JP7458680B2 - 新規加水分解酵素及びそれを利用した(1s,2s)-1-アルコキシカルボニル-2-ビニルシクロプロパンカルボン酸の製造方法 - Google Patents

新規加水分解酵素及びそれを利用した(1s,2s)-1-アルコキシカルボニル-2-ビニルシクロプロパンカルボン酸の製造方法 Download PDF

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JP7458680B2
JP7458680B2 JP2020511068A JP2020511068A JP7458680B2 JP 7458680 B2 JP7458680 B2 JP 7458680B2 JP 2020511068 A JP2020511068 A JP 2020511068A JP 2020511068 A JP2020511068 A JP 2020511068A JP 7458680 B2 JP7458680 B2 JP 7458680B2
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amino acid
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alkoxycarbonyl
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豊和 吉田
浩一 石田
良磨 三宅
崇敦 井浦
潤 川端
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API Corp
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    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
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    • C12P41/003Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions
    • C12P41/005Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions by esterification of carboxylic acid groups in the enantiomers or the inverse reaction
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    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/62Carboxylic acid esters
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    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)

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JP2020511068A 2018-03-30 2019-03-29 新規加水分解酵素及びそれを利用した(1s,2s)-1-アルコキシカルボニル-2-ビニルシクロプロパンカルボン酸の製造方法 Active JP7458680B2 (ja)

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JP2018070188 2018-03-30
JP2018070188 2018-03-30
PCT/JP2019/013926 WO2019189724A1 (ja) 2018-03-30 2019-03-29 新規加水分解酵素及びそれを利用した(1s,2s)-1-アルコキシカルボニル-2-ビニルシクロプロパンカルボン酸の製造方法

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CN110283804A (zh) * 2019-07-18 2019-09-27 江苏省农业科学院 酯酶基因及编码蛋白与应用
CN110643625A (zh) * 2019-09-23 2020-01-03 上海应用技术大学 一种重组表达质粒与基因工程菌及(4s,5r)-半酯的制备方法

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2011102388A1 (ja) 2010-02-16 2011-08-25 株式会社エーピーアイ コーポレーション 1-アミノ-1-アルコキシカルボニル-2-ビニルシクロプロパンの製造方法
WO2012029819A1 (ja) 2010-08-31 2012-03-08 株式会社エーピーアイ コーポレーション 新規加水分解酵素タンパク質
WO2013094499A1 (ja) 2011-12-19 2013-06-27 住友化学株式会社 光学活性なα-置換-β-アミノ酸の製造方法

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
CS199542B2 (en) 1970-07-13 1980-07-31 Giacomo Cevales Process for thermal treatment of refractory material prepared by the heat for increasing corrosion resistance
JPS57183799A (en) 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
DE60323215D1 (de) * 2002-03-19 2008-10-09 Mitsubishi Chem Corp Neue carbonylreduktase, diese codierendes gen und verfahren zur produktion optisch-aktiver alkohole damit
CA3007908A1 (en) 2003-03-07 2005-04-14 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
JP2005034025A (ja) 2003-07-18 2005-02-10 Mitsubishi Pharma Corp 大腸菌での異種遺伝子発現を促進させるプラスミドベクター、組換え大腸菌、該組換え大腸菌を用いた化学物質製造法及び組換えタンパク質の製造法
US20140142337A1 (en) * 2011-06-21 2014-05-22 Mitsubishi Gas Chemical Company, Inc. 1-amino-2-vinylcyclopropane carboxylic acid amide and salt thereof, and method for producing same
JP2018070188A (ja) 2016-10-25 2018-05-10 キヤノン株式会社 緩衝部材、梱包容器、及び画像形成装置用の梱包容器

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011102388A1 (ja) 2010-02-16 2011-08-25 株式会社エーピーアイ コーポレーション 1-アミノ-1-アルコキシカルボニル-2-ビニルシクロプロパンの製造方法
WO2012029819A1 (ja) 2010-08-31 2012-03-08 株式会社エーピーアイ コーポレーション 新規加水分解酵素タンパク質
WO2013094499A1 (ja) 2011-12-19 2013-06-27 住友化学株式会社 光学活性なα-置換-β-アミノ酸の製造方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
" alpha/beta hydrolase [Microbacterium chocolatum]",WP_053548180,[online], National Center for Biotechnology Inform,https://www.ncbi.nlm.nih.gov/protein/924869447?sat=47&satkey=7881781
" alpha/beta hydrolase [Pseudonocardia dioxanivorans]",WP_013673631,[online], National Center for Biotechnology Inform,https://www.ncbi.nlm.nih.gov/protein/WP_013673631
" Alpha/beta hydrolase {ECO:0000313|EMBL:OZC62876.1}",A0A259Y6X7, [online], UniProtKB,https://www.uniprot.org/uniprot/A0A259Y6X7.txt?version=3

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US11572551B2 (en) 2023-02-07
CN111918969B (zh) 2024-11-19
EP3778898A1 (en) 2021-02-17
US20230323317A1 (en) 2023-10-12
JPWO2019189724A1 (ja) 2021-04-01
CN111918969A (zh) 2020-11-10
US12258593B2 (en) 2025-03-25
WO2019189724A1 (ja) 2019-10-03
US20210024905A1 (en) 2021-01-28
EP3778898A4 (en) 2022-02-09

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