JP7022453B2 - フィコシアニンの精製方法 - Google Patents
フィコシアニンの精製方法 Download PDFInfo
- Publication number
- JP7022453B2 JP7022453B2 JP2020509094A JP2020509094A JP7022453B2 JP 7022453 B2 JP7022453 B2 JP 7022453B2 JP 2020509094 A JP2020509094 A JP 2020509094A JP 2020509094 A JP2020509094 A JP 2020509094A JP 7022453 B2 JP7022453 B2 JP 7022453B2
- Authority
- JP
- Japan
- Prior art keywords
- phycocyanin
- solution
- weight
- peg
- phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 108010053210 Phycocyanin Proteins 0.000 title claims description 92
- 238000000034 method Methods 0.000 title claims description 47
- 238000000746 purification Methods 0.000 title description 2
- 239000012071 phase Substances 0.000 claims description 46
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 37
- 239000002202 Polyethylene glycol Substances 0.000 claims description 34
- 229920001223 polyethylene glycol Polymers 0.000 claims description 34
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 33
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 33
- 150000001720 carbohydrates Chemical class 0.000 claims description 28
- 235000014633 carbohydrates Nutrition 0.000 claims description 28
- 239000002028 Biomass Substances 0.000 claims description 19
- 238000001556 precipitation Methods 0.000 claims description 19
- 239000002244 precipitate Substances 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 14
- 241000192700 Cyanobacteria Species 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 9
- 239000008346 aqueous phase Substances 0.000 claims description 8
- NBGXQZRRLOGAJF-UHFFFAOYSA-N Maltulose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)(CO)OCC1O NBGXQZRRLOGAJF-UHFFFAOYSA-N 0.000 claims description 7
- JCQLYHFGKNRPGE-FCVZTGTOSA-N lactulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-FCVZTGTOSA-N 0.000 claims description 7
- 229960000511 lactulose Drugs 0.000 claims description 7
- PFCRQPBOOFTZGQ-UHFFFAOYSA-N lactulose keto form Natural products OCC(=O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O PFCRQPBOOFTZGQ-UHFFFAOYSA-N 0.000 claims description 7
- JCQLYHFGKNRPGE-HFZVAGMNSA-N maltulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-HFZVAGMNSA-N 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 241000195493 Cryptophyta Species 0.000 claims description 6
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 6
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 6
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 5
- 238000011084 recovery Methods 0.000 claims description 5
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 4
- 240000002900 Arthrospira platensis Species 0.000 claims description 4
- 229940082787 spirulina Drugs 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 claims description 3
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims description 3
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 claims description 3
- 229920002307 Dextran Polymers 0.000 claims description 3
- 229930091371 Fructose Natural products 0.000 claims description 3
- 239000005715 Fructose Substances 0.000 claims description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 claims description 3
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 claims description 3
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims description 3
- 229930182830 galactose Natural products 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 41
- 239000001166 ammonium sulphate Substances 0.000 description 11
- 238000005119 centrifugation Methods 0.000 description 8
- 238000001914 filtration Methods 0.000 description 8
- 229920002774 Maltodextrin Polymers 0.000 description 7
- 239000005913 Maltodextrin Substances 0.000 description 7
- 238000011109 contamination Methods 0.000 description 7
- 229940035034 maltodextrin Drugs 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 230000005484 gravity Effects 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 239000011324 bead Substances 0.000 description 4
- 239000002537 cosmetic Substances 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- -1 R-phycocyanin Proteins 0.000 description 2
