JP7195655B2 - Effervescent composition with antiviral activity - Google Patents

Description

TECHNICAL FIELD The present invention relates to a foaming composition that has an antiviral effect and has the property of foaming when mixed with air. More specifically, the present invention is an acidic antiviral composition with a high alcohol content, and suppresses the decrease (disappearance) of foamability (foamability) during storage, thereby stabilizing foaming. It relates to a foamable composition that can be developed (foamed) by According to the foaming composition, alcoholic foam can be obtained stably and can be used for cleaning or disinfection.

Hand hygiene using alcohol-based hand sanitizers is an important means of preventing healthcare-associated infections through the hands, but the effect against non-enveloped viruses such as norovirus is not necessarily sufficient. (Non-Patent Documents 1-3).

In recent years, it has been reported that adjusting the pH of the alcohol solution to an acidic or alkaline range increases the inactivation effect against non-enveloped viruses (Non-Patent Documents 4-6). Alcohol-based hand sanitizers that are also effective have been put into practical use in Japan.

Such alcohol-based hand sanitizers are usually in the form of aqueous alcohol solutions (including spray liquids), and are used by dropping or spraying them on the palms and backs of the hands and rubbing the hands together to evaporate and dry the alcohol component. . However, in the case of an alcohol aqueous solution, there is a problem that the necessary amount of disinfectant cannot be taken on the skin or palm, and even if it is taken, it easily overflows from the hand. For this reason, a method of adding a thickening agent to increase the viscosity (thickening or gelling) or imparting foaming properties to form a foam has been proposed.

However, when preparing the latter foaming alcohol-based disinfectant, if a relatively high concentration of alcohol is blended in order to exert a disinfecting effect, the foaming property will be reduced or impaired, and it will not foam, or even if it foams. There is a problem that the durability is low and the bubbles disappear quickly (see Patent Documents 1 and 2, etc.). Moreover, if the pH of the alcohol-based disinfectant is low, its foamability may be impaired during storage.

Kenji Yamazaki. Virus inactivation measures using alcohol-based disinfectants. Inspection and technology, 31 (5), 430-431. (2003) Doultree JC, Druce JD, Birch CJ, Bowden DS, Marshall JA. Inactivation of feline calicivirus, a Norwalk virus surrogate. J Hosp Infect, 41(1):51-57. (1999) Lages, S. L. S., Ramakrishnan, M. A., and Goyal, S. M. In-vivo efficacy of hand sanitizers against feline calicivirus: a surrogate for norovirus. J. Hosp. Infect., 68, 159-163. (2008) Kramer, A.S. et al. Virucidal activity of a new hand disinfectant with reduced ethanol content: comparison with other alcohol-based formulation. J. Hosp. Infect., 62, 98-106. (2006) Macinga D. R., Sattar S. A., Jaykus L. A., Arbogast J. W. Improved inactivation of nonenveloped enteric viruses and their surrogates by a novel alcohol-based hand sanitizer. Appl. Environ. Microbiol., 74, 5047-5052. (2008) Kumashita, Y., Harada, Y., Takamoto, K., Murata, Y., Furuta, T., Nishio, O. Inactivation effect of feline calicivirus using viral infection titer and real-time PCR gene quantification. 871-877 (2009) Tree JA, Adams MR, Lees DN. Disinfection of feline calicivirus (a surrogate for Norovirus) in wastewaters. J Appl Microbiol, 98(1), 155-162. (2005) Davies JG, Babb JR, Bradley CR, Ayliffe GAJ. Preliminary study of test methods to assess the virucidal activity of skin disinfectants using poliovirus and bacteriophages. J Hosp Infect, 25, 125-131. (1993) Wobus CE, Thackray LB, Virgin IV HW. Murine Norovirus: a Model System To Study Norovirus Biology and Pathogenesis. J Virol, 80(11), 5104-5112. (2006) Matsumura, R., Nakamura, E., Noguchi, Y., Kumamoto, Y., Yamamoto, M., Furuta, T. Efficacy evaluation of viricidal alcohol-based hand sanitizers.

JP 2008-524295 A JP 2013-253091 A

The present invention has been developed to solve the above-described conventional problems. Specifically, the present invention is an alcohol-containing foaming composition used for cleaning or disinfecting that stably produces (foams) an alcoholic foam even when stored for a relatively long period of time. An object of the present invention is to provide a foamable composition having excellent storage stability, characterized by being able to That is, an object of the present invention is to provide an effervescent composition having an antiviral effect and stable foamability (foamability), particularly an effervescent composition containing alcohol at a high concentration.

The inventors of the present invention have made extensive studies to solve the above problems, and found that the alcohol concentration is 50% by volume, preferably 60% by volume or more, and the pH of the final composition is 5 or less. By using a silicone-based surfactant containing bis-PEG/PPG-[14-20]/[5-20]-dimethicone as a surfactant, a relatively It was found that alcoholic foam can be stably generated even after long-term storage, that is, a foaming composition having good antiviral action and foaming property (foaming property) can be obtained. It was completed.

The present invention has been completed based on such knowledge, and has the following embodiments.

