JP7345886B2 - Alcohol-containing composition - Google Patents

Description

The present invention relates to a composition containing a lower alcohol and a nonionic surfactant.

Every year, infectious diseases caused by various viruses such as influenza virus and norovirus have become a problem. These infectious diseases spread through droplet infection and contact infection. Disinfecting hands with disinfectant is important as a measure to prevent the spread of infection, but most of these are in liquid form. In order to uniformly apply disinfectant to hands and fingers, a large amount is often sprayed or applied. Furthermore, when using a disinfectant, a phenomenon occurs in which the solution runs down from the hands and fingers (dripping), which results in a portion of the solution being wasted and staining clothes, floors, etc. Furthermore, a gel composition of a disinfectant has also been developed (for example, Patent Document 1). Although gel-like compositions are less prone to dripping, there are problems in that they remain sticky after application and are difficult to apply uniformly. In addition, in recent years, foam-like compositions have been developed for the purpose of uniform application, but since the lower alcohol, which is an active ingredient, has an antifoaming effect, it has good foam retention even though it contains lower alcohol. There is a need to develop a composition that can be applied uniformly.

Japanese Unexamined Patent Publication No. 11-199476

An object of the present invention is to provide a composition that has good foam retention and an excellent feel when used, even though it contains a lower alcohol.

In order to solve the above problems, the present applicant has conducted intensive studies on various substances, and surprisingly, the composition of the present invention containing a lower alcohol and a nonionic surfactant has been found to be able to remove foam from a foamer container. It was found that the foam had good foam retention, no dripping occurred, and had an excellent feeling of use. In addition, in order to solve the above problems, the present applicant has conducted intensive studies on various substances, and surprisingly, the composition of the present invention, despite containing a high concentration of lower alcohol, When the foam was discharged from a foamer container, it was found that the foam had good retention, no dripping occurred, and had an excellent feeling of use. The present invention has been completed based on this knowledge.

The outline of the present invention is as follows.
<1> A composition that is used by discharging it in the form of a foam from a foamer container, containing a lower alcohol and a nonionic surfactant, and characterized in that the content of the lower alcohol is 30 to 60% by mass. composition.
<2> The nonionic surfactant is polyoxyethylene alkyl ether, polyoxyethylene polyoxypropylene alkyl ether, polyoxyalkylene sorbitan fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, glycerin fatty acid ester, polyether-modified silicone The composition according to <1>, which is at least one selected from the group consisting of:
<3> The composition according to any one of <1> or <2>, which contains an acid or a salt thereof.
<4> The composition according to any one of <1> to <3>, further comprising a cationic surfactant.
<5> The composition according to any one of <1> to <4>, further containing water.
<6> The composition according to any one of <1> to <5>, further containing a humectant.
<7> The composition according to any one of <1> to <6>, which is used for sterilizing, disinfecting, and disinfecting the skin.
<8> The composition according to any one of <1> to <6>, which is used for sterilizing, sterilizing, and disinfecting hard surfaces.

According to the present invention, it is possible to obtain a composition that has good foam retention, does not cause dripping, and has an excellent feeling of use. In particular, according to the present invention, it is possible to obtain a composition that has good foam retention, does not cause dripping, and has an excellent feel when used, despite containing a high concentration of lower alcohol. Furthermore, since the composition of the present invention can be used not only for sterilizing, sterilizing, and disinfecting the skin of hands and fingers, but also for hard surfaces, it can be expected to be effective in preventing the spread of contact infection.

The present invention will be explained in detail below.

[1. Lower alcohol]
The present invention uses a lower alcohol as an essential component. The lower alcohol that can be used in the present invention is not particularly limited as long as it is normally used as an external skin preparation, and examples thereof include ethanol and isopropanol. In the present invention, ethanol is preferred from the viewpoint of both the feeling of use such as foam retention, ease of application, and uniformity during use, and the sterilization, sterilization, and disinfection effects.

The lower alcohols used in the present invention may be used alone or in combination of two or more. The content of the lower alcohol to be blended into the composition of the present invention is determined from the viewpoint of both feeling of use such as foam retention, ease of application, and uniformity during use, and sterilization, sterilization, and disinfection effects, for example: It is selected in a range of 30% by mass, preferably 35% by mass or more, more preferably 40% by mass or more. Further, from the same viewpoint, the content of the disinfectant is selected within a range of, for example, 95% by mass or less, preferably 60% by mass or less, more preferably 55% by mass or less, particularly preferably 50% by mass or less. However, if two or more kinds of lower alcohols are contained, the amount is the total amount.

