JP7161291B2 - 変異体ポア - Google Patents

変異体ポア Download PDF

Info

Publication number
JP7161291B2
JP7161291B2 JP2017512039A JP2017512039A JP7161291B2 JP 7161291 B2 JP7161291 B2 JP 7161291B2 JP 2017512039 A JP2017512039 A JP 2017512039A JP 2017512039 A JP2017512039 A JP 2017512039A JP 7161291 B2 JP7161291 B2 JP 7161291B2
Authority
JP
Japan
Prior art keywords
csgg
pore
polynucleotide
seq
monomers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017512039A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017527284A (ja
JP2017527284A5 (https=
Inventor
ホウォルカ，ステファン
リモート，ハン
ジャヤシンゲ，ラクマル
エリザベス，ジェーン ウォレス
アンソニー クラーク，ジェームス
ジョージ ハンブリー，リチャード
バンクス ピュー，ジョナサン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vlaams Instituut voor Biotechnologie VIB
Vrije Universiteit Brussel VUB
Original Assignee
Vlaams Instituut voor Biotechnologie VIB
Vrije Universiteit Brussel VUB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB201415455A external-priority patent/GB201415455D0/en
Priority claimed from GBGB1506754.9A external-priority patent/GB201506754D0/en
Priority claimed from GBGB1508287.8A external-priority patent/GB201508287D0/en
Priority claimed from GBGB1511203.0A external-priority patent/GB201511203D0/en
Priority claimed from GBGB1515240.8A external-priority patent/GB201515240D0/en
Application filed by Vlaams Instituut voor Biotechnologie VIB, Vrije Universiteit Brussel VUB filed Critical Vlaams Instituut voor Biotechnologie VIB
Publication of JP2017527284A publication Critical patent/JP2017527284A/ja
Publication of JP2017527284A5 publication Critical patent/JP2017527284A5/ja
Priority to JP2022133867A priority Critical patent/JP2022177005A/ja
Publication of JP7161291B2 publication Critical patent/JP7161291B2/ja
Application granted granted Critical
Priority to JP2024085524A priority patent/JP2024122996A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/116—Nucleic acid detection characterized by the use of physical, structural and functional properties electrical properties of nucleic acids, e.g. impedance, conductivity or resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2017512039A 2014-09-01 2015-09-01 変異体ポア Active JP7161291B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022133867A JP2022177005A (ja) 2014-09-01 2022-08-25 変異体ポア
JP2024085524A JP2024122996A (ja) 2014-09-01 2024-05-27 変異体ポア

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
GB1415455.3 2014-09-01
GB201415455A GB201415455D0 (en) 2014-09-01 2014-09-01 Protein pores and sensing applications
GB201422079 2014-12-11
GB1422079.2 2014-12-11
GBGB1506489.2A GB201506489D0 (en) 2014-09-01 2015-04-16 Protein pores for sensing applications
GB1506489.2 2015-04-16
GBGB1506754.9A GB201506754D0 (en) 2015-04-21 2015-04-21 Mutant pore
GB1506754.9 2015-04-21
GBGB1508287.8A GB201508287D0 (en) 2015-05-14 2015-05-14 Mutant pores
GB1508287.8 2015-05-14
GBGB1511203.0A GB201511203D0 (en) 2015-06-25 2015-06-25 Mutant pore
GB1511203.0 2015-06-25
GBGB1515240.8A GB201515240D0 (en) 2015-08-27 2015-08-27 Mutant pore
GB1515240.8 2015-08-27
PCT/EP2015/069965 WO2016034591A2 (en) 2014-09-01 2015-09-01 Mutant pores

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2020204136A Division JP2021078500A (ja) 2014-09-01 2020-12-09 変異体ポア
JP2022133867A Division JP2022177005A (ja) 2014-09-01 2022-08-25 変異体ポア

Publications (3)

Publication Number Publication Date
JP2017527284A JP2017527284A (ja) 2017-09-21
JP2017527284A5 JP2017527284A5 (https=) 2018-10-11
JP7161291B2 true JP7161291B2 (ja) 2022-10-26

