule sequencing by concatenating short dna fragments |
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| KR102866805B1 (ko) * | 2017-02-21 | 2025-09-30 | 일루미나, 인코포레이티드 | 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화 |
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| US20180346963A1 (en) * | 2017-06-01 | 2018-12-06 | Counsyl, Inc. | Preparation of Concatenated Polynucleotides |
| US11447818B2 (en) | 2017-09-15 | 2022-09-20 | Illumina, Inc. | Universal short adapters with variable length non-random unique molecular identifiers |
| CN110870016B (zh) | 2017-11-30 | 2024-09-06 | 伊鲁米那股份有限公司 | 用于序列变体呼出的验证方法和系统 |
| WO2020014437A1 (en) | 2018-07-12 | 2020-01-16 | Levine Alison | Modular apparel |
| JP7268352B2 (ja) * | 2018-12-28 | 2023-05-08 | 東洋紡株式会社 | アダプターダイマーの生成抑制方法 |
-
2021
- 2021-10-20 KR KR1020237016082A patent/KR20230091116A/ko active Pending
- 2021-10-20 IL IL302207A patent/IL302207A/en unknown
- 2021-10-20 CN CN202180071179.8A patent/CN116438319A/zh active Pending
- 2021-10-20 JP JP2023524116A patent/JP7853291B2/ja active Active
- 2021-10-20 AU AU2021366658A patent/AU2021366658A1/en active Pending
- 2021-10-20 MX MX2023004461A patent/MX2023004461A/es unknown
- 2021-10-20 CA CA3198842A patent/CA3198842A1/en active Pending
- 2021-10-20 WO PCT/US2021/055878 patent/WO2022087150A2/en not_active Ceased
- 2021-10-20 EP EP21807406.0A patent/EP4232600A2/en active Pending
-
2023
- 2023-04-20 US US18/303,905 patent/US20230407388A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4232600A2 (en) | 2023-08-30 |
| US20230407388A1 (en) | 2023-12-21 |
| WO2022087150A2 (en) | 2022-04-28 |
| JP7853291B2 (ja) | 2026-04-28 |
| AU2021366658A9 (en) | 2024-05-02 |
| CN116438319A (zh) | 2023-07-14 |
| MX2023004461A (es) | 2023-05-03 |
| KR20230091116A (ko) | 2023-06-22 |
| JP2023547366A (ja) | 2023-11-10 |
| WO2022087150A3 (en) | 2022-06-30 |
| IL302207A (en) | 2023-06-01 |
| CA3198842A1 (en) | 2022-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| SREP | Specification republished |