[2. Nonionic surfactant]
The composition of the present invention is characterized by containing a nonionic surfactant together with a lower alcohol. By blending a nonionic surfactant with a lower alcohol, it is possible to achieve both a feeling of use such as foam retention, ease of application, and uniformity during use, as well as sterilizing, sterilizing, and disinfecting effects.

Examples of nonionic surfactants include glycerin fatty acid esters such as glyceryl laurate and glyceryl monostearate; sorbitan fatty acid esters; pentaethylene glycol monododecyl ether, octaethylene glycol monododecyl ether, polyoxyethylene nonylphenyl ether, and Oxyethylene oleyl ether, polyoxyethylene stearyl ether, polyoxyethylene lauryl ether, polyoxyethylene cetyl ether, polyoxyethylene behenyl ether, polyoxyethylene 2-hexyldecyl ether, polyoxyethylene octyl dodecyl ether, polyoxyethylene cholesteryl ether polyoxyethylene alkylaryl ether or polyoxyethylene alkyl ether such as; polyoxyethylene polyoxypropylene alkyl ether such as polyoxyethylene polyoxypropylene decyl tetradecyl ether, polyoxyethylene polyoxypropylene cetyl ether; polyoxyethylene alkylphenyl Alkylphenol alkylates such as ether, octylphenol ethoxylate, nonylphenol ethoxylate; polyoxyalkylene glycols such as polyoxyethylene polyoxypropylene glycol; polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitan monostearate, polyoxyethylene hexitane fatty acid ester, sorbitan fatty acid ester Polyoxyalkylene sorbitan fatty acid ester such as polyethylene glycol; polyoxyalkylene fatty acid ester such as polyethylene glycol monolaurate, polyethylene glycol monostearate, polyethylene glycol monooleate; polyglyceryl monolaurate, polyglyceryl monostearate Polyglycerin fatty acid esters such as; sucrose fatty acid esters such as sucrose stearate; glycerin fatty acid esters such as glyceryl caprylate; alkanolamides such as lauric acid diethanolamide, oleic acid diethanolamide, stearic acid diethanolamide, and cocamide DEA; octyl glucoside , alkyl glucosides such as decyl glucoside and lauryl glucoside; oxyethylene/oxypropylene block copolymers; fluorosurfactants; polyglyceryl-modified silicones, diglycel-modified silicones, glyceryl-modified silicones, sugar-modified silicones, polyether-modified silicones (PEG/PPG- Silicone surfactants such as 25/25 dimethicone, bis(PEG/PPG-20/20) dimethicone, bis PEG-18 methyl ether dimethylsilane, PEG-12 dimethicone), carboxylic acid-modified silicone; decyl glucoside, lauryl glycoside, etc. Polyoxyethylene alkyl ether, polyoxyethylene polyamide, Oxypropylene alkyl ether, polyoxyalkylene sorbitan fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, glycerin fatty acid ester, and polyether-modified silicone are preferred, and polyoxyethylene alkyl ether is particularly preferred.

When using a surfactant in the present invention, only one type of surfactant may be used, or two or more types may be used. When a surfactant is added to the composition of the present invention, the content should be within a range that provides both a feeling of use such as foam retention, ease of application, and uniformity during use, as well as sterilizing, sterilizing, and disinfecting effects. If so, there is no particular restriction, and the content can be set as appropriate. For example, it is selected in the range of 0.0001% by mass, preferably 0.001% by mass or more, more preferably 0.005% by mass or more. Further, from the same viewpoint, the content of the surfactant is selected within a range of, for example, 15% by mass or less, preferably 10% by mass or less, more preferably 8% by mass or less, particularly preferably 5% by mass or less. However, if two or more types of surfactants are contained, the amount is the total amount. In the present invention, commercially available products may be used, or products manufactured by methods known in the art may also be used.

