JP6842800B2 - 酵素的方法を使用してレバウディオサイドｊを調製するための方法 - Google Patents

酵素的方法を使用してレバウディオサイドｊを調製するための方法 Download PDF

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JP6842800B2
JP6842800B2 JP2019521072A JP2019521072A JP6842800B2 JP 6842800 B2 JP6842800 B2 JP 6842800B2 JP 2019521072 A JP2019521072 A JP 2019521072A JP 2019521072 A JP2019521072 A JP 2019521072A JP 6842800 B2 JP6842800 B2 JP 6842800B2
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rebaudioside
ugt
glycosyltransferase
udp
reaction
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JP2019536444A5 (https=
JP2019536444A (ja
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軍華 陶
軍華 陶
国慶 李
国慶 李
文霞 王
文霞 王
雷雷 鄭
雷雷 鄭
春磊 朱
春磊 朱
暁亮 梁
暁亮 梁
車翹 陳
車翹 陳
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ペプシコ・インク
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2019521072A 2016-10-21 2016-10-21 酵素的方法を使用してレバウディオサイドｊを調製するための方法 Active JP6842800B2 (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/102942 WO2018072211A1 (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙j的方法

Related Child Applications (1)

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JP2021024483A Division JP7210626B2 (ja) 2021-02-18 2021-02-18 酵素的方法を使用してレバウディオサイドｊを調製するための方法

Publications (3)

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JP2019536444A JP2019536444A (ja) 2019-12-19
JP2019536444A5 JP2019536444A5 (https=) 2021-01-14
JP6842800B2 true JP6842800B2 (ja) 2021-03-17

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JP2019521072A Active JP6842800B2 (ja) 2016-10-21 2016-10-21 酵素的方法を使用してレバウディオサイドｊを調製するための方法

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Country Link
US (3) US11359222B2 (https=)
EP (1) EP3543346A4 (https=)
JP (1) JP6842800B2 (https=)
CN (2) CN116574775A (https=)
AU (1) AU2016427128C1 (https=)
MX (1) MX2019004630A (https=)
RU (1) RU2736352C1 (https=)
WO (1) WO2018072211A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112019008048A2 (pt) 2016-10-21 2019-07-02 Pepsico Inc método para preparar rebaudiosídeo n com o uso de método enzimático
US11312985B2 (en) 2016-10-21 2022-04-26 Pepsico, Inc. Enzymatic method for preparing Rebaudioside C
CN111868252B (zh) 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产

Family Cites Families (29)

* Cited by examiner, † Cited by third party
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ES2381892T3 (es) 2007-01-22 2012-06-01 Cargill, Incorporated Procedimiento para producir composiciones de rebaudiósido A purificado que utiliza la cristalización disolvente/antidisolvente
CN101225424B (zh) 2007-09-13 2013-05-29 天津药物研究院 环黄芪醇的单葡萄糖苷、其制备方法、药物组合物和应用
ES2592377T3 (es) 2008-10-03 2016-11-29 Morita Kagaku Kogyo Co., Ltd. Nuevos glucósidos de esteviol
CA3015571C (en) 2009-10-15 2021-07-06 Purecircle Sdn Bhd High-purity rebaudioside d and applications
EP2575432B1 (en) 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
US8962698B2 (en) 2011-01-28 2015-02-24 Tate & Lyle Ingredients Americas Llc Rebaudioside-mogroside V blends
CA3128532A1 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
CN103031283B (zh) 2011-10-08 2015-07-08 成都华高瑞甜科技有限公司 甜叶菊酶vi及莱鲍迪苷a转化为莱鲍迪苷d的方法
CN103159808B (zh) 2011-12-09 2017-03-29 上海泓博智源医药股份有限公司 一种制备天然甜味剂的工艺方法
EP4124245A1 (en) 2011-12-19 2023-02-01 The Coca-Cola Company Beverage comprising rebaudioside x
MX355028B (es) 2012-01-23 2018-03-28 Dsm Ip Assets Bv Produccion de diterpeno.
US9752174B2 (en) 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
MX395285B (es) * 2012-05-22 2025-03-25 Purecircle Sdn Bhd Glucosidos de esteviol de alta pureza.
EP2928321A1 (en) 2012-12-05 2015-10-14 Evolva SA Steviol glycoside compositions sensory properties
CN103088041B (zh) 2013-01-29 2015-01-07 江南大学 一种可用于高效生产角质酶的角质酶基因及其应用
MY199452A (en) 2013-02-06 2023-10-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
US9717267B2 (en) * 2013-03-14 2017-08-01 The Coca-Cola Company Beverages containing rare sugars
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
SG2013092820A (en) 2013-12-16 2015-07-30 Ngee Ann Polytechnic Xylose isomerase genes for xylose-fermenting yeast construction
CN103757074B (zh) * 2014-01-16 2015-12-02 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
CN106471128A (zh) 2014-01-28 2017-03-01 百事可乐公司 一种酶法制备瑞鲍迪甙m的方法
US9522929B2 (en) * 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
EP3683315A1 (en) 2014-08-19 2020-07-22 Purecircle SDN BHD Method for preparing rebaudioside m
BR112017006163B1 (pt) 2014-10-03 2022-04-05 Conagen Inc Produto de consumo oral e método para adoçar o mesmo
US10612065B2 (en) 2015-05-29 2020-04-07 Cargill, Incorporated Heat treatment to produce glycosides
CN105200098A (zh) * 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法
US20190203244A1 (en) 2015-08-20 2019-07-04 Pepsico, Inc. Preparation of rebaudioside m in a single reaction vessel
US11312985B2 (en) 2016-10-21 2022-04-26 Pepsico, Inc. Enzymatic method for preparing Rebaudioside C
BR112019008048A2 (pt) 2016-10-21 2019-07-02 Pepsico Inc método para preparar rebaudiosídeo n com o uso de método enzimático

Also Published As

Publication number Publication date
CN116574775A (zh) 2023-08-11
EP3543346A1 (en) 2019-09-25
US11952604B2 (en) 2024-04-09
MX2019004630A (es) 2019-07-15
AU2016427128B2 (en) 2022-08-18
US20190338332A1 (en) 2019-11-07
AU2016427128C1 (en) 2023-01-05
US20230022453A1 (en) 2023-01-26
JP2019536444A (ja) 2019-12-19
WO2018072211A1 (zh) 2018-04-26
US11359222B2 (en) 2022-06-14
RU2736352C1 (ru) 2020-11-16
CN110177881A (zh) 2019-08-27
BR112019007882A2 (pt) 2019-07-02
CN110177881B (zh) 2023-06-02
AU2016427128A1 (en) 2019-05-30
EP3543346A4 (en) 2020-07-22
US20240287567A1 (en) 2024-08-29
CA3041147A1 (en) 2018-04-26

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