JP7269648B2 - セレノシステインを含む組換えポリペプチド及びその製造方法 - Google Patents

セレノシステインを含む組換えポリペプチド及びその製造方法 Download PDF

Info

Publication number
JP7269648B2
JP7269648B2 JP2019500781A JP2019500781A JP7269648B2 JP 7269648 B2 JP7269648 B2 JP 7269648B2 JP 2019500781 A JP2019500781 A JP 2019500781A JP 2019500781 A JP2019500781 A JP 2019500781A JP 7269648 B2 JP7269648 B2 JP 7269648B2
Authority
JP
Japan
Prior art keywords
seq
position corresponding
substitution
amino acid
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2019500781A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019520083A (ja
JP2019520083A5 (enExample
Inventor
アンドリュー ディー． エリントン，
ロス タイヤー，
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
Original Assignee
University of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System filed Critical University of Texas System
Publication of JP2019520083A publication Critical patent/JP2019520083A/ja
Publication of JP2019520083A5 publication Critical patent/JP2019520083A5/ja
Priority to JP2023029563A priority Critical patent/JP2023060001A/ja
Application granted granted Critical
Publication of JP7269648B2 publication Critical patent/JP7269648B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immunology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Oncology (AREA)
JP2019500781A 2016-07-11 2017-07-10 セレノシステインを含む組換えポリペプチド及びその製造方法 Active JP7269648B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023029563A JP2023060001A (ja) 2016-07-11 2023-02-28 セレノシステインを含む組換えポリペプチド及びその製造方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662360745P 2016-07-11 2016-07-11
US62/360,745 2016-07-11
PCT/US2017/041373 WO2018013481A1 (en) 2016-07-11 2017-07-10 Recombinant polypeptides comprising selenocysteine and method for producing the same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023029563A Division JP2023060001A (ja) 2016-07-11 2023-02-28 セレノシステインを含む組換えポリペプチド及びその製造方法

Publications (3)

Publication Number Publication Date
JP2019520083A JP2019520083A (ja) 2019-07-18
JP2019520083A5 JP2019520083A5 (enExample) 2020-08-20
JP7269648B2 true JP7269648B2 (ja) 2023-05-09

Family

ID=60953318

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2019500781A Active JP7269648B2 (ja) 2016-07-11 2017-07-10 セレノシステインを含む組換えポリペプチド及びその製造方法
JP2023029563A Withdrawn JP2023060001A (ja) 2016-07-11 2023-02-28 セレノシステインを含む組換えポリペプチド及びその製造方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2023029563A Withdrawn JP2023060001A (ja) 2016-07-11 2023-02-28 セレノシステインを含む組換えポリペプチド及びその製造方法

Country Status (6)

Country Link
US (1) US11155804B2 (enExample)
EP (1) EP3481945A4 (enExample)
JP (2) JP7269648B2 (enExample)
CN (1) CN109844105A (enExample)
CA (1) CA3030163A1 (enExample)
WO (1) WO2018013481A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3034701A1 (en) 2016-08-30 2018-03-08 Board Of Regents, The University Of Texas System Production of seleno-biologics in genomically recoded organisms
CN109486802B (zh) * 2018-12-26 2020-08-04 广州白云山拜迪生物医药有限公司 一种酶活提高的青霉素酶突变体及其构建方法
WO2025024577A1 (en) * 2023-07-24 2025-01-30 Board Of Regents, The University Of Texas System Incorporating unnatural amino acids using one pot pure system
CN120158456A (zh) * 2023-10-18 2025-06-17 河南农业大学 玉米ZmmtRF2a基因突变标记、鉴定引物及其在调控籽粒发育中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003029469A1 (en) 2001-09-13 2003-04-10 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Novel selenocystein-containing proteins
JP2010509222A (ja) 2006-11-02 2010-03-25 ダニエル・ジェイ・カポン 可動部を備えたハイブリッド免疫グロブリン