[3. acid or its salt]
The composition of the present invention preferably contains an acid or a salt thereof. By blending an acid or a salt thereof, the stability of the composition is improved, and it is also possible to improve the stability of the foam. The acid or salt thereof that can be used in the composition of the present invention is not particularly limited, and acids or salts thereof that are commonly used for pH adjustment can be used. Specifically, organic acids or their salts, inorganic acids or their salts are mentioned; inorganic acids or their salts such as hydrochloric acid, sulfuric acid, phosphoric acid, and nitric acid; formic acid, acetic acid, propionic acid, sulfamic acid, gluconic acid, and ascorbic acid. Preferred examples include ferulic acid, succinic acid, phytic acid, lactic acid, citric acid, malic acid, maleic acid, malonic acid, caprylic acid, adipic acid, fumaric acid, etidronic acid, edetic acid, or salts thereof. is hydrochloric acid, sulfuric acid, lactic acid, gluconic acid, succinic acid, citric acid, malic acid, etidronic acid, edetic acid, phosphoric acid, or salts thereof, and more preferably hydrochloric acid, sulfuric acid, lactic acid, gluconic acid, citric acid, apple acids, etidronic acid, edetic acid and phosphoric acid or salts thereof.

When using acids or salts thereof in the present invention, only one type of acid or salt thereof may be used, or two or more types thereof may be used. When an acid or a salt thereof is added to the composition of the present invention, the content thereof is such that it can provide both a feeling of use such as foam retention, ease of application, and uniformity during use, as well as sterilizing, sterilizing, and disinfecting effects. There is no particular restriction as long as it is within the range, and the content can be set as appropriate. For example, it is selected in the range of 0.0001% by mass, preferably 0.0005% by mass or more, more preferably 0.001% by mass or more. Further, from the same viewpoint, the content of the acid or its salt is selected within a range of, for example, 20% by mass or less, preferably 15% by mass or less, and more preferably 10% by mass or less. However, if two or more types of acids or salts thereof are contained, the amount is the total amount. In the present invention, commercially available products may be used, or products manufactured by methods known in the art may also be used.

[4. Cationic surfactant]
The composition of the present invention preferably contains a cationic surfactant. Examples of the cationic surfactant include oleylamine, stearylamine, tetradecylamine, 1-hexenylamine, 1-dodecenylamine, 9,12-octadecadienylamine (linolamine), 9,12,15-octadecadienylamine, Aliphatic amines such as alkylamines, such as trienylamine and linoleylamine; tetramethylammonium chloride, tetramethylammonium hydroxide, tetrabutylammonium chloride, alkyltrimethylammonium chloride, octyltrimethylammonium chloride, decyltrimethylammonium chloride, chloride Dodecyltrimethylammonium, Tetradecyltrimethylammonium chloride, Cetyltrimethylammonium chloride, Stearyltrimethylammonium chloride, Alkyltrimethylammonium bromide, Hexadecyltrimethylammonium bromide, Benzyltrimethylammonium chloride, Benzyltriethylammonium chloride, Benzalkonium chloride (Dodecyl chloride) quaternary ammonium salts such as dimethylbenzylammonium), benzalkonium bromide, benzethonium chloride, didecyldimethylammonium chloride, distearyldimethylammonium chloride; alkylpyridinium salts such as butylpyridinium chloride, dodecylpyridinium chloride, cetylpyridinium chloride; Aliphatic amine salts such as monomethylamine hydrochloride, dimethylamine hydrochloride, and trimethylamine hydrochloride; and the like. In the present invention, quaternary ammonium salts are preferable, and benzalkonium chloride is preferable from the viewpoint of both feeling of use such as foam retention, ease of application, and uniformity during use, and sterilization, sterilization, and disinfection effects. Particularly preferred.

[5. water]
The composition of the present invention can contain water in addition to the lower alcohol and nonionic surfactant. When water is added to the composition of the present invention, the content should be within a range that provides both a feeling of use such as foam retention, ease of application, and uniformity during use, as well as sterilizing, sterilizing, and disinfecting effects. There are no particular restrictions, and the content can be set as appropriate. For example, it is selected in the range of 1% by mass, preferably 10% by mass or more, more preferably 20% by mass or more. Further, from the same viewpoint, the water content is selected within a range of, for example, 80% by mass or less, preferably 75% by mass or less, and more preferably 70% by mass or less.

