JP7318166B2 - マイクロｒｎａ２２の阻害剤 - Google Patents
マイクロｒｎａ２２の阻害剤 Download PDFInfo
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- JP7318166B2 JP7318166B2 JP2020573079A JP2020573079A JP7318166B2 JP 7318166 B2 JP7318166 B2 JP 7318166B2 JP 2020573079 A JP2020573079 A JP 2020573079A JP 2020573079 A JP2020573079 A JP 2020573079A JP 7318166 B2 JP7318166 B2 JP 7318166B2
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- A61P3/06—Antihyperlipidemics
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- C12N2310/11—Antisense
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| US201862642934P | 2018-03-14 | 2018-03-14 | |
| US62/642,934 | 2018-03-14 | ||
| PCT/US2019/022351 WO2019178411A1 (en) | 2018-03-14 | 2019-03-14 | Inhibitors of micro-rna 22 |
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| JP2021518159A JP2021518159A (ja) | 2021-08-02 |
| JP2021518159A5 JP2021518159A5 (https=) | 2022-03-16 |
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| KR20200131287A (ko) | 2018-03-14 | 2020-11-23 | 베스 이스라엘 데코니스 메디칼 센터 | 마이크로-rna 22의 억제제 |
| CN110609142A (zh) * | 2019-10-09 | 2019-12-24 | 广州医科大学附属第二医院 | 一种检测外周血fto蛋白与自身调控元件结合能力的方法与应用 |
| CN115029347B (zh) * | 2022-05-11 | 2024-02-20 | 珠海中科先进技术研究院有限公司 | 识别和调控肝肾细胞纤维化的分子监测序列、重组质粒、抑制病毒 |
| CN116082447A (zh) * | 2022-09-09 | 2023-05-09 | 湖南大学 | 一种多肽及其制备方法 |
| CN117304258B (zh) * | 2022-09-09 | 2024-09-24 | 湖南大学 | 一种多肽的用途 |
| EP4353823A1 (en) * | 2022-10-12 | 2024-04-17 | Resalis Therapeutics S.r.l. | Inhibitors of micro-rna 22 |
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| US5981505A (en) | 1993-01-26 | 1999-11-09 | The Trustees Of The University Of Pennsylvania | Compositions and methods for delivery of genetic material |
| US5837533A (en) | 1994-09-28 | 1998-11-17 | American Home Products Corporation | Complexes comprising a nucleic acid bound to a cationic polyamine having an endosome disruption agent |
| US5840710A (en) | 1994-12-09 | 1998-11-24 | Genzyme Corporation | Cationic amphiphiles containing ester or ether-linked lipophilic groups for intracellular delivery of therapeutic molecules |
| US6217900B1 (en) | 1997-04-30 | 2001-04-17 | American Home Products Corporation | Vesicular complexes and methods of making and using the same |
| CA2294988C (en) | 1997-07-01 | 2015-11-24 | Isis Pharmaceuticals Inc. | Compositions and methods for the delivery of oligonucleotides via the alimentary canal |
| JP2002516256A (ja) | 1998-05-26 | 2002-06-04 | アイ・シー・エヌ・フアーマシユーテイカルズ・インコーポレイテツド | 二環式糖成分を有する新規ヌクレオシド |
| US6838283B2 (en) | 1998-09-29 | 2005-01-04 | Isis Pharmaceuticals Inc. | Antisense modulation of survivin expression |
| US6693187B1 (en) | 2000-10-17 | 2004-02-17 | Lievre Cornu Llc | Phosphinoamidite carboxlates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge |
| CA2487274A1 (en) | 2002-05-06 | 2003-11-13 | Nucleonics Inc. | Spermine chemically linked to lipids and cell-specific targeting molecules as a transfection agent |