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10309193A (ja) 1997-05-12 1998-11-24 Sankyo Co Ltd 哺乳類セレノシステイン含有タンパク質の大腸菌での製造法
US6849417B1 (en) 1998-04-06 2005-02-01 The United States Of America As Represented By The Department Of Health And Human Services Mammalian selenoprotein differentially expressed in tumor cells
AU6987200A (en) * 1999-08-16 2001-03-13 Karolinska Innovations Ab Methods and means for selenoprotein expression
WO2007075438A2 (en) 2005-12-15 2007-07-05 Codon Devices, Inc. Polypeptides comprising unnatural amino acids, methods for their production and uses therefor
WO2009043051A2 (en) 2007-09-27 2009-04-02 Biogen Idec Ma Inc. Cd23 binding molecules and methods of use thereof
KR101656107B1 (ko) 2007-11-20 2016-09-08 암브룩스, 인코포레이티드 변형된 인슐린 폴리펩티드 및 이의 용도
MX2013008736A (es) 2011-01-28 2013-11-22 Ca Nat Research Council Diseño de dominios de inmunoglobulina.
DK2717898T3 (en) * 2011-06-10 2019-03-25 Bioverativ Therapeutics Inc COAGULATING COMPOUNDS AND PROCEDURES FOR USE THEREOF
US10240158B2 (en) 2011-07-11 2019-03-26 Yale University Compositions and methods for making selenocysteine containing polypeptides
US9464288B2 (en) 2011-07-11 2016-10-11 Yale University Compositions and methods for making selenocysteine containing polypeptides
US10876142B2 (en) 2011-07-11 2020-12-29 Yale University Compositions and methods for making selenocysteine containing polypeptides
EP2968552B1 (en) 2013-03-14 2020-03-11 The Scripps Research Institute Targeting agent antibody conjugates and uses thereof
US20150050682A1 (en) 2013-08-15 2015-02-19 University Of Vermont And State Agricultural College Direct assay of thioredoxin reductase activity
WO2015066543A1 (en) 2013-11-01 2015-05-07 Board Of Regents, The University Of Texas System Targeting her2 and her3 with bispecific antibodies in cancerous cells
US10118950B2 (en) 2014-08-30 2018-11-06 Northwestern University Platforms for cell-free protein synthesis comprising extracts from genomically recoded E. coli strains having genetic knock-out mutations in release factor 1 (RF-1) and endA
WO2016172269A2 (en) 2015-04-20 2016-10-27 University Of Utah Research Foundation Insulin analogs having shortened b chain peptides and associated methods
US10557160B2 (en) 2015-12-15 2020-02-11 Board Of Regents, The University Of Texas System Transgenic bacteria with expanded amino acid usage and nucleic acid molecules for use in the same
AU2017298565B2 (en) 2016-07-22 2021-08-19 The Walter And Eliza Hall Institute Of Medical Research Insulin analogs
CA3034701A1 (en) 2016-08-30 2018-03-08 Board Of Regents, The University Of Texas System Production of seleno-biologics in genomically recoded organisms

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003029469A1 (en) 2001-09-13 2003-04-10 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Novel selenocystein-containing proteins
JP2010509222A (ja) 2006-11-02 2010-03-25 ダニエル・ジェイ・カポン 可動部を備えたハイブリッド免疫グロブリン

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Fleckenstein, J. M. et al.，Database GenBank [online], Accession No. AQAP01000376 REGION: 44203..45174，2013年04月08日，https://www.ncbi.nlm.nih.gov/nuccore/AQAP01000376.1?from=44203&to=45174，Escherichia coli P0304777.5 gecP03047775.contig.386, whole genome shotgun sequence, product: cysteine synthase A
Mueller S. et al.，The formation of diselenide bridges in proteins by incorporation of selenocysteine residues: biosynthesis and characterization of (Se)2-thioredoxin，Biochemistry，Vol.33，1994年，p.3404-3412

Also Published As

Publication number Publication date
EP3481945A1 (en) 2019-05-15
EP3481945A4 (en) 2020-07-29
JP2023060001A (ja) 2023-04-27
JP2019520083A (ja) 2019-07-18
CA3030163A1 (en) 2018-01-18
WO2018013481A1 (en) 2018-01-18
CN109844105A (zh) 2019-06-04
US11155804B2 (en) 2021-10-26
US20190316110A1 (en) 2019-10-17

Similar Documents

Publication Publication Date Title
JP2023060001A (ja) セレノシステインを含む組換えポリペプチド及びその製造方法
TWI732176B (zh) 全人源的抗ｂ細胞成熟抗原（ｂｃｍａ）單鏈抗體及其應用
CN103429737B (zh) 细胞毒诱导治疗剂
JP6932693B2 (ja) カッパ及びラムダ軽鎖を含む抗原結合ポリペプチド構築物及びその使用
KR102168733B1 (ko) 중쇄와 경쇄의 회합이 제어된 항원 결합 분자
EP2655624B1 (en) Linker peptides and polypeptides comprising same
CN101687926A (zh) 中和抗体
WO2020135426A1 (zh) 全人源的抗cd30单链抗体及其应用
CN115667300A (zh) 猫抗体变异体
JP2020099341A (ja) ヒト抗体を産生する細胞
JP2017512772A (ja) Ｌｇ１〜３に特異的な抗ラミニン４抗体
CN116802205A (zh) 犬抗体恒定区中的突变
US12428496B2 (en) Antibodies, compositions for use in detecting or capturing a polypeptide in a sample, and methods for detecting or capturing a polypeptide in a sample
HK40008291A (en) Recombinant polypeptides comprising selenocysteine and method for producing the same
CN115956124A (zh) 改善蛋白质表达的方法
CN110475789A (zh) 抗平足蛋白抗体
KR20230030577A (ko) Fc 감마 RIIb에 대해 선택적인 이종이량체 Fc 변이체
CN120424216A (zh) 一种抗cd123抗体及其用途
JP2022060048A (ja) 抗体及びその使用
TW202535947A (zh) ＣＤ３３抗體、ＣＤ３３／Ｖδ２多特異性抗體及其用途
CN121057745A (zh) 具有修饰的fc区的双特异性抗假单胞菌抗体及其使用方法
CN119365494A (zh) 犬抗体突变体
WO2024229245A2 (en) High throughput fab antibody fragment generation and ranking
KR20240007767A (ko) CLDN18.2에 대한 단클론 항체 및 이의 Fc 조작된 형태
HK1259059B (en) Antigen-binding molecule having regulated conjugation between heavy-chain and light-chain

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200709

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20200709

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20210408

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210512

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210812

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220202

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220428

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220704

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20221031

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230228

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20230228

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20230307

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20230308

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230331

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230417

R150 Certificate of patent or registration of utility model

Ref document number: 7269648

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150