JP7749539B2 - 酵素的ｒｎａキャッピング法 - Google Patents

酵素的ｒｎａキャッピング法

Info

Publication number
JP7749539B2
JP7749539B2 JP2022512342A JP2022512342A JP7749539B2 JP 7749539 B2 JP7749539 B2 JP 7749539B2 JP 2022512342 A JP2022512342 A JP 2022512342A JP 2022512342 A JP2022512342 A JP 2022512342A JP 7749539 B2 JP7749539 B2 JP 7749539B2
Authority
JP
Japan
Prior art keywords
rna
capping
activity
cap
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022512342A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022544712A (ja
JP2022544712A5 (https=
Inventor
ロブ，ジー・ブレット
チャン，シュウ－ホン
ロイ，ビジョイータ
Original Assignee
ニユー・イングランド・バイオレイブス・インコーポレイテツド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ニユー・イングランド・バイオレイブス・インコーポレイテツド filed Critical ニユー・イングランド・バイオレイブス・インコーポレイテツド
Priority claimed from PCT/US2020/047533 external-priority patent/WO2021041267A1/en
Publication of JP2022544712A publication Critical patent/JP2022544712A/ja
Publication of JP2022544712A5 publication Critical patent/JP2022544712A5/ja
Priority to JP2025156838A priority Critical patent/JP2026001059A/ja
Application granted granted Critical
Publication of JP7749539B2 publication Critical patent/JP7749539B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01056—Methyltransferases (2.1.1) mRNA (guanine-N7-)-methyltransferase (2.1.1.56)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02003—Uridine phosphorylase (2.4.2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07045—Guanosine-triphosphate guanylyltransferase (2.7.7.45)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2022512342A 2019-08-23 2020-08-21 酵素的ｒｎａキャッピング法 Active JP7749539B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025156838A JP2026001059A (ja) 2019-08-23 2025-09-22 酵素的ｒｎａキャッピング法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962890821P 2019-08-23 2019-08-23
US62/890,821 2019-08-23
PCT/US2020/047533 WO2021041267A1 (en) 2019-08-23 2020-08-21 Enzymatic rna capping method
PCT/US2020/047521 WO2021041260A1 (en) 2019-08-23 2020-08-21 Enzymatic rna capping method
USPCT/US2020/047521 2020-08-21

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025156838A Division JP2026001059A (ja) 2019-08-23 2025-09-22 酵素的ｒｎａキャッピング法

Publications (3)

Publication Number Publication Date
JP2022544712A JP2022544712A (ja) 2022-10-20
JP2022544712A5 JP2022544712A5 (https=) 2023-08-25
JP7749539B2 true JP7749539B2 (ja) 2025-10-06

Family

ID=80856181

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022512342A Active JP7749539B2 (ja) 2019-08-23 2020-08-21 酵素的ｒｎａキャッピング法
JP2025156838A Pending JP2026001059A (ja) 2019-08-23 2025-09-22 酵素的ｒｎａキャッピング法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025156838A Pending JP2026001059A (ja) 2019-08-23 2025-09-22 酵素的ｒｎａキャッピング法

Country Status (5)

Country Link
US (5) US12479880B2 (https=)
JP (2) JP7749539B2 (https=)
KR (1) KR20220046693A (https=)
AU (1) AU2020336278A1 (https=)
BR (1) BR112022003517A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2026001059A (ja) * 2019-08-23 2026-01-06 ニユー・イングランド・バイオレイブス・インコーポレイテツド 酵素的ｒｎａキャッピング法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230148714A (ko) 2022-04-18 2023-10-25 에스케이바이오사이언스(주) 면역원성 조성물 및 이의 제조방법
WO2025164636A1 (ja) * 2024-01-30 2025-08-07 タカラバイオ株式会社 ファウストウイルスｓ１７株由来のｒｎａキャッピング酵素
WO2025263589A1 (ja) * 2024-06-20 2025-12-26 ヤマサ醤油株式会社 Ｒｎａの５'末端から１番目及び２番目のヌクレオチドにおけるリボースの水酸基をメチル化するためのメチル基転移酵素、メチル化方法、メチル基転移酵素の使用、及びキット

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170253911A1 (en) 2013-12-05 2017-09-07 New England Biolabs, Inc. Methods for Enriching for a Population of RNA Molecules
US20180237817A1 (en) 2015-05-29 2018-08-23 Curevac Ag Method for adding cap structures to rna using immobilized enzymes
WO2019020811A1 (en) 2017-07-27 2019-01-31 Eukarys NEW CHIMERIC ENZYMES AND THEIR APPLICATIONS

