JP7489377B2 - 多能性幹細胞を含む細胞集団及びその製造方法 - Google Patents

多能性幹細胞を含む細胞集団及びその製造方法 Download PDF

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JP7489377B2
JP7489377B2 JP2021511858A JP2021511858A JP7489377B2 JP 7489377 B2 JP7489377 B2 JP 7489377B2 JP 2021511858 A JP2021511858 A JP 2021511858A JP 2021511858 A JP2021511858 A JP 2021511858A JP 7489377 B2 JP7489377 B2 JP 7489377B2
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一博 竹内
将人 伊吹
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Kaneka Corp
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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JP2021511858A 2019-03-29 2020-03-25 多能性幹細胞を含む細胞集団及びその製造方法 Active JP7489377B2 (ja)

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JP2019066845 2019-03-29
JP2019066845 2019-03-29
PCT/JP2020/013279 WO2020203538A1 (ja) 2019-03-29 2020-03-25 多能性幹細胞を含む細胞集団及びその製造方法

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JP7489377B2 true JP7489377B2 (ja) 2024-05-23

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Publication number Priority date Publication date Assignee Title
EP3587560A4 (en) * 2017-01-27 2020-12-16 Kaneka Corporation Endodermal cell mass, and method for producing any one of three primary germ layer cell mass from pluripotent cells
WO2023017806A1 (ja) * 2021-08-10 2023-02-16 株式会社カネカ 多能性幹細胞の製造方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016052759A1 (ja) 2014-10-02 2016-04-07 学校法人自治医科大学 多能性幹細胞再樹立法
WO2017175876A1 (ja) 2016-04-05 2017-10-12 学校法人自治医科大学 幹細胞を再樹立する方法
WO2018164240A1 (ja) 2017-03-08 2018-09-13 大日本住友製薬株式会社 網膜色素上皮細胞の製造方法
WO2018235583A1 (ja) 2017-06-19 2018-12-27 公益財団法人神戸医療産業都市推進機構 多能性幹細胞の分化能の予測方法及びそのための試薬

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030125344A1 (en) 2001-03-23 2003-07-03 Bayer Corporation Rho-kinase inhibitors
MXPA03008659A (es) 2001-03-23 2005-04-08 Bayer Ag Inhibidores de rho-cinasa.
DE60320933D1 (de) 2002-01-10 2008-06-26 Bayer Healthcare Ag Rho-kinase inhibitoren
JP4469179B2 (ja) 2002-01-23 2010-05-26 バイエル ファーマセチカル コーポレーション Rhoキナーゼ阻害剤としてのピリミジン誘導体
MXPA04007196A (es) 2002-01-23 2005-06-08 Bayer Pharmaceuticals Corp Inhibidores de rho-quinasa.
ES2273047T3 (es) 2002-10-28 2007-05-01 Bayer Healthcare Ag Fenilaminopirimidinas sustituidas con heteroariloxi como inhibidores de rho-cinasa.
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
US20070212335A1 (en) * 2006-03-07 2007-09-13 Hantash Basil M Treatment of alopecia by micropore delivery of stem cells
WO2012011610A1 (en) 2010-07-21 2012-01-26 Kyoto University Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell
SG187655A1 (en) 2010-08-09 2013-03-28 Takeda Pharmaceutical Method of producing pancreatic hormone-producing cells
WO2016060260A1 (ja) 2014-10-16 2016-04-21 国立大学法人京都大学 組織片
JP6682446B2 (ja) 2014-10-24 2020-04-15 大日本住友製薬株式会社 網膜組織の製造方法
WO2017023971A1 (en) * 2015-08-03 2017-02-09 Oncomed Pharmaceuticals, Inc. Identification of predictive biomarkers associated with wnt pathway inhibitors
CA2994192C (en) * 2015-08-13 2020-06-16 Peking University Induced extended pluripotent stem cells, methods of making and using
US10007128B1 (en) 2017-10-02 2018-06-26 Carl Zeiss Vision International Gmbh Method and device for establishing a target design

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016052759A1 (ja) 2014-10-02 2016-04-07 学校法人自治医科大学 多能性幹細胞再樹立法
WO2017175876A1 (ja) 2016-04-05 2017-10-12 学校法人自治医科大学 幹細胞を再樹立する方法
WO2018164240A1 (ja) 2017-03-08 2018-09-13 大日本住友製薬株式会社 網膜色素上皮細胞の製造方法
WO2018235583A1 (ja) 2017-06-19 2018-12-27 公益財団法人神戸医療産業都市推進機構 多能性幹細胞の分化能の予測方法及びそのための試薬

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NAKAGAWA M. et al.,A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells,Sci Rep.,2014年01月08日,4:3594,DOI:10.1038/srep03594
TANO K. et al.,A Novel In Vitro Method for Detecting Undifferentiated Human Pluripotent Stem Cells as Impurities in Cell Therapy Products Using a Highly Efficient Culture System,PLOS ONE,2014年10月27日,Vol.9, Issue 10,e110496,DOI: 10.1371/journal.pone.0110496

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EP3950933A1 (en) 2022-02-09
EP3950933A4 (en) 2023-01-11
US20220169977A1 (en) 2022-06-02
JPWO2020203538A1 (https=) 2020-10-08

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