JP7642813B2 - 生体細胞へのメカノポレーションベースのペイロード送達のための方法及びシステム - Google Patents

生体細胞へのメカノポレーションベースのペイロード送達のための方法及びシステム Download PDF

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JP7642813B2
JP7642813B2 JP2023529914A JP2023529914A JP7642813B2 JP 7642813 B2 JP7642813 B2 JP 7642813B2 JP 2023529914 A JP2023529914 A JP 2023529914A JP 2023529914 A JP2023529914 A JP 2023529914A JP 7642813 B2 JP7642813 B2 JP 7642813B2
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cell
component
processing device
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JP2023550090A (ja
JP2023550090A5 (enExample
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ハン、セウーン
サイチャー、イアン
アレクセーエフ、アレクサンダー
キム、オクチョル
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Cellfe Inc
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Cellfe Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2023529914A 2020-11-18 2021-11-18 生体細胞へのメカノポレーションベースのペイロード送達のための方法及びシステム Active JP7642813B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063115507P 2020-11-18 2020-11-18
US63/115,507 2020-11-18
PCT/US2021/059856 WO2022109113A1 (en) 2020-11-18 2021-11-18 Methods and systems for mechanoporation-based payload delivery into biological cells

Publications (3)

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JP2023550090A JP2023550090A (ja) 2023-11-30
JP2023550090A5 JP2023550090A5 (enExample) 2024-11-14
JP7642813B2 true JP7642813B2 (ja) 2025-03-10

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US (2) US11788050B2 (enExample)
EP (1) EP4232554A1 (enExample)
JP (1) JP7642813B2 (enExample)
KR (1) KR102819986B1 (enExample)
CN (1) CN117015596B (enExample)
AU (1) AU2021383756A1 (enExample)
CA (1) CA3201944A1 (enExample)
IL (1) IL302976A (enExample)
WO (1) WO2022109113A1 (enExample)

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* Cited by examiner, † Cited by third party
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CA3201944A1 (en) 2020-11-18 2022-05-27 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
CN115254214B (zh) * 2022-06-29 2023-07-25 中国科学院精密测量科学与技术创新研究院 一种微流控通道、微流控芯片和生化分子递送方法
US20250290023A1 (en) * 2024-03-15 2025-09-18 Cellfe, Inc. High-Throughput Mechanoporation for Scalable and Cost-Effective Cell Therapy Manufacturing

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WO2020210162A1 (en) 2019-04-08 2020-10-15 Sqz Biotechnologies Company Cartridge for use in a system for delivery of a payload into a cell

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WO2022109113A1 (en) 2022-05-27
CA3201944A1 (en) 2022-05-27
US20230416781A1 (en) 2023-12-28
JP2023550090A (ja) 2023-11-30
CN117015596A (zh) 2023-11-07
AU2021383756A1 (en) 2023-06-22
IL302976A (en) 2023-07-01
KR20230110308A (ko) 2023-07-21
US20230174918A1 (en) 2023-06-08
KR102819986B1 (ko) 2025-06-12
EP4232554A1 (en) 2023-08-30
US11788050B2 (en) 2023-10-17
US12359158B2 (en) 2025-07-15
CN117015596B (zh) 2024-02-09

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