AU2016427128C1 - Method for preparing rebaudioside J using enzymatic method - Google Patents

Method for preparing rebaudioside J using enzymatic method Download PDF

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Publication number
AU2016427128C1
AU2016427128C1 AU2016427128A AU2016427128A AU2016427128C1 AU 2016427128 C1 AU2016427128 C1 AU 2016427128C1 AU 2016427128 A AU2016427128 A AU 2016427128A AU 2016427128 A AU2016427128 A AU 2016427128A AU 2016427128 C1 AU2016427128 C1 AU 2016427128C1
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Prior art keywords
rebaudioside
ala
leu
udp
ugt
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AU2016427128A
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AU2016427128B2 (en
AU2016427128A1 (en
Inventor
Kuikiu CHAN
Guoqing Li
Xiaoliang LIANG
Alex TAO
Wenxia WANG
Leilei ZHENG
Chunlei Zhu
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Pepsico Inc
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Pepsico Inc
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Assigned to PEPSICO, INC. reassignment PEPSICO, INC. Amend patent request/document other than specification (104) Assignors: PEPSICO INC.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2016427128A 2016-10-21 2016-10-21 Method for preparing rebaudioside J using enzymatic method Active AU2016427128C1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/102942 WO2018072211A1 (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙j的方法

Publications (3)

Publication Number Publication Date
AU2016427128A1 AU2016427128A1 (en) 2019-05-30
AU2016427128B2 AU2016427128B2 (en) 2022-08-18
AU2016427128C1 true AU2016427128C1 (en) 2023-01-05

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Family Applications (1)

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AU2016427128A Active AU2016427128C1 (en) 2016-10-21 2016-10-21 Method for preparing rebaudioside J using enzymatic method

Country Status (8)

Country Link
US (3) US11359222B2 (https=)
EP (1) EP3543346A4 (https=)
JP (1) JP6842800B2 (https=)
CN (2) CN110177881B (https=)
AU (1) AU2016427128C1 (https=)
MX (1) MX2019004630A (https=)
RU (1) RU2736352C1 (https=)
WO (1) WO2018072211A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016427120B2 (en) 2016-10-21 2022-08-18 Pepsico, Inc. Method for preparing rebaudioside C using enzymatic method
BR112019008048A2 (pt) 2016-10-21 2019-07-02 Pepsico Inc método para preparar rebaudiosídeo n com o uso de método enzimático
CN111868252B (zh) 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产

Citations (1)

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CN103757074A (zh) * 2014-01-16 2014-04-30 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法

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JP5307730B2 (ja) 2007-01-22 2013-10-02 カーギル・インコーポレイテッド 溶媒／貧溶媒晶析を用いる精製レバウディオサイドａ組成物の製法
CN101225424B (zh) 2007-09-13 2013-05-29 天津药物研究院 环黄芪醇的单葡萄糖苷、其制备方法、药物组合物和应用
CN107033200A (zh) 2008-10-03 2017-08-11 守田化学工业株式会社 新的甜菊醇糖苷
ES2457641T3 (es) 2009-10-15 2014-04-28 Purecircle Sdn Bhd Procedimiento para preparar rebaudiósido D de pureza elevada
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
US8962698B2 (en) 2011-01-28 2015-02-24 Tate & Lyle Ingredients Americas Llc Rebaudioside-mogroside V blends
CN108396044A (zh) 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
CN103031283B (zh) 2011-10-08 2015-07-08 成都华高瑞甜科技有限公司 甜叶菊酶vi及莱鲍迪苷a转化为莱鲍迪苷d的方法
CN103159808B (zh) 2011-12-09 2017-03-29 上海泓博智源医药股份有限公司 一种制备天然甜味剂的工艺方法
EP3009010B1 (en) 2011-12-19 2020-02-05 PureCircle SDN BHD Methods for purifying steviol glycosides
AU2013211605B2 (en) 2012-01-23 2017-04-06 Dsm Ip Assets B.V. Diterpene production
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
BR112014028819A2 (pt) 2012-05-22 2017-07-25 Purecircle Sdn Bhd glicosídeos de esteviol de pureza elevada
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ES2805014T3 (es) 2014-08-19 2021-02-10 Purecircle Sdn Bhd Método para preparar rebaudiósido I
DK3200615T3 (da) * 2014-10-03 2019-08-19 Conagen Inc Kaloriefrie sødestoffer og fremgangsmåder til syntetisering
JP2018516081A (ja) 2015-05-29 2018-06-21 カーギル・インコーポレイテッド 配糖体を産生するための熱処理
CN105200098A (zh) * 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法
US20190203244A1 (en) 2015-08-20 2019-07-04 Pepsico, Inc. Preparation of rebaudioside m in a single reaction vessel
BR112019008048A2 (pt) 2016-10-21 2019-07-02 Pepsico Inc método para preparar rebaudiosídeo n com o uso de método enzimático
AU2016427120B2 (en) 2016-10-21 2022-08-18 Pepsico, Inc. Method for preparing rebaudioside C using enzymatic method

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Also Published As

Publication number Publication date
CN110177881B (zh) 2023-06-02
EP3543346A4 (en) 2020-07-22
JP6842800B2 (ja) 2021-03-17
AU2016427128B2 (en) 2022-08-18
RU2736352C1 (ru) 2020-11-16
CN116574775A (zh) 2023-08-11
US11359222B2 (en) 2022-06-14
JP2019536444A (ja) 2019-12-19
US20240287567A1 (en) 2024-08-29
US20190338332A1 (en) 2019-11-07
CN110177881A (zh) 2019-08-27
MX2019004630A (es) 2019-07-15
CA3041147A1 (en) 2018-04-26
US11952604B2 (en) 2024-04-09
US20230022453A1 (en) 2023-01-26
AU2016427128A1 (en) 2019-05-30
WO2018072211A1 (zh) 2018-04-26
EP3543346A1 (en) 2019-09-25
BR112019007882A2 (pt) 2019-07-02

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DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 19 SEP 2022

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Owner name: PEPSICO, INC.

Free format text: FORMER NAME(S): PEPSICO INC.

DA3 Amendments made section 104

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FGA Letters patent sealed or granted (standard patent)