BR112021008166A2 - métodos para isolamento e expansão de células - Google Patents
métodos para isolamento e expansão de células Download PDFInfo
- Publication number
- BR112021008166A2 BR112021008166A2 BR112021008166-6A BR112021008166A BR112021008166A2 BR 112021008166 A2 BR112021008166 A2 BR 112021008166A2 BR 112021008166 A BR112021008166 A BR 112021008166A BR 112021008166 A2 BR112021008166 A2 BR 112021008166A2
- Authority
- BR
- Brazil
- Prior art keywords
- cells
- population
- hematopoietic tissue
- tissue sample
- lymphocytes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
- C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2302—Interleukin-2 (IL-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2315—Interleukin-15 (IL-15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Clinical Laboratory Science (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1818243.6 | 2018-11-08 | ||
| GBGB1818243.6A GB201818243D0 (en) | 2018-11-08 | 2018-11-08 | Methods for isolating and expanding cells |
| PCT/GB2019/053166 WO2020095059A1 (en) | 2018-11-08 | 2019-11-08 | Methods for isolating and expanding cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021008166A2 true BR112021008166A2 (pt) | 2021-08-03 |
Family
ID=64739360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021008166-6A BR112021008166A2 (pt) | 2018-11-08 | 2019-11-08 | métodos para isolamento e expansão de células |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US12460180B2 (https=) |
| EP (1) | EP3877510A1 (https=) |
| JP (2) | JP7665513B2 (https=) |
| KR (1) | KR20210111747A (https=) |
| CN (1) | CN113423821A (https=) |
| AU (1) | AU2019374489B2 (https=) |
| BR (1) | BR112021008166A2 (https=) |
| CA (1) | CA3117913A1 (https=) |
| EA (1) | EA202190864A1 (https=) |
| GB (1) | GB201818243D0 (https=) |
| IL (1) | IL282901A (https=) |
| MX (1) | MX2021005480A (https=) |
| PH (1) | PH12021551038A1 (https=) |
| WO (1) | WO2020095059A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201818243D0 (en) | 2018-11-08 | 2018-12-26 | Gammadelta Therapeutics Ltd | Methods for isolating and expanding cells |
| JP2022545196A (ja) | 2019-08-16 | 2022-10-26 | ガンマデルタ セラピューティクス リミテッド | 抗ｔｃｒデルタ可変１抗体の治療的使用 |
| JP7764364B2 (ja) | 2019-08-16 | 2025-11-05 | ガンマデルタ セラピューティクス リミティッド | エクスビボγδ Ｔ細胞集団 |
| GB202002581D0 (en) | 2020-02-24 | 2020-04-08 | Gammadelta Therapeutics Ltd | Novel antibodies |
| PL4269444T3 (pl) | 2020-08-14 | 2025-01-13 | GammaDelta Therapeutics Limited | Wieloswoiste przeciwciała anty-tcr zmienne delta 1 |
| AU2022224391A1 (en) | 2021-02-17 | 2023-08-24 | Gammadelta Therapeutics Ltd | Anti-tcr delta variable 1 antibodies |
| GB202105113D0 (en) | 2021-04-09 | 2021-05-26 | Gammadelta Therapeutics Ltd | Novel method |
| CA3228168A1 (en) | 2021-08-03 | 2023-02-09 | Gammadelta Therapeutics Ltd | Engineering of gamma delta t cells and compositions thereof |
| EP4504247A1 (en) | 2022-04-04 | 2025-02-12 | Gammadelta Therapeutics Ltd | Novel gene armoring |
| GB202204926D0 (en) | 2022-04-04 | 2022-05-18 | Gammadelta Therapeutics Ltd | Method for expanding gammadelta T cells |
| CN121532496A (zh) * | 2023-07-20 | 2026-02-13 | 武田药品工业株式会社 | 用于制备γδT细胞的无网格方法 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6028176A (en) | 1996-07-19 | 2000-02-22 | Bayer Corporation | High-affinity interleukin-4 muteins |
| US7078034B2 (en) | 1999-01-28 | 2006-07-18 | Palmetto Health Alliance | In vitro activated γ δ lymphocytes |
| CN101120083B (zh) | 2003-10-08 | 2013-03-27 | 威尔森沃尔夫制造公司 | 利用透气性材料进行细胞培养的方法及装置 |
| EP1777294A1 (en) | 2005-10-20 | 2007-04-25 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | IL-15Ralpha sushi domain as a selective and potent enhancer of IL-15 action through IL-15Rbeta/gamma, and hyperagonist (IL15Ralpha sushi -IL15) fusion proteins |
| WO2007111714A2 (en) | 2005-11-28 | 2007-10-04 | Zymogenetics, Inc. | Il-21 antagonists |