[6. Moisturizer]
The composition of the invention can contain a humectant in addition to the lower alcohol. Humectants that can be used include, for example, glycerin, 1,3-butylene glycol, propylene glycol, 3-methyl-1,3-butanediol, 1,3-propanediol, 2-methyl-1,3-propanediol, Polyols and their polymers such as trimethylolpropane, pentaerythritol, hexylene glycol, diglycerin, polyglycerin, diethylene glycol, polyethylene glycol, dipropylene glycol, polypropylene glycol, ethylene glycol/propylene glycol copolymer; diethylene glycol monoethyl ether Glycol alkyl ethers such as (ethoxydiglycol), ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol dibutyl ether; (eicosandioic acid/tetradecanedioic acid) polyglyceryl-10, tetradecanedioic acid polyglyceryl-10, cyclohexanedicarboxylic acid Water-soluble esters such as bisethoxydiglycol; sugar alcohols such as sorbitol, xylitol, erythritol, mannitol, maltitol; glucose, fructose, galactose, mannose, threose, xylose, arabinose, fucose, ribose, deoxyribose, maltose, Trehalose, lactose, raffinose, gluconic acid, glucuronic acid, cyclodextrins (α-, β-, γ-cyclodextrin, and modified cyclodextrins such as maltosylation and hydroxyalkylation), β-glucan, chitin, chitosan, Saccharides and their derivatives such as heparin and derivatives, pectin, arabinogalactan, dextrin, dextran, glycogen, ethyl glucoside, glucosylethyl methacrylate polymers or copolymers; hyaluronic acid, sodium hyaluronate; sodium chondroitin sulfate; mucoitin sulfate , caronine sulfate, keratosulfate, dermatan sulfate; white fungus extract, white fungus polysaccharide; fructans such as inulin and levan; fucoidan; tuberose polysaccharide, naturally derived polysaccharides; organic acids such as citric acid, tartaric acid, lactic acid, and their salts ; Urea; 2-pyrrolidone-5-carboxylic acid and its salts such as sodium; betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, tyrosine, β-alanine, threonine, glutamic acid , glutamine, asparagine, aspartic acid, cysteine, cystine, methionine, leucine, isoleucine, valine, tryptophan, histidine, taurine, and other amino acids and their salts; collagen, fish-derived collagen, atelocollagen, gelatin, elastin, collagen-degrading peptide, hydration Degraded collagen, hydroxypropylammonium chloride hydrolyzed collagen, elastin-degraded peptide, keratin-degraded peptide, hydrolyzed keratin, conchiolin-degraded peptide, hydrolyzed conchiolin, silk proteolytic peptide, hydrolyzed silk, sodium lauroyl hydrolyzed silk, soybean proteolytic peptide , protein peptides such as wheat proteolytic peptide, hydrolyzed wheat protein, caseinolytic peptide, acylated peptide, and derivatives thereof; acylated peptides such as palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide; silylated peptides; Lactic acid bacteria culture solution, yeast extract, eggshell membrane protein, bovine submandibular gland mucin, hypotaurine, sesame lignan glycoside, glutathione, albumin, whey; choline chloride, phosphorylcholine; placenta extract, aerastin, collagen, aloe extract, Hamamelis Animal and plant extract ingredients such as water, loofah water, chamomilla extract, licorice extract, comfrey extract, silk extract, rose thorn extract, yarrow extract, eucalyptus extract, melilot extract; natural ceramides (types 1, 2, 3, 4) , 5, 6), ceramides such as hydroxyceramide, pseudoceramide, glycosphingolipid, ceramide, and sugar ceramide-containing extract. Among these, polyols, polymers thereof, and sugar alcohols are preferred, and glycerin, 1,3-butylene glycol, propylene glycol, 3-methyl-1,3-butanediol, 1,3-propanediol, and diglycerin are more preferred. preferable.