| EP2302055B1 (en) * | 2004-11-12 | 2014-08-27 | Asuragen, Inc. | Methods and compositions involving miRNA and miRNA inhibitor molecules |
| US20070213292A1 (en) * | 2005-08-10 | 2007-09-13 | The Rockefeller University | Chemically modified oligonucleotides for use in modulating micro RNA and uses thereof |
| KR101485071B1 (ko) * | 2005-12-12 | 2015-01-26 | 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 | 마이크로 ｒｎａ를 이용한 근육 세포 증식 및 분화 조절방법과 근육 세포 치료방법 및 유전자 발현 억제방법그리고 마이크로 ｒｎａ 분자의 발현용 벡터와 이를 이용한키트 및 미오사이트 |
| AU2013254923A1 (en) * | 2006-04-03 | 2013-11-28 | Santaris Pharma A/S | Pharmaceutical compositions comprising anti-miRNA antisense oligonucleotide |
| EP2623599B1 (en) * | 2007-10-04 | 2019-01-02 | Roche Innovation Center Copenhagen A/S | Micromirs |
| EP2419537B1 (en) * | 2009-04-13 | 2014-01-29 | Somagenics, Inc. | Methods and compositions for detection of small rnas |
| SG176716A1 (en) * | 2009-06-08 | 2012-01-30 | Miragen Therapeutics | CHEMICAL MODIFICATION MOTIFS FOR miRNA INHIBITORS AND MIMETICS |
| UY33807A (es) * | 2010-12-17 | 2012-07-31 | Sanofi Sa | miRNAs como indicadores del estado tisular o de enfermedades como osteoartritis |
| CA2832899A1 (en) | 2011-04-12 | 2012-10-18 | Beth Israel Deaconess Medical Center, Inc. | Micro-rna inhibitors and their uses in disease |
| JP6092655B2 (ja) * | 2012-02-23 | 2017-03-08 | 善基 村上 | テスト体液サンプルの分類方法 |
| US9034839B2 (en) | 2012-04-20 | 2015-05-19 | Aptamir Therapeutics, Inc. | miRNA modulators of thermogenesis |
| WO2013181613A1 (en) * | 2012-05-31 | 2013-12-05 | Research Development Foundation | Mirna for the diagnosis and treatment of autoimmune and inflammatory disease |
| CN107236735B (zh) * | 2012-06-21 | 2019-11-08 | 米拉根医疗股份有限公司 | 包含锁核酸基序的基于寡核苷酸的抑制剂 |
| US9822358B2 (en) | 2013-10-18 | 2017-11-21 | Beth Israel Deaconess Medical Center | Treatment of cancers with micro-RNA inhibitors |
| CA2935426C (en) * | 2014-01-30 | 2023-07-25 | F. Hoffmann-La Roche Ag | Polyoligomer compound with biocleavable conjugates for reducing or inhibiting expression of a nucleic acid target |
| WO2016127002A1 (en) | 2015-02-04 | 2016-08-11 | Bristol-Myers Squibb Company | Lna oligonucleotides with alternating flanks |
| WO2016149358A1 (en) * | 2015-03-16 | 2016-09-22 | Duncan Ross | Method of treatment comprising membrane-enclosed vesicle |
| WO2017187426A1 (en) * | 2016-04-29 | 2017-11-02 | Aptamir Therapeutics, Inc. | Inhibition of mir-22 mirna by apt-110 |
| CN107432933A (zh) * | 2017-07-13 | 2017-12-05 | 中南大学湘雅三医院 | miR‑22作为靶位点在制备膀胱癌的化疗增敏治疗药物中的应用 |
| WO2019053235A1 (en) * | 2017-09-15 | 2019-03-21 | Genfit | NON-INVASIVE DIAGNOSIS OF NON ALCOHOLIC LIVER DISEASES, NON ALCOHOLIC STHEATHEPATITIS AND / OR HEPATIC FIBROSIS |
| KR20200131287A (ko) | 2018-03-14 | 2020-11-23 | 베스 이스라엘 데코니스 메디칼 센터 | 마이크로-rna 22의 억제제 |
| WO2019217907A1 (en) * | 2018-05-11 | 2019-11-14 | The Regents Of The University Of California | Methods and compositions for the treatment of hepatic and metabolic diseases |
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