| JP4659785B2 (ja) * | 2007-06-15 | 2011-03-30 | トヨタ自動車株式会社 | 内燃機関の燃料噴射制御装置 |
| US8529883B2 (en) | 2010-05-07 | 2013-09-10 | Fibrocell Technologies, Inc. | Dosage unit formulations of autologous dermal fibroblasts |
| CA2860170C (en) | 2010-12-22 | 2022-06-14 | The Board Of Trustees Of The Leland Stanford Junior University | Superagonists and antagonists of interleukin-2 |
| PT2710123T (pt) | 2011-05-19 | 2017-10-13 | Inst De Medicina Molecular | Linha celular de linfócitos compreendendo células t gama-delta, método de composição e produção resultante dos mesmos |
| EP3060059A4 (en) | 2013-10-25 | 2017-11-01 | Board of Regents, The University of Texas System | Polyclonal gamma delta t cells for immunotherapy |
| RU2739770C2 (ru) | 2014-06-11 | 2020-12-28 | полибайосепт ГмбХ | Экспансия лимфоцитов с использованием композиции цитокинов для активной клеточной иммунотерапии |
| IL297773B2 (en) | 2014-11-17 | 2024-07-01 | Adicet Bio Inc | Engineered gamma delta t-cells |
| AU2016274633B2 (en) | 2015-06-09 | 2022-04-21 | Gammadelta Therapeutics Ltd | Methods for the production of TCR gamma delta+ T cells |
| WO2017072367A1 (en) * | 2015-10-30 | 2017-05-04 | Cancer Research Technology Limited | EXPANSION OF NON-HAEMATOPOIETIC TISSUE-RESIDENT γδ T CELLS AND USES OF THESE CELLS |
| KR102618948B1 (ko) | 2016-11-17 | 2023-12-27 | 이오반스 바이오테라퓨틱스, 인크. | 잔유 종양 침윤 림프구 및 그의 제조 및 사용 방법 |
| MX2019007963A (es) | 2017-01-06 | 2019-10-21 | Iovance Biotherapeutics Inc | Expansion de linfocitos infiltrantes de tumor (til) con agonistas de la superfamilia de recptor de factor de necrosis tumoral (tnfrsf) y combinaciones terapeuticas de til- y agonistas de tnfrsf. |
| GB201707048D0 (en) | 2017-05-03 | 2017-06-14 | King S College London | Expansion of gamma delta cells, compositions, and methods of use thereof |
| WO2018229163A1 (en) | 2017-06-14 | 2018-12-20 | King's College London | Methods of activating v delta 2 negative gamma delta t cells |
| GB201818243D0 (en) | 2018-11-08 | 2018-12-26 | Gammadelta Therapeutics Ltd | Methods for isolating and expanding cells |
| CN113423820A (zh) | 2018-11-08 | 2021-09-21 | 伽马三角洲疗法有限公司 | 分离和扩增细胞的方法 |
-
2018
- 2018-11-08 GB GBGB1818243.6A patent/GB201818243D0/en not_active Ceased
-
2019
- 2019-11-08 AU AU2019374489A patent/AU2019374489B2/en active Active
- 2019-11-08 CA CA3117913A patent/CA3117913A1/en active Pending
- 2019-11-08 KR KR1020217013912A patent/KR20210111747A/ko not_active Ceased
- 2019-11-08 BR BR112021008166-6A patent/BR112021008166A2/pt unknown
- 2019-11-08 EP EP19804793.8A patent/EP3877510A1/en active Pending
- 2019-11-08 US US17/292,411 patent/US12460180B2/en active Active
- 2019-11-08 JP JP2021524452A patent/JP7665513B2/ja active Active
- 2019-11-08 WO PCT/GB2019/053166 patent/WO2020095059A1/en not_active Ceased
- 2019-11-08 EA EA202190864A patent/EA202190864A1/ru unknown
- 2019-11-08 MX MX2021005480A patent/MX2021005480A/es unknown
- 2019-11-08 CN CN201980088258.2A patent/CN113423821A/zh active Pending
-
2021
- 2021-05-03 IL IL282901A patent/IL282901A/en unknown
- 2021-05-05 PH PH12021551038A patent/PH12021551038A1/en unknown
-
2025
- 2025-04-09 JP JP2025064036A patent/JP2025106453A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20220010276A1 (en) | 2022-01-13 |
| WO2020095059A1 (en) | 2020-05-14 |
| MX2021005480A (es) | 2021-11-04 |
| CA3117913A1 (en) | 2020-05-14 |
| US12460180B2 (en) | 2025-11-04 |
| EP3877510A1 (en) | 2021-09-15 |
| EA202190864A1 (ru) | 2021-09-14 |
| IL282901A (en) | 2021-06-30 |
| KR20210111747A (ko) | 2021-09-13 |
| JP2025106453A (ja) | 2025-07-15 |
| PH12021551038A1 (en) | 2022-01-03 |
| AU2019374489B2 (en) | 2026-04-02 |
| JP2022506841A (ja) | 2022-01-17 |
| CN113423821A (zh) | 2021-09-21 |
| JP7665513B2 (ja) | 2025-04-21 |
| GB201818243D0 (en) | 2018-12-26 |
| AU2019374489A1 (en) | 2021-05-27 |
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