JP6707453B2 - ペプチド、ポリペプチドまたはタンパク質中の１個または複数の翻訳後修飾（ｐｔｍ）の存在、非存在、数または位置を決定する方法 - Google Patents

ペプチド、ポリペプチドまたはタンパク質中の１個または複数の翻訳後修飾（ｐｔｍ）の存在、非存在、数または位置を決定する方法 Download PDF

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JP6707453B2
JP6707453B2 JP2016543461A JP2016543461A JP6707453B2 JP 6707453 B2 JP6707453 B2 JP 6707453B2 JP 2016543461 A JP2016543461 A JP 2016543461A JP 2016543461 A JP2016543461 A JP 2016543461A JP 6707453 B2 JP6707453 B2 JP 6707453B2
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protein
pore
polypeptide
peptide
seq
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ベイリー，ヘイガン
ロドリゲス−ラレア，デイビッド
ベク ロースン，クリスチャン
ベク ロースン，クリスチャン
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オックスフォード ユニヴァーシティ イノヴェーション リミテッド
オックスフォード ユニヴァーシティ イノヴェーション リミテッド
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/10—Post-translational modifications [PTMs] in chemical analysis of biological material acylation, e.g. acetylation, formylation, lipoylation, myristoylation, palmitoylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2016543461A 2013-09-23 2014-09-22 ペプチド、ポリペプチドまたはタンパク質中の１個または複数の翻訳後修飾（ｐｔｍ）の存在、非存在、数または位置を決定する方法 Active JP6707453B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1316849.7 2013-09-23
GBGB1316849.7A GB201316849D0 (en) 2013-09-23 2013-09-23 Method
US201361896933P 2013-10-29 2013-10-29
US61/896,933 2013-10-29
PCT/GB2014/052873 WO2015040423A1 (en) 2013-09-23 2014-09-22 Method

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JP2016532125A JP2016532125A (ja) 2016-10-13
JP2016532125A5 JP2016532125A5 (enExample) 2017-11-02
JP6707453B2 true JP6707453B2 (ja) 2020-06-10

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US (2) US10712254B2 (enExample)
EP (1) EP3049814B1 (enExample)
JP (1) JP6707453B2 (enExample)
CN (1) CN105765387B (enExample)
AU (1) AU2014322867B2 (enExample)
CA (1) CA2924752C (enExample)
GB (1) GB201316849D0 (enExample)
WO (1) WO2015040423A1 (enExample)

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GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method
GB201614455D0 (en) 2016-08-24 2016-10-05 Univ Oxford Innovation Ltd Biomarkers
JP2022500074A (ja) 2018-09-11 2022-01-04 レイクスユニフェルシテイト フローニンゲン 調節可能な孔直径を有する生物学的ナノポアと分析ツールとしてのその使用
CN109300501B (zh) * 2018-09-20 2021-02-02 国家卫生健康委科学技术研究所 蛋白质三维结构预测方法及用其构建的预测云平台
CN113677693B (zh) * 2019-04-09 2025-10-21 牛津纳米孔科技公开有限公司 孔
CN112147185B (zh) * 2019-06-29 2022-07-01 清华大学 一种控制多肽穿过纳米孔速度的方法及其应用
IL293024B1 (en) * 2019-11-19 2026-07-01 Univ Groningen Artificial insemination, methods and uses
WO2021111125A1 (en) * 2019-12-02 2021-06-10 Oxford Nanopore Technologies Limited Method of characterising a target polypeptide using a nanopore
NL2024579B1 (en) * 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
CN112480204A (zh) * 2020-04-13 2021-03-12 南京大学 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法
CN120435657A (zh) 2022-10-28 2025-08-05 格罗宁根大学 蛋白质的基于纳米孔的分析
JP2026505839A (ja) 2023-02-07 2026-02-18 オックスフォード ユニヴァーシティ イノヴェーション リミテッド ナノポアを使用してペプチド、ポリペプチドまたはタンパク質を特徴付ける方法
EP4735891A1 (en) 2023-06-30 2026-05-06 Ecole Polytechnique Federale De Lausanne (Epfl) Uses of aerolysin nanopores
GB202500666D0 (en) 2025-01-17 2025-03-05 Univ Oxford Innovation Ltd Nanopore

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US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US7744816B2 (en) * 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CA2730068A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
SG10201604316WA (en) * 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
US10139417B2 (en) * 2012-02-01 2018-11-27 Arizona Board Of Regents On Behalf Of Arizona State University Systems, apparatuses and methods for reading an amino acid sequence
CA2864035C (en) * 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
JP6312607B2 (ja) * 2012-02-16 2018-04-18 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー
NL2009191C2 (en) 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method

Also Published As

Publication number Publication date
AU2014322867B2 (en) 2020-10-08
EP3049814A1 (en) 2016-08-03
JP2016532125A (ja) 2016-10-13
CN105765387A (zh) 2016-07-13
EP3049814B1 (en) 2021-03-17
CN105765387B (zh) 2019-10-18
WO2015040423A1 (en) 2015-03-26
AU2014322867A1 (en) 2016-03-31
US10712254B2 (en) 2020-07-14
CA2924752A1 (en) 2015-03-26
US11592382B2 (en) 2023-02-28
GB201316849D0 (en) 2013-11-06
US20160209317A1 (en) 2016-07-21
US20210096053A1 (en) 2021-04-01
CA2924752C (en) 2022-11-29

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