(I) an antiviral effervescent composition (I-1) (A) an alcohol present in an amount of 50-90% by volume of the total composition;
(B) a silicone-based surfactant comprising bis-PEG/PPG-[14-20]/[5-20]-dimethicone in an amount of 0.1-10% by weight of the total composition; and (C) A foamable composition comprising water present in an amount to make up 100% by weight of the total composition and having a pH of 5 or less, preferably in the range of pH 2-5.
(I-2) The above component (A) is a lower alcohol having 1 to 4 carbon atoms, preferably at least one lower alcohol selected from the group consisting of ethanol, 1-propanol, and 2-propanol (I -1) The foamable composition described in 1).
(I-3) The component (A) is present in an amount of 60 to 90% by volume, preferably 60 to 85% by volume, more preferably 60 to 80% by volume of the total composition (I-1) or ( The foamable composition described in I-2).
(I-4) Bis-PEG/PPG-[14-20]/[5-20]-dimethicone contained in component (B) above is bis-PEG/PPG-20/20-dimethicone, bis-PEG/ At least one selected from the group consisting of PPG-14/14-dimethicone, bis-PEG/PPG-15/5-dimethicone, and bis-PEG/PPG-18/6-dimethicone (I-1) to The foamable composition according to any one of (I-3).
(I-5) Bis-PEG/PPG-[14-20 in an amount of 0.1 to 5% by weight, preferably 0.1 to 2% by weight, more preferably 0.5 to 2% by weight of the total composition. ]/[5-20]-dimethicone, the foaming composition according to any one of (I-1) to (I-4).
(I-6) The foamable composition according to any one of (I-1) to (I-5), wherein the component (B) further contains bis-PEG-[8-20]-dimethicone.
(I-7) The effervescent composition according to any one of (I-1) to (I-6), further comprising at least one selected from the group consisting of acids and salts thereof for adjusting pH. thing.
(I-8) the acid is hydrochloric acid, sulfuric acid, nitric acid, acetic acid, propionic acid, sulfamic acid, formic acid, gluconic acid, ascorbic acid, ferulic acid, succinic acid, phytic acid, lactic acid, citric acid, malic acid, phosphoric acid, The effervescent composition according to (I-7), which is at least one selected from the group consisting of maleic acid, malonic acid, caprylic acid, adipic acid, and fumaric acid.
(I-9) The foamable composition of any of (I-1) through (I-8), further comprising a foam stabilizer present in an amount of about 0.01 to 12% by weight or less.
(I-10) The foam stabilizer is at least one selected from the group consisting of glycol ether, glycerin, butylene glycol, behentrimonium chloride, hydrophobized hydroxypropylmethylcellulose, hypromellose, cetrimonium chloride, ethylcellulose and methylcellulose. The foamable composition according to any one of (I-1) to (I-9).
(I-11) Allantoin, diisobutyl adipate, glycerin, propylene glycol, 1,3-butylene glycol, myristic acid, as moisturizing agents and/or emollients, further present in a total amount of about 0.05 to 5% by mass or less. selected from the group consisting of isopropyl, lanolin, vinyl alcohol, polyvinylpyrrolidone, glyceryl oleate and sorbitol, cocoglucoside, cetyl alcohol, stearyl alcohol, lauryl alcohol, myristyl alcohol, palmityl alcohol, cetyl alcohol, amino acids and amino acid derivatives The foamable composition according to any one of (I-1) to (I-10), comprising at least one.
(I-12) the composition is foamy when mixed with air, and a mixture of the composition and air forms foam when the composition is mixed with air; (I-1) The foamable composition according to any one of (I-11).
(I-13) The effervescent composition according to any one of (I-1) to (I-12), which has an antiviral effect.
(I-14) The virus is MS2 phage, feline calicivirus, murine norovirus (norovirus substitute), poliovirus, adenovirus, SV40, influenza virus, herpes virus, bovine viral diarrhea virus (hepatitis C virus substitute) and these The effervescent composition according to any one of (I-1) to (I-13), which is at least one selected from the group consisting of viruses replaced by viruses of (I-1) to (I-13).
(I-15) The effervescent composition according to any one of (I-1) to (I-14), which is an effervescent alcohol disinfectant composition.
(I-16) The foaming composition according to any one of (I-1) to (I-15), which is a composition for skin disinfection.
(I-17) The effervescent composition according to any one of (I-1) to (I-15), which is an environmental disinfection composition.
(I-18) The foamable composition according to any one of (I-1) to (I-17) contained in a discharge container having a foam dispensing device,
wherein the dispensing device comprises a foaming member configured to mix air with the foaming composition to form a foam as the foaming composition is dispensed from the dispensing vessel. The above foamable composition.
(I-19) The foamable composition according to (I-18), wherein the discharge container is a gas type discharge container or a non-gas type discharge container.

(II) Method for producing a foaming composition in a discharge container (II-1) The foaming composition of any one of (I-1) to (I-17) is placed in a discharge container having a foam dispensing device. A method for producing a foamable composition in a discharge container, comprising:
wherein the dispensing device comprises a foaming member configured to mix air with the foaming composition to form a foam as the foaming composition is dispensed from the dispensing vessel. The above manufacturing method.
(II-2) The manufacturing method according to (II-1), wherein the discharge container is a gas type discharge container or a non-gas type discharge container.

(III) Use of the effervescent composition having antiviral action (III-1) Use of the effervescent composition according to any one of (I-1) to (I-18) as a disinfectant.
(III-2) Use of the effervescent composition according to any one of (I-1) to (I-18) for disinfecting skin.
(III-3) Use of the effervescent composition according to any one of (I-1) to (I-18) for disinfecting an environment.

(IV) Method for imparting foamability maintenance function to foamable composition (IV-1) Bis-PEG/PPG-[14-20]/[5-20]-dimethicone to 0.1 to 10% by mass Furthermore, by blending with a composition of pH 2 to 5 containing alcohol and water to prepare an alcohol-containing foaming composition of 50 to 90% by volume, the foaming composition maintains foamability. how to give.
(IV-2) The method according to (IV-1), further comprising bis-PEG-[8-20]dimethicone.
(IV-3) The pH of the composition of pH 2 to 5 is adjusted with a pH adjuster selected from the group consisting of acids and salts thereof, (IV-1) or (IV-2 ).
(IV-4) The method according to any one of (IV-1) to (IV-3), wherein the alcohol-containing effervescent composition is a disinfecting composition having an antiviral effect.
(IV-5) The alcohol-containing foaming composition is prepared in a discharge container having a foam dispensing device,
wherein the dispensing device comprises a foaming member configured to mix air with the foaming composition to form a foam as the foaming composition is dispensed from the dispensing vessel. The method according to any one of (IV-1) to (IV-4).

According to the present invention, not only a pressurized distribution system (including a pressurized gas discharge container such as an aerosol container) that has an antiviral effect and uses a gas such as a propellant, but also a distribution system that does not use a gas (non-gas) Non-gassing delivery containers such as aerosol containers) can be used to provide high alcohol content foamable compositions that can be dispensed as a foam. In particular, the foamable compositions of the present invention are capable of producing stable foams even when dispensed from non-gas type discharge containers, and do not necessarily require the use of gas type discharge containers with propellants, although they may be used. It can be foamed in the same way.

The foamable composition of the present invention maintains its foamability (foamability) even when stored for a relatively long period of time, can be stably foamed by mixing with air, and has antibacterial properties. As an alcohol foam, it can be used for cleaning or disinfecting (including antibacterial) purposes.

(I) Effervescent Composition Having Antiviral Action The effervescent composition of the present invention is characterized by containing the following (A) to (C) and having a pH in the range of 5 or less:
(A) an alcohol present in an amount of 50-90% by volume of the total composition;
(B) a silicone-based surfactant comprising bis-PEG/PPG-[14-20]/[5-20]-dimethicone in an amount of 0.1-10% by weight of the total composition; and (C) Water present in an amount to bring the total composition to 100% by weight.

Each of these components will be described below.

(A) Alcohol The alcohol used in the present invention is a lower hydrocarbon chain alcohol. Specifically, lower alcohols having 1 to 6 carbon atoms, preferably 1 to 4 carbon atoms, such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, isobutyl alcohol, and tert-butyl alcohol. . Preferred alcohols are ethanol, 1-propanol and 2-propanol, more preferably ethanol. These alcohols may be used singly or in any combination of two or more.

In the foamable composition (100% by volume) of the invention, the alcohol can be present in the range of 50-90% by volume. From the viewpoint of antiviral properties, the lower limit is preferably 50% by volume or more, more preferably 60% by volume or more. Although the upper limit is not particularly limited to 90% by volume, it is preferably 85% by volume, more preferably 80% by volume. Specific ranges include, for example, 50 to 90% by volume, 60 to 90% by volume, 60 to 85% by volume, and 60 to 80% by volume.

