JP6811173B2 - 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列 - Google Patents

増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列 Download PDF

Info

Publication number
JP6811173B2
JP6811173B2 JP2017514343A JP2017514343A JP6811173B2 JP 6811173 B2 JP6811173 B2 JP 6811173B2 JP 2017514343 A JP2017514343 A JP 2017514343A JP 2017514343 A JP2017514343 A JP 2017514343A JP 6811173 B2 JP6811173 B2 JP 6811173B2
Authority
JP
Japan
Prior art keywords
cbr
polypeptide
pbi
luciferase
luciferin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017514343A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017529840A (ja
JP2017529840A5 (https=
Inventor
ランス ピー エンセル
ランス ピー エンセル
メアリー ピー ホール
メアリー ピー ホール
キース ヴィー ウッド
キース ヴィー ウッド
モニカ ジー ウッド
モニカ ジー ウッド
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of JP2017529840A publication Critical patent/JP2017529840A/ja
Publication of JP2017529840A5 publication Critical patent/JP2017529840A5/ja
Application granted granted Critical
Publication of JP6811173B2 publication Critical patent/JP6811173B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2017514343A 2014-09-11 2015-09-11 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列 Active JP6811173B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462049150P 2014-09-11 2014-09-11
US62/049,150 2014-09-11
PCT/US2015/049661 WO2016040788A1 (en) 2014-09-11 2015-09-11 Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020000374A Division JP7058677B2 (ja) 2014-09-11 2020-01-06 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列

Publications (3)

Publication Number Publication Date
JP2017529840A JP2017529840A (ja) 2017-10-12
JP2017529840A5 JP2017529840A5 (https=) 2018-10-18
JP6811173B2 true JP6811173B2 (ja) 2021-01-13

Family

ID=54186325

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2017514343A Active JP6811173B2 (ja) 2014-09-11 2015-09-11 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
JP2020000374A Active JP7058677B2 (ja) 2014-09-11 2020-01-06 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
JP2022010240A Active JP7443405B2 (ja) 2014-09-11 2022-01-26 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
JP2024024330A Active JP7780560B2 (ja) 2014-09-11 2024-02-21 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2020000374A Active JP7058677B2 (ja) 2014-09-11 2020-01-06 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
JP2022010240A Active JP7443405B2 (ja) 2014-09-11 2022-01-26 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
JP2024024330A Active JP7780560B2 (ja) 2014-09-11 2024-02-21 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列

Country Status (4)

Country Link
US (4) US9732373B2 (https=)
EP (1) EP3191600A1 (https=)
JP (4) JP6811173B2 (https=)
WO (1) WO2016040788A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
EP3578978B1 (en) * 2017-02-03 2022-07-13 Osaka University Device and determination system using same
EP3661932B1 (en) * 2017-08-04 2021-10-06 Promega Corporation Compositions and methods for stabilizing benzothiazole luciferin analogs
CN111315875B (zh) 2017-10-25 2024-02-20 豪夫迈·罗氏有限公司 改进修饰的/突变的细菌萤光素酶
WO2019236731A1 (en) 2018-06-05 2019-12-12 Promega Corporation Luciferase enzymes for use with thermostable luciferins in bioluminescent assays
JP2025522599A (ja) * 2022-06-30 2025-07-15 青島華大智造普恵科技有限公司 ルシフェラーゼ変異体及びその使用
CN118516381B (zh) * 2024-07-22 2024-11-01 北京剂泰医药科技有限公司 编码荧光素酶的mRNA及其应用

