Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
  • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
  • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
  • C12N15/09—Recombinant DNA-technology
  • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
  • C12N5/0062—General methods for three-dimensional culture
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Definitions

• FIG. 4B is a schematic exploded view of the cell processing apparatus in FIG. 4A.
• Microfluidic techniques provide new opportunities for processing and manipulation of biological cells, such as the delivery of payload into cells for gene engineering and other applications.
• a “microfluidic” technique is defined as a process of passing fluid through a channel, which has the smallest dimension of less than 1 millimeter.
• an apparatus may include one or more constrictions forming a gap that is less than 1 millimeter.
• microfluidic techniques are the small size of flow channels, resulting in flow conditions represented by low Reynolds numbers (e.g., less than 1). As such, fluid viscosity is a dominant factor of these flow conditions. Furthermore, the overall throughput of a single channel is limited. For example, a volumetric flow rate of a single channel having a width of 1 millimeter can be less than 1 ml/min. This flow rate corresponds to the processing speed of about 10 7 cells per hour. At the same time, industrial applications require much larger processing speeds, such as 10 9 or 10 10 cells per hour, which has significantly limited the adoption of microfluidic techniques in the past.
• FIG. 1A and FIG. IB are schematic representations of cell processing apparatus 100 (in an assembled form and exploded view), in accordance with some examples.
• Cell processing apparatus 100 comprises processing assembly 110, inlet component 102, and stopper 104.
• Processing assembly 110 is disposed between inlet component 102 and stopper 104. More specifically, each inlet component 102 and stopper 104 is sealed against processing assembly 110.
• processing assembly 110, inlet component 102, and stopper 104 define and enclose cavity 107 of cell processing apparatus 100. Cavity 107 can be also referred to as an inlet opening.
• cell processing apparatus 100 also comprises distribution component 106, positioned inside cavity 107.
• ridges 140 are clustered closer to the channel inlet, e.g., to allow longer time for cells to remain in the fluid with higher speed after interactions with ridges 140.
• the channel inlet and/or the channel outlet are located at the front and back edges of processing components 119.
• the channel inlet and/or outlet can be arranged through channel wall 132 of processing component 119, e.g., to reduce the amount of dust entering each channel 130 due to the fabrication process.

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• Engineering & Computer Science (AREA)
• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Genetics & Genomics (AREA)
• Zoology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Organic Chemistry (AREA)
• Wood Science & Technology (AREA)
• Chemical & Material Sciences (AREA)
• Biotechnology (AREA)
• Biomedical Technology (AREA)
• General Engineering & Computer Science (AREA)
• Microbiology (AREA)
• General Health & Medical Sciences (AREA)
• Biochemistry (AREA)
• Sustainable Development (AREA)
• Cell Biology (AREA)
• Mechanical Engineering (AREA)
• Physics & Mathematics (AREA)
• Biophysics (AREA)
• Molecular Biology (AREA)
• Plant Pathology (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)
• Micro-Organisms Or Cultivation Processes Thereof (AREA)
• Medicines Containing Material From Animals Or Micro-Organisms (AREA)

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Cited By (4)

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• 2021
  • 2021-11-18 CA CA3201944A patent/CA3201944A1/en active Pending
  • 2021-11-18 CN CN202180090664.XA patent/CN117015596B/zh active Active
  • 2021-11-18 AU AU2021383756A patent/AU2021383756A1/en not_active Abandoned
  • 2021-11-18 WO PCT/US2021/059856 patent/WO2022109113A1/en not_active Ceased
  • 2021-11-18 KR KR1020237020303A patent/KR102819986B1/ko active Active
  • 2021-11-18 JP JP2023529914A patent/JP7642813B2/ja active Active
  • 2021-11-18 IL IL302976A patent/IL302976A/en unknown
  • 2021-11-18 EP EP21895569.8A patent/EP4232554A1/en active Pending
• 2023
  • 2023-01-31 US US18/162,372 patent/US11788050B2/en active Active
  • 2023-08-25 US US18/456,340 patent/US12359158B2/en active Active

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