(B) Silicone-based surfactant In the present invention, the term “silicone-based surfactant” means that the lipophilic chain has a silicone chain —(R ₂ Si—O) _n — (wherein R is a monovalent organic group is a surfactant or a mixture of such surfactants, and the composition containing it has foaming properties due to the surfactant.

Bis-PEG/PPG-[14-20]/[5-20]-dimethicone is a surfactant containing a silicone chain in the lipophilic chain.

Bis-PEG/PPG-[14-20]/[5-20]-dimethicone has 14 to 20 polyethylene oxide repeating units corresponding to [PEG] on both sides of the silicone main chain, and It is a silicone surfactant having 5 to 20 polypropylene oxide repeating units corresponding to [PPG].

The number of repetitions of [PEG] is not limited as long as it is in the range of 14-20 as described above, but is preferably 16-20, more preferably 18-20, and particularly preferably 20.

The number of repetitions of [PPG] is not limited as long as it is in the range of 5 to 20 as described above, but is preferably 10 to 20, more preferably 14 to 20, and particularly preferably 20.

As bis-PEG/PPG-[14-20]/[5-20]-dimethicone, preferably bis-PEG/PPG-14/14-dimethicone, bis-PEG/PPG-15/5-dimethicone, bis-PEG /PPG-18/6-dimethicone and bis-PEG/PPG-20/20-dimethicone can be mentioned. Bis-PEG/PPG-20/20-dimethicone is more preferred.

One of these bis-PEG/PPG-[14-20]/[5-20] dimethicone may be used alone, or any bis-PEG/PPG-[14-20]/[5- 20]-Dimethicone can also be used in combination of two or more.

In the foamable composition (100% by weight) of the present invention, the bis-PEG/PPG-[14-20]/[5-20]-dimethicone can be present in the range of 0.1-10% by weight. . From the viewpoint of foaming stability, the lower limit is preferably 0.5% by mass or more, more preferably 1% by mass or more. As described above, the upper limit of bis-PEG/PPG-[14-20]/[5-20]-dimethicone is not particularly limited to 10% by mass, but is preferably 5% by mass, more preferably 2% by mass. %. The blending amount of bis-PEG/PPG-[14-20]/[5-20]-dimethicone per 100% by mass of the foamable composition of the present invention is preferably the blending ratio of alcohol contained in the foamable composition. can be adjusted as appropriate. Specifically, when the blending ratio of alcohol in the foamable composition is 50 to 60% by volume in 100% by volume of the foamable composition, bis-PEG/PPG-[14-20]/[5-20] - Dimethicone can be blended in the range of 0.1 to 10% by weight, preferably in the range of 1 to 2% by weight, based on 100% by weight of the foamable composition. In addition, when the blending ratio of alcohol in the foamable composition is more than 60% by volume and 90% by volume or less in 100% by volume of the foamable composition, bis-PEG/PPG-[14-20]/[5-20 ]-Dimethicone can be blended in the range of 0.5 to 10% by mass, preferably in the range of 1 to 2% by mass, based on 100% by mass of the foamable composition.

In order to exhibit a particularly high antiviral effect, the alcohol content in the effervescent composition is preferably about 80% by volume (80 ± 5% by volume), and in this case, the antiviral effect is not impaired. The amount of bis-PEG/PPG-[14-20]/[5-20]-dimethicone for exhibiting foaming stability is 0.5 to 10% by mass in 100% by mass of the foaming composition. , preferably 1 to 2% by mass.

A silicone-based surfactant other than bis-PEG/PPG-[14-20]/[5-20]-dimethicone may be blended with the silicone-based surfactant to the extent that it does not interfere with the effects of the present invention. can.

Silicone-based surfactants that can be used in combination with bis-PEG/PPG-[14-20]/[5-20]-dimethicone in the present invention preferably include silicone polyethers. Such silicone polyethers include multipendant silicone surfactants and linear bifunctional silicone surfactants, preferably linear bifunctional silicone surfactants. A specific example of such a linear bifunctional silicone surfactant is bis-PEG-[8-20]dimethicone.

Bis-PEG-[8-20]dimethicone is a silicone surfactant having 8 to 20 repeating units of polyethylene oxide corresponding to [PEG] on both sides of the silicone backbone.

The number of repetitions of [PEG] is not limited as long as it is in the range of 8-20 as described above, but it is preferably in the range of 8-12, more preferably in the range of 10-12. Bis-PEG-[8-20] dimethicone, preferably bis-PEG-10 dimethicone, bis-PEG-12 dimethicone, bis-PEG-14 dimethicone, bis-PEG-17 dimethicone, bis-PEG-20 dimethicone, etc. of bis-PEG-[10-20]dimethicone. Bis-PEG-10 dimethicone, bis-PEG-12 dimethicone and bis-PEG-14-dimethicone are more preferred, and bis-PEG-12 dimethicone is particularly preferred.

One of these bis-PEG-[8-20] dimethicones may be used alone, or any two or more of bis-PEG-[8-20] dimethicones may be used in combination. can. Furthermore, in addition to bis-PEG-[8-20] dimethicone, other than bis-PEG/PPG-[14-20]/[5-20]-dimethicone, as long as it does not impair the effects of the present invention can also be used in combination with a silicone surfactant.

When bis-PEG/PPG-[14-20]/[5-20]dimethicone and bis-PEG-[8-20]dimethicone are used in combination, bis-PEG-[8-20]dimethicone is the foaming agent of the present invention. It can be present in the range of 0.01 to 10% by weight per 100% by weight of the composition. The lower limit thereof is preferably 0.5% by mass or more. Moreover, the upper limit thereof is preferably 5% by mass.

(C) Water The foaming composition of the present invention is an aqueous composition containing water as a solvent.

As mentioned above, the foamable compositions of the present invention are capable of producing foam without the use of pressurized containers or propellants, and have antiviral activity and are used for cleaning, disinfecting or antimicrobial treatments. . Therefore, the water used as the solvent is required not to impair these effects (foaming properties, antiviral properties).

Specifically, the water used in the present invention should have a hardness of less than 120 mg/L (soft water, moderately soft water) so as not to impair the foaming properties of the foaming composition of the present invention. desirable. Here, the hardness can be indicated by a value (mg/L) obtained by converting the concentration of calcium salts and magnesium salts (total hardness) contained in water into the concentration of calcium carbonate (American hardness). For example, the following formula can be used as a method for converting the amount of calcium salt (Ca=40) and magnesium salt (Mg=24.3) contained in water to the amount of calcium carbonate (CaCO ₃ =100).

[Number 1]
Hardness (mg/L) ≒ calcium concentration x (100/40) + magnesium concentration x (100/24.3)

Water hardness is preferably 100 mg/L or less.