Family

ID=55440449

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2017512039A Active JP7161291B2 (ja) 2014-09-01 2015-09-01 変異体ポア
JP2020204136A Pending JP2021078500A (ja) 2014-09-01 2020-12-09 変異体ポア
JP2022133867A Pending JP2022177005A (ja) 2014-09-01 2022-08-25 変異体ポア
JP2024085524A Pending JP2024122996A (ja) 2014-09-01 2024-05-27 変異体ポア

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2020204136A Pending JP2021078500A (ja) 2014-09-01 2020-12-09 変異体ポア
JP2022133867A Pending JP2022177005A (ja) 2014-09-01 2022-08-25 変異体ポア
JP2024085524A Pending JP2024122996A (ja) 2014-09-01 2024-05-27 変異体ポア

Country Status (8)

Country Link
US (4) US10400014B2 (https=)
EP (2) EP4053150A1 (https=)
JP (4) JP7161291B2 (https=)
KR (3) KR102851011B1 (https=)
CN (7) CN114605507B (https=)
AU (1) AU2015310913B2 (https=)
CA (1) CA2959220A1 (https=)
WO (1) WO2016034591A2 (https=)

Families Citing this family (140)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP4737389A2 (en) 2011-05-27 2026-05-06 Oxford Nanopore Technologies PLC Coupling method
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
CA2879261C (en) 2012-07-19 2022-12-06 Oxford Nanopore Technologies Limited Modified helicases
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN111534504B (zh) 2014-01-22 2024-06-21 牛津纳米孔科技公开有限公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
CA2959220A1 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant csgg pores
EP3204511B1 (en) 2014-10-07 2021-07-28 Oxford Nanopore Technologies Limited Mutant pores
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
GB201508669D0 (en) 2015-05-20 2015-07-01 Oxford Nanopore Tech Ltd Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown
US10641764B2 (en) 2015-07-02 2020-05-05 The University Of Massachusetts Membrane and droplet-interface bilayer systems and methods
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
EP4397970A3 (en) 2016-04-06 2024-10-09 Oxford Nanopore Technologies PLC Mutant pore
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN109196116B (zh) 2016-05-25 2022-09-27 牛津纳米孔科技公开有限公司 一种表征靶多核苷酸的方法
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201617886D0 (en) 2016-10-21 2016-12-07 Oxford Nanopore Technologies Limited Method
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
CA3053086A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
US11119054B2 (en) * 2017-03-16 2021-09-14 The Penn State Research Foundation Versatile display scaffolds for proteins
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
GB201707138D0 (en) * 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
AU2018290975B2 (en) 2017-06-29 2024-10-03 President And Fellows Of Harvard College Deterministic stepping of polymers through a nanopore
CN117106038B (zh) * 2017-06-30 2025-12-09 弗拉芒区生物技术研究所 新颖蛋白孔
JP7012841B2 (ja) * 2017-10-23 2022-01-28 エフ．ホフマン－ラ ロシュ アーゲー 浸透性の不均衡を利用した膜内のタンパク質ナノポアの除去および再挿入
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
KR102796115B1 (ko) 2018-05-24 2025-04-14 옥스포드 나노포어 테크놀로지즈 피엘씨 전기 습윤 소자 내 액적 계면
GB201808556D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
JP7412001B2 (ja) * 2018-06-08 2024-01-12 株式会社モダリス 改変されたＣａｓ９タンパク質及びその用途
GB201812615D0 (en) 2018-08-02 2018-09-19 Ucl Business Plc Membrane bound nucleic acid nanopores
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
CN112823392B (zh) * 2018-10-12 2024-09-03 生命科技股份有限公司 用于评估微卫星不稳定性状态的方法和系统
CA3118808A1 (en) 2018-11-08 2020-05-14 Oxford Nanopore Technologies Limited Pore
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
GB201819378D0 (en) 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
CN113164954B (zh) 2018-11-28 2023-10-20 牛津纳米孔科技公开有限公司 感测系统和操作方法
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
CN111499705B (zh) * 2019-01-30 2023-08-08 深圳市梅丽纳米孔科技有限公司 一种突变NfpAB纳米孔、测试系统及制作方法与应用
JP7492200B2 (ja) 2019-03-12 2024-05-29 オックスフォード ナノポール テクノロジーズ ピーエルシー ナノ細孔センシングデバイスと操作方法およびその成形方法
GB2580988B (en) 2019-03-19 2022-04-13 Oxford Nanopore Tech Ltd Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
JP2022529623A (ja) * 2019-04-09 2022-06-23 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
US11926819B2 (en) 2019-05-28 2024-03-12 The Regents Of The University Of California Methods of adding polymers to ribonucleic acids
GB201915480D0 (en) 2019-10-25 2019-12-11 Oxford Nanopore Tech Ltd Improved nanopore sensing device, components and method of manufacture
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
CN114761799A (zh) 2019-12-02 2022-07-15 牛津纳米孔科技公开有限公司 使用纳米孔表征靶多肽的方法
GB201917742D0 (en) 2019-12-04 2020-01-15 Oxford Nanopore Tech Ltd Method
EP4127234A4 (en) * 2020-03-25 2024-05-01 Universal Sequencing Technology Corporation Enzyme mutants directly attached to a nanogap device
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
GB202016874D0 (en) 2020-10-23 2020-12-09 Oxford Nanopore Tech Ltd Nanopore support structure and manufacture thereof
