JP7222117B2 - Tuba1cタンパク質を過発現するエクソソーム基盤の癌の診断又は予後予測用マーカー組成物 - Google Patents
Tuba1cタンパク質を過発現するエクソソーム基盤の癌の診断又は予後予測用マーカー組成物 Download PDFInfo
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Description
5種の肺癌細胞株(H522、A549、H1650、PC9、H1299)、胸腺癌細胞株、及び食道癌細胞株をそれぞれ直径150mmのディッシュで培養した。ここで、超高速遠心分離機を用いて120、000gで4時間の間に遠心分離してエクソソームを除去したFBS(Fetal Bovine Serum)の上層液を培養液として使用した。前記培養液を用いて細胞が70~80%confluency状態になるよう2~3日間連続培養した。
前記実施形態1で選別したGCC2及びTUBA1Cタンパク質を含むエクソソームが肺癌の診断又は予後予測用マーカーとして利用可能性があるか否かを確認するために、正常群(n=3)と肺癌患者群(n=5)の血液で抽出したエクソソームからGCC2及びTUBA1Cの発現レベルをELISA(Enzyme linked immunoassay)分析を通じて確認した。
まず、正常被験者5人、食道癌患者5人の血漿(plasma)試料からエクソソームを抽出した後、TUBA1C ELISA KIT(Mybiosource社のTUBA1C ELISA KIT(Cat No.MBS9336377))を用いて試料内のTUBA1Cタンパク質濃度を確認した。
癌の診断のために、TUBA1C及びGCC2をニ重バイオマーカーとして用いる場合の有用性を評価するために、TUBA1C及びGCC2を単独に使用する場合と、2つのマーカーを併用する場合の診断敏感度変化をROCカーブで確認した。具体的に、正常被験者7名、肺癌患者21人の血漿からエクソソームを抽出した後、GCC2ELISA KIT、TUBA1C ELISA KITを用いてエクソソーム内のGCC2、TUBA1Cタンパク質の濃度を求めた後、統計的にROCカーブを用いてAUC値を確認し、これについて図5に示した。
Claims (8)
- TUBA1C(Tubulin alpha-1C chain)タンパク質の過発現エクソソームを含み、
前記エクソソームは、GCC2(GRIP and coiled-coil domain-containing protein)タンパク質をさらに含む、癌の診断又は予後予測用マーカー組成物。 - 前記癌は、肺癌、胸腺癌又は食道癌である、請求項1に記載の癌の診断又は予後予測用マーカー組成物。
- エクソソーム内のTUBA1C遺伝子に特異的に結合するプライマー又はプローブ、及びエクソソーム内のTUBA1Cタンパク質に特異的に結合する抗体のいずれか1つ以上、及び
エクソソーム内のGCC2遺伝子に特異的に結合するプライマー又はプローブ、及びエクソソーム内のGCC2タンパク質に特異的に結合する抗体のいずれか1つ以上を含む、癌の診断又は予後予測用組成物。 - 前記癌は、肺癌、胸腺癌又は食道癌である、請求項3に記載の癌の診断又は予後予測用組成物。
- 請求項3に記載の組成物を含む、癌の診断又は予後予測用キット。
- 前記キットは、RT-PCRキット、マイクロアレイチップキット、DNAキット、及びタンパク質チップキットからなる群より選択される1つ以上である、請求項5に記載の癌の診断又は予後予測用キット。
- 生物学的試料から分離したエクソソーム内のTUBA1C遺伝子又はタンパク質の発現レベルを測定するステップ、及び
前記エクソソーム内のGCC2遺伝子又はタンパク質の発現レベルを測定するステップを含む、癌の診断又は予後予測に必要な情報を提供する方法。 - 前記生物学的試料は、全血、血清、血漿、唾液、尿、喀痰、リンパ液、及び細胞からなる群より選択される1つ以上である、請求項7に記載の癌の診断又は予後予測に必要な情報を提供する方法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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KR10-2019-0039052 | 2019-04-03 | ||
KR20190039052 | 2019-04-03 | ||
KR10-2020-0040887 | 2020-04-03 | ||
PCT/KR2020/004589 WO2020204665A1 (ko) | 2019-04-03 | 2020-04-03 | Tuba1c 단백질을 과발현하는 엑소좀 기반 암 진단 또는 예후 예측용 마커 조성물 |
KR1020200040887A KR102350603B1 (ko) | 2019-04-03 | 2020-04-03 | Tuba1c 단백질을 과발현하는 엑소좀 기반 암 진단 또는 예후 예측용 마커 조성물 |
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JP2022527983A JP2022527983A (ja) | 2022-06-07 |
JP7222117B2 true JP7222117B2 (ja) | 2023-02-14 |
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US20190093172A1 (en) | 2016-04-15 | 2019-03-28 | Exosome Diagnostics, Inc. | Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer |
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JP2013526852A (ja) * | 2010-04-06 | 2013-06-27 | カリス ライフ サイエンシズ ルクセンブルク ホールディングス | 疾患に対する循環バイオマーカー |
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KR20160133740A (ko) * | 2015-05-13 | 2016-11-23 | 경북대학교 산학협력단 | 파이브로넥틴 단백질 양성 엑소좀을 포함하는 암 진단 또는 예후 예측용 조성물 |
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US20190093172A1 (en) | 2016-04-15 | 2019-03-28 | Exosome Diagnostics, Inc. | Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer |
Non-Patent Citations (1)
Title |
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Stephanie N Hurwitz et al,Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers,Oncotarget,2016年11月24日,vol.7, No.52,86999-87015 |
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US20220154283A1 (en) | 2022-05-19 |
JP2022527983A (ja) | 2022-06-07 |
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