Also, the water used in the present invention is purified water. Here, purified water refers to water that has been purified by adding the necessary water treatment operations to raw water (raw water and groundwater), preferably as tap water. Those satisfying the above conditions can be mentioned. Specifically, tap water, ion-exchanged water, purified water, alkaline ion water, hydrogen water, deep sea water, and the like can be mentioned. Purified water or ion-exchanged water is preferred.

Water can be blended in an amount that makes the total amount of the foamable composition of the present invention 100% by mass.

(D) pH of the effervescent composition
The foamable composition of the present invention is characterized by being adjusted to be acidic.

Specifically, the foamable composition of the present invention preferably has a pH of 5 or less, preferably 4 or less. Although the lower limit of pH is not limited, it is usually pH 1 or higher, preferably pH 2 or higher, and more preferably pH 3 or higher. A suitable pH range includes pH 1-5, more preferably pH 2-5, and more preferably pH 4-5.

As a method for adjusting the pH of the foamable composition of the present invention to the above pH, addition of a pH adjuster can be usually mentioned. Acids and/or salts thereof can be used as pH adjusters. Specifically, inorganic acids such as hydrochloric acid, sulfuric acid, or nitric acid; Organic acids such as acid, phosphoric acid, maleic acid, malonic acid, caprylic acid, adipic acid, or fumaric acid can be mentioned, and these may be used singly or in combination of two or more. They can also be used in combination. Preferred are lactic acid, gluconic acid, succinic acid, citric acid, malic acid and phosphoric acid, and more preferred are organic acids such as lactic acid, citric acid, malic acid and phosphoric acid.

The acid salt may be one that forms a salt with the above acid, and examples thereof include alkali metal salts such as sodium and potassium, and alkaline earth salts such as magnesium and calcium. Alkali metals such as sodium are preferred.

(E) Other Components The foamable composition of the present invention may contain other components as long as they do not impair the effects of the present invention. Such other ingredients include third surfactants other than the surfactants described above, foam stabilizers, antibacterial agents, thickeners, cosmetic ingredients (e.g., moisturizers, emollients, etc.), preservatives, perfumes, pigments. (colorant), dissolution aid, complex-forming agent, sequestering agent and the like can be exemplified without limitation.

Third surfactants include fluorinated surfactants, alkyl glucosides, poly(ethoxylated and/or propoxylated) alcohols, poly(ethoxylated and/or propoxylated) esters, poly(ethoxylated and/or or propoxylated) alcohol derivatives, poly(ethoxylated and/or propoxylated) ester derivatives, alkyl alcohols, alkenyl alcohols, esters of polyhydric alcohols, ethers of polyhydric alcohols, polyalkoxylated derivatives of polyhydric alcohols esters of polyhydric alcohols, ethers of polyalkoxylated derivatives of polyhydric alcohols, sorbitan fatty acid esters, polyalkoxylated derivatives of sorbitan fatty acid esters, betaines, sulfobetaines, imidazoline derivatives, amino acid derivatives, lecithins, phosphatides, some amine oxides and sulfoxides, and mixtures thereof.

A preferred fluorinated surfactant is DEA C[8-18]perfluoroalkylethyl phosphate. Another preferred fluorinated surfactant is ammonium C[6-16] perfluoroalkylethyl phosphate. Preferred polyethoxylated fatty alcohols are polyethoxylated stearyl alcohol (21 moles of ethylene oxide) and polyethoxylated stearyl alcohol (2 moles of ethylene oxide), and mixtures of the two.

A preferred betaine is cocamidopropyl betaine.

A preferred alkylglucoside is cocoglucoside.

Fluorosurfactants that may be included include (a)-(c) or mixtures thereof:
(a) Amphoteric polytetrafluoroethyleneacetoxypropyl betaine represented by the following formula ^: _CF3CF2 ( _CF2CF2 ) _{nCH2CH2 (} OAc) _{CH2N + ( CH3} ) _2CH2COO ^- _{( formula} medium, n=2~4);
(b) an ethoxylated nonionic fluorosurfactant of the formula:
_{RfCH2CH2O ( CH2CH2O ) xH}
(wherein Rf=F( _CF2CF2 ) _y , x= ₀ to about 15, y=1 to about 7);
(c) an anionic phosphate fluorosurfactant of the formula:
( _RfCH2CH2O )xP ₍ O)( _{ONH4 ) y}
(wherein Rf=F( _CF2CF2 ) _z , x=1 or ₂ , y=2 or 1, x+y=3, z=1 to about 7).

A foam stabilizer in the context of the present invention increases the amount of foam generated by the foamable composition of the present invention and/or increases the persistence of the foam generated by the foamable composition of the present invention. Or, it refers to an additive that has the effect of suppressing the decrease [disappearance] over time. Examples of foam stabilizers include, but are not limited to, xanthan gum, guar gum, agar, thickening polysaccharides such as alginic acid or salts thereof; carboxymethylcellulose, hydroxypropylcellulose, hypromellose, hydrophobized hydroxypropylmethylcellulose, alkylcellulose. (e.g. methylcellulose, ethylcellulose), cellulose mixed ethers, carboxymethylhydroxyethylcellulose, ethylhydroxyethylcellulose, methoxyhydroxyalkylcellulose, and methylhydroxyalkylcellulose (e.g. methylhydroxyethylcellulose, methylhydroxypropylcellulose, methylhydroxybutylcellulose). Cellulose derivatives such as; Stearate, sodium stearoyl lactylate and the like can be exemplified. Glycol ether, glycerin, butylene glycol, behentrimonium chloride, cetrimonium chloride, hypromellose, hydrophobized hydroxypropylmethylcellulose, and alkylcellulose (eg, methylcellulose, ethylcellulose) are preferred. These may be used individually by 1 type, and can also be used in arbitrary combinations of 2 or more types.

These foam stabilizers are optional ingredients. When blending such a foam stabilizer, its content is 0.01 to 20% by mass, preferably 0.01 to 10% by mass, more preferably 0.05 to 3%, per 100% by mass of the foamable composition of the present invention. Mass % ranges can be mentioned.

As described above, the effervescent composition of the present invention does not require an antibacterial agent because the high-concentration alcohol component contained therein acts as a disinfecting substance (antiviral substance, antibacterial substance). However, as long as the effects of the present invention are not impaired, the addition of the antibacterial agent is not restricted. Specific examples of antibacterial agents include chlorhexidine salts, iodine, complex forms of iodine, parachlorometaxylenol, triclosan, hexachlorophene, phenol, behenyl alcohol, surfactants having long-chain hydrophobic groups and quaternary groups, Mention may be made of hydrogen oxide, silver, silver salts, silver oxides, other quaternary ammonium salts and mixtures thereof. Preferred are chlorhexidine gluconate, didecyldimethyldiammonium chloride, benzalkonium chloride and behenyl alcohol. These may be used individually by 1 type, and can also be used in arbitrary combinations of 2 or more types. As noted above, these antimicrobial agents are optional. When such an antibacterial agent is blended, the content thereof is in the range of 0.01 to 5% by mass, preferably 0.05 to 3% by mass, per 100% by mass of the foamable composition of the present invention.