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
JPH088864B2 (ja) 1988-04-12 1996-01-31 キッコーマン株式会社 ルシフェラーゼ
US5049358A (en) 1988-09-30 1991-09-17 Miles Inc. Composition and test device for assaying for proteins
US5196524A (en) 1989-01-06 1993-03-23 Eli Lilly And Company Fusion reporter gene for bacterial luciferase
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5480789A (en) 1991-04-01 1996-01-02 Florigene Europe B.V. Genetically transformed rose plants and methods for their production
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
JP3048466B2 (ja) 1991-06-27 2000-06-05 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
GB9301118D0 (en) 1993-01-21 1993-03-10 Secr Defence Enzyme linked assays
CA2157476C (en) * 1994-01-03 2009-08-25 Keith V. Wood Mutant luciferases
US6387675B1 (en) 1994-01-03 2002-05-14 Promega Corporation Mutant luciferases
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
GB2301592B (en) 1994-03-23 1998-06-17 Secr Defence Luciferases
GB9501170D0 (en) 1994-03-23 1995-03-08 Secr Defence Luciferases
ATE215608T1 (de) 1994-07-13 2002-04-15 Secr Defence Brit Mikrobiologisches testverfahren und reagenzien
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
WO1996022376A1 (en) 1995-01-20 1996-07-25 The Secretary Of State For Defence Mutant luciferases
US5716851A (en) 1996-01-16 1998-02-10 Bayer Corporation Glass/cellulose as protein reagent
JPH09294600A (ja) 1996-04-26 1997-11-18 Kikkoman Corp 複数のプロモーター活性の測定法
JP2001504325A (ja) 1996-09-27 2001-04-03 マキシジェン，インコーポレイテッド 回帰的配列シャフリングおよび選択による遺伝子治療の最適化のための方法
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
US6074859A (en) 1997-07-08 2000-06-13 Kikkoman Corporation Mutant-type bioluminescent protein, and process for producing the mutant-type bioluminescent protein
EP2860252A1 (en) 1997-09-19 2015-04-15 Promega Corporation Thermostable luciferases and methods of production
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
NZ518413A (en) 1999-10-26 2004-11-26 Secr Defence Novel enzyme
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
US7879540B1 (en) * 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7083911B2 (en) 2001-02-16 2006-08-01 Promega Corporation Method for detection of ATP
US7268229B2 (en) * 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
WO2005035719A2 (en) 2003-10-03 2005-04-21 Board Of Control Of Michigan Technological University Modified luciferase
EP2308978B8 (en) * 2003-10-10 2015-04-08 Promega Corporation Luciferase biosensor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
EP2272972A1 (en) * 2005-05-31 2011-01-12 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
EP2004813B1 (en) * 2006-04-03 2015-06-03 Promega Corporation Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides
WO2009140662A1 (en) * 2008-05-15 2009-11-19 Illumina, Inc. Engineered luciferases
US8288559B2 (en) * 2008-08-18 2012-10-16 Promega Corporation Luminogenic compounds and methods to detect cytochrome P450 3A enzymes
ES2795287T3 (es) 2009-05-01 2020-11-23 Promega Corp Luciferasas de Oplophorus sintéticas con mayor emisión de luz
JP5644994B2 (ja) * 2009-08-31 2014-12-24 独立行政法人産業技術総合研究所 バイオイメージングに特化した変異型赤色発光ルシフェラーゼ
SG10202103336SA (en) 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
JP6495232B2 (ja) * 2013-03-12 2019-04-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 赤方偏移ルシフェリン及びその使用方法
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence

Also Published As

Publication number Publication date
EP3191600A1 (en) 2017-07-19
JP2017529840A (ja) 2017-10-12
US20170314061A1 (en) 2017-11-02
US20220186287A1 (en) 2022-06-16
US20160076079A1 (en) 2016-03-17
US20200115735A1 (en) 2020-04-16
WO2016040788A1 (en) 2016-03-17
JP7058677B2 (ja) 2022-04-22
JP2020099328A (ja) 2020-07-02
US12460245B2 (en) 2025-11-04
JP2022068170A (ja) 2022-05-09
JP7780560B2 (ja) 2025-12-04
US9732373B2 (en) 2017-08-15
JP7443405B2 (ja) 2024-03-05
US11293047B2 (en) 2022-04-05
JP2024073457A (ja) 2024-05-29
US10550420B2 (en) 2020-02-04

Similar Documents

Publication Publication Date Title
JP7058677B2 (ja) 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
Markova et al. Shining light on the secreted luciferases of marine copepods: Current knowledge and applications
JP5889910B2 (ja) 新規セレンテラジン基質及び使用法
JP2003102491A (ja) 生物発光タンパク質
US8552151B2 (en) Mutant blue fluorescent protein and method of using the same for fluorescence energy transfer and blue fluorescent fish
Branchini et al. Mutagenesis and structural studies reveal the basis for the activity and stability properties that distinguish the Photinus Luciferases scintillans and pyralis
US8722376B2 (en) Firefly luciferase
JP6006070B2 (ja) ホタル由来ルシフェラーゼ
JP7668575B2 (ja) 発光反応を触媒するペプチド
CN103429613B (zh) 源自卵翅锯角萤的荧光素酶
JP7356749B2 (ja) 改変型ルシフェラーゼ
TWI412589B (zh) 突變藍色螢光蛋白及其用於螢光共振能量傳遞與藍色螢光魚之方法
JP5980608B2 (ja) ホタル由来ルシフェラーゼ
Duca Characterizing the Utility of a Near Infrared Click Beetle Luciferase for BLI Applications
JP2014018191A (ja) オキナワマドボタルに由来するルシフェラーゼ
Roca Burgos Expression, Purification and Biophysical Characterization of ATP Independent Luciferase From Gaussia princeps
Verhaegen Engineering, overexpression, purification and analytical applications of recombinant bioluminescent reporters.

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180907

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180907

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190729

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20191029

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200106

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20200601

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200930

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20200930

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20201008

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20201012

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20201207

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20201214

R150 Certificate of patent or registration of utility model

Ref document number: 6811173

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250