AU2021207683A1 - Recombinant AAV production - Google Patents
Recombinant AAV production Download PDFInfo
- Publication number
- AU2021207683A1 AU2021207683A1 AU2021207683A AU2021207683A AU2021207683A1 AU 2021207683 A1 AU2021207683 A1 AU 2021207683A1 AU 2021207683 A AU2021207683 A AU 2021207683A AU 2021207683 A AU2021207683 A AU 2021207683A AU 2021207683 A1 AU2021207683 A1 AU 2021207683A1
- Authority
- AU
- Australia
- Prior art keywords
- aav
- nucleic acid
- raav
- cell line
- certain embodiments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062962911P | 2020-01-17 | 2020-01-17 | |
| US62/962,911 | 2020-01-17 | ||
| PCT/US2021/013689 WO2021146591A2 (en) | 2020-01-17 | 2021-01-15 | Recombinant aav production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021207683A1 true AU2021207683A1 (en) | 2022-08-11 |
Family
ID=76864730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021207683A Pending AU2021207683A1 (en) | 2020-01-17 | 2021-01-15 | Recombinant AAV production |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20230048994A1 (https=) |
| EP (1) | EP4090750A4 (https=) |
| JP (2) | JP7846625B2 (https=) |
| CN (1) | CN115315518A (https=) |
| AU (1) | AU2021207683A1 (https=) |
| CA (1) | CA3162520A1 (https=) |
| IL (1) | IL294775A (https=) |
| WO (1) | WO2021146591A2 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12054738B2 (en) | 2020-07-30 | 2024-08-06 | Shape Therapeutics Inc. | Stable cell lines for inducible production of rAAV virions |
| TW202323529A (zh) | 2021-10-18 | 2023-06-16 | 美商再生元醫藥公司 | 包括腺病毒相關病毒聚核苷酸的真核細胞 |
| EP4634370A1 (en) * | 2022-12-14 | 2025-10-22 | Astellas Gene Therapies, Inc. | Compositions and methods for improved production of adeno-associated viral particles |
| CN120752344A (zh) * | 2023-02-17 | 2025-10-03 | 新博基股份有限公司 | ITR-Rep基因复合体 |
| EP4527938A1 (en) * | 2023-09-22 | 2025-03-26 | LenioBio GmbH | Method for cell-free polypeptide synthesis |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6989264B2 (en) * | 1997-09-05 | 2006-01-24 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
| ES2535877T3 (es) * | 2006-06-19 | 2015-05-18 | Asklepios Biopharmaceutical, Inc. | Genes de Factor VIII y Factor IX modificados y vectores para la terapia génica |
| EP2615167A1 (en) * | 2006-09-15 | 2013-07-17 | MedImmune, LLC | Method for eliminating DNA contaminants from viral preparations |
| GB0901593D0 (en) * | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| CA2853482C (en) * | 2011-10-28 | 2020-05-19 | Joshua Grieger | Cell line for production of adeno-associated virus |
| WO2015031686A1 (en) * | 2013-08-30 | 2015-03-05 | Amgen Inc. | High titer recombinant aav vector production in adherent and suspension cells |
| EP3384015A4 (en) * | 2015-12-01 | 2019-05-29 | Spark Therapeutics, Inc. | SCALABLE PROCESS FOR THE PREPARATION OF A RECOMBINANT ADENO ASSOCIATED VIRAL VECTOR IN A SERUM-FREE SUSPENSION CELL CULTURE SYSTEM SUITABLE FOR THE CLINICAL APPLICATION |
| EP3810782A2 (en) | 2018-06-22 | 2021-04-28 | Asklepios Biopharmaceutical, Inc. | Vectors for gene delivery that persist within cells |
-
2021
- 2021-01-15 CN CN202180023263.2A patent/CN115315518A/zh active Pending
- 2021-01-15 JP JP2022543381A patent/JP7846625B2/ja active Active
- 2021-01-15 EP EP21741128.9A patent/EP4090750A4/en active Pending
- 2021-01-15 IL IL294775A patent/IL294775A/en unknown
- 2021-01-15 CA CA3162520A patent/CA3162520A1/en active Pending
- 2021-01-15 WO PCT/US2021/013689 patent/WO2021146591A2/en not_active Ceased
- 2021-01-15 US US17/793,196 patent/US20230048994A1/en active Pending
- 2021-01-15 AU AU2021207683A patent/AU2021207683A1/en active Pending
-
2025
- 2025-11-14 JP JP2025195186A patent/JP2026032042A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20230048994A1 (en) | 2023-02-16 |
| IL294775A (en) | 2022-09-01 |
| CN115315518A (zh) | 2022-11-08 |
| EP4090750A4 (en) | 2024-03-13 |
| EP4090750A2 (en) | 2022-11-23 |
| WO2021146591A2 (en) | 2021-07-22 |
| CA3162520A1 (en) | 2021-07-22 |
| WO2021146591A3 (en) | 2021-10-28 |
| JP2026032042A (ja) | 2026-02-25 |
| JP2023510590A (ja) | 2023-03-14 |
| JP7846625B2 (ja) | 2026-04-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20230048994A1 (en) | Recombinant aav production | |
| CN104520421B (zh) | 用于生产腺伴随病毒的细胞系 | |
| US20240084268A1 (en) | Method for purifying recombinant viral particles | |
| JP2001514845A (ja) | 組換えａａｖベクターの高力価ヘルパーなし調製物を生成するための方法 | |
| JP2024538792A (ja) | 無血清浮遊培養に適したｈｅｋ２９３細胞株及びその応用 | |
| US20240279682A1 (en) | AAV Manufacturing Methods | |
| US20250263746A1 (en) | Method for producing recombinant aav particles | |
| KR20250162543A (ko) | 재조합 aav 입자 제제 생산 방법 | |
| US20250136651A1 (en) | Recombinant optimized mecp2 cassettes and methods for treating rett syndrome and related disorders | |
| HK40081423A (en) | Recombinant aav production | |
| HK40112977A (zh) | 用於确定aav基因组的方法 | |
| HK40117756A (zh) | 用於生产重组aav颗粒的方法 | |
| JP2025516117A (ja) | Ａａｖゲノムを決定するための方法 | |
| WO2025168663A1 (en) | Method for producing recombinant adeno-associated viral particles | |
| TW202405182A (zh) | 生產重組ａａｖ顆粒之方法 | |
| HK40129257A (zh) | 用於产生重组aav颗粒制备物的方法 | |
| WO2022125601A1 (en) | Cell lines for production of adeno-associated virus | |
| CN119569833A (zh) | 靶向骨骼肌的aav衣壳蛋白变体及其用途 |