GB202009349D0 (en) 2020-06-18 2020-08-05 Oxford Nanopore Tech Ltd Method
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
JP2023530155A (ja) 2020-06-18 2023-07-13 オックスフォード ナノポール テクノロジーズ ピーエルシー ナノポアを通って移動するポリヌクレオチドを特徴付ける方法
EP4675256A3 (en) 2020-07-17 2026-01-28 Oxford Nanopore Technologies PLC Nanopore sensing device
US12535457B2 (en) 2020-07-22 2026-01-27 Oxford Nanopore Technologies Plc Solid state nanopore formation
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
EP4341433A1 (en) 2021-05-19 2024-03-27 Oxford Nanopore Technologies PLC Methods for complement strand sequencing
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
CN113651876B (zh) * 2021-08-18 2024-02-02 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
US20250136647A1 (en) * 2021-08-18 2025-05-01 Qitan Technology Ltd., Chengdu Mutant of porin monomer, protein pore and application thereof
CN113480620B (zh) * 2021-08-18 2024-02-06 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
WO2023019470A1 (zh) * 2021-08-18 2023-02-23 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
GB2610380A (en) 2021-08-23 2023-03-08 Cambridge Entpr Ltd Nucleic acid detection
GB202112235D0 (en) 2021-08-26 2021-10-13 Oxford Nanopore Tech Ltd Nanopore
CN113735948B (zh) * 2021-09-28 2024-02-02 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
GB202113935D0 (en) 2021-09-29 2021-11-10 Cambridge Entpr Ltd Nucleic acid characterisation
CN113773373B (zh) * 2021-10-12 2024-02-06 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
CN113896776B (zh) * 2021-10-12 2024-02-06 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
CN113754743B (zh) * 2021-10-12 2024-02-02 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
CN113912683B (zh) * 2021-10-12 2024-02-06 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
WO2023094806A1 (en) 2021-11-29 2023-06-01 Oxford Nanopore Technologies Plc Nanopore measurement signal analysis
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
CN114957412B (zh) * 2022-04-28 2024-09-17 清华大学 一种新型孔蛋白单体及其应用
JP2025517736A (ja) 2022-05-17 2025-06-10 オックスフォード ナノポール テクノロジーズ ピーエルシー 方法及びアダプター
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
KR20250048551A (ko) 2022-08-09 2025-04-09 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어
KR20250044691A (ko) 2022-08-09 2025-04-01 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어 단량체 및 포어
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
KR20250116014A (ko) 2022-10-28 2025-07-31 리엑스유니버시테이트 그로닝겐 나노기공-기반 단백질 분석
AU2023366350A1 (en) 2022-10-28 2025-03-27 Oxford Nanopore Technologies Plc Pore monomers and pores
GB202216162D0 (en) 2022-10-31 2022-12-14 Oxford Nanopore Tech Plc Method
CN120202306A (zh) 2022-11-01 2025-06-24 牛津纳米孔科技公开有限公司 生化分析系统以及控制生化分析系统的方法
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024138470A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 纳米孔传感器及其在测序中的应用
CN120476132A (zh) * 2022-12-29 2025-08-12 深圳华大生命科学研究院 新型孔蛋白bcp34、其突变体及其应用
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202317028D0 (en) 2023-11-06 2023-12-20 Oxford Nanopore Tech Ltd Method
WO2025120150A1 (en) 2023-12-08 2025-06-12 Oxford Nanopore Technologies Plc Methods and apparatus for forming apertures, methods and apparatus for unblocking apertures, methods of sensing molecular entities in apertures, and measurement systems
GB202401864D0 (en) 2024-02-12 2024-03-27 Fund Centre De Regulacio Genòmica A method of detecting non-canonical bases in sequencing data
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
WO2025230413A1 (en) * 2024-04-30 2025-11-06 Rijksuniversiteit Groningen Ion-selective nanopore systems and methods for single-molecule polymer profiling.
WO2025236248A1 (zh) * 2024-05-16 2025-11-20 北京普译生物科技有限公司 HfaB新型纳米孔蛋白突变体及其在测序中的应用
GB202407044D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Polymer analysis using transformer neural network
GB202407099D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407100D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
GB202407228D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
WO2026052811A2 (en) 2024-09-06 2026-03-12 Oxford Nanopore Technologies Plc Method
WO2026052817A1 (en) 2024-09-06 2026-03-12 Oxford Nanopore Technologies Plc Method
GB202413506D0 (en) 2024-09-13 2024-10-30 Oxford Nanopore Tech Plc Pore monomers and their uses
WO2026057755A1 (en) 2024-09-13 2026-03-19 Oxford Nanopore Technologies Plc Adaptors and kits for rna molecules labelling and characterising
GB202413618D0 (en) 2024-09-16 2024-10-30 Oxford Nanopore Tech Plc peptide characterisation
GB2644084A (en) 2024-09-16 2026-03-18 Oxford Nanopore Tech Plc Augmented consensus and variant calling
GB202413747D0 (en) 2024-09-18 2024-10-30 Oxford Nanopore Tech Plc Preprocessing nanopore signals
GB202414276D0 (en) 2024-09-27 2024-11-13 Oxford Nanopore Tech Plc Method

