JP7777830B2 - フェニルアラニンアンモニアリアーゼを用いた鎖状の不飽和カルボン酸化合物の製造方法 - Google Patents

フェニルアラニンアンモニアリアーゼを用いた鎖状の不飽和カルボン酸化合物の製造方法

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Publication number
JP7777830B2
JP7777830B2 JP2022572955A JP2022572955A JP7777830B2 JP 7777830 B2 JP7777830 B2 JP 7777830B2 JP 2022572955 A JP2022572955 A JP 2022572955A JP 2022572955 A JP2022572955 A JP 2022572955A JP 7777830 B2 JP7777830 B2 JP 7777830B2
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amino acid
group
carbon atoms
seq
set forth
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JPWO2022145178A1 (https=
Inventor
智量 白井
裕太郎 森
義秀 谷地
操 日座
弥生 赤堀
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Zeon Corp
Yokohama Rubber Co Ltd
RIKEN
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Zeon Corp
Yokohama Rubber Co Ltd
RIKEN
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01024—Phenylalanine ammonia-lyase (4.3.1.24)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2022572955A 2020-12-28 2021-12-07 フェニルアラニンアンモニアリアーゼを用いた鎖状の不飽和カルボン酸化合物の製造方法 Active JP7777830B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020219017 2020-12-28
JP2020219017 2020-12-28
PCT/JP2021/044890 WO2022145178A1 (ja) 2020-12-28 2021-12-07 フェニルアラニンアンモニアリアーゼを用いた鎖状の不飽和カルボン酸化合物の製造方法

Publications (2)

Publication Number Publication Date
JPWO2022145178A1 JPWO2022145178A1 (https=) 2022-07-07
JP7777830B2 true JP7777830B2 (ja) 2025-12-01

Family

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JP2022572955A Active JP7777830B2 (ja) 2020-12-28 2021-12-07 フェニルアラニンアンモニアリアーゼを用いた鎖状の不飽和カルボン酸化合物の製造方法

Country Status (5)

Country Link
US (1) US20250320481A1 (https=)
EP (1) EP4269593A4 (https=)
JP (1) JP7777830B2 (https=)
CN (1) CN116710549A (https=)
WO (1) WO2022145178A1 (https=)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012018624A2 (en) 2010-07-26 2012-02-09 Genomatica, Inc. Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
US20130005012A1 (en) 2011-06-23 2013-01-03 Phytogene, Inc. Enzymatic system for monomer synthesis
WO2013028519A1 (en) 2011-08-19 2013-02-28 Genomatica, Inc. Microorganisms and methods for producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol and related alcohols
JP2014525741A (ja) 2011-06-30 2014-10-02 インビスタ テクノロジーズ エス．アー．エール．エル． ナイロン−７、ナイロン−７，７、およびポリエステルを生産するための生物変換方法
US20150337336A1 (en) 2012-06-22 2015-11-26 Phytogene, Inc. Enzymes and methods for styrene synthesis
US20160230194A1 (en) 2013-09-18 2016-08-11 Arizona Board Of Regents On Behalf Of Arizona State University Enzymes and Microorganisms for the Production of 1,3-Butadiene and Other Dienes
WO2019022083A1 (ja) 2017-07-24 2019-01-31 国立研究開発法人理化学研究所 デカルボキシラーゼ、及びそれを用いた不飽和炭化水素化合物の製造方法
WO2020013951A1 (en) 2018-07-12 2020-01-16 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
US20200048674A1 (en) 2017-04-24 2020-02-13 The Regents Of The University Of California Biosynthesis of bioorthogonal amino acids

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014030376A (ja) 2012-08-02 2014-02-20 Mitsubishi Chemicals Corp ブタジエンの製造方法
JP2015228804A (ja) 2014-06-03 2015-12-21 三菱化学株式会社 ２，３−ブタンジオール生産能を有する微生物およびそれを用いた２，３−ブタンジオールの製造方法、１，３−ブタジエンの製造方法

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012018624A2 (en) 2010-07-26 2012-02-09 Genomatica, Inc. Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
US20130005012A1 (en) 2011-06-23 2013-01-03 Phytogene, Inc. Enzymatic system for monomer synthesis
JP2014525741A (ja) 2011-06-30 2014-10-02 インビスタ テクノロジーズ エス．アー．エール．エル． ナイロン−７、ナイロン−７，７、およびポリエステルを生産するための生物変換方法
WO2013028519A1 (en) 2011-08-19 2013-02-28 Genomatica, Inc. Microorganisms and methods for producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol and related alcohols
US20150337336A1 (en) 2012-06-22 2015-11-26 Phytogene, Inc. Enzymes and methods for styrene synthesis
US20160230194A1 (en) 2013-09-18 2016-08-11 Arizona Board Of Regents On Behalf Of Arizona State University Enzymes and Microorganisms for the Production of 1,3-Butadiene and Other Dienes
US20200048674A1 (en) 2017-04-24 2020-02-13 The Regents Of The University Of California Biosynthesis of bioorthogonal amino acids
WO2019022083A1 (ja) 2017-07-24 2019-01-31 国立研究開発法人理化学研究所 デカルボキシラーゼ、及びそれを用いた不飽和炭化水素化合物の製造方法
WO2020013951A1 (en) 2018-07-12 2020-01-16 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Alina Filip et al.，Tailored Mutants of Phenylalanine Ammonia-Lyase from Petroselinumcrispum for the Synthesis of Bulky l- and d-Arylalanines，ChemCatChem，2018年07月18日，Vol.10(12)，pp.2627-2633
Hanson, Kenneth R, et al.，Phenylalanine ammonia-lyase: enzymicconversion of 3-(1,4-cyclohexadienyl)-L-alanine totrans-3-(1,4-cyclohexadienyl)acrylic acid，Biochemistry，1979年，Vol.18(8)，pp.1431-1438

Also Published As

Publication number Publication date
EP4269593A4 (en) 2025-08-13
CN116710549A (zh) 2023-09-05
JPWO2022145178A1 (https=) 2022-07-07
US20250320481A1 (en) 2025-10-16
WO2022145178A1 (ja) 2022-07-07
EP4269593A1 (en) 2023-11-01

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