Humectants and/or emollients that can be used in the foaming composition of the present invention include allantoin, diisobutyl adipate, isopropyl myristate, lanolin, vinyl alcohol, polyvinylpyrrolidone, polyols (e.g. glycerin, propylene glycol, butylene glycol, glyceryl). oleate, or sorbitol), polyethylene glycol, cocoglucoside, fatty alcohols (cetyl alcohol, stearyl alcohol, lauryl alcohol, myristyl alcohol, alkoxylated cetyl alcohol, or palmityl alcohol), and ceteareth 20, despanthenol, sodium hyaluronate. , urea, chamomile extract and the like. These may be used individually by 1 type, and can also be used in arbitrary combinations of 2 or more types. In the present invention, the term "emollient agent" broadly refers to materials that can maintain or improve skin moisture level, compliance, or appearance when repeatedly applied to the skin of fingers and the like. In this sense, it also includes humectants. These ingredients are optional ingredients. When blending such moisturizing agents and/or emollients, the content should be in the range of 0.01 to 5% by mass, preferably 0.05 to 3% by mass, per 100% by mass of the foaming composition of the present invention. can be done.

(F) Foamable Composition Preferred embodiments of the foamable composition of the present invention include the following foamable compositions:
(a) (A) 50-90% by volume ethanol, (B) 0.1-10% by weight bis-PEG/PPG-[14-20]/[5-20]-dimethicone, and (C) balance water A foaming composition having a pH of about 2.5 to 6, preferably pH 2.9 to 4, containing

The foamable composition (a) above can stably exhibit foamability even when stored/preserved at room temperature (25±5° C.) for a long period of at least 4 weeks.

(b) (A) 50-90% by volume ethanol, (B) 0.1-10% by weight bis-PEG/PPG-[14-20]/[5-20]-dimethicone, and (C) balance water An effervescent composition of pH 4 to 4.5 containing

(c) (A) 60-80% by volume ethanol, (B) 0.1-10% by weight bis-PEG/PPG-[14-20]/[5-20]-dimethicone, and (C) balance water An effervescent composition of pH 4-5 containing

The foamable compositions (b) and (c) above can stably exhibit foamability even when stored/preserved for a long period of at least 4 weeks at a temperature of 50°C. This means that these foamable compositions are stable and foamable for at least 36 months when stored/stored at room temperature (25±5° C.). In addition, when a lower alcohol having 1 to 4 carbon atoms such as 1-propanol or 2-propanol is used as component (A) instead of ethanol, and bis-PEG/PPG-[14-20] is used as component (B). A combination of /[5-20]-dimethicone and bis-PEG-[8-20]dimethicone has similar properties.

(d) (A) 50-80% by volume ethanol, (B) 0.1-1.5% by weight bis-PEG/PPG-[14-20]/[5-20]-dimethicone, and (C) the balance of water, pH 3-5.
The effervescent composition (d) above can exert an excellent inactivating action (antiviral action) against phages, particularly MS2 phages.

(e) pH 4 containing (A) approximately 80% by volume of ethanol, (B) approximately 1% by mass of bis-PEG/PPG-[14-20]/[5-20]-dimethicone, and (C) the balance of water -4.5 foaming composition.

The effervescent composition of (e) above contains poliovirus, adenovirus, influenza virus, herpes virus, bovine viral diarrhea virus (alternative to hepatitis C virus), and caliciviridae typified by norovirus, which is a causative virus of food poisoning. It can exhibit excellent inactivating action (antiviral action) against various viruses including the virus (feline calicivirus, mouse norovirus). Bovine viral diarrhea virus is used as an alternative virus for hepatitis C virus, and feline calicivirus and mouse norovirus are used as alternative viruses for norovirus. Therefore, the effervescent composition of the present invention can exert an excellent inactivating action (antiviral action) against hepatitis C virus and norovirus.

From the above, (A) 60 to 80% by volume of ethanol, (B) 0.1 to 10% by mass of bis-PEG/PPG-[14-20]/[5-20]-dimethicone, and (C) the balance pH 4-5 effervescent composition containing water of preferably (A) 80% by volume of ethanol, (B) 1.5% by mass of bis-PEG/PPG-[14-20]/[5-20]dimethicone , and (C) the pH 4-4.5 foaming composition containing the balance water has an excellent antiviral effect and maintains foaming properties (foaming properties) even after long-term storage, It can be stably foamed when mixed with air.

The foamable composition of the present invention is an aqueous liquid composition (hydroalcoholic composition) containing the above ingredients, which when mixed with air, lasts for a variable length of time. It has a property (foamability, foamability) to form aggregates of gas cells).

In the foamable composition of the present invention, the foaming (foaming) is not limited to pressurization conditions using a propellant (e.g., hydrocarbon, fluorocarbon, gas such as compressed gas), or without using a propellant. It can be produced under no-pressure or low-pressure conditions. Specifically, for example, when a foamable composition contained in a non-gas discharge container containing no propellant is discharged from the container, air bubbles are generated by mixing with air in the container under atmospheric pressure or similar low pressure conditions. (foaming, foaming), an aggregate thereof can be obtained. However, the foamable composition of the present invention is not limited to discharge using a pressurized container (gas type discharge container) using a propellant as long as the foaming liquid can be discharged. In addition, an aerosol container can be mentioned as a representative container of the gas discharge container. The aerosol container is a container having a structure in which a target liquid composition and a liquefied gas or compressed gas are enclosed in a container having a valve, and the liquid composition inside is released by pressurizing with the force of the gas. In the present invention, since the liquid composition inside is a foaming composition, it is discharged from the container in the form of foam.

On the other hand, a non-gas discharge container is a container capable of discharging a liquid composition in the container without using a propellant. Also referred to as non-aerosol containers, in contrast to the aerosol containers described above. In the present invention, a foam-forming and foaming container (non-gas type foam-dispensing container) is preferably used which can discharge the liquid composition in the container in the form of foam. Specific examples include, but are not limited to, a pump foamer container in which foam can be discharged by pushing down the nozzle portion, and a squeeze foamer container in which foam can be discharged by manually squeezing the body of the bottle. In any of these containers, the liquid foaming composition contained in the container body is mixed with air in a gas-liquid mixing chamber provided inside the container body, and this is mixed with a net (mesh) having a predetermined hole diameter (mesh). A homogeneous foaming liquid is formed by passing through a foaming member), and the foaming liquid is discharged from the discharge port. The net (foaming member) can be provided, for example, inside the discharge cap in order to foam the liquid foaming composition immediately before pouring out of the container. For example, a cylindrical net holder may be provided between the two, and nets (foam members) may be placed in one place (single) or two places (double) at the upper and lower ends.