When using a humectant in the present invention, only one type of humectant may be used, or two or more types may be used. When a moisturizer is added to the composition of the present invention, the content should be within a range that provides both a feeling of use such as foam retention, ease of application, and uniformity during use, as well as sterilizing, sterilizing, and disinfecting effects. If so, there is no particular restriction, and the content can be set as appropriate. For example, it is selected in the range of 0.01% by mass, preferably 0.05% by mass or more, more preferably 0.1% by mass or more. Further, from the same viewpoint, the content of the humectant is selected within a range of, for example, 20% by mass or less, preferably 15% by mass or less, more preferably 8% by mass or less, particularly preferably 10% by mass or less. However, if two or more types of humectants are contained, the amount is the total amount. In the present invention, commercially available products may be used, or products manufactured by methods known in the art may also be used.

[6. Former container]
The composition of the present invention is used by being filled into a foamer container, and is characterized in that it is used by being discharged from the foamer container in the form of a foam. A foamer container is a container that can form foam by mixing a composition and gas at a certain ratio during discharge. Examples of gases that are mixed with the composition and form bubbles during discharge include air; liquefied gases such as liquefied petroleum gas (LPG) and dimethyl ether; compressed gases such as carbon dioxide, nitrogen, and nitrous oxide; and the like. . The dispensing method is not particularly limited and can be selected as appropriate depending on the structure of the container.For example, pressure is applied by pressing the nozzle part, gripping the bottle body, etc.; Sensing the object with an infrared sensor etc. and discharging; Spraying Examples of methods include mixing the composition in the container and the atomizing gas and discharging the mixture by pressing or squeezing a button. The discharge amount can be changed as appropriate depending on the discharge method. In the present invention, from the viewpoint of sterilization, sterilization, and disinfection, it is preferable to use a former container having a structure in which the nozzle part is pressed, or a former container having a structure in which the object is sensed by a sensor such as infrared rays and discharged. Further, from an economical point of view, it is preferable that the foamer container has a structure that can be used repeatedly by removing the lid and filling the container with the composition.

[7. Composition of the present invention]
The composition of the present invention may contain only a lower alcohol and a nonionic surfactant, and in addition to the lower alcohol and nonionic surfactant, any one of an acid, a cationic surfactant, water, and a humectant It may contain one or more kinds, or it may contain other components in addition to these. Other ingredients include, for example, thickeners, plant-derived ingredients, animal-derived ingredients, and fragrances. In addition, the composition of the present invention can be filled into a foamer and discharged in the foamed state in the amount necessary for sterilization, sterilization, or disinfection, and the discharged composition can be applied to the area to be sterilized, sterilized, or disinfected. can be used.

The foam formed by the composition of the present invention easily adapts to the area where it is applied and has excellent foam retention. In the present invention, the term "foam retention" refers to the fact that the foam formed during discharge from the foamer container is maintained for a certain period of time or longer without disappearing. The maintenance time of the formed bubbles is preferably 5 seconds or more, more preferably 10 seconds or more, and particularly preferably 15 seconds or more from the viewpoint of uniformly applying the discharged composition.

Further, the composition of the present invention is preferably adjusted to be acidic. Specifically, the composition of the present invention preferably has a pH of 6 or less, more preferably a pH of 5.5 or less, and particularly preferably a pH of 5 or less. The lower limit of pH is not limited and is usually pH 1 or higher, preferably pH 2 or higher, and more preferably pH 3 or higher.

[8. Use of the composition of the present invention]
The composition of the present invention can be used as a sterilizing, sterilizing, and disinfecting composition. Sterilization refers to the effect of killing bacteria, sterilization refers to the effect of reducing bacteria, and disinfection refers to the effect of killing (inactivating) or removing pathogenic microorganisms such as viruses. To make something harmless (to make it non-toxic). The composition of the present invention can be labeled as having sterilizing, sterilizing, and disinfecting functions, and these functions can be stated on the main body, packaging, instructions, and advertising materials of the product according to the present invention. . The composition of the present invention can be used for the purpose of providing sterilizing, sterilizing, and disinfecting effects, and includes, for example, skin and hard surfaces. Specific examples of the skin include hands, wrists, fingers, and nails. Examples of hard surfaces include information devices such as computers, mice, keyboards, smartphones, tablets, telephones, printers, copiers, multifunction devices, calculators, audio equipment, and video equipment; tableware such as cups, plates, spoons, forks, and chopsticks. ; Stationery such as pens and rulers; Tableware such as cups and plates; Furniture such as desks, chairs, tables, counters, lockers, and cabinets; Fittings such as doors, doorknobs, and windows; Equipment such as remote controls; Refrigerators, microwave ovens, etc. Household appliances; switches; faucets; toilets; handrails; floors; and other items with hard surfaces that come into contact with people's skin in daily life.

