JP7665513B2 - 細胞を単離及び拡大する方法 - Google Patents

細胞を単離及び拡大する方法 Download PDF

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JP7665513B2
JP7665513B2 JP2021524452A JP2021524452A JP7665513B2 JP 7665513 B2 JP7665513 B2 JP 7665513B2 JP 2021524452 A JP2021524452 A JP 2021524452A JP 2021524452 A JP2021524452 A JP 2021524452A JP 7665513 B2 JP7665513 B2 JP 7665513B2
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hematopoietic tissue
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JP2022506841A5 (https=
JP2022506841A (ja
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バンダリ シュリスティ
フローレンス サミュエル
ハットン アンドリュー
マシアス ルイーザ
ヌスバウマー オリバー
ソダーストロム カレ
ウーデン マーク
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GammaDelta Therapeutics Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
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  • Wood Science & Technology (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Veterinary Medicine (AREA)
  • Clinical Laboratory Science (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2021524452A 2018-11-08 2019-11-08 細胞を単離及び拡大する方法 Active JP7665513B2 (ja)

Priority Applications (1)

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JP2025064036A JP2025106453A (ja) 2018-11-08 2025-04-09 細胞を単離及び拡大する方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1818243.6 2018-11-08
GBGB1818243.6A GB201818243D0 (en) 2018-11-08 2018-11-08 Methods for isolating and expanding cells
PCT/GB2019/053166 WO2020095059A1 (en) 2018-11-08 2019-11-08 Methods for isolating and expanding cells

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JP2025064036A Division JP2025106453A (ja) 2018-11-08 2025-04-09 細胞を単離及び拡大する方法

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JP2022506841A JP2022506841A (ja) 2022-01-17
JP2022506841A5 JP2022506841A5 (https=) 2022-11-25
JP7665513B2 true JP7665513B2 (ja) 2025-04-21

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JP2025064036A Pending JP2025106453A (ja) 2018-11-08 2025-04-09 細胞を単離及び拡大する方法

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US (1) US12460180B2 (https=)
EP (1) EP3877510A1 (https=)
JP (2) JP7665513B2 (https=)
KR (1) KR20210111747A (https=)
CN (1) CN113423821A (https=)
AU (1) AU2019374489B2 (https=)
BR (1) BR112021008166A2 (https=)
CA (1) CA3117913A1 (https=)
EA (1) EA202190864A1 (https=)
GB (1) GB201818243D0 (https=)
IL (1) IL282901A (https=)
MX (1) MX2021005480A (https=)
PH (1) PH12021551038A1 (https=)
WO (1) WO2020095059A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201818243D0 (en) 2018-11-08 2018-12-26 Gammadelta Therapeutics Ltd Methods for isolating and expanding cells
JP2022545196A (ja) 2019-08-16 2022-10-26 ガンマデルタ セラピューティクス リミテッド 抗ｔｃｒデルタ可変１抗体の治療的使用
JP7764364B2 (ja) 2019-08-16 2025-11-05 ガンマデルタ セラピューティクス リミティッド エクスビボγδ Ｔ細胞集団
GB202002581D0 (en) 2020-02-24 2020-04-08 Gammadelta Therapeutics Ltd Novel antibodies
PL4269444T3 (pl) 2020-08-14 2025-01-13 GammaDelta Therapeutics Limited Wieloswoiste przeciwciała anty-tcr zmienne delta 1
AU2022224391A1 (en) 2021-02-17 2023-08-24 Gammadelta Therapeutics Ltd Anti-tcr delta variable 1 antibodies
GB202105113D0 (en) 2021-04-09 2021-05-26 Gammadelta Therapeutics Ltd Novel method
CA3228168A1 (en) 2021-08-03 2023-02-09 Gammadelta Therapeutics Ltd Engineering of gamma delta t cells and compositions thereof
EP4504247A1 (en) 2022-04-04 2025-02-12 Gammadelta Therapeutics Ltd Novel gene armoring
GB202204926D0 (en) 2022-04-04 2022-05-18 Gammadelta Therapeutics Ltd Method for expanding gammadelta T cells
CN121532496A (zh) * 2023-07-20 2026-02-13 武田药品工业株式会社 用于制备γδT细胞的无网格方法

Citations (4)

