GB2565591B - Method of purifying phycocyanin - Google Patents

Method of purifying phycocyanin Download PDF

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Publication number
GB2565591B
GB2565591B GB1713293.7A GB201713293A GB2565591B GB 2565591 B GB2565591 B GB 2565591B GB 201713293 A GB201713293 A GB 201713293A GB 2565591 B GB2565591 B GB 2565591B
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GB
United Kingdom
Prior art keywords
phycocyanin
purifying
purifying phycocyanin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB1713293.7A
Other languages
English (en)
Other versions
GB2565591A (en
GB201713293D0 (en
Inventor
Therese Largier Harrison Susan
Armstrong Burke Matthew
William Mcclelland Pott Robert
Antonia Fagan-Endres Marijke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Cape Town
Original Assignee
University of Cape Town
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Cape Town filed Critical University of Cape Town
Priority to GB1713293.7A priority Critical patent/GB2565591B/en
Publication of GB201713293D0 publication Critical patent/GB201713293D0/en
Priority to EP18762629.6A priority patent/EP3668885B1/en
Priority to JP2020509094A priority patent/JP7022453B2/ja
Priority to PCT/IB2018/055809 priority patent/WO2019034955A1/en
Priority to ES18762629T priority patent/ES2922323T3/es
Priority to US16/639,250 priority patent/US11795194B2/en
Publication of GB2565591A publication Critical patent/GB2565591A/en
Application granted granted Critical
Publication of GB2565591B publication Critical patent/GB2565591B/en
Priority to ZA2020/01585A priority patent/ZA202001585B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/303—Extraction; Separation; Purification by precipitation by salting out
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
GB1713293.7A 2017-08-18 2017-08-18 Method of purifying phycocyanin Active GB2565591B (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
GB1713293.7A GB2565591B (en) 2017-08-18 2017-08-18 Method of purifying phycocyanin
EP18762629.6A EP3668885B1 (en) 2017-08-18 2018-08-02 Method of purifying phycocyanin
JP2020509094A JP7022453B2 (ja) 2017-08-18 2018-08-02 フィコシアニンの精製方法
PCT/IB2018/055809 WO2019034955A1 (en) 2017-08-18 2018-08-02 PROCESS FOR PURIFYING PHYCOCYANINE
ES18762629T ES2922323T3 (es) 2017-08-18 2018-08-02 Método de purificar ficocianina
US16/639,250 US11795194B2 (en) 2017-08-18 2018-08-02 Method of purifying phycocyanin
ZA2020/01585A ZA202001585B (en) 2017-08-18 2020-03-13 Method of purifying phycocyanin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1713293.7A GB2565591B (en) 2017-08-18 2017-08-18 Method of purifying phycocyanin

Publications (3)

Publication Number Publication Date
GB201713293D0 GB201713293D0 (en) 2017-10-04
GB2565591A GB2565591A (en) 2019-02-20
GB2565591B true GB2565591B (en) 2020-02-26

Family

ID=59996832

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1713293.7A Active GB2565591B (en) 2017-08-18 2017-08-18 Method of purifying phycocyanin

Country Status (7)

Country Link
US (1) US11795194B2 (https=)
EP (1) EP3668885B1 (https=)
JP (1) JP7022453B2 (https=)
ES (1) ES2922323T3 (https=)
GB (1) GB2565591B (https=)
WO (1) WO2019034955A1 (https=)
ZA (1) ZA202001585B (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3091703B1 (fr) * 2019-01-11 2021-02-12 Fermentalg Procédé d’extraction de phycocyanines
CN113278528A (zh) * 2021-06-30 2021-08-20 珠海康龙源进出口有限公司 一种螺旋藻破壁方法
WO2024206398A1 (en) * 2023-03-28 2024-10-03 Arizona Board Of Regents On Behalf Of Arizona State University Methods for extracting and encapsulating phycocyanin
CN118063632B (zh) * 2023-11-29 2025-04-01 江南大学 一种麦芽糊精的制备方法
CN119285756B (zh) * 2024-10-22 2025-10-10 华南理工大学 一种低共熔双水相萃取钝顶螺旋藻藻蓝蛋白的分离方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0180567A2 (en) * 1984-10-29 1986-05-07 Monsanto Company Method for separating polypeptides
CN102993297A (zh) * 2012-11-28 2013-03-27 汕头大学 一种螺旋藻藻蓝蛋白及其提取方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN182005B (https=) 1993-06-15 1998-12-05 Rieter Ag Maschf
CN106380513A (zh) 2016-11-30 2017-02-08 江南大学 一种聚乙烯醚软脂酸双水相萃取螺旋藻藻蓝蛋白的分离技术

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0180567A2 (en) * 1984-10-29 1986-05-07 Monsanto Company Method for separating polypeptides
CN102993297A (zh) * 2012-11-28 2013-03-27 汕头大学 一种螺旋藻藻蓝蛋白及其提取方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Biochemical Engineering Journal (2007); Vol 34, pp 156-164, "Aqueous two phase extraction for purification of C-phycocyanin" *
Biotechnology Techniques (1988); Vol 2, pp 277-282, "A low-cost aqueous two phase system for enzyme extraction", Szlag & Giuliano *
Journal of Chemical Technology & Biotechnology (2001); Vol 76, pp 1273-1289, "Practical application of aqueous two-phase systems...", Rito-Palomares et al *

Also Published As

Publication number Publication date
ZA202001585B (en) 2021-04-28
ES2922323T3 (es) 2022-09-13
EP3668885B1 (en) 2022-03-02
JP2020531476A (ja) 2020-11-05
WO2019034955A1 (en) 2019-02-21
GB2565591A (en) 2019-02-20
US11795194B2 (en) 2023-10-24
US20200255473A1 (en) 2020-08-13
EP3668885A1 (en) 2020-06-24
JP7022453B2 (ja) 2022-02-18
GB201713293D0 (en) 2017-10-04

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