The mesh (pore size) of the net should be capable of forming a collection of cells that last for variable lengths of time for the foamable compositions of the present invention at atmospheric pressure or similar low pressure conditions (non-gas conditions). Although not limited, the range of 80 to 200 mesh is usually mentioned. Preferably it is 80-100 mesh. The net may be single or double, but single is preferable from the viewpoint of clogging of the pump.

Further, the non-gas type discharge container containing the foamable composition of the present invention can discharge 1 to 1.5 mL of foaming liquid (capacity in terms of liquid volume) per push or squeeze. Preferably.

The foamable composition of the present invention has an alcohol content of 50% by volume or more, preferably 60% by volume or more, with a limit of 90% by volume, relative to the total amount (100% by volume) of the foamable composition. For this reason, the alcohol content contained in the foam (cells) (gas cells surrounded by a liquid film) generated from the foamable composition is also 50% by volume or more, preferably 60% by volume, with a limit of 90% by volume. % or more. As a result, it can be used against enveloped viruses (enveloped viruses) such as influenza virus, herpes virus, and hepatitis C virus and hepatitis B virus without other additives (antibacterial agents) having additional antibacterial effects. It exhibits antiviral activity against non-enveloped viruses such as norovirus, poliovirus, adenovirus, and rotavirus (see Experimental Example 4).

Therefore, the alcoholic foam produced from the foamable composition of the present invention can be suitably used for cleaning, particularly disinfecting (antibacterial treatment, antiviral treatment), especially for disinfecting the skin including fingers and the living environment. can.

The antiviral activity of the effervescent composition of the present invention is determined according to European standards (EN: European Norm), German DVV (German Association for the Control of Virus Diseases) & RKI (Robert Koch Institute) guidelines, and US tests and materials It can be evaluated using the standard test method specified by the association (ASTM: American Society for Testing and Materials). Among these, EN 14476 and DVV & RKI guidelines are quantitative suspension methods for non-enveloped viruses, and test methods for evaluating the ability from the infectivity titer (TCID ₅₀ ) of designated indicator viruses. In addition, the in vivo standard test methods ASTM E 1838 and ASTM E 2011 are test methods for evaluating the virus inactivation effect on fingertips and the entire hand, respectively, and ASTM E 1053 is a test method for evaluating the virus inactivation effect on environmental surfaces. It is the standard test method to evaluate.

The effervescent composition targeted by the present invention may have an effect of reducing viruses in any of the above test methods. Specifically, for example, in the case of the in vivo standard test method ASTM, the logarithmic reduction value is about 2 or more, preferably about 3 or more, more preferably about 4 or more.

The foamable compositions of the present invention also exhibit very good foamability (foamability, foamability) and foam stability despite their high alcohol content. Therefore, the foaming composition can be prepared in a state of alcoholic foam (aggregate of cells) using a non-gas type discharge container or the like, and in this way drips like a solution. The foam can be spread over the entire object to be cleaned (object to be disinfected) such as hands and fingers. That is, according to the present invention, it is possible to prepare and provide an effervescent composition having stable effervescence (foamability, foamability) for cleaning or disinfecting the skin of fingers and the like.

Production method The foamable composition of the present invention is prepared by mixing the above-described (A), (B) and (C) and, if necessary, other ingredients, and adjusting the pH to a desired pH of 5 or less using (D). can be produced by adjusting the pH of Also, the foamable composition of the present invention may be initially manufactured as a concentrate containing only some of the components that may be shipped (marketable), and then, for example, at the time of use, the foamable composition may be prepared using the remaining components. The final preparation can be made as For example, the concentrate comprises (B) bis-PEG/PPG-[14-20]/[5-20]-dimethicone for foaming present in an amount of 0.1-10% by weight of the total composition. , a silicone-based surfactant, and (C) water. To this concentrated composition is then added (A) an alcohol present in an amount of 50-90% by volume per 100% by volume of the total composition; and (C) water present in an amount to bring the total composition to 100% by weight. By adding, it is possible to form the foamable composition of the present invention.

The foaming composition of the present invention thus prepared can be prepared as an actual product for actual use by placing it in a dispensing container having a device for dispensing foam. A method for producing the product includes a method of containing (filling) the foamable composition of the present invention prepared by the method described above in a discharge container having a device for dispensing foam. Here, the dispensing device includes a foaming member (predetermined (such as a net having a mesh of Mixing of the foamable composition with air in the container can be done under pressure with a propellant (gas) or under atmospheric or low pressure conditions in the absence of a propellant. It may be carried out under pressure conditions.

(II) Use of the effervescent composition of the present invention The effervescent composition of the present invention can be used as a cleaning agent, especially a disinfectant, based on its foaming properties and antiviral action described above. As used herein, "disinfect" means to prevent the growth of harmful microorganisms, kill them, or reduce the number of microorganisms. In this sense, "disinfection" is used in the sense of including "antibacterial (antiviral)" and "sterilization (virus inactivation)". Harmful microorganisms that are particularly targeted in the present invention include, as described above, influenza virus, herpes virus, enveloped viruses such as hepatitis C virus and hepatitis B virus; Non-enveloped viruses such as viruses are included.

Specifically, the effervescent composition of the present invention is a cleaning agent for cleaning and disinfecting wounds, fingers, and body surfaces of animals including humans (including skin and hair surfaces such as the head). and/or disinfectants; cleaning/disinfecting agents for cleaning and disinfecting surfaces such as blood, urine, feces, vomit, saliva or sputum; cleaning agents for cleaning and disinfecting utensils, tableware, etc. and/or Disinfectants; Disinfectants for disinfecting laundry; Disinfectants for disinfecting environmental surfaces such as furniture such as tables (desks, dining tables), chairs and sofas, and indoor equipment such as doors (disinfectants for living environments) ) can be used as Preferably, it can be used as a skin disinfectant, especially a hand disinfectant, and thus can be effectively used to prevent infectious diseases caused by viruses such as norovirus and influenza virus.

The foamable composition of the present invention is not limited to the above applications, and can be used for any application. For example, medicated foams, sunscreen foams, hand cream foams, brushless shaving foams, shower or bathing foams, dry hair shampoo foams, make-up remover foams, pain-relieving foam massaging agents, hair styling foams, and antiperspirant hair washes. Examples include foams, antiperspirant foams, hair conditioner foams, and the like.

The configuration and effects of the present invention will be described below based on experimental examples and examples. However, these experimental examples and examples are examples of the present invention, and the present invention is not limited to these experimental examples and the like.

Experimental Example 1 Evaluation of foamability of foamable composition (part 1)
Various foaming compositions (pH 2.9-7.6) containing alcohols, silicone-based surfactants, pH modifiers and water were prepared and evaluated for foaming properties.

