JP6997772B2 - 核酸サンプル調製の方法 - Google Patents

核酸サンプル調製の方法 Download PDF

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JP6997772B2
JP6997772B2 JP2019514776A JP2019514776A JP6997772B2 JP 6997772 B2 JP6997772 B2 JP 6997772B2 JP 2019514776 A JP2019514776 A JP 2019514776A JP 2019514776 A JP2019514776 A JP 2019514776A JP 6997772 B2 JP6997772 B2 JP 6997772B2
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nucleic acid
adapter
target
primer
double
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JP2019531072A (ja
JP2019531072A5 (enExample
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ジョシュア スタール，
ジェイソン マイヤーズ，
ブレイディ カルバー，
ブライアン クドロー，
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アーチャーディーエックス， エルエルシー
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2019514776A 2016-09-15 2017-09-15 核酸サンプル調製の方法 Active JP6997772B2 (ja)

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US201662395339P 2016-09-15 2016-09-15
US62/395,339 2016-09-15
PCT/US2017/051924 WO2018053362A1 (en) 2016-09-15 2017-09-15 Methods of nucleic acid sample preparation

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JP2019531072A JP2019531072A (ja) 2019-10-31
JP2019531072A5 JP2019531072A5 (enExample) 2021-11-11
JP6997772B2 true JP6997772B2 (ja) 2022-01-18

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US (2) US10704082B2 (enExample)
EP (2) EP3512947B1 (enExample)
JP (1) JP6997772B2 (enExample)
CN (1) CN109937254B (enExample)
AU (1) AU2017328950B2 (enExample)
CA (1) CA3037185A1 (enExample)
ES (1) ES2903357T3 (enExample)
WO (1) WO2018053362A1 (enExample)

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CA2938080A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
US10704082B2 (en) 2016-09-15 2020-07-07 ArcherDX, Inc. Methods of nucleic acid sample preparation
JP6997773B2 (ja) * 2016-09-15 2022-01-18 アーチャーディーエックス， エルエルシー 無細胞ｄｎａの分析用の核酸サンプル調製の方法
US20220282305A1 (en) * 2016-09-15 2022-09-08 Archerdx, Llc Methods of nucleic acid sample preparation
JP7161991B2 (ja) * 2016-11-02 2022-10-27 アーチャーディーエックス， エルエルシー 免疫レパートリーシーケンシングのための核酸サンプル調製の方法
EP3972611A4 (en) * 2019-05-21 2023-06-14 Academia Sinica METHOD FOR AMPLIFICATION AND DETECTION OF RIBONUCLEIC ACID (RNA) FRAGMENTS
EP4012029B1 (en) * 2019-08-09 2024-05-29 GeneMind Biosciences Company Limited Method for capturing nucleic acid molecule, preparation method for nucleic acid library, and a sequencing method
CN114929895A (zh) * 2019-11-05 2022-08-19 阿尔缇玛基因组学公司 用于制备多核苷酸分子文库的方法
EP4069867B1 (en) * 2019-12-04 2025-08-27 Becton, Dickinson and Company Magnetic capture bead mediated molecular barcoding of nucleic acid targets in single particles and compositions for use in the same
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WO2022146773A1 (en) * 2020-12-29 2022-07-07 Nuprobe Usa, Inc. Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta)
BR112023024014A2 (pt) 2021-05-19 2024-02-06 Seqstant Gmbh Método para análise de sequência em tempo real de fragmentos de dna, oligonucleotídeo adaptador e kit para sequenciamento paralelo em tempo real e uso destes
WO2024055320A1 (zh) * 2022-09-16 2024-03-21 中国疾病预防控制中心传染病预防控制所 一种基因测序方法、装置、设备和介质
CN118028444A (zh) * 2022-09-16 2024-05-14 中国疾病预防控制中心传染病预防控制所 一种基因测序方法、装置、设备和介质
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