When the composition of the present invention is used as a composition for sterilization, sterilization, or disinfection, the objects to be sterilized, sterilized, or disinfected are not particularly limited, but include, for example, influenza viruses (e.g., type A, type B, etc.), rubella virus, Ebola virus, coronavirus, measles virus, varicella/shingles virus, herpes virus, mumps virus, arbovirus, respiratory syncytial virus, SARS virus, hepatitis virus (e.g., hepatitis B virus, hepatitis C virus, etc.), yellow fever virus , AIDS virus, rabies virus, hantavirus, dengue virus, Nipah virus, lyssavirus, and other enveloped viruses; adenovirus, norovirus, rotavirus, human papillomavirus, poliovirus, enterovirus, coxsackie virus, human parvovirus, cerebrocardial virus Non-enveloped viruses (viruses that do not have envelopes) such as inflammatory viruses and rhinoviruses; Vibrio cholerae, Salmonella enterica, Escherichia coli, Legionella bacteria, Bacillus anthrax, Helicobacter pylori, Listeria monocytogenes, Mycobacterium tuberculosis, non-tuberculous mycobacteria, grapevine Coccus, Streptococcus, Streptococcus pneumoniae, Neisseria meningitidis, Klebsiella pneumoniae, Serratia monocytogenes, Diphtheria monocytogenes, Brucella monocytogenes, Bartonella hensele, Erysiperoshrix rhusiopathiae, Actinomycetes, Lyme disease, Clostridium perfringens, Shigella, Yersinia pestis Bacteria associated with infectious diseases such as Clostridium tetani and Enterobacter; Fungi associated with infectious diseases such as Candida, Aspergillus, Cryptococcus, Blastomyces, Coccidioides, Histoplasma, Paracoccidioides, and Sporothrix. Among these, enveloped viruses are preferred, and influenza viruses, herpes viruses, coronaviruses, and the like are more preferred.

Hereinafter, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited to these Examples, and the present invention can take various embodiments as long as the problems of the present invention can be solved. .

Examples 1-2: Evaluation of antiviral effect A composition containing a lower alcohol and a nonionic surfactant was produced according to the composition shown in Table 1 below. The blending amounts listed in the table indicate mass %. Specifically, each component was uniformly stirred at room temperature to obtain a composition. The antiviral effect of the obtained composition was evaluated.

<Antiviral effect>
Using the SARS-CoV-2 Inhibitor Screening Kit (Acro Biosystems), we investigated the binding inhibitory activity between the spike (S) protein of the novel coronavirus SARS-CoV-2 and the angiotensin-converting enzyme 2 (ACE2) protein localized in the human cell membrane. evaluated. Specifically, the evaluation was performed according to the following procedure. First, a buffer was prepared as follows. Coating buffer: 15mM sodium carbonate (Nacalai Tesque) and 35mM sodium bicarbonate (SIGMA) were dissolved in ultrapure water (pH 9.6). Washing buffer: Phosphate Buffered Saline with Tween 20 Tablets (Takara Bio) 1 tablet was dissolved in 1 L of ultrapure water. Blocking buffer: Blocker (TM) BSA (10X) in PBS (Thermo Scientific (TM)) was diluted 5 times with washing buffer. Sample buffer: Blocking buffer was diluted 4 times with wash buffer. Next, 100 μl of SARS-CoV-2 S protein RBD working solution (0.5 μg/mL) was added to each well except the blank and incubated overnight at 4°C to coat the S protein on the wells. The wells were washed, 300 μl of blocking buffer was added, and the wells were incubated at 37° C. for 1.5 hours for blocking. Wash the wells, add 50 μl of biotinylated human ACE2 working solution (0.12 μg/mL) to each well except the blank (add 50 μl of sample buffer to the blank well), and add the test substance diluted 2 times with sample buffer. 50 μl was added to each well other than the positive control and blank (50 μl of sample buffer was added to the positive control and blank wells), and incubated at 37°C for 1 hour to allow binding of S protein and ACE2. The wells were washed, 100 μl of streptavidin-HRP working solution (0.1 μg/mL) was added, and biotinylated human ACE2 was labeled by incubating at 37°C for 1 hour in the dark. The wells were washed, 100 μl of TMB solution (Fuji Film Wako Pure Chemical Industries, Ltd.) was added to each well, and incubated at 37°C for 20 minutes in the dark to react with HRP. The reaction was stopped by adding 100 μl of 0.3M sulfuric acid, and the absorbance at 450 nm was measured using a plate reader (SpectraMax i3x, Molecular Devices). The inhibition rate was calculated using the following formula.
Inhibition rate (%) = 100-{(Date sample-Date blank)/(Date positive control-Date blank)}×100
(Date sample: Absorbance of S protein and ACE2 reacted with test substance
Date blank: Absorbance obtained by the same operation with the test substance but without adding S protein
Date positive control: Absorbance of S protein and ACE2 reacted without test substance
Date blank: Absorbance obtained by performing the same procedure without the test substance and without adding S protein)
The results are shown in Table 1.

