JP6849868B2 - シングルアーム一価抗体構築物およびその用途 - Google Patents

シングルアーム一価抗体構築物およびその用途 Download PDF

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JP6849868B2
JP6849868B2 JP2015510590A JP2015510590A JP6849868B2 JP 6849868 B2 JP6849868 B2 JP 6849868B2 JP 2015510590 A JP2015510590 A JP 2015510590A JP 2015510590 A JP2015510590 A JP 2015510590A JP 6849868 B2 JP6849868 B2 JP 6849868B2
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polypeptide
construct
her2
binding
antibody construct
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JP2015522526A (ja
JP2015522526A5 (enExample
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ゴードン イウ コン エング
ゴードン イウ コン エング
フォン クロイデンシュタイン トマス スプレッター
フォン クロイデンシュタイン トマス スプレッター
ニナ イー． ヴァイサー
ニナ イー． ヴァイサー
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ザイムワークス，インコーポレイテッド
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    • C07—ORGANIC CHEMISTRY
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
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    • C07—ORGANIC CHEMISTRY
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
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    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/39558—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against tumor tissues, cells, antigens
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
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    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
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    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
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JP2015510590A 2012-05-10 2013-05-08 シングルアーム一価抗体構築物およびその用途 Expired - Fee Related JP6849868B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201261645547P 2012-05-10 2012-05-10
US61/645,547 2012-05-10
US201261671640P 2012-07-13 2012-07-13
US61/671,640 2012-07-13
US201261722070P 2012-11-02 2012-11-02
US61/722,070 2012-11-02
US201361762812P 2013-02-08 2013-02-08
US61/762,812 2013-02-08
PCT/CA2013/050358 WO2013166604A1 (en) 2012-05-10 2013-05-08 Single-arm monovalent antibody constructs and uses thereof

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JP2015522526A JP2015522526A (ja) 2015-08-06
JP2015522526A5 JP2015522526A5 (enExample) 2017-11-02
JP6849868B2 true JP6849868B2 (ja) 2021-03-31

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US (2) US20150125449A1 (enExample)
EP (1) EP2847224A4 (enExample)
JP (1) JP6849868B2 (enExample)
KR (1) KR20150008171A (enExample)
CN (1) CN104520327B (enExample)
AU (1) AU2013258844B2 (enExample)
CA (1) CA2873720A1 (enExample)
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Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011325833C1 (en) 2010-11-05 2017-07-13 Zymeworks Bc Inc. Stable heterodimeric antibody design with mutations in the Fc domain
ES2676878T3 (es) 2011-03-03 2018-07-25 Zymeworks Inc. Diseño de armazón de heteromultímero multivalente y constructos
AU2012332021B8 (en) 2011-11-04 2017-10-12 Zymeworks Bc Inc. Stable heterodimeric antibody design with mutations in the Fc domain
WO2014067011A1 (en) * 2012-11-02 2014-05-08 Zymeworks Inc. Crystal structures of heterodimeric fc domains
SG10201709018QA (en) 2012-03-14 2017-11-29 Regeneron Pharma Multispecific antigen-binding molecules and uses thereof
US9499634B2 (en) 2012-06-25 2016-11-22 Zymeworks Inc. Process and methods for efficient manufacturing of highly pure asymmetric antibodies in mammalian cells
CA2878640C (en) 2012-07-13 2023-10-24 Zymeworks Inc. Multivalent heteromultimer scaffold design and constructs
US9914785B2 (en) 2012-11-28 2018-03-13 Zymeworks Inc. Engineered immunoglobulin heavy chain-light chain pairs and uses thereof
EA037325B1 (ru) 2013-07-09 2021-03-12 Аннексон, Инк. Антитела против фактора комплемента c1q и их применения
US10273303B2 (en) * 2013-11-13 2019-04-30 Zymeworks Inc. Monovalent antigen binding constructs targeting EGFR and/or HER2 and uses thereof
WO2015077891A1 (en) * 2013-11-27 2015-06-04 Zymeworks Inc. Bispecific antigen-binding constructs targeting her2
WO2016082044A1 (en) 2014-11-27 2016-06-02 Zymeworks Inc. Methods of using bispecific antigen-binding constructs targeting her2
EP3514181A1 (en) * 2015-02-24 2019-07-24 Academia Sinica A phage-displayed single-chain variable fragment library and antibodies selected therefrom
EP3294772B1 (en) * 2015-05-13 2025-11-05 Zymeworks BC Inc. Antigen-binding constructs targeting her2
AU2016289480C1 (en) 2015-07-06 2021-10-21 Regeneron Pharmaceuticals, Inc. Multispecific antigen-binding molecules and uses thereof
CN108137706A (zh) 2015-07-15 2018-06-08 酵活有限公司 药物缀合的双特异性抗原结合构建体
US11014983B2 (en) 2015-08-20 2021-05-25 Sutro Biopharma, Inc. Anti-Tim-3 antibodies, compositions comprising anti-Tim-3 antibodies and methods of making and using anti-Tim-3 antibodies
IL296130A (en) 2015-11-24 2022-11-01 Annexon Inc Anti-complement factor c1q fab fragments and their uses
BR112018070676A2 (pt) * 2016-04-15 2019-02-05 Zymeworks Inc construtos de ligação a antígeno multiespecífico que têm como alvo agentes imunoterapêuticos
WO2017190079A1 (en) 2016-04-28 2017-11-02 Regeneron Pharmaceuticals, Inc. Methods of making multispecific antigen-binding molecules
WO2018156785A1 (en) * 2017-02-22 2018-08-30 Sutro Biopharma, Inc. Pd-1/tim-3 bi-specific antibodies, compositions thereof, and methods of making and using the same
JOP20190222A1 (ar) 2017-04-11 2019-09-24 Zymeworks Inc الأجسام المضادة ثنائية النوعية المضادة لـ pd-l1 والمضادة لـ tim-3
AR112603A1 (es) 2017-07-10 2019-11-20 Lilly Co Eli Anticuerpos biespecíficos inhibidores de punto de control
MX2020005981A (es) 2017-12-08 2020-08-24 Argenx Bvba Uso de antagonistas del fcrn para el tratamiento de la miastenia gravis generalizada.
JP7328990B2 (ja) 2018-04-30 2023-08-17 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｈｅｒ２及び／またはａｐｌｐ２に結合する抗体及び二重特異性抗原結合分子、ならびにそれらのコンジュゲート及び使用
CN112513073A (zh) 2018-06-08 2021-03-16 阿根思公司 用于治疗免疫性血小板减少症的组合物和方法
MY207066A (en) 2018-08-17 2025-01-28 Regeneron Pharma Method and chromatography system for determining amount and purity of a multimeric protein
US12403175B2 (en) 2020-01-08 2025-09-02 argenx BV Methods for treating pemphigus disorders
JP7651145B2 (ja) * 2021-04-20 2025-03-26 コリア ユニバーシティ リサーチ アンド ビジネス ファウンデーション 向上した癌細胞死滅効能を有する非対称抗体
US12486322B2 (en) 2021-12-13 2025-12-02 Annexon, Inc. Anti-complement factor C1q antibodies with single binding arms and uses thereof
WO2023242372A1 (en) 2022-06-15 2023-12-21 argenx BV Fcrn/hsa binding molecules and methods of use

