JP7195008B2 - グリカン分析のための酵素 - Google Patents

グリカン分析のための酵素 Download PDF

Info

Publication number
JP7195008B2
JP7195008B2 JP2019565188A JP2019565188A JP7195008B2 JP 7195008 B2 JP7195008 B2 JP 7195008B2 JP 2019565188 A JP2019565188 A JP 2019565188A JP 2019565188 A JP2019565188 A JP 2019565188A JP 7195008 B2 JP7195008 B2 JP 7195008B2
Authority
JP
Japan
Prior art keywords
sialidase
seq
amino acid
composition
glycoprotein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2019565188A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020522242A5 (enExample
JP2020522242A (ja
Inventor
レオ、フレドリク
ルード、ロルフ
ビョーク、ステファン
メジャレ、マリン
オルソン、フレドリク
Original Assignee
ジェノビス エービー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1708471.6A external-priority patent/GB201708471D0/en
Priority claimed from GBGB1708476.5A external-priority patent/GB201708476D0/en
Priority claimed from GBGB1806655.5A external-priority patent/GB201806655D0/en
Application filed by ジェノビス エービー filed Critical ジェノビス エービー
Publication of JP2020522242A publication Critical patent/JP2020522242A/ja
Publication of JP2020522242A5 publication Critical patent/JP2020522242A5/ja
Application granted granted Critical
Publication of JP7195008B2 publication Critical patent/JP7195008B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24057—O-Sialoglycoprotein endopeptidase (3.4.24.57), i.e. glycoprotease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/952—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from bacteria

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
JP2019565188A 2017-05-26 2018-05-25 グリカン分析のための酵素 Active JP7195008B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GBGB1708471.6A GB201708471D0 (en) 2017-05-26 2017-05-26 Polypeptide
GB1708476.5 2017-05-26
GB1708471.6 2017-05-26
GBGB1708476.5A GB201708476D0 (en) 2017-05-26 2017-05-26 Tools for glycan analysis
GBGB1806655.5A GB201806655D0 (en) 2018-04-24 2018-04-24 Polypeptide
GB1806655.5 2018-04-24
PCT/EP2018/063833 WO2018215657A1 (en) 2017-05-26 2018-05-25 Enzymes for glycan analysis

Publications (3)

Publication Number Publication Date
JP2020522242A JP2020522242A (ja) 2020-07-30
JP2020522242A5 JP2020522242A5 (enExample) 2021-07-26
JP7195008B2 true JP7195008B2 (ja) 2022-12-23

Family

ID=62492612

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2019565188A Active JP7195008B2 (ja) 2017-05-26 2018-05-25 グリカン分析のための酵素
JP2019565178A Pending JP2020521460A (ja) 2017-05-26 2018-05-25 Ｏ−糖タンパク質のためのプロテアーゼ及び結合ポリペプチド
JP2023065981A Active JP7557896B2 (ja) 2017-05-26 2023-04-13 Ｏ－糖タンパク質のためのプロテアーゼ及び結合ポリペプチド

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2019565178A Pending JP2020521460A (ja) 2017-05-26 2018-05-25 Ｏ−糖タンパク質のためのプロテアーゼ及び結合ポリペプチド
JP2023065981A Active JP7557896B2 (ja) 2017-05-26 2023-04-13 Ｏ－糖タンパク質のためのプロテアーゼ及び結合ポリペプチド

Country Status (14)

Country Link
US (3) US11584922B2 (enExample)
EP (2) EP3630968B8 (enExample)
JP (3) JP7195008B2 (enExample)
KR (2) KR102609790B1 (enExample)
CN (2) CN111183220B (enExample)
AU (2) AU2018272400B2 (enExample)
CA (2) CA3063815A1 (enExample)
DK (2) DK3630967T3 (enExample)
ES (2) ES2986910T3 (enExample)
FI (2) FI3630967T3 (enExample)
IL (2) IL270950B2 (enExample)
PL (2) PL3630968T3 (enExample)
PT (2) PT3630968T (enExample)
WO (2) WO2018215657A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3630968B8 (en) 2017-05-26 2024-07-03 Genovis AB Enzymes for glycan analysis
JP7462560B2 (ja) 2018-01-03 2024-04-05 パレオン ファーマシューティカルズ インコーポレイテッド 組換えヒトシアリダーゼ、シアリダーゼ融合タンパク質およびそれらの使用方法
AU2020301037B2 (en) * 2019-07-03 2026-08-06 Palleon Pharmaceuticals Inc. Recombinant human sialidases, sialidase fusion proteins, and methods of using the same
CN112795583A (zh) * 2020-11-16 2021-05-14 上海大学 重组唾液酸外切酶的制备方法、表达基因、重组表达载体及构建方法
EP4448782A1 (en) * 2021-12-15 2024-10-23 Danisco US Inc. Compositions and methods involving proteases specific for mannose-modified proteins
KR20250115409A (ko) * 2022-11-28 2025-07-30 암젠 인크 에타너셉트를 프로세싱하는 방법
US20260092921A1 (en) 2024-09-27 2026-04-02 Roche Diagnostics Gmbh Mutated neuroaminidase

