JP7215808B2 - 操作された免疫調節エレメントおよび変更された免疫 - Google Patents

操作された免疫調節エレメントおよび変更された免疫 Download PDF

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JP7215808B2
JP7215808B2 JP2019507817A JP2019507817A JP7215808B2 JP 7215808 B2 JP7215808 B2 JP 7215808B2 JP 2019507817 A JP2019507817 A JP 2019507817A JP 2019507817 A JP2019507817 A JP 2019507817A JP 7215808 B2 JP7215808 B2 JP 7215808B2
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nucleic acid
gene
cells
sequence
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JP2019524140A5 (enExample
JP2019524140A (ja
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ジュン キム、セオク
キム、ヨーン-ヨン
ユ、ホ-スン
ジュン、イン-ヨン
ミン リー、ジュン
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Toolgen Inc
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Toolgen Inc
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Priority claimed from PCT/KR2017/008835 external-priority patent/WO2018030874A1/ko
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Priority to JP2023004738A priority Critical patent/JP2023052426A/ja
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024113020A (ja) * 2016-08-12 2024-08-21 ツールゲン インコーポレイテッド 操作された免疫調節エレメントおよび変更された免疫

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
CN105939767B (zh) 2014-01-08 2018-04-06 弗洛设计声能学公司 具有双声电泳腔的声电泳装置
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US12275963B2 (en) 2017-05-08 2025-04-15 Toolgen Incorporated Artificially manipulated immune cell
CN107164377A (zh) * 2017-06-12 2017-09-15 王小平 基于碱基编辑的基因敲除方法及其应用
US11441135B2 (en) 2017-07-07 2022-09-13 Toolgen Incorporated Target-specific CRISPR mutant
CN112040986A (zh) * 2018-03-15 2020-12-04 Ksq治疗公司 用于改进的免疫疗法的基因调控组合物和方法
CN110904045A (zh) * 2018-09-17 2020-03-24 中国科学院动物研究所 经修饰的t细胞、其制备方法及用途
CN111321171A (zh) * 2018-12-14 2020-06-23 江苏集萃药康生物科技有限公司 一种应用CRISPR/Cas9介导ES打靶技术制备基因打靶动物模型的方法
CN110373385A (zh) * 2019-07-12 2019-10-25 赛德特生物科技开发有限公司 改善神经细胞功能的免疫细胞培养基及其制备方法与应用
PH12022550548A1 (en) * 2019-09-06 2023-11-20 Crispr Therapeutics Ag Genetically engineered t cells having improved persistence in culture
EP4501951A3 (en) * 2020-08-25 2025-04-30 Kite Pharma, Inc. T cells with improved functionality
KR20240004245A (ko) * 2021-02-10 2024-01-11 카테릭스 피티와이. 리미티드 기능이 향상된 줄기세포 유래 면역세포를 생성하는 방법 및 조성물
CN114807044B (zh) * 2021-04-30 2023-09-08 四川大学华西医院 一种具有高nkt细胞比例的car-cik细胞制备方法及应用
CN115947865A (zh) * 2021-09-18 2023-04-11 上海美丽人生医疗科技有限公司 一种嵌合抗原受体、表达构建物、car-t细胞及其应用
CN115894706A (zh) * 2021-09-30 2023-04-04 北京康辰药业股份有限公司 靶向PD-L1和FasL的融合蛋白、核苷酸序列及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014039513A2 (en) 2012-09-04 2014-03-13 The Trustees Of The University Of Pennsylvania Inhibition of diacylglycerol kinase to augment adoptive t cell transfer
WO2015090230A1 (en) 2013-12-19 2015-06-25 Novartis Ag Human mesothelin chimeric antigen receptors and uses thereof

