KR102819986B1 - 생물학적 세포로 기계적 천공 기반 페이로드 전달을 위한 방법 및 시스템 - Google Patents

생물학적 세포로 기계적 천공 기반 페이로드 전달을 위한 방법 및 시스템 Download PDF

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KR102819986B1
KR102819986B1 KR1020237020303A KR20237020303A KR102819986B1 KR 102819986 B1 KR102819986 B1 KR 102819986B1 KR 1020237020303 A KR1020237020303 A KR 1020237020303A KR 20237020303 A KR20237020303 A KR 20237020303A KR 102819986 B1 KR102819986 B1 KR 102819986B1
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KR20230110308A (ko
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세운 한
이안 시커
알렉산더 알렉시브
옥철 김
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셀에프이, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020237020303A 2020-11-18 2021-11-18 생물학적 세포로 기계적 천공 기반 페이로드 전달을 위한 방법 및 시스템 Active KR102819986B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063115507P 2020-11-18 2020-11-18
US63/115,507 2020-11-18
PCT/US2021/059856 WO2022109113A1 (en) 2020-11-18 2021-11-18 Methods and systems for mechanoporation-based payload delivery into biological cells

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KR20230110308A KR20230110308A (ko) 2023-07-21
KR102819986B1 true KR102819986B1 (ko) 2025-06-12

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US (2) US11788050B2 (enExample)
EP (1) EP4232554A1 (enExample)
JP (1) JP7642813B2 (enExample)
KR (1) KR102819986B1 (enExample)
CN (1) CN117015596B (enExample)
AU (1) AU2021383756A1 (enExample)
CA (1) CA3201944A1 (enExample)
IL (1) IL302976A (enExample)
WO (1) WO2022109113A1 (enExample)

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CA3201944A1 (en) 2020-11-18 2022-05-27 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
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US20250290023A1 (en) * 2024-03-15 2025-09-18 Cellfe, Inc. High-Throughput Mechanoporation for Scalable and Cost-Effective Cell Therapy Manufacturing

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WO2020037117A1 (en) 2018-08-15 2020-02-20 Georgia Tech Research Corporation Analysis system and methods of use thereof

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Publication number Publication date
WO2022109113A1 (en) 2022-05-27
CA3201944A1 (en) 2022-05-27
US20230416781A1 (en) 2023-12-28
JP7642813B2 (ja) 2025-03-10
JP2023550090A (ja) 2023-11-30
CN117015596A (zh) 2023-11-07
AU2021383756A1 (en) 2023-06-22
IL302976A (en) 2023-07-01
KR20230110308A (ko) 2023-07-21
US20230174918A1 (en) 2023-06-08
EP4232554A1 (en) 2023-08-30
US11788050B2 (en) 2023-10-17
US12359158B2 (en) 2025-07-15
CN117015596B (zh) 2024-02-09

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