(1) Preparation of test sample Each component (alcohol, silicone-based surfactant, pH adjuster and water) was mixed according to the formulation described in Table 1 to prepare a test sample (pH 2.9 to 7.6). Examples 1-25, Comparative Examples 1-21, Reference Examples 1-3). All of these had foamability (foamability) immediately after preparation. Next, these test samples were stored (room temperature, 50°C conditions), and how long the foamability (foamability) immediately after preparation was maintained was confirmed to evaluate the stability of foamability.

(2) Test method The test sample prepared above was placed in a 100 mL bottle (manufactured by polypropylene, Sunplatec Co., Ltd.) and stored under two conditions: room temperature (25±5°C) and 50°C. During the storage period at each temperature, the contents (foaming composition) were poured onto a laboratory table with a black surface (Yamato Scientific) using a pump foamer (discharge port mesh (single): 90 mesh) once a week. It was discharged once at a speed of about 1 second. The state of discharged foam was visually observed, and the presence or absence of foaming (with or without foaming property) was evaluated according to the following criteria.

Foaming: Dispensed in foam state, and the foam is maintained for 5 seconds or more. No foaming: Mostly discharged in liquid state.

Based on the above criteria, the presence or absence of foaming (foaming properties) of the foamable composition was checked every week during the storage period, and the stability of the foaming characteristics (foaming characteristics) of the test samples was evaluated according to the following criteria. .
[judgement]
⊚: It had foamability even after 4 weeks of storage.
◯: The foamability was lost after 3 to 4 weeks of storage.
Δ: The foamability was lost after 2 to 3 weeks of storage.
▴: The foamability was lost after 1 to 2 weeks of storage.
x: The foamability was lost in less than 1 week of storage.

(3) Test results The results are shown in Table 1.

As shown in Table 1, bis-PEG/PPG-20/20-dimethicone, bis-PEG-12 dimethicone, or bis-PEG-10 dimethicone was added to 1% by mass in an 80% by volume ethanol aqueous solution. The foaming compositions (Reference Examples 1 to 3), which were blended and adjusted to a neutral pH range (pH 6.1 to 7.6), did not lose their foaming properties even after standing at room temperature for 4 weeks. No.

However, effervescent compositions prepared by blending 1% by mass of bis-PEG-10 dimethicone or bis-PEG-12 dimethicone in 80% by volume ethanol aqueous solution and adjusting the pH to acidic (pH 3.0 to 3.7) (Comparative Examples 1 to 8) were allowed to stand at room temperature. ~3.7) lost its foamability within 3 weeks. On the other hand, effervescent compositions (Examples 1 to 6) did not lose its foamability even after 4 weeks of standing at room temperature. Also, a foaming composition prepared by blending 0.1-10% by mass of bis-PEG/PPG-20/20-dimethicone in a 50-90% by volume ethanol aqueous solution and adjusting the pH to around 4-5. Regarding (Examples 7 to 25), it was confirmed that the foamability was maintained even after 4 weeks, not only when stored at room temperature but also when stored under severe conditions of 50°C.

On the other hand, the foamable compositions of Comparative Examples 9 to 21 were prepared by blending 0.1 to 1% by mass of bis-PEG-12 dimethicone in a 50 to 80% by volume ethanol aqueous solution and adjusting the pH to around 4 to 5. As for the product, the foamability decreased or disappeared after 4 weeks. In particular, in Comparative Examples 9 to 12 in which the pH was adjusted to around 4, foamability was lost within one week after storage at 50°C.

Based on the above, when bis-PEG-12 dimethicone or bis-PEG-10 dimethicone is used as a silicone-based surfactant to be blended in an acidic high-concentration alcohol aqueous solution, the foaming property decreases and disappears over time. On the other hand, when bis-PEG/PPG-20/20-dimethicone is used instead of these, the decrease or disappearance of foaming property over time is suppressed, and foaming property ( It was found that the foamability) was maintained.

Experimental Example 2 Evaluation of foamability of foamable composition (part 2)
Foaming Compositions (around pH 4) Prepared Using Surfactants Containing Bis-PEG/PPG-20/20-Dimethicone and Bis-PEG-12 Dimethicone as Silicone-Based Surfactants (Examples 26-29) and 1-propanol or 2-propanol as the alcohol was used to evaluate foaming stability in the same manner as in Experimental Example 1 for foaming compositions (about pH 4) (Examples 30-33).

(1) Preparation of test sample and test method Each component (alcohol, silicone-based surfactant, pH adjuster and water) was mixed according to the formulation described in Table 2 to prepare a test sample (about pH 4). (Examples 26-33). All of these had foamability (foamability) immediately after preparation. Next, these test samples were stored (room temperature, 50° C. conditions), and how long the above-mentioned foamability (foamability) was maintained was confirmed to evaluate foamability stability.

The test method was performed according to the method described in Experimental Example 1, and the evaluation was performed according to the criteria described in Experimental Example 1.

(2) Test results The results are shown in Table 2.

As shown in Table 2, 0.5 wt% or 1 wt% concentration of bis-PEG/PPG-20/20-dimethicone and 1 wt% concentration of bis-PEG- The foaming compositions containing 12 dimethicone and adjusted to an acidic pH (pH 4) (Examples 26-29) did not lose their foaming properties when stored at room temperature and 50°C for 4 weeks.

In addition, foaming compositions (Examples 30 to 33) whose pH was adjusted to acidic (pH 4) using 1-propanol or 2-propanol instead of ethanol as alcohol were also stored at room temperature and 50° C. for 4 weeks. However, it did not lose its foamability.

Based on the above, bis-PEG-[ 10-20] It was found that even when used in combination with dimethicone, the decrease or disappearance of foaming properties over time was suppressed, and foaming properties (foaming properties) were maintained for a long period of time even when stored under acidic conditions (pH 4 or less). did. It was also confirmed that the foamability and foaming stability of the foamable composition of the present invention can be similarly obtained not only with ethanol but also with lower alcohols such as 1-propanol and 2-propanol. .

Experimental Example 3 Evaluation of antiviral action of effervescent composition (Part 1)
The effervescent composition prepared in Experimental Example 1 (Examples 1, 2, 4, 6-21, 23, Reference Example 1-2) was used as a test sample, and its phage-inactivating effect (antiviral effect) was evaluated using MS2 phage. was evaluated using

(1) Phage used and its preparation
The MS2 phage is highly resistant to drugs and can be tested relatively easily, so it is often used as an alternative virus for efficacy evaluation against various viruses (see Non-Patent Documents 7 and 8). Therefore, in this experiment, MS2 phage was used to evaluate the phage inactivation effect (antiviral effect) of the test sample.

[Phage and host bacteria used]
Escherichia coli NBRC13965 (host fungus) and Escherichia coli phage MS2 NBRC20015 (MS2 phage) using this as a host fungus were used in the test.

[Preparation of host bacterial solution]
The host strain was inoculated into a trypto-soy broth medium (TSB medium) and cultured overnight at 37°C with shaking.

[Preparation of phage solution]
After culturing the host fungus for 3 to 4 hours, the MS2 phage was added and cultured overnight to infect the host fungus with the phage. The supernatant obtained by centrifugation was used for the test as a phage liquid.