Family Cites Families (167)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE56026B1 (en) 1982-10-19 1991-03-27 Cetus Corp Cysteine-depleted muteins of biologically active proteins
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
WO1994025616A1 (en) 1993-04-28 1994-11-10 Worcester Foundation For Experimental Biology Cell-targeted lytic pore-forming agents
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
DE4320201A1 (de) 1993-06-18 1995-01-12 Asta Medica Ag Verwendung von Cetrorelix und weiteren Nona- und Dekapeptiden zur Herstellung eines Arzneimittels zur Bekämpfung von Aids und zur Wachstumsstimulation
US5386373A (en) 1993-08-05 1995-01-31 Pavilion Technologies, Inc. Virtual continuous emission monitoring system with sensor validation
US7569341B2 (en) 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US5561043A (en) 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
JP3891620B2 (ja) 1996-11-14 2007-03-14 株式会社アイシン・コスモス研究所 ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法
US20020197614A1 (en) 1997-05-16 2002-12-26 Mosaic Technologies, Inc. Electrophoretic analysis of target molecules using adapter molecules
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型ｒｎａ−ｄｎａキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US7155344B1 (en) 1998-07-27 2006-12-26 Caliper Life Sciences, Inc. Distributed database for analytical instruments
US6150112A (en) 1998-09-18 2000-11-21 Yale University Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP2383776B1 (en) 1999-06-22 2015-02-25 President and Fellows of Harvard College Solid state nanopore device for evaluating biopolymers
US7087729B1 (en) 1999-06-29 2006-08-08 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
EP1208207A2 (de) 1999-08-31 2002-05-29 Michael Niederweis Verfahren zur herstellung eines kanalbildenden proteins
WO2001040516A2 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
WO2001059453A2 (en) 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
WO2001062943A1 (en) 2000-02-25 2001-08-30 Invitrogen Corporation Topoisomerase linker-mediated amplification methods
US20020132978A1 (en) 2000-03-21 2002-09-19 Hans-Peter Gerber VEGF-modulated genes and methods employing them
CA2404216A1 (en) 2000-03-22 2001-09-27 Curagen Corporation Novel polypeptides, and nucleic acids encoding the same
US20030040505A1 (en) * 2000-03-31 2003-02-27 The Regents Of The University Of California Synthetic phospholipids to ameliorate atherosclerosis and other inflammatory conditions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU2001289914A1 (en) 2000-09-25 2002-04-02 Sensovation Ag Image sensor device, apparatus and method for optical measurements
WO2002042496A2 (en) 2000-11-27 2002-05-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US20030087232A1 (en) 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
US6995236B2 (en) 2001-05-08 2006-02-07 Riken Sphingomyelin detecting probe
JP2002355035A (ja) 2001-05-08 2002-12-10 Inst Of Physical & Chemical Res スフィンゴミエリン検出プローブ
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
WO2003004992A2 (en) 2001-07-03 2003-01-16 The Regents Of The University Of California Mammalian sweet and amino acid heterodimeric taste receptors
DE60223050T2 (de) 2001-08-31 2008-07-17 Gentex Corp., Zeeland Fahrzeuglampenanordnung mit kühlkörper
AU2003214031A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S An improved method for synthesising templated molecules
EP1504114B1 (en) 2002-05-10 2017-07-12 The Texas A & M University System Stochastic sensing through covalent interactions
US20040209299A1 (en) 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
AU2004225520A1 (en) 2003-03-25 2004-10-14 Stratagene DNA polymerase fusions and uses thereof
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
US7344882B2 (en) 2003-05-12 2008-03-18 Bristol-Myers Squibb Company Polynucleotides encoding variants of the TRP channel family member, LTRPC3
US20070122885A1 (en) 2005-08-22 2007-05-31 Fermalogic, Inc. Methods of increasing production of secondary metabolites by manipulating metabolic pathways that include methylmalonyl-coa
AU2003904237A0 (en) 2003-08-08 2003-08-21 Garvan Institute Of Medical Research Novel translocation assay
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