- 108010004469 allophycocyanin Proteins 0.000 description 2
- 238000009295 crossflow filtration Methods 0.000 description 2
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000013341 scale-up Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000005292 vacuum distillation Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 229920002245 Dextrose equivalent Polymers 0.000 description 1
- 239000008118 PEG 6000 Substances 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
- 229920002586 Polyethylene Glycol 7000 Polymers 0.000 description 1
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000011097 chromatography purification Methods 0.000 description 1
- 150000001860 citric acid derivatives Chemical class 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 230000002434 immunopotentiative effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/303—Extraction; Separation; Purification by precipitation by salting out
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1713293.7 | 2017-08-18 | ||
| GB1713293.7A GB2565591B (en) | 2017-08-18 | 2017-08-18 | Method of purifying phycocyanin |
| PCT/IB2018/055809 WO2019034955A1 (en) | 2017-08-18 | 2018-08-02 | PROCESS FOR PURIFYING PHYCOCYANINE |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020531476A JP2020531476A (ja) | 2020-11-05 |
| JP2020531476A5 JP2020531476A5 (https=) | 2021-09-09 |
| JP7022453B2 true JP7022453B2 (ja) | 2022-02-18 |
Family
ID=59996832
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020509094A Active JP7022453B2 (ja) | 2017-08-18 | 2018-08-02 | フィコシアニンの精製方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11795194B2 (https=) |
| EP (1) | EP3668885B1 (https=) |
| JP (1) | JP7022453B2 (https=) |
| ES (1) | ES2922323T3 (https=) |
| GB (1) | GB2565591B (https=) |
| WO (1) | WO2019034955A1 (https=) |
| ZA (1) | ZA202001585B (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3091703B1 (fr) * | 2019-01-11 | 2021-02-12 | Fermentalg | Procédé d’extraction de phycocyanines |
| CN113278528A (zh) * | 2021-06-30 | 2021-08-20 | 珠海康龙源进出口有限公司 | 一种螺旋藻破壁方法 |
| WO2024206398A1 (en) * | 2023-03-28 | 2024-10-03 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for extracting and encapsulating phycocyanin |
| CN118063632B (zh) * | 2023-11-29 | 2025-04-01 | 江南大学 | 一种麦芽糊精的制备方法 |
| CN119285756B (zh) * | 2024-10-22 | 2025-10-10 | 华南理工大学 | 一种低共熔双水相萃取钝顶螺旋藻藻蓝蛋白的分离方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106380513A (zh) | 2016-11-30 | 2017-02-08 | 江南大学 | 一种聚乙烯醚软脂酸双水相萃取螺旋藻藻蓝蛋白的分离技术 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4645829A (en) * | 1984-10-29 | 1987-02-24 | Monsanto Company | Method for separating polypeptides |
| IN182005B (https=) | 1993-06-15 | 1998-12-05 | Rieter Ag Maschf | |
| CN102993297B (zh) * | 2012-11-28 | 2014-04-30 | 汕头大学 | 一种螺旋藻藻蓝蛋白及其提取方法 |
-
2017
- 2017-08-18 GB GB1713293.7A patent/GB2565591B/en active Active
-
2018
- 2018-08-02 EP EP18762629.6A patent/EP3668885B1/en active Active
- 2018-08-02 JP JP2020509094A patent/JP7022453B2/ja active Active
- 2018-08-02 WO PCT/IB2018/055809 patent/WO2019034955A1/en not_active Ceased
- 2018-08-02 ES ES18762629T patent/ES2922323T3/es active Active
- 2018-08-02 US US16/639,250 patent/US11795194B2/en active Active
-
2020
- 2020-03-13 ZA ZA2020/01585A patent/ZA202001585B/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106380513A (zh) | 2016-11-30 | 2017-02-08 | 江南大学 | 一种聚乙烯醚软脂酸双水相萃取螺旋藻藻蓝蛋白的分离技术 |
Non-Patent Citations (2)
| Title |
|---|
| Biochemical Engineering Journal，2007年，Vol.34，pp.156-164 |
| Journal of Chemical Technology and Biotechnology，2001年，Vol.76，pp.1273-1280 |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2565591B (en) | 2020-02-26 |
| ZA202001585B (en) | 2021-04-28 |
| ES2922323T3 (es) | 2022-09-13 |
| EP3668885B1 (en) | 2022-03-02 |
| JP2020531476A (ja) | 2020-11-05 |
| WO2019034955A1 (en) | 2019-02-21 |
| GB2565591A (en) | 2019-02-20 |
| US11795194B2 (en) | 2023-10-24 |
| US20200255473A1 (en) | 2020-08-13 |
| EP3668885A1 (en) | 2020-06-24 |
| GB201713293D0 (en) | 2017-10-04 |
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