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6720165B2 (en) 2001-06-14 2004-04-13 Zyomix, Inc. Methods for making antibody fragments and compositions resulting therefrom
US7807174B2 (en) * 2002-11-22 2010-10-05 Nexbio, Inc. Class of therapeutic protein based molecules
GB0624874D0 (en) 2006-12-13 2007-01-24 Hansa Medical Ab Treatment
WO2009129086A2 (en) 2008-04-18 2009-10-22 New England Biolabs, Inc. Endoglycosidases that cleave o-linked glycans
EP2166085A1 (en) 2008-07-16 2010-03-24 Suomen Punainen Risti Veripalvelu Divalent modified cells
WO2010111674A2 (en) * 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
GB201115841D0 (en) 2011-09-13 2011-10-26 Genovis Ab Protein and method
GB201316744D0 (en) 2013-09-20 2013-11-06 Genovis Ab Method
WO2016003795A1 (en) 2014-07-02 2016-01-07 New England Biolabs, Inc. Universal n-glycan binding reagent
WO2017052433A1 (en) 2015-09-24 2017-03-30 Saab Ab A modular fluid actuator system
AU2017214441B2 (en) 2016-02-04 2021-08-19 Genovis Ab New Streptococcal proteases
EP3630968B8 (en) * 2017-05-26 2024-07-03 Genovis AB Enzymes for glycan analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Carbohydrate Research，2015年，Vol.415，p.60-65
Journal of Visualized Experiments，2011年，Vol.58, e3749，p.1-5

Also Published As

Publication number Publication date
EP3630968A1 (en) 2020-04-08
WO2018215657A1 (en) 2018-11-29
CN111183220B (zh) 2024-03-22
US11584922B2 (en) 2023-02-21
US12448614B2 (en) 2025-10-21
AU2018272400A1 (en) 2019-12-05
WO2018215657A9 (en) 2019-03-14
US20200087643A1 (en) 2020-03-19
WO2018215656A9 (en) 2019-03-14
JP2023103233A (ja) 2023-07-26
AU2018272400B2 (en) 2024-06-13
KR102608876B1 (ko) 2023-11-30
EP3630968B8 (en) 2024-07-03
IL270949B1 (en) 2025-02-01
US20200172889A1 (en) 2020-06-04
FI3630968T3 (fi) 2024-07-24
CN111183221B (zh) 2024-04-09
IL270950A (en) 2020-01-30
EP3630967A1 (en) 2020-04-08
ES2986557T3 (es) 2024-11-11
DK3630968T3 (da) 2024-07-29
AU2018272984B2 (en) 2024-05-23
EP3630968B1 (en) 2024-05-29
IL270950B2 (en) 2025-06-01
KR20200010322A (ko) 2020-01-30
CA3063815A1 (en) 2018-11-29
DK3630967T3 (da) 2024-08-19
KR102609790B1 (ko) 2023-12-04
EP3630967B8 (en) 2024-07-17
IL270949A (en) 2020-01-30
IL270950B1 (en) 2025-02-01
US12529044B2 (en) 2026-01-20
JP2020521460A (ja) 2020-07-27
US20230212543A1 (en) 2023-07-06
PL3630968T3 (pl) 2024-10-07
KR20200011955A (ko) 2020-02-04
WO2018215656A1 (en) 2018-11-29
ES2986910T3 (es) 2024-11-13
PL3630967T3 (pl) 2024-11-18
PT3630968T (pt) 2024-07-30
CA3063828A1 (en) 2018-11-29
FI3630967T3 (fi) 2024-08-12
IL270949B2 (en) 2025-06-01
PT3630967T (pt) 2024-08-21
JP2020522242A (ja) 2020-07-30
CN111183221A (zh) 2020-05-19
AU2018272984A1 (en) 2019-12-05
CN111183220A (zh) 2020-05-19
EP3630967B1 (en) 2024-06-12
JP7557896B2 (ja) 2024-09-30

Similar Documents

Publication Publication Date Title
US12448614B2 (en) Enzymes for glycan analysis
US11098298B2 (en) Method for recombinant production of horseshoe crab Factor C protein in protozoa
JP6076349B2 (ja) ストレプトコッカス・ピオゲネス由来のエンドグリコシダーゼ及びその使用方法
JP4248900B2 (ja) 新規なフルクトシルアミンオキシダーゼをコードする遺伝子及びそれを用いての該フルクトシルアミンオキシダーゼの製造方法
JP2025143246A (ja) ジペプチジルペプチダーゼ及びロイシンアミノペプチダーゼポリペプチドバリアント
He et al. High-level expression of human extracellular superoxide dismutase in Escherichia coli and insect cells
US20190225652A1 (en) Generation of peptides
Du et al. Secretory expression and characterization of the recombinant myofibril-bound serine proteinase of crucian carp (Carassius auratus) in Pichia pastoris
US9545431B1 (en) Isolated recombinant cDNA
US20260049292A1 (en) Use of glycogen synthase kinase-3 (gsk3) as protease, and gsk3-based proteolysis-targeting chimera (protac) and preparation method and use thereof
Ogawa et al. The pro-form of Stereum purpureum endopolygalacturonase I is inactivated by a pro-sequence in the C-terminal region
JP2016052302A (ja) キモトリプシン様プロテアーゼ

Legal Events

Date Code Title Description
RD01 Notification of change of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7426

Effective date: 20200409

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20200409

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210517

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210517

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220426

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220726

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20221108

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20221206

R150 Certificate of patent or registration of utility model

Ref document number: 7195008

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250