JP7100584B2 - グリコシル化されたタンパク質を産生する方法 - Google Patents

グリコシル化されたタンパク質を産生する方法 Download PDF

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JP7100584B2
JP7100584B2 JP2018546761A JP2018546761A JP7100584B2 JP 7100584 B2 JP7100584 B2 JP 7100584B2 JP 2018546761 A JP2018546761 A JP 2018546761A JP 2018546761 A JP2018546761 A JP 2018546761A JP 7100584 B2 JP7100584 B2 JP 7100584B2
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protein
host cell
target protein
optionally
glucose
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JP2019502401A (ja
JP2019502401A5 (https=
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エル． ウェッター マイケル
ティー． コワリク マイケル
ファリドモアイエル アミルレザ
マリー マヌエラ
エー． ライザック クリスチャン
アエビ マルクス
リン チア‐ウェイ
ハング イバン
キーズ ティモシー
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リンマテック バイオロジクス エージー
エイドゲノッシスチェ テクニスチェ ホチスチュレ チューリッヒ
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07043—N-Acylneuraminate cytidylyltransferase (2.7.7.43)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Molecular Biology (AREA)
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  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2018546761A 2015-11-30 2016-11-30 グリコシル化されたタンパク質を産生する方法 Expired - Fee Related JP7100584B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562260725P 2015-11-30 2015-11-30
US62/260,725 2015-11-30
US201662416853P 2016-11-03 2016-11-03
US62/416,853 2016-11-03
PCT/EP2016/079232 WO2017093291A1 (en) 2015-11-30 2016-11-30 Methods of producing glycosylated proteins

Publications (3)

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JP2019502401A JP2019502401A (ja) 2019-01-31
JP2019502401A5 JP2019502401A5 (https=) 2020-01-30
JP7100584B2 true JP7100584B2 (ja) 2022-07-13

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US (2) US11236136B2 (https=)
EP (1) EP3384018A1 (https=)
JP (1) JP7100584B2 (https=)
CN (1) CN108779443A (https=)
AU (1) AU2016363711A1 (https=)
CA (1) CA3006400A1 (https=)
WO (1) WO2017093291A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019002512A2 (en) 2017-06-30 2019-01-03 Limmatech Biologics Ag GLYCOPROTEINS CUSTOMIZED FROM ENGINEERING AND FULLY FUNCTIONAL
WO2019035916A1 (en) * 2017-08-15 2019-02-21 Northwestern University DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES
WO2020044371A1 (en) * 2018-08-29 2020-03-05 Council Of Scientific And Industrial Research Recombinant microbial system for directed evolution of glycocins and a method of preparation thereof
CN109486689B (zh) * 2018-12-27 2020-06-16 江南大学 一种增强l-天冬酰胺酶耐酸性的方法
US20220186276A1 (en) * 2019-01-25 2022-06-16 Northwestern University Platform for producing glycoproteins, identifying glycosylation pathways
CN110117631A (zh) * 2019-05-24 2019-08-13 山东大学 一种利用三种糖基转移酶制备Globotriose-连接的活性糖肽的方法
KR102371465B1 (ko) * 2019-11-08 2022-03-08 영남대학교 산학협력단 코돈 최적화된 캄필로박터제주니 유래 ｎ-아세틸갈락토사민 전이효소와 갈락토즈 전이효소, 및 이를 이용한 대장균 기반의 가용성 전이효소 생산 방법
WO2022053673A1 (en) 2020-09-14 2022-03-17 Limmatech Biologics Ag Fab sialylation of antibodies

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014145180A1 (en) 2013-03-15 2014-09-18 Glycobia, Inc. Polysialic acid, blood group antigens and glycoprotein expression
JP2014529396A (ja) 2011-08-08 2014-11-13 イーティーエイチ・チューリッヒ パスツレラワクチン

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1730293A2 (en) 2004-03-17 2006-12-13 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
PT1991690E (pt) 2006-03-09 2014-04-22 Centre Nat Rech Scient Método para a produção de oligossacáridos sialilados
EP2501406B8 (en) 2009-11-19 2018-01-24 GlaxoSmithKline Biologicals S.A. Biosynthetic system that produces immunogenic polysaccharides in prokaryotic cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014529396A (ja) 2011-08-08 2014-11-13 イーティーエイチ・チューリッヒ パスツレラワクチン
WO2014145180A1 (en) 2013-03-15 2014-09-18 Glycobia, Inc. Polysialic acid, blood group antigens and glycoprotein expression

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Publication number Publication date
AU2016363711A9 (en) 2018-12-13
WO2017093291A9 (en) 2018-07-12
US20180354997A1 (en) 2018-12-13
US20220242919A1 (en) 2022-08-04
AU2016363711A1 (en) 2018-06-14
CN108779443A (zh) 2018-11-09
EP3384018A1 (en) 2018-10-10
US11236136B2 (en) 2022-02-01
WO2017093291A1 (en) 2017-06-08
CA3006400A1 (en) 2017-06-08
JP2019502401A (ja) 2019-01-31

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