JP7846625B2 - 組換えａａｖの産生 - Google Patents

組換えａａｖの産生

Info

Publication number
JP7846625B2
JP7846625B2 JP2022543381A JP2022543381A JP7846625B2 JP 7846625 B2 JP7846625 B2 JP 7846625B2 JP 2022543381 A JP2022543381 A JP 2022543381A JP 2022543381 A JP2022543381 A JP 2022543381A JP 7846625 B2 JP7846625 B2 JP 7846625B2
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JP
Japan
Prior art keywords
aav
approximately
raav
cells
certain embodiments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022543381A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023510590A (ja
JPWO2021146591A5 (https=
JP2023510590A5 (https=
Inventor
ジェイコブ スミス
ジョシュ グリーガー
Original Assignee
アスクバイオ インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アスクバイオ インコーポレイテッド filed Critical アスクバイオ インコーポレイテッド
Publication of JP2023510590A publication Critical patent/JP2023510590A/ja
Publication of JPWO2021146591A5 publication Critical patent/JPWO2021146591A5/ja
Publication of JP2023510590A5 publication Critical patent/JP2023510590A5/ja
Priority to JP2025195186A priority Critical patent/JP2026032042A/ja
Application granted granted Critical
Publication of JP7846625B2 publication Critical patent/JP7846625B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
JP2022543381A 2020-01-17 2021-01-15 組換えａａｖの産生 Active JP7846625B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025195186A JP2026032042A (ja) 2020-01-17 2025-11-14 組換えａａｖの産生

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062962911P 2020-01-17 2020-01-17
US62/962,911 2020-01-17
PCT/US2021/013689 WO2021146591A2 (en) 2020-01-17 2021-01-15 Recombinant aav production

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025195186A Division JP2026032042A (ja) 2020-01-17 2025-11-14 組換えａａｖの産生

Publications (4)

Publication Number Publication Date
JP2023510590A JP2023510590A (ja) 2023-03-14
JPWO2021146591A5 JPWO2021146591A5 (https=) 2024-01-23
JP2023510590A5 JP2023510590A5 (https=) 2024-01-23
JP7846625B2 true JP7846625B2 (ja) 2026-04-15

Family

ID=76864730

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022543381A Active JP7846625B2 (ja) 2020-01-17 2021-01-15 組換えａａｖの産生
JP2025195186A Pending JP2026032042A (ja) 2020-01-17 2025-11-14 組換えａａｖの産生

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025195186A Pending JP2026032042A (ja) 2020-01-17 2025-11-14 組換えａａｖの産生

Country Status (8)

Country Link
US (1) US20230048994A1 (https=)
EP (1) EP4090750A4 (https=)
JP (2) JP7846625B2 (https=)
CN (1) CN115315518A (https=)
AU (1) AU2021207683A1 (https=)
CA (1) CA3162520A1 (https=)
IL (1) IL294775A (https=)
WO (1) WO2021146591A2 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4189077A1 (en) 2020-07-30 2023-06-07 Shape Therapeutics Inc. Stable cell lines for inducible production of raav virions
AU2022373653A1 (en) 2021-10-18 2024-05-02 Regeneron Pharmaceuticals, Inc. Eukaryotic cells comprising adenovirus-associated virus polynucleotides
WO2024129882A1 (en) * 2022-12-14 2024-06-20 Astellas Gene Therapies, Inc. Compositions and methods for improved production of adeno-associated viral particles
AU2024223271A1 (en) * 2023-02-17 2025-09-18 Synplogen Co., Ltd. Itr-rep gene complex
EP4527938A1 (en) * 2023-09-22 2025-03-26 LenioBio GmbH Method for cell-free polypeptide synthesis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015031686A1 (en) 2013-08-30 2015-03-05 Amgen Inc. High titer recombinant aav vector production in adherent and suspension cells
WO2019246544A2 (en) 2018-06-22 2019-12-26 Asklepios Biopharmaceutical, Inc. Vectors for gene delivery that persist within cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6995006B2 (en) * 1997-09-05 2006-02-07 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
EP2848253A1 (en) * 2006-06-19 2015-03-18 Asklepios Biopharmaceutical, Inc. Modified factor VIII and factor IX genes and vectors for gene therapy
WO2008105931A2 (en) * 2006-09-15 2008-09-04 Medimmune Vaccines, Inc. Mdck cell lines supporting viral growth to high titers and bioreactor process using the same
GB0901593D0 (en) * 2009-01-30 2009-03-11 Touchlight Genetics Ltd Production of closed linear DNA
US9441206B2 (en) * 2011-10-28 2016-09-13 The University Of North Carolina At Chapel Hill Cell line for production of adeno-associated virus
AU2016362317B2 (en) * 2015-12-01 2023-03-16 Spark Therapeutics, Inc. Scalable methods for producing recombinant Adeno-Associated Viral (AAV) vector in serum-free suspension cell culture system suitable for clinical use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015031686A1 (en) 2013-08-30 2015-03-05 Amgen Inc. High titer recombinant aav vector production in adherent and suspension cells
WO2019246544A2 (en) 2018-06-22 2019-12-26 Asklepios Biopharmaceutical, Inc. Vectors for gene delivery that persist within cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CELL & GENE THERAPY INSIGHTS，2017年，pp.731-738，DOI: 10.18609/cgti.2017.074

Also Published As

Publication number Publication date
WO2021146591A3 (en) 2021-10-28
CN115315518A (zh) 2022-11-08
JP2026032042A (ja) 2026-02-25
JP2023510590A (ja) 2023-03-14
EP4090750A4 (en) 2024-03-13
AU2021207683A1 (en) 2022-08-11
US20230048994A1 (en) 2023-02-16
CA3162520A1 (en) 2021-07-22
EP4090750A2 (en) 2022-11-23
IL294775A (en) 2022-09-01
WO2021146591A2 (en) 2021-07-22

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