CN108779443A - 产生糖基化蛋白的方法 - Google Patents

产生糖基化蛋白的方法 Download PDF

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Publication number
CN108779443A
CN108779443A CN201680080072.9A CN201680080072A CN108779443A CN 108779443 A CN108779443 A CN 108779443A CN 201680080072 A CN201680080072 A CN 201680080072A CN 108779443 A CN108779443 A CN 108779443A
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CN
China
Prior art keywords
host cell
protein
glycosylation
ngt
target protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680080072.9A
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English (en)
Chinese (zh)
Inventor
迈克尔·L·韦特尔
迈克尔·T·科瓦里克
阿米尔礼萨·法里德莫亚耶
曼纽拉·玛莉
克里斯蒂安·A·利扎克
M·阿比
林嘉伟
伊凡·汉格
蒂莫西·基斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Limtec Biological Products Co
Eidgenoessische Technische Hochschule Zurich ETHZ
Original Assignee
Limtec Biological Products Co
Eidgenoessische Technische Hochschule Zurich ETHZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Limtec Biological Products Co, Eidgenoessische Technische Hochschule Zurich ETHZ filed Critical Limtec Biological Products Co
Publication of CN108779443A publication Critical patent/CN108779443A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07043—N-Acylneuraminate cytidylyltransferase (2.7.7.43)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201680080072.9A 2015-11-30 2016-11-30 产生糖基化蛋白的方法 Pending CN108779443A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562260725P 2015-11-30 2015-11-30
US62/260,725 2015-11-30
US201662416853P 2016-11-03 2016-11-03
US62/416,853 2016-11-03
PCT/EP2016/079232 WO2017093291A1 (en) 2015-11-30 2016-11-30 Methods of producing glycosylated proteins

Publications (1)

Publication Number Publication Date
CN108779443A true CN108779443A (zh) 2018-11-09

Family

ID=57460500

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680080072.9A Pending CN108779443A (zh) 2015-11-30 2016-11-30 产生糖基化蛋白的方法

Country Status (7)

Country Link
US (2) US11236136B2 (https=)
EP (1) EP3384018A1 (https=)
JP (1) JP7100584B2 (https=)
CN (1) CN108779443A (https=)
AU (1) AU2016363711A1 (https=)
CA (1) CA3006400A1 (https=)
WO (1) WO2017093291A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109486689A (zh) * 2018-12-27 2019-03-19 江南大学 一种增强l-天冬酰胺酶耐酸性的方法
CN110117631A (zh) * 2019-05-24 2019-08-13 山东大学 一种利用三种糖基转移酶制备Globotriose-连接的活性糖肽的方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019002512A2 (en) 2017-06-30 2019-01-03 Limmatech Biologics Ag GLYCOPROTEINS CUSTOMIZED FROM ENGINEERING AND FULLY FUNCTIONAL
WO2019035916A1 (en) * 2017-08-15 2019-02-21 Northwestern University DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES
WO2020044371A1 (en) * 2018-08-29 2020-03-05 Council Of Scientific And Industrial Research Recombinant microbial system for directed evolution of glycocins and a method of preparation thereof
US20220186276A1 (en) * 2019-01-25 2022-06-16 Northwestern University Platform for producing glycoproteins, identifying glycosylation pathways
KR102371465B1 (ko) * 2019-11-08 2022-03-08 영남대학교 산학협력단 코돈 최적화된 캄필로박터제주니 유래 ｎ-아세틸갈락토사민 전이효소와 갈락토즈 전이효소, 및 이를 이용한 대장균 기반의 가용성 전이효소 생산 방법
WO2022053673A1 (en) 2020-09-14 2022-03-17 Limmatech Biologics Ag Fab sialylation of antibodies

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005090552A2 (en) * 2004-03-17 2005-09-29 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
WO2013020988A1 (en) * 2011-08-08 2013-02-14 Eth Zurich Pasteurellaceae vaccines
WO2014145180A1 (en) * 2013-03-15 2014-09-18 Glycobia, Inc. Polysialic acid, blood group antigens and glycoprotein expression

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1991690E (pt) 2006-03-09 2014-04-22 Centre Nat Rech Scient Método para a produção de oligossacáridos sialilados
EP2501406B8 (en) 2009-11-19 2018-01-24 GlaxoSmithKline Biologicals S.A. Biosynthetic system that produces immunogenic polysaccharides in prokaryotic cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005090552A2 (en) * 2004-03-17 2005-09-29 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
WO2013020988A1 (en) * 2011-08-08 2013-02-14 Eth Zurich Pasteurellaceae vaccines
WO2014145180A1 (en) * 2013-03-15 2014-09-18 Glycobia, Inc. Polysialic acid, blood group antigens and glycoprotein expression

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
THERESA LINDHOUT ET AL.: "Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes", 《PNAS》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109486689A (zh) * 2018-12-27 2019-03-19 江南大学 一种增强l-天冬酰胺酶耐酸性的方法
CN109486689B (zh) * 2018-12-27 2020-06-16 江南大学 一种增强l-天冬酰胺酶耐酸性的方法
CN110117631A (zh) * 2019-05-24 2019-08-13 山东大学 一种利用三种糖基转移酶制备Globotriose-连接的活性糖肽的方法

Also Published As

Publication number Publication date
AU2016363711A9 (en) 2018-12-13
WO2017093291A9 (en) 2018-07-12
US20180354997A1 (en) 2018-12-13
US20220242919A1 (en) 2022-08-04
AU2016363711A1 (en) 2018-06-14
EP3384018A1 (en) 2018-10-10
US11236136B2 (en) 2022-02-01
WO2017093291A1 (en) 2017-06-08
JP7100584B2 (ja) 2022-07-13
CA3006400A1 (en) 2017-06-08
JP2019502401A (ja) 2019-01-31

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Application publication date: 20181109