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2010659B1 (en) 2006-04-14 2014-06-18 CellScript, Inc. Kits and methods for generating 5' capped RNA
EP2377938A1 (en) 2010-04-16 2011-10-19 Eukarys Capping-prone RNA polymerase enzymes and their applications
AU2012236099A1 (en) 2011-03-31 2013-10-03 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
WO2014028429A2 (en) 2012-08-14 2014-02-20 Moderna Therapeutics, Inc. Enzymes and polymerases for the synthesis of rna
KR101422936B1 (ko) 2012-11-30 2014-07-23 삼성전기주식회사 모터 구동 제어 장치, 모터 구동 제어 방법 및 그를 이용한 모터
ES3032935T3 (en) 2013-10-22 2025-07-29 Translate Bio Inc Lipid formulations for delivery of messenger rna
EP3077406B1 (en) 2013-12-05 2019-07-10 New England Biolabs, Inc. Compositions and methods for capping rna
US11479766B2 (en) 2013-12-05 2022-10-25 New England Biolabs, Inc. Methods for labeling a population of RNA molecules
BR112016030852A2 (pt) 2014-07-02 2018-01-16 Shire Human Genetic Therapies encapsulação de rna mensageiro
CN116144626A (zh) 2016-01-13 2023-05-23 新英格兰生物实验室公司 T7 rna聚合酶的热稳定变体
US10034951B1 (en) 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
US12398173B2 (en) * 2019-08-23 2025-08-26 New England Biolabs, Inc. Enzymatic RNA capping method
JP7749539B2 (ja) * 2019-08-23 2025-10-06 ニユー・イングランド・バイオレイブス・インコーポレイテツド 酵素的ｒｎａキャッピング法
US11788074B2 (en) 2020-06-23 2023-10-17 New England Biolabs, Inc. Vaccinia capping enzyme compositions and methods

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170253911A1 (en) 2013-12-05 2017-09-07 New England Biolabs, Inc. Methods for Enriching for a Population of RNA Molecules
US20180237817A1 (en) 2015-05-29 2018-08-23 Curevac Ag Method for adding cap structures to rna using immobilized enzymes
WO2019020811A1 (en) 2017-07-27 2019-01-31 Eukarys NEW CHIMERIC ENZYMES AND THEIR APPLICATIONS

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2026001059A (ja) * 2019-08-23 2026-01-06 ニユー・イングランド・バイオレイブス・インコーポレイテツド 酵素的ｒｎａキャッピング法

Also Published As

Publication number Publication date
JP2026001059A (ja) 2026-01-06
JP2022544712A (ja) 2022-10-20
CA3147797A1 (en) 2021-03-04
US20250388618A1 (en) 2025-12-25
US12479880B2 (en) 2025-11-25
US20260062438A1 (en) 2026-03-05
AU2020336278A1 (en) 2022-04-07
US20240383939A1 (en) 2024-11-21
US20260062439A1 (en) 2026-03-05
US20260015379A1 (en) 2026-01-15
KR20220046693A (ko) 2022-04-14
BR112022003517A2 (pt) 2022-07-19

Similar Documents

Publication Publication Date Title
CN112714795B (zh) 酶促rna加帽方法
US12398173B2 (en) Enzymatic RNA capping method
JP7749539B2 (ja) 酵素的ｒｎａキャッピング法
US20220127667A1 (en) Method of dna synthesis
US7323310B2 (en) Methods and compositions for RNA amplification and detection using an RNA-dependent RNA-polymerase
US9005930B2 (en) Kits and methods for generating 5′ capped RNA
JP2024518546A (ja) 修飾されたｍＲＮＡ、修飾された非コードＲＮＡ、およびその使用
ES2960381T3 (es) Expresión acelular de proteínas utilizando ADN concatemérico bicatenario
CA3114892A1 (en) Methods and compositions for increasing capping efficiency of transcribed rna
KR20170040263A (ko) 열불안정성 엑소뉴클레아제
JP6074036B2 (ja) 拡大された基質範囲を有する新規のｄｎａポリメラーゼ
CA3147797C (en) Enzymatic rna capping method
KR20220025709A (ko) 등온 dna 증폭을 위한 방법 및 조성물
US12371725B2 (en) Synthesis of DNA molecules in in vitro enzymatic systems
US12492420B2 (en) Compositions, kits, and methods for in vitro transcription
Liu et al. Multifaceted mRNA analysis using programmed RNA cleavage by Mucilaginibacter paludis Argonaute
EP4689177A1 (en) Novel method for isothermal amplification of padlock probes for nucleic acid detection and phi29 dna polymerase variants

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230817

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230817

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240820

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241120

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250218

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250508

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250826

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250924

R150 Certificate of patent or registration of utility model

Ref document number: 7749539

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150