JP6968468B2 - 組換えタンパク質の製造方法 - Google Patents

組換えタンパク質の製造方法 Download PDF

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Publication number
JP6968468B2
JP6968468B2 JP2020560429A JP2020560429A JP6968468B2 JP 6968468 B2 JP6968468 B2 JP 6968468B2 JP 2020560429 A JP2020560429 A JP 2020560429A JP 2020560429 A JP2020560429 A JP 2020560429A JP 6968468 B2 JP6968468 B2 JP 6968468B2
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protein
recombinant
cell
spiders
cells
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Japanese (ja)
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JPWO2020158877A1 (ja
Inventor
憲治 中東
李 蒋
昂文 野田
研二 津山
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Spiber Inc
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Spiber Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020560429A 2019-01-31 2020-01-30 組換えタンパク質の製造方法 Active JP6968468B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2021069163A JP7526489B2 (ja) 2019-01-31 2021-04-15 組換えタンパク質の製造方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2019015716 2019-01-31
JP2019015716 2019-01-31
JP2019142388 2019-08-01
JP2019142388 2019-08-01
PCT/JP2020/003487 WO2020158877A1 (ja) 2019-01-31 2020-01-30 組換えタンパク質の製造方法

Related Child Applications (1)

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JP2021069163A Division JP7526489B2 (ja) 2019-01-31 2021-04-15 組換えタンパク質の製造方法

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JPWO2020158877A1 JPWO2020158877A1 (ja) 2021-02-18
JP6968468B2 true JP6968468B2 (ja) 2021-11-17

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JP2021069163A Active JP7526489B2 (ja) 2019-01-31 2021-04-15 組換えタンパク質の製造方法

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Country Status (8)

Country Link
US (1) US20220033870A1 (OSRAM)
EP (1) EP3919506A4 (OSRAM)
JP (2) JP6968468B2 (OSRAM)
CN (1) CN113439087A (OSRAM)
BR (1) BR112021014805A2 (OSRAM)
CA (1) CA3127177A1 (OSRAM)
SG (1) SG11202107697UA (OSRAM)
WO (1) WO2020158877A1 (OSRAM)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021020546A1 (ja) * 2019-08-01 2021-02-04 Ｓｐｉｂｅｒ株式会社 組換え細胞及び組換えタンパク質の製造方法
EP4476356A1 (en) * 2022-03-24 2024-12-18 Richter Gedeon Nyrt. Method for the manufacture of biopharmaceuticals

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58110600A (ja) 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JPS60221091A (ja) 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
JP2564268B2 (ja) 1985-08-28 1996-12-18 協和醗酵工業株式会社 融合抗原ポリペプチド
JP2545078B2 (ja) 1987-04-06 1996-10-16 協和醗酵工業株式会社 核酸関連物質の製造法
EP0326046A3 (en) 1988-01-25 1990-06-13 Takeda Chemical Industries, Ltd. Production of human epidermal growth factor
JPH0322979A (ja) 1989-06-19 1991-01-31 Kyowa Hakko Kogyo Co Ltd 新規プラスミノーゲン活性化因子
JP3012026B2 (ja) 1991-03-05 2000-02-21 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクター を保有する微生物並びに該微生物を 用いる有用物質の製造法
JP3433807B2 (ja) 1992-03-31 2003-08-04 鵜高 重三 新規アミノ酸配列及び該アミノ酸配列をコードするｄｎａを保有する発現ベクターを組み込んだバチルス・ブレビスを用いる有用物質の製造法
JP2727391B2 (ja) 1992-07-23 1998-03-11 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクターを保有する微生物並びに該微生物を用いる有用物質の製造法
JP2771756B2 (ja) 1993-04-09 1998-07-02 東洋エンジニアリング株式会社 組換え大腸菌の培養方法
CA2360085A1 (en) * 1999-01-27 2000-08-03 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in escherichia coli
JP3753945B2 (ja) 2001-02-14 2006-03-08 ヒゲタ醤油株式会社 大腸菌とブレビバチルス属細菌間のプラスミドシャトルベクター
KR100689932B1 (ko) * 2002-03-06 2007-03-12 도레이 가부시끼가이샤 유전자 재조합 누에를 이용한 생리 활성 단백질 생산법
KR100499647B1 (ko) * 2002-09-25 2005-07-05 한국과학기술원 박테리아에서의 외래단백질의 생산성을 증가시키는 방법
US8232377B2 (en) * 2006-05-16 2012-07-31 National Institute Of Advanced Industrial Science And Technology Method for high-level secretory production of protein
BRPI0701826B1 (pt) * 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira
DK3147369T3 (da) * 2014-05-21 2025-09-08 Spiber Inc Fremgangsmåde til produktion af fibroinlignende protein
CN105331568B (zh) * 2014-06-12 2019-01-04 清华大学 一种通过增大细菌体积而增加微生物胞内内含物积累量的方法
CN109563531A (zh) * 2016-08-02 2019-04-02 丝芭博株式会社 重组蛋白的生产方法

Also Published As

Publication number Publication date
CN113439087A (zh) 2021-09-24
BR112021014805A2 (pt) 2022-01-04
JP7526489B2 (ja) 2024-08-01
JPWO2020158877A1 (ja) 2021-02-18
EP3919506A4 (en) 2022-11-02
US20220033870A1 (en) 2022-02-03
CA3127177A1 (en) 2020-08-06
WO2020158877A1 (ja) 2020-08-06
EP3919506A1 (en) 2021-12-08
SG11202107697UA (en) 2021-08-30
JP2021101744A (ja) 2021-07-15

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