Evaluation of antiviral effect shows the extent to which pathogenic viruses can be rendered nontoxic, so it can be used as an evaluation of disinfection effect. Viral infection occurs when the virus binds to the host cell surface and invades into the cell, so components that inhibit the binding between the virus and the host cell are thought to have a suppressive effect on viral infection. The novel coronavirus SARS-CoV-2 is known to bind to human cells through a specific bond between the S protein present in the virus envelope and the ACE2 protein present on the human cell membrane. In this study, the antiviral effect of the composition was evaluated by an interaction test between S protein and ACE2 protein using the new coronavirus SARS-CoV-2 as a model. From the test results, composition examples 1 and 2 of the present invention in which ethanol is 30% to 50% by mass and a nonionic surfactant are blended, comparative example 1 in which only a nonionic surfactant is blended, and ethanol It can be seen that it exhibits superior antiviral activity compared to Comparative Example 2 in which the amount is 20% by mass. This shows that the composition of the present invention containing 30 to 60% by mass of lower alcohol and a nonionic surfactant exhibits excellent antiviral action (ie, disinfecting action).

Examples 3 to 10: Evaluation of feeling in use Compositions containing a lower alcohol and a nonionic surfactant were produced according to the compositions shown in Table 2 below. The blending amounts listed in the table indicate mass %. Specifically, each component was uniformly stirred at room temperature to obtain a composition. The resulting composition was filled into a foamer container, and the quality of foam upon discharge and the feeling of use upon discharge of foam were evaluated in the following manner. The results are shown in Table 2.
<Foam quality>
・〇: Forms foam with shape retention (held for 15 seconds or more)
・△: Bubbles are formed, but the bubbles do not have sufficient shape retention (5 to 14 seconds)
・×: No bubbles / Even if bubbles are formed, they disappear immediately <Feeling when dispensing bubbles>
・〇: Not sticky when applied to the skin ・△: Slightly sticky when applied to the skin
・×: Sticky when applied to the skin

From Table 2, Comparative Examples 3 to 5 did not produce bubbles, and the feeling of use when discharging bubbles could not be evaluated. On the other hand, Examples 3 to 10 containing 30 to 40% by mass of ethanol and a nonionic surfactant were able to form foam with better retention compared to Comparative Examples 3 to 5, had almost no stickiness, and had a feeling of use. It was excellent. From this, it can be seen that the composition of the present invention containing 30 to 60% by mass of a lower alcohol and a nonionic surfactant is a composition with excellent foam quality and feel during discharge.

Examples 11 to 17: Evaluation of feeling in use Compositions containing a lower alcohol, a nonionic surfactant, an acid, a cationic surfactant, and a humectant were produced according to the compositions shown in Table 3 below. The blending amounts listed in the table indicate mass %. Specifically, each component was uniformly stirred at room temperature to obtain a composition. The resulting composition was filled into a foamer container, and the quality of foam upon discharge and the feeling of use upon discharge of foam were evaluated in the following manner. The results are shown in Table 3.
<Foam quality>
・〇: Forms foam with shape retention (held for 15 seconds or more)
・△: Bubbles are formed, but the bubbles do not have sufficient shape retention (5 to 14 seconds)
・×: No bubbles / Even if bubbles are formed, they disappear immediately <Feeling when dispensing bubbles>
・〇: Not sticky when applied to the skin ・△: Slightly sticky when applied to the skin
・×: Sticky when applied to the skin