US7424370B2 (en) 2004-02-06 2008-09-09 Council Of Scientific And Industrial Research Computational method for identifying adhesin and adhesin-like proteins of therapeutic potential
JP2005253427A (ja) 2004-03-15 2005-09-22 Aisin Seiki Co Ltd 核酸検出方法及び核酸単離方法
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US7972858B2 (en) 2004-08-13 2011-07-05 President And Fellows Of Harvard College Ultra high-throughput opti-nanopore DNA readout platform
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
CA2489194A1 (en) * 2004-12-03 2006-06-03 Guilhem Janbon Polypeptides involved in candida biofilm formation and uses thereof
CA2526272A1 (en) * 2004-12-03 2006-06-03 Institut Pasteur Polypeptides involved in candida biofilm formation and uses thereof
EP1842061A4 (en) 2004-12-21 2009-05-13 Texas A & M Univ Sys HIGH-TEMPERATURE ION CHANNELS AND ION PORES
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7272518B2 (en) 2005-07-01 2007-09-18 Square D Company Automated hierarchy classification in utility monitoring systems
US7877154B2 (en) 2005-09-30 2011-01-25 Fisher-Rosemount Systems, Inc. Method and system for controlling a batch process
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
WO2007075987A2 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
WO2007119880A1 (en) * 2006-04-13 2007-10-25 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family which has been modified to abolish curli formation
US7849581B2 (en) 2006-05-05 2010-12-14 University Of Utah Research Foundation Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof
CA2663047A1 (en) 2006-09-06 2008-03-13 Phase Bioscience, Inc. Therapeutic elastin-like polypeptide (elp) fusion proteins
US7638034B2 (en) 2006-09-21 2009-12-29 Los Alamos National Security, Llc Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions
US20100311602A1 (en) 2006-10-13 2010-12-09 J. Craig Venter Institute, Inc. Sequencing method
US8594848B2 (en) 2006-11-28 2013-11-26 Lester F. Ludwig Reconfigurable chemical process systems
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
EP2156179B1 (en) 2007-04-04 2021-08-18 The Regents of The University of California Methods for using a nanopore
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
US8158344B2 (en) 2007-06-29 2012-04-17 Duke University Methods and compositions for correlating genetic markers with multiple sclerosis
GB0716005D0 (en) 2007-08-16 2007-09-26 Imp Innovations Ltd Single molecule spectroscopy using nanoporpus membranes
GB0716264D0 (en) 2007-08-21 2007-09-26 Isis Innovation Bilayers
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
WO2009084721A1 (en) 2007-12-31 2009-07-09 Fujirebio Inc. Clusters of microresonators for cavity mode optical sensing
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
EP4230747A3 (en) 2008-03-28 2023-11-15 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
EP2293921A4 (en) 2008-05-22 2013-05-22 Univ California MEMBRANE PROBLEMS AND MEMBRANES MADE FROM THESE
US8652771B2 (en) 2008-05-28 2014-02-18 University of Souther California Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects
EP2310534B1 (en) 2008-07-07 2018-09-05 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) * 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US8481264B2 (en) * 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
CA2774710C (en) 2008-09-22 2016-08-02 University Of Washington Msp nanopores and related methods
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US20110246084A1 (en) 2008-11-26 2011-10-06 Mostafa Ronaghi Methods and systems for analysis of sequencing data
KR20110125226A (ko) 2009-01-30 2011-11-18 옥스포드 나노포어 테크놀로지즈 리미티드 혼성화 링커
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
AU2010215761B2 (en) 2009-02-23 2017-04-06 Cytomx Therapeutics, Inc Proproteins and methods of use thereof
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
FR2943656A1 (fr) 2009-03-25 2010-10-01 Air Liquide Procede et installation de production d'hydrogene mettant en oeuvre un compresseur thermocinetique
EP2414515A2 (en) * 2009-04-01 2012-02-08 Danisco US Inc. Cleaning system comprising an alpha-amylase and a protease
CN102405410B (zh) 2009-04-20 2014-06-25 牛津楠路珀尔科技有限公司 脂质双层传感器阵列
US8402071B2 (en) 2009-06-19 2013-03-19 Aptare, Inc. Catalog that stores file system metadata in an optimized manner
US9127313B2 (en) 2009-12-01 2015-09-08 Oxford Nanopore Technologies Limited Biochemical analysis instrument
