CA3127177A1 - Method for producing recombinant protein - Google Patents

Method for producing recombinant protein Download PDF

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Publication number
CA3127177A1
CA3127177A1 CA3127177A CA3127177A CA3127177A1 CA 3127177 A1 CA3127177 A1 CA 3127177A1 CA 3127177 A CA3127177 A CA 3127177A CA 3127177 A CA3127177 A CA 3127177A CA 3127177 A1 CA3127177 A1 CA 3127177A1
Authority
CA
Canada
Prior art keywords
protein
recombinant
cells
recombinant cells
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3127177A
Other languages
English (en)
French (fr)
Inventor
Kenji Nakahigashi
Li Jiang
Takanobu Noda
Kenji Tsuyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spiber Inc
Original Assignee
Spiber Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spiber Inc filed Critical Spiber Inc
Publication of CA3127177A1 publication Critical patent/CA3127177A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3127177A 2019-01-31 2020-01-30 Method for producing recombinant protein Pending CA3127177A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2019-015716 2019-01-31
JP2019015716 2019-01-31
JP2019-142388 2019-08-01
JP2019142388 2019-08-01
PCT/JP2020/003487 WO2020158877A1 (ja) 2019-01-31 2020-01-30 組換えタンパク質の製造方法

Publications (1)

Publication Number Publication Date
CA3127177A1 true CA3127177A1 (en) 2020-08-06

Family

ID=71840352

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3127177A Pending CA3127177A1 (en) 2019-01-31 2020-01-30 Method for producing recombinant protein

Country Status (8)

Country Link
US (1) US20220033870A1 (OSRAM)
EP (1) EP3919506A4 (OSRAM)
JP (2) JP6968468B2 (OSRAM)
CN (1) CN113439087A (OSRAM)
BR (1) BR112021014805A2 (OSRAM)
CA (1) CA3127177A1 (OSRAM)
SG (1) SG11202107697UA (OSRAM)
WO (1) WO2020158877A1 (OSRAM)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021020546A1 (ja) * 2019-08-01 2021-02-04 Ｓｐｉｂｅｒ株式会社 組換え細胞及び組換えタンパク質の製造方法
EP4476356A1 (en) * 2022-03-24 2024-12-18 Richter Gedeon Nyrt. Method for the manufacture of biopharmaceuticals

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58110600A (ja) 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JPS60221091A (ja) 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
JP2564268B2 (ja) 1985-08-28 1996-12-18 協和醗酵工業株式会社 融合抗原ポリペプチド
JP2545078B2 (ja) 1987-04-06 1996-10-16 協和醗酵工業株式会社 核酸関連物質の製造法
EP0326046A3 (en) 1988-01-25 1990-06-13 Takeda Chemical Industries, Ltd. Production of human epidermal growth factor
JPH0322979A (ja) 1989-06-19 1991-01-31 Kyowa Hakko Kogyo Co Ltd 新規プラスミノーゲン活性化因子
JP3012026B2 (ja) 1991-03-05 2000-02-21 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクター を保有する微生物並びに該微生物を 用いる有用物質の製造法
JP3433807B2 (ja) 1992-03-31 2003-08-04 鵜高 重三 新規アミノ酸配列及び該アミノ酸配列をコードするｄｎａを保有する発現ベクターを組み込んだバチルス・ブレビスを用いる有用物質の製造法
JP2727391B2 (ja) 1992-07-23 1998-03-11 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクターを保有する微生物並びに該微生物を用いる有用物質の製造法
JP2771756B2 (ja) 1993-04-09 1998-07-02 東洋エンジニアリング株式会社 組換え大腸菌の培養方法
CA2360085A1 (en) * 1999-01-27 2000-08-03 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in escherichia coli
JP3753945B2 (ja) 2001-02-14 2006-03-08 ヒゲタ醤油株式会社 大腸菌とブレビバチルス属細菌間のプラスミドシャトルベクター
KR100689932B1 (ko) * 2002-03-06 2007-03-12 도레이 가부시끼가이샤 유전자 재조합 누에를 이용한 생리 활성 단백질 생산법
KR100499647B1 (ko) * 2002-09-25 2005-07-05 한국과학기술원 박테리아에서의 외래단백질의 생산성을 증가시키는 방법
US8232377B2 (en) * 2006-05-16 2012-07-31 National Institute Of Advanced Industrial Science And Technology Method for high-level secretory production of protein
BRPI0701826B1 (pt) * 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira
DK3147369T3 (da) * 2014-05-21 2025-09-08 Spiber Inc Fremgangsmåde til produktion af fibroinlignende protein
CN105331568B (zh) * 2014-06-12 2019-01-04 清华大学 一种通过增大细菌体积而增加微生物胞内内含物积累量的方法
CN109563531A (zh) * 2016-08-02 2019-04-02 丝芭博株式会社 重组蛋白的生产方法

Also Published As

Publication number Publication date
JP6968468B2 (ja) 2021-11-17
CN113439087A (zh) 2021-09-24
BR112021014805A2 (pt) 2022-01-04
JP7526489B2 (ja) 2024-08-01
JPWO2020158877A1 (ja) 2021-02-18
EP3919506A4 (en) 2022-11-02
US20220033870A1 (en) 2022-02-03
WO2020158877A1 (ja) 2020-08-06
EP3919506A1 (en) 2021-12-08
SG11202107697UA (en) 2021-08-30
JP2021101744A (ja) 2021-07-15

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