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050073488A (ko) * 2002-10-08 2005-07-13 이뮤노메딕스, 인코오포레이티드 Ⅲ종 항-ｃｅａ 단클론 항체들 및 치료제들을 이용한 조합치료법
EP1718677B1 (en) * 2003-12-19 2012-04-18 Genentech, Inc. Monovalent antibody fragments useful as therapeutics
GB0614780D0 (en) * 2006-07-25 2006-09-06 Ucb Sa Biological products
WO2008131242A1 (en) * 2007-04-18 2008-10-30 Zymogenetics, Inc. Single chain fc, methods of making and methods of treatment
SI2235064T1 (sl) * 2008-01-07 2016-04-29 Amgen Inc. Metoda za izdelavo heterodimernih molekul - protitelesa fc z uporabo elektrostatičnih usmerjevalnih učinkov
AR070862A1 (es) * 2008-03-06 2010-05-12 Genentech Inc Terapia de combinacion con antagonistas de c- met y her
US20110262436A1 (en) * 2008-10-17 2011-10-27 Genentech, Inc. Treatment method
WO2011120135A1 (en) * 2010-03-29 2011-10-06 Zymeworks, Inc. Antibodies with enhanced or suppressed effector function
ES2989108T3 (es) * 2010-04-20 2024-11-25 Genmab As Proteínas que contienen FC de anticuerpos heterodiméricos y métodos para producir las mismas
CA2800769C (en) * 2010-05-27 2021-11-02 Genmab A/S Monoclonal antibodies against her2 epitope
TW201217527A (en) * 2010-07-09 2012-05-01 Biogen Idec Hemophilia Inc Processable single chain molecules and polypeptides made using same
AU2011325833C1 (en) * 2010-11-05 2017-07-13 Zymeworks Bc Inc. Stable heterodimeric antibody design with mutations in the Fc domain
US9738724B2 (en) * 2012-06-08 2017-08-22 Sutro Biopharma, Inc. Antibodies comprising site-specific non-natural amino acid residues, methods of their preparation and methods of their use
CA2929834A1 (en) * 2013-11-13 2015-05-21 Zymeworks Inc. Methods using monovalent antigen binding constructs targeting her2
JP6531010B2 (ja) * 2015-08-19 2019-06-12 本田技研工業株式会社 駆動装置、輸送機器及び蓄電器制御方法

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EP2847224A4 (en) 2016-04-27
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AU2013258844A1 (en) 2014-12-04
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