FR2955773B1 (fr) 2010-02-01 2017-05-26 Commissariat Energie Atomique Complexe moleculaire de ciblage des antigenes vers les cellules presentatrices d'antigene et ses applications pour la vaccination
CN102834716B (zh) 2010-02-23 2016-03-30 华盛顿大学 人工分枝菌酸膜
WO2011125015A2 (en) 2010-04-05 2011-10-13 Bar-Ilan University Protease-activatable pore-forming polypeptides
EA037276B1 (ru) * 2010-04-27 2021-03-03 Лисандо Аг Способы устранения или уменьшения бактериальной биопленки
US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
WO2012042226A2 (en) 2010-10-01 2012-04-05 Oxford Nanopore Technologies Limited Biochemical analysis apparatus and rotary valve
CN102116783B (zh) 2010-12-31 2013-05-29 北京普源精电科技有限公司 一种波形显示方法
GB201100516D0 (en) 2011-01-12 2011-02-23 Isis Innovation Method using fluorinated amphiphiles
CN102174554A (zh) 2011-01-24 2011-09-07 内蒙古民族大学 双控双调节原核表达载体系统及其构建方法和用途
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
EP4737389A2 (en) 2011-05-27 2026-05-06 Oxford Nanopore Technologies PLC Coupling method
WO2012166906A1 (en) 2011-05-31 2012-12-06 Massachusetts Institute Of Technology Cell-directed synthesis of multifunctional nanopatterns and nanomaterials
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
KR101409266B1 (ko) * 2011-11-24 2014-06-24 영남대학교 산학협력단 바이오필름 억제제 및 억제방법
AU2012360244B2 (en) 2011-12-29 2018-08-23 Oxford Nanopore Technologies Limited Enzyme method
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
CN107828877A (zh) 2012-01-20 2018-03-23 吉尼亚科技公司 基于纳米孔的分子检测与测序
IN2014DN06796A (https=) 2012-02-15 2015-05-22 Oxford Nanopore Tech Ltd
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
TWI655213B (zh) 2012-07-13 2019-04-01 Menicon Co., Ltd. 自我組織化肽衍生物的製造方法
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
CA2879261C (en) 2012-07-19 2022-12-06 Oxford Nanopore Technologies Limited Modified helicases
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
US20150291991A1 (en) * 2012-11-16 2015-10-15 President And Fellows Of Harvard College Methods and compositions relating to amyloidogenic polypeptides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US20140206842A1 (en) 2013-01-22 2014-07-24 Muhammed Majeed Peptides Modified with Triterpenoids and Small Organic Molecules: Synthesis and use in Cosmeceutical
US10179933B2 (en) 2013-02-07 2019-01-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Hybrid nanopores and uses thereof for detection of analytes
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
DK2970951T3 (da) 2013-03-13 2019-05-13 Illumina Inc Fremgangsmåder til nukleinsyresekventering
US10613076B2 (en) 2013-03-14 2020-04-07 The Trustees Of Boston University Optoelectronic control of solid-state nanopores
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
AU2014270410B2 (en) 2013-05-24 2020-07-16 Illumina Cambridge Limited Pyrophosphorolytic sequencing
WO2015051378A1 (en) 2013-10-04 2015-04-09 University Of Washington Through Its Center For Commercialization Systems and methods for nanopore-based analysis of nucleic acids
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
SI3074534T1 (sl) 2013-11-26 2019-08-30 Illumina, Inc. Postopki določanja zaporedja polinukleotidov
WO2015097289A1 (en) 2013-12-24 2015-07-02 Vib Vzw Secretion and functional display of chimeric polypeptides
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN111534504B (zh) 2014-01-22 2024-06-21 牛津纳米孔科技公开有限公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
US9925679B2 (en) 2014-05-19 2018-03-27 I+D+M Creative, Llc Devices and methods for assisting with slicing items
FR3023394B1 (fr) 2014-07-02 2017-12-29 Adn Access Data Networks Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture
CA2959220A1 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant csgg pores
EP3204511B1 (en) 2014-10-07 2021-07-28 Oxford Nanopore Technologies Limited Mutant pores
US10480026B2 (en) 2014-10-17 2019-11-19 Oxford Nanopore Technologies Ltd. Method for nanopore RNA characterisation
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
EP4397970A3 (en) 2016-04-06 2024-10-09 Oxford Nanopore Technologies PLC Mutant pore
CA3053086A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106038B (zh) 2017-06-30 2025-12-09 弗拉芒区生物技术研究所 新颖蛋白孔
CA3118808A1 (en) 2018-11-08 2020-05-14 Oxford Nanopore Technologies Limited Pore