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JP2007511205A (ja) 2003-10-08 2007-05-10 ウィルソン ウォルフ マニュファクチャリング コーポレイション 気体透過性物質を利用する細胞を培養する方法及び装置
JP2013524856A (ja) 2010-05-07 2013-06-20 ファイブロセル テクノロジーズ， インコーポレイテッド 自己真皮線維芽細胞の投与単位処方物
US20180312808A1 (en) 2015-10-30 2018-11-01 Cancer Research Technology Limited Expansion of non-haematopoietic tissue-resident gamma delta t cells and uses of these cells
WO2018202808A2 (en) 2017-05-03 2018-11-08 King's College, London Expansion of gamma delta t cells, compositions, and methods of use thereof

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US6028176A (en) 1996-07-19 2000-02-22 Bayer Corporation High-affinity interleukin-4 muteins
US7078034B2 (en) 1999-01-28 2006-07-18 Palmetto Health Alliance In vitro activated γ δ lymphocytes
EP1777294A1 (en) 2005-10-20 2007-04-25 Institut National De La Sante Et De La Recherche Medicale (Inserm) IL-15Ralpha sushi domain as a selective and potent enhancer of IL-15 action through IL-15Rbeta/gamma, and hyperagonist (IL15Ralpha sushi -IL15) fusion proteins
WO2007111714A2 (en) 2005-11-28 2007-10-04 Zymogenetics, Inc. Il-21 antagonists
JP4659785B2 (ja) * 2007-06-15 2011-03-30 トヨタ自動車株式会社 内燃機関の燃料噴射制御装置
CA2860170C (en) 2010-12-22 2022-06-14 The Board Of Trustees Of The Leland Stanford Junior University Superagonists and antagonists of interleukin-2
PT2710123T (pt) 2011-05-19 2017-10-13 Inst De Medicina Molecular Linha celular de linfócitos compreendendo células t gama-delta, método de composição e produção resultante dos mesmos
EP3060059A4 (en) 2013-10-25 2017-11-01 Board of Regents, The University of Texas System Polyclonal gamma delta t cells for immunotherapy
RU2739770C2 (ru) 2014-06-11 2020-12-28 полибайосепт ГмбХ Экспансия лимфоцитов с использованием композиции цитокинов для активной клеточной иммунотерапии
IL297773B2 (en) 2014-11-17 2024-07-01 Adicet Bio Inc Engineered gamma delta t-cells
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JP2007511205A (ja) 2003-10-08 2007-05-10 ウィルソン ウォルフ マニュファクチャリング コーポレイション 気体透過性物質を利用する細胞を培養する方法及び装置
JP2013524856A (ja) 2010-05-07 2013-06-20 ファイブロセル テクノロジーズ， インコーポレイテッド 自己真皮線維芽細胞の投与単位処方物
US20180312808A1 (en) 2015-10-30 2018-11-01 Cancer Research Technology Limited Expansion of non-haematopoietic tissue-resident gamma delta t cells and uses of these cells
WO2018202808A2 (en) 2017-05-03 2018-11-08 King's College, London Expansion of gamma delta t cells, compositions, and methods of use thereof

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RACHAEL A. CLARK; ET AL，A NOVEL METHOD FOR THE ISOLATION OF SKIN RESIDENT T CELLS FROM NORMAL AND DISEASED HUMAN SKIN，JOURNAL OF INVESTIGATIVE DERMATOLOGY，NL，2006年05月，VOL:126, NR:5，PAGE(S):1059-1070，https://www.jidonline.org/action/showPdf?pii=S0022-202X%2815%2932866-9

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Publication number Publication date
US20220010276A1 (en) 2022-01-13
WO2020095059A1 (en) 2020-05-14
MX2021005480A (es) 2021-11-04
CA3117913A1 (en) 2020-05-14
BR112021008166A2 (pt) 2021-08-03
US12460180B2 (en) 2025-11-04
EP3877510A1 (en) 2021-09-15
EA202190864A1 (ru) 2021-09-14
IL282901A (en) 2021-06-30
KR20210111747A (ko) 2021-09-13
JP2025106453A (ja) 2025-07-15
PH12021551038A1 (en) 2022-01-03
AU2019374489B2 (en) 2026-04-02
JP2022506841A (ja) 2022-01-17
CN113423821A (zh) 2021-09-21
GB201818243D0 (en) 2018-12-26
AU2019374489A1 (en) 2021-05-27

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