JP7427180B2 - 毛包原基及びその製造方法 - Google Patents

毛包原基及びその製造方法 Download PDF

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JP7427180B2
JP7427180B2 JP2021518296A JP2021518296A JP7427180B2 JP 7427180 B2 JP7427180 B2 JP 7427180B2 JP 2021518296 A JP2021518296 A JP 2021518296A JP 2021518296 A JP2021518296 A JP 2021518296A JP 7427180 B2 JP7427180 B2 JP 7427180B2
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culture
cells
epithelial cells
mesenchymal cells
hair follicle
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JPWO2020225934A1 (https=
JPWO2020225934A5 (https=
Inventor
淳二 福田
達斗 景山
亮啓 清水
陸満 中嶋
理樹 穴竃
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Yokohama National University NUC
Kanagawa Institute of Industrial Science and Technology
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Yokohama National University NUC
Kanagawa Institute of Industrial Science and Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C12N5/0628—Hair stem cells; Hair progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/092—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1352—Mesenchymal stem cells
    • C12N2502/1388—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Dermatology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2021518296A 2019-05-07 2019-08-07 毛包原基及びその製造方法 Active JP7427180B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019087747 2019-05-07
JP2019087747 2019-05-07
PCT/JP2019/031142 WO2020225934A1 (ja) 2019-05-07 2019-08-07 毛包原基及びその製造方法

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JPWO2020225934A1 JPWO2020225934A1 (https=) 2020-11-12
JPWO2020225934A5 JPWO2020225934A5 (https=) 2022-07-21
JP7427180B2 true JP7427180B2 (ja) 2024-02-05

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US (1) US20220228113A1 (https=)
EP (1) EP3967749A4 (https=)
JP (1) JP7427180B2 (https=)
CN (1) CN113785047B (https=)
WO (1) WO2020225934A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7847810B2 (ja) * 2021-10-08 2026-04-20 国立大学法人横浜国立大学 毛髪再生能を有する細胞凝集塊の製造方法及びこれに関連する方法
JP2023056592A (ja) * 2021-10-08 2023-04-20 国立大学法人横浜国立大学 移植用毛様組織及びこれに関連する方法
JP2025067221A (ja) * 2023-10-12 2025-04-24 国立大学法人横浜国立大学 毛乳頭細胞活性化用組成物

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018082638A (ja) 2016-11-21 2018-05-31 国立大学法人横浜国立大学 毛細血管構造を有する再生毛包原基の集合体の製造方法、毛包組織含有シート、及び毛包組織含有シートの製造方法
WO2019039376A1 (ja) 2017-08-22 2019-02-28 国立大学法人横浜国立大学 複数の再生毛包原基の製造方法、毛包組織含有シートの製造方法、毛髪再生用キット及び発毛促進又は抑制物質をスクリーニングする方法

Family Cites Families (6)

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Publication number Priority date Publication date Assignee Title
GB0605450D0 (en) * 2006-03-17 2006-04-26 Intercytex Ltd Cell co-culture
EP2105499A1 (en) * 2008-03-28 2009-09-30 Technische Universität Berlin Methods for producing de novo papillae and hair microfollicles and their use for in vitro tests and in vivo implantations
EP2991692B1 (en) * 2013-05-03 2019-08-21 The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. Skin substitutes and methods for hair follicle neogenesis
US20180355315A1 (en) 2014-09-08 2018-12-13 Organ Technologies, Inc. Method of producing full thickness skin having skin accessory organs
EP3351621B1 (en) 2015-10-30 2023-11-22 National University Corporation Yokohama National University Regenerated hair follicle primordium aggregation manufacturing method, hair follicle tissue-containing sheet, and method for manufacturing hair follicle tissue-containing sheet
CN109395165A (zh) * 2018-12-21 2019-03-01 江阴司特易生物技术有限公司 一种人工复合神经导管及制备方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018082638A (ja) 2016-11-21 2018-05-31 国立大学法人横浜国立大学 毛細血管構造を有する再生毛包原基の集合体の製造方法、毛包組織含有シート、及び毛包組織含有シートの製造方法
WO2019039376A1 (ja) 2017-08-22 2019-02-28 国立大学法人横浜国立大学 複数の再生毛包原基の製造方法、毛包組織含有シートの製造方法、毛髪再生用キット及び発毛促進又は抑制物質をスクリーニングする方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Cell Reports，2018年，vol.22，p.242-254
Corningマトリゲル基底膜マトリックス，コーニングインターナショナル株式会社ライフサイエン，2016年，p.1-31，p.3

Also Published As

Publication number Publication date
JPWO2020225934A1 (https=) 2020-11-12
EP3967749A4 (en) 2023-05-03
CN113785047B (zh) 2025-08-08
EP3967749A1 (en) 2022-03-16
WO2020225934A1 (ja) 2020-11-12
CN113785047A (zh) 2021-12-10
US20220228113A1 (en) 2022-07-21

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