(2) Experimental method To 0.9 mL of the test sample, 0.1 mL of the phage solution prepared above was added and allowed to act at 25°C for 5 minutes. After action, 0.1 mL thereof was added to 0.9 mL of neutralizer solution (0.1 mM phosphate buffer) to stop the action and serially diluted with sterile saline. 0.1 mL of this diluted solution and 0.1 mL of the host bacteria solution prepared above are each mixed with 4 mL of 10 mM magnesium sulfate-containing trypto-soy broth soft agar medium (TSB medium with 0.75% agar powder added). ), and after stirring so as not to foam, the mixture was overlaid on a trypto-soy bouillon agar medium prepared in advance, allowed to solidify, and cultured overnight at 37°C. After culturing, plaque numbers (pfu/mL) were determined (phage plaque numbers after treatment).

As a control, a phage solution prepared to give a plaque count of about 10 ⁸ pfu/mL was used (untreated phage plaque count).

(3) Experimental results The results are shown in Table 3. In Table 3, the "logarithmic reduction value" was calculated by "(the logarithmic value of the plaque number of untreated phage) - (the logarithmic value of the plaque number of phage after treatment)".

As shown in Table 3, Examples 1, 4, 15 and 16 reduced MS2 phage by 3 Log or more (reduction of 99.9% or more), and Examples 2, 6 to 14, 17 to 21 and 23 reduced MS2 phage by 2 Log or more. It was confirmed to reduce (more than 99% reduction). When the ethanol concentration was increased to 40% by volume, there was a tendency for the MS2 phage reduction rate (phage inactivation effect) to decrease even in acidic conditions (logarithmic reduction value: 0.91, reduction rate: 87.7%).

On the other hand, in the case of neutral foaming compositions such as Reference Examples 1 and 2, the MS2 phage decreased by less than 1 Log (90% or less), and the acidic foaming compositions (Examples 1, 2, 4 , 6-21, 23), the phage inactivation effect was clearly lower.

From the above, in order to exhibit a high phage inactivation effect that reduces MS2 phage by 2 Logs or more (reduction of 99% or more), the ethanol concentration of the foamable composition should be 50% by volume or more, particularly 50 to 80% by volume. In addition, it was found that the pH should be acidic (pH 5 or less, preferably pH 4 or less).

Experimental Example 4 Evaluation of antiviral action of effervescent composition (Part 2)
Using the effervescent compositions prepared in Experimental Example 1 (Examples 7, 11, and 19) as test samples, their inactivating action (antiviral action) against various viruses was evaluated.

(1) Preparation of target virus and virus solution
[Target virus]
Feline calicivirus F9 strain (hereinafter referred to as "FCV")
Mouse norovirus S7 strain (hereinafter referred to as "MNV")
Poliovirus type 1 (hereinafter referred to as "polio")
Adenovirus type 5 (hereinafter referred to as "adeno")
Influenza virus A (H1N1) type (hereinafter referred to as "influenza")
Bovine viral diarrhea virus (hereinafter referred to as "BVDV")
Herpes virus type 1 (hereinafter referred to as "herpes")
Both the feline calicivirus F9 strain (FCV) and the mouse norovirus S7 strain (MNV) are substitute viruses for norovirus (Non-Patent Documents 2, 9 and 10). Also, bovine viral diarrhea virus is an alternative virus to hepatitis C virus.

[Cells used]
FCV: CRFK cells
MNV: RAW264 cells Polio: Vero cells Adeno: Hela cells Influenza: MDCK cells
BVDV: MDBK cells Herpes: Vero cells.

[Medium used for culture]
Dulbecco's modified Eagle's medium (DMEM) was used for FCV, adeno and influenza, and Eagle's minimum essential medium (MEM) was used for MNV, polio, BVDV and herpes. The sera added to the medium were newborn calf serum (NCS) for FCV and influenza; fetal bovine serum (FBS) for MNV, polio, adeno and herpes; and horse serum (HS) for BVDV.

[Method for preparing virus solution]
The above-mentioned various viruses were added to each cell prepared in advance, and after culturing for a certain period of time, the culture solution was freeze-thawed, and the supernatant obtained by centrifugation was used as the virus solution.

(2) Experimental method 0.1 mL of the virus solution was added to 0.9 mL of the test sample, and allowed to act at 20°C for 1 minute. After the action, the reaction was terminated by 10-fold dilution with a 10% serum-containing medium, followed by serial dilution. 0.1 mL of this diluted solution was placed in a plate on which cells had been cultured in advance, and FCV, MNV, polio, adeno and BVDV were incubated at 37°C and 5% CO ₂ for 1 hour, and influenza and herpes were incubated at 34°C and 5% CO 2 for 5 hours. After culturing for 1 hour under % _CO2 conditions and replacing with fresh medium, FCV, MNV, polio and adeno were cultured at 37°C under 5% _CO2 conditions for 3-4 days, and BVDV under the same conditions for 5 days. . Influenza and herpes were cultured under conditions of 34°C and 5% CO ₂ for 5 days. After culturing, the cytopathic effect was confirmed by microscopic observation, and the virus infectivity titer (TCID ₅₀ : 1 TCID ₅₀ is the amount of virus that infects cultured cells with the virus and infects 50% of them) by the Behrens-Kaerber method. , the Log ₁₀ reduction was calculated.

(3) Experimental Results Table 4 shows the results.

Examples 7, 11 and 19 were evaluated for effectiveness against FCV (feline calicivirus), MNV (murine norovirus), poliovirus, adenovirus, influenza, BVDV, and herpes. No infection was observed, indicating high efficacy (virus inactivation).

From the above, it can be concluded that an effervescent composition having an ethanol concentration of 60% by volume or more and an acidic pH (pH 5 or less, preferably pH 4 or less) can inactivate various viruses, including enveloped viruses and non-enveloped viruses. It was confirmed that it exerts an action (antiviral action).

Claims (5)

Bis -PEG/PPG-[14-20]/[5-20]-dimethicone is blended in a pH 2-5 composition containing alcohol and water so that the amount is 0.1-10% by mass, A method of imparting a function of maintaining foamability to the composition having a pH of 2 to 5 by preparing an alcohol-containing foamable composition of 50 to 90% by volume. The method of claim 1, further comprising bis-PEG-[8-20]dimethicone. 3. The method according to claim 1 or 2, wherein the pH 2-5 composition is adjusted with a pH adjuster selected from the group consisting of acids and salts thereof. The method according to any one of claims 1 to 3, wherein the pH 2-5 composition is a disinfecting composition having an antiviral effect. The alcohol-containing foaming composition is prepared as an actual product by being placed in a dispensing container having a device for dispensing foam,
1- wherein the dispensing device comprises a foam member configured to mix air with the composition to form a foam as the composition is dispensed from the dispensing container. 5. The method according to any one of 4.