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Ｅｐｓｔｅｉｎ， Ｅｌｉｓａｂｅｔｈ Ａｓｈｍａｎ， "Ａｓｓｅｍｂｌｙ， Ｓｐａｔｉａｌ Ｄｉｓｔｒｉｂｕｔｉｏｎ， ａｎｄ Ｓｅｃｒｅｔｉｏｎ Ａｃｔｉｖｉｔｙ ｏｆ ｔｈｅ Ｃｕｒｌｉｎ Ｓｅｃｒｅｔｉｏｎ Ｌｉｐｏｐｒｏｔｅｉｎ， ＣｓｇＧ．"， Ｄｅｅｐ Ｂｌｕｅ Ｒｅｐｏｓｉｔｏｒｉｅｓ［ｏｎｌｉｎｅ］， ２００８年， Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ ｏｆ Ｍｉｃｈｉｇａｎ， Ａｎｎ Ａｒｂｏｒ， ＭＩ， ＵＳＡ．［ｒｅｔｒｉｅｖｅｄ ｏｎ １ Ｊｕｎｅ ２０２２］， Ｒｅｔｒｉｅｖｅｄ ｆｒｏｍ ｔｈｅ Ｉｎｔｅｒｎｅｔ：＜ＵＲＬ：ｈｔｔｐｓ：／／ｄｅｅｐｂｌｕｅ．ｌｉｂ．ｕｍｉｃｈ．ｅｄｕ／ｂｉｔｓｔｒｅａｍ／ｈａｎｄｌｅ／２０２７．４２／６１６６７／ｅｍａｓｈｍａｎ＿１．ｐｄｆ＞， ＩＳＢＮ： ９７８０５４９９８４８５６
ＰＮＡＳ， ２０１４年１２月１日， Ｖｏｌ．１１１， Ｎｏ．５０， ｐｐ．Ｅ５４３９－Ｅ５４４４

