CA2924752C - Method for determining the presence, absence, number or position(s) of one or more post-translational modifications (ptms) in a peptide, polypeptide or protein - Google Patents

Method for determining the presence, absence, number or position(s) of one or more post-translational modifications (ptms) in a peptide, polypeptide or protein Download PDF

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Publication number
CA2924752C
CA2924752C CA2924752A CA2924752A CA2924752C CA 2924752 C CA2924752 C CA 2924752C CA 2924752 A CA2924752 A CA 2924752A CA 2924752 A CA2924752 A CA 2924752A CA 2924752 C CA2924752 C CA 2924752C
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Prior art keywords
protein
pore
polypeptide
peptide
seq
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CA2924752A
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English (en)
French (fr)
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CA2924752A1 (en
Inventor
Hagan Bayley
David RODRIGUEZ-LARREA
Christian Bech ROSEN
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Oxford University Innovation Ltd
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Oxford University Innovation Ltd
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/10—Post-translational modifications [PTMs] in chemical analysis of biological material acylation, e.g. acetylation, formylation, lipoylation, myristoylation, palmitoylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2924752A 2013-09-23 2014-09-22 Method for determining the presence, absence, number or position(s) of one or more post-translational modifications (ptms) in a peptide, polypeptide or protein Active CA2924752C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1316849.7 2013-09-23
GBGB1316849.7A GB201316849D0 (en) 2013-09-23 2013-09-23 Method
US201361896933P 2013-10-29 2013-10-29
US61/896,933 2013-10-29
PCT/GB2014/052873 WO2015040423A1 (en) 2013-09-23 2014-09-22 Method

Publications (2)

Publication Number Publication Date
CA2924752A1 CA2924752A1 (en) 2015-03-26
CA2924752C true CA2924752C (en) 2022-11-29

Family

ID=49553252

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2924752A Active CA2924752C (en) 2013-09-23 2014-09-22 Method for determining the presence, absence, number or position(s) of one or more post-translational modifications (ptms) in a peptide, polypeptide or protein

Country Status (8)

Country Link
US (2) US10712254B2 (enExample)
EP (1) EP3049814B1 (enExample)
JP (1) JP6707453B2 (enExample)
CN (1) CN105765387B (enExample)
AU (1) AU2014322867B2 (enExample)
CA (1) CA2924752C (enExample)
GB (1) GB201316849D0 (enExample)
WO (1) WO2015040423A1 (enExample)

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* Cited by examiner, † Cited by third party
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GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method
GB201614455D0 (en) 2016-08-24 2016-10-05 Univ Oxford Innovation Ltd Biomarkers
JP2022500074A (ja) 2018-09-11 2022-01-04 レイクスユニフェルシテイト フローニンゲン 調節可能な孔直径を有する生物学的ナノポアと分析ツールとしてのその使用
CN109300501B (zh) * 2018-09-20 2021-02-02 国家卫生健康委科学技术研究所 蛋白质三维结构预测方法及用其构建的预测云平台
CN113677693B (zh) * 2019-04-09 2025-10-21 牛津纳米孔科技公开有限公司 孔
CN112147185B (zh) * 2019-06-29 2022-07-01 清华大学 一种控制多肽穿过纳米孔速度的方法及其应用
IL293024B1 (en) * 2019-11-19 2026-07-01 Univ Groningen Artificial insemination, methods and uses
WO2021111125A1 (en) * 2019-12-02 2021-06-10 Oxford Nanopore Technologies Limited Method of characterising a target polypeptide using a nanopore
NL2024579B1 (en) * 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
CN112480204A (zh) * 2020-04-13 2021-03-12 南京大学 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法
CN120435657A (zh) 2022-10-28 2025-08-05 格罗宁根大学 蛋白质的基于纳米孔的分析
JP2026505839A (ja) 2023-02-07 2026-02-18 オックスフォード ユニヴァーシティ イノヴェーション リミテッド ナノポアを使用してペプチド、ポリペプチドまたはタンパク質を特徴付ける方法
EP4735891A1 (en) 2023-06-30 2026-05-06 Ecole Polytechnique Federale De Lausanne (Epfl) Uses of aerolysin nanopores
GB202500666D0 (en) 2025-01-17 2025-03-05 Univ Oxford Innovation Ltd Nanopore

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US7744816B2 (en) * 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CA2730068A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
SG10201604316WA (en) * 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
US10139417B2 (en) * 2012-02-01 2018-11-27 Arizona Board Of Regents On Behalf Of Arizona State University Systems, apparatuses and methods for reading an amino acid sequence
CA2864035C (en) * 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
JP6312607B2 (ja) * 2012-02-16 2018-04-18 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー
NL2009191C2 (en) 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method

Also Published As

Publication number Publication date
AU2014322867B2 (en) 2020-10-08
EP3049814A1 (en) 2016-08-03
JP2016532125A (ja) 2016-10-13
CN105765387A (zh) 2016-07-13
EP3049814B1 (en) 2021-03-17
CN105765387B (zh) 2019-10-18
WO2015040423A1 (en) 2015-03-26
AU2014322867A1 (en) 2016-03-31
JP6707453B2 (ja) 2020-06-10
US10712254B2 (en) 2020-07-14
CA2924752A1 (en) 2015-03-26
US11592382B2 (en) 2023-02-28
GB201316849D0 (en) 2013-11-06
US20160209317A1 (en) 2016-07-21
US20210096053A1 (en) 2021-04-01

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