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050244851A1 (en) * 2004-01-13 2005-11-03 Affymetrix, Inc. Methods of analysis of alternative splicing in human
US20050221354A1 (en) * 2004-02-18 2005-10-06 Wyeth Nucleic acid arrays for monitoring expression profiles of drug target genes
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
CN102333277B (zh) 2011-07-29 2014-08-06 上海合合信息科技发展有限公司 结合地理位置信息和蓝牙技术的电子名片传递方法及系统
US20130129668A1 (en) * 2011-09-01 2013-05-23 The Regents Of The University Of California Diagnosis and treatment of arthritis using epigenetics
ES2728782T3 (es) 2012-05-25 2019-10-28 Univ California Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN
WO2014059173A2 (en) 2012-10-10 2014-04-17 Sangamo Biosciences, Inc. T cell modifying compounds and uses thereof
KR102955556B1 (ko) * 2012-12-12 2026-04-22 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물
CN105209621B (zh) * 2012-12-12 2021-05-25 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
SG10201707569YA (en) * 2012-12-12 2017-10-30 Broad Inst Inc Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications
MX380562B (es) 2012-12-12 2025-03-12 Broad Inst Inc Modificaciones de sistemas, métodos y composiciones guía optimizadas para la manipulación de secuencias.
US11311575B2 (en) * 2013-05-13 2022-04-26 Cellectis Methods for engineering highly active T cell for immunotherapy
EP2997146A4 (en) 2013-05-15 2017-04-26 Sangamo BioSciences, Inc. Methods and compositions for treatment of a genetic condition
JP6608807B2 (ja) 2013-05-29 2019-11-20 セレクティス Rnaガイドcasヌクレアーゼ系を用いることによって免疫療法のためにt細胞を操作するための方法
CA2912389C (en) * 2013-06-10 2020-01-28 The General Hospital Corporation D/B/A Massachusetts General Hospital Methods and compositions for reducing immunosupression by tumor cells
DK3105317T3 (en) 2014-02-14 2019-01-14 Cellectis Immunotherapy cells that are genetically engineered to target antigen on both immune cells and pathological cells
CN122399016A (zh) * 2014-03-15 2026-07-17 诺华股份有限公司 使用嵌合抗原受体治疗癌症
US10738278B2 (en) 2014-07-15 2020-08-11 Juno Therapeutics, Inc. Engineered cells for adoptive cell therapy
CN106536721B (zh) * 2014-08-06 2020-12-04 车医科学大学校产学协力团 核酸酶介导的编辑编码hla的基因所产生的免疫相容性细胞
KR102546296B1 (ko) 2014-10-31 2023-06-21 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 변형된 t 세포에서 유전자 발현의 변경 및 그의 용도
JP6190995B2 (ja) 2014-11-17 2017-09-06 国立大学法人 東京医科歯科大学 簡便で高効率の遺伝子改変非ヒト哺乳動物の作製方法
US20190388469A1 (en) 2015-01-30 2019-12-26 The Regents Of The University Of California Protein delivery in primary hematopoietic cells
CN107787367B (zh) * 2015-04-06 2021-10-26 里兰斯坦福初级大学理事会 用于crispr/cas介导的基因调控的化学修饰的引导rna
WO2018030874A1 (ko) 2016-08-12 2018-02-15 주식회사 툴젠 조작된 면역조절요소 및 이에 의해 변형된 면역 활성
US12012598B2 (en) 2016-08-12 2024-06-18 Toolgen Incorporated Manipulated immunoregulatory element and immunity altered thereby
WO2018068053A2 (en) 2016-10-07 2018-04-12 Integrated Dna Technologies, Inc. S. pyogenes cas9 mutant genes and polypeptides encoded by same
EP3336107A1 (en) 2016-12-15 2018-06-20 Miltenyi Biotec GmbH Immune cells expressing an antigen binding receptor and a chimeric costimulatory receptor
US12275963B2 (en) 2017-05-08 2025-04-15 Toolgen Incorporated Artificially manipulated immune cell
US12467050B2 (en) 2017-10-02 2025-11-11 Georgia Tech Research Corporation Methods and compositions for engineering synthetic bioswitches for remote control of biological activity
BR112020011223A2 (pt) 2017-12-08 2020-11-17 Juno Therapeutics Inc marcadores fenotípicos para terapia celular e mé-todos relacionados
US12473533B2 (en) 2018-07-09 2025-11-18 The Regents Of The University Of California Gene targets for T-cell-based immunotherapy

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014039513A2 (en) 2012-09-04 2014-03-13 The Trustees Of The University Of Pennsylvania Inhibition of diacylglycerol kinase to augment adoptive t cell transfer
WO2015090230A1 (en) 2013-12-19 2015-06-25 Novartis Ag Human mesothelin chimeric antigen receptors and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Cancer Res,73(12),2013年,p3566-3577

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024113020A (ja) * 2016-08-12 2024-08-21 ツールゲン インコーポレイテッド 操作された免疫調節エレメントおよび変更された免疫

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