Also Published As

Publication number Publication date
EP3224274B1 (en) 2024-02-14
KR102851011B1 (ko) 2025-08-27
US11034734B2 (en) 2021-06-15
US20210292376A1 (en) 2021-09-23
CN117164683A (zh) 2023-12-05
EP4053150A1 (en) 2022-09-07
US12275761B2 (en) 2025-04-15
JP2024122996A (ja) 2024-09-10
JP2017527284A (ja) 2017-09-21
KR20230125853A (ko) 2023-08-29
JP2021078500A (ja) 2021-05-27
AU2015310913B2 (en) 2021-04-01
KR20250130441A (ko) 2025-09-01
CN117700500B (zh) 2026-02-24
CA2959220A1 (en) 2016-03-10
CN107207570A (zh) 2017-09-26
CN117700500A (zh) 2024-03-15
CN119899249A (zh) 2025-04-29
CN117164682A (zh) 2023-12-05
AU2015310913A1 (en) 2017-03-16
WO2016034591A3 (en) 2016-06-09
CN114605507A (zh) 2022-06-10
KR20170042794A (ko) 2017-04-19
US20260015392A1 (en) 2026-01-15
EP3224274A2 (en) 2017-10-04
JP2022177005A (ja) 2022-11-30
US20170283470A1 (en) 2017-10-05
US10400014B2 (en) 2019-09-03
WO2016034591A2 (en) 2016-03-10
US20200017556A1 (en) 2020-01-16
US20180148481A2 (en) 2018-05-31
CN117164684A (zh) 2023-12-05
CN114605507B (zh) 2026-01-06

Similar Documents

Publication Publication Date Title
JP7161291B2 (ja) 変異体ポア
US12227800B2 (en) Mutant pores
JP2017503517A (ja) １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
HK40080435A (en) Mutant csgg pores
HK40067060A (zh) 突变csgg孔

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180831

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180831

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191029

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200129

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200327

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20200811

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201209

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20201209

C11 Written invitation by the commissioner to file amendments

Free format text: JAPANESE INTERMEDIATE CODE: C11

Effective date: 20201222

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20210416

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20210420

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20210618

C211 Notice of termination of reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C211

Effective date: 20210622

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20210831

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20211012

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20220118

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20220426

C27C Written answer of applicant after an oral proceeding

Free format text: JAPANESE INTERMEDIATE CODE: C2721

Effective date: 20220426

C876 Explanation why request for accelerated appeal examination is justified

Free format text: JAPANESE INTERMEDIATE CODE: C876

Effective date: 20220511

C305 Report on accelerated appeal examination

Free format text: JAPANESE INTERMEDIATE CODE: C305

Effective date: 20220517

C13 Notice of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: C13

Effective date: 20220614

C28A Non-patent document cited

Free format text: JAPANESE INTERMEDIATE CODE: C2838

Effective date: 20220614

C302 Record of communication

Free format text: JAPANESE INTERMEDIATE CODE: C302

Effective date: 20220823

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220825

C23 Notice of termination of proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C23

Effective date: 20220913

C03 Trial/appeal decision taken

Free format text: JAPANESE INTERMEDIATE CODE: C03

Effective date: 20221011

C30A Notification sent

Free format text: JAPANESE INTERMEDIATE CODE: C3012

Effective date: 20221011

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20221014

R150 Certificate of patent or registration of utility model

Ref document number: 7161291

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250