From Table 3, Examples 11 to 17, in which 40% by mass of ethanol was blended and a nonionic surfactant was blended, were able to form foam with retention, were non-sticky, and had an excellent feeling of use. From this, the composition of the present invention containing 30 to 60% by mass of lower alcohol, a nonionic surfactant, an acid, a cationic surfactant, and a humectant is a composition with excellent foam quality and feel when dispensing. I understand that there is something.

Example 18
The composition of the present invention is prepared by uniformly stirring 40% by mass of ethanol, 0.05% by mass of benzalkonium chloride, 1.0% by mass of polyoxyethylene octyl dodecyl ether, 2% by mass of glycerin, and water (the remainder) at room temperature. The resulting composition was filled into a former container. When the obtained composition was discharged from the former container, it was discharged in the form of foam, and the retention time was as long as 15 seconds or more. The foam composition does not cause dripping, can be applied uniformly to the skin or hard surfaces, and has sterilizing and disinfecting effects.

Example 19
50% by mass of ethanol, 5% by mass of isopropanol, 0.05% by mass of benzethonium chloride, 1.0% by mass of sorbitan fatty acid ester, 2% by mass of glycerin, 1% by mass of diglycerin and water (the remainder) were uniformly stirred at room temperature. A composition of the present invention was produced, and the resulting composition was filled into a former container. When the obtained composition is discharged from a foamer container, it is discharged in the form of foam, and its retention time is long. The foam composition does not cause dripping, can be applied uniformly to the skin or hard surfaces, and has sterilizing and disinfecting effects.

Example 20
The composition of the present invention was prepared by uniformly stirring 60% by mass of ethanol, 0.04% by mass of sodium hypochlorite, 1.5% by mass of polyoxyethylene behenyl ether, 2% by mass of glycerin, and water (the remainder) at room temperature. The resulting composition was filled into a former container. When the obtained composition is discharged from a foamer container, it is discharged in the form of foam, and its retention time is long. The foam composition does not cause dripping, can be applied uniformly to the skin or hard surfaces, and has sterilizing and disinfecting effects.

Example 23
60% by mass of ethanol, 0.1% by mass of lauryl dimethylamine N-oxide, 1% by mass of polyoxyethylene lauryl ether, 0.2% by mass of citric acid, 2% by mass of glycerin, and water (the remainder) were uniformly stirred at room temperature. The composition of the present invention was manufactured using the same method, and the obtained composition was filled into a former container. When the obtained composition is discharged from a foamer container, it is discharged in the form of foam, and its retention time is long. The foam composition does not cause dripping, can be applied uniformly to the skin or hard surfaces, and has sterilizing and disinfecting effects.

Although the composition of the present invention contains a lower alcohol, it has good foam retention, is comfortable to use, and has excellent bactericidal and antiviral properties, and therefore has high industrial utility.

Claims (1)

A composition that is used by discharging it in the form of a foam from a foamer container, and contains a lower alcohol selected from ethanol and isopropanol , a nonionic surfactant, an acid (excluding citric acid), and a cationic surfactant. , the nonionic surfactant is at least one selected from polyoxyethylene polyoxypropylene alkyl ether, polyoxyalkylene sorbitan fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, and glycerin fatty acid ester, and contains the lower alcohol. A composition characterized in that the amount is 30-60% by weight.
(However, the following compositions (1) to (6) are excluded.
(1) Composition containing at least one selected from lysine, serine, and sodium dl-pyrrolidonecarboxylate (2) Composition containing an alkaline agent (3) Bis-PEG/PPG-[14-20]/ [5-20] Composition containing a silicone-based surfactant, including dimethicone (4) Aerosol composition containing a propellant with a critical temperature of 0 to 50° C. (5) Monostearic acid with HLB 9 to 16 Composition containing polyoxyethylene glycerin or polyethylene glycol monostearate with HLB 9 to 16 (6) A metal ion source material selected from a copper ion source material that releases copper ions or a zinc ion source material that releases zinc ions. (composition containing)