AU2014322867B2 - Method - Google Patents

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Publication number
AU2014322867B2
AU2014322867B2 AU2014322867A AU2014322867A AU2014322867B2 AU 2014322867 B2 AU2014322867 B2 AU 2014322867B2 AU 2014322867 A AU2014322867 A AU 2014322867A AU 2014322867 A AU2014322867 A AU 2014322867A AU 2014322867 B2 AU2014322867 B2 AU 2014322867B2
Authority
AU
Australia
Prior art keywords
protein
pore
polypeptide
peptide
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2014322867A
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English (en)
Other versions
AU2014322867A1 (en
Inventor
Hagan Bayley
David RODRIGUEZ-LARREA
Christian Bech ROSEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford University Innovation Ltd
Original Assignee
Oxford University Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford University Innovation Ltd filed Critical Oxford University Innovation Ltd
Publication of AU2014322867A1 publication Critical patent/AU2014322867A1/en
Assigned to OXFORD UNIVERSITY INNOVATION LIMITED reassignment OXFORD UNIVERSITY INNOVATION LIMITED Alteration of Name(s) of Applicant(s) under S113 Assignors: ISIS INNOVATION LIMITED
Application granted granted Critical
Publication of AU2014322867B2 publication Critical patent/AU2014322867B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/10—Post-translational modifications [PTMs] in chemical analysis of biological material acylation, e.g. acetylation, formylation, lipoylation, myristoylation, palmitoylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2014322867A 2013-09-23 2014-09-22 Method Active AU2014322867B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1316849.7 2013-09-23
GBGB1316849.7A GB201316849D0 (en) 2013-09-23 2013-09-23 Method
US201361896933P 2013-10-29 2013-10-29
US61/896,933 2013-10-29
PCT/GB2014/052873 WO2015040423A1 (en) 2013-09-23 2014-09-22 Method

Publications (2)

Publication Number Publication Date
AU2014322867A1 AU2014322867A1 (en) 2016-03-31
AU2014322867B2 true AU2014322867B2 (en) 2020-10-08

Family

ID=49553252

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2014322867A Active AU2014322867B2 (en) 2013-09-23 2014-09-22 Method

Country Status (8)

Country Link
US (2) US10712254B2 (enExample)
EP (1) EP3049814B1 (enExample)
JP (1) JP6707453B2 (enExample)
CN (1) CN105765387B (enExample)
AU (1) AU2014322867B2 (enExample)
CA (1) CA2924752C (enExample)
GB (1) GB201316849D0 (enExample)
WO (1) WO2015040423A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method
GB201614455D0 (en) 2016-08-24 2016-10-05 Univ Oxford Innovation Ltd Biomarkers
JP2022500074A (ja) 2018-09-11 2022-01-04 レイクスユニフェルシテイト フローニンゲン 調節可能な孔直径を有する生物学的ナノポアと分析ツールとしてのその使用
CN109300501B (zh) * 2018-09-20 2021-02-02 国家卫生健康委科学技术研究所 蛋白质三维结构预测方法及用其构建的预测云平台
CN113677693B (zh) * 2019-04-09 2025-10-21 牛津纳米孔科技公开有限公司 孔
CN112147185B (zh) * 2019-06-29 2022-07-01 清华大学 一种控制多肽穿过纳米孔速度的方法及其应用
IL293024B1 (en) * 2019-11-19 2026-07-01 Univ Groningen Artificial insemination, methods and uses
WO2021111125A1 (en) * 2019-12-02 2021-06-10 Oxford Nanopore Technologies Limited Method of characterising a target polypeptide using a nanopore
NL2024579B1 (en) * 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
CN112480204A (zh) * 2020-04-13 2021-03-12 南京大学 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法
CN120435657A (zh) 2022-10-28 2025-08-05 格罗宁根大学 蛋白质的基于纳米孔的分析
JP2026505839A (ja) 2023-02-07 2026-02-18 オックスフォード ユニヴァーシティ イノヴェーション リミテッド ナノポアを使用してペプチド、ポリペプチドまたはタンパク質を特徴付ける方法
EP4735891A1 (en) 2023-06-30 2026-05-06 Ecole Polytechnique Federale De Lausanne (Epfl) Uses of aerolysin nanopores
GB202500666D0 (en) 2025-01-17 2025-03-05 Univ Oxford Innovation Ltd Nanopore

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010055307A1 (en) * 2008-11-14 2010-05-20 Isis Innovation Limited Methods of enhancing translocation of charged analytes through transmembrane protein pores
WO2013123379A2 (en) * 2012-02-16 2013-08-22 The Regents Of The University Of California Nanopore sensor for enzyme-mediated protein translocation
WO2013121201A1 (en) * 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US7744816B2 (en) * 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CA2730068A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
SG10201604316WA (en) * 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
US10139417B2 (en) * 2012-02-01 2018-11-27 Arizona Board Of Regents On Behalf Of Arizona State University Systems, apparatuses and methods for reading an amino acid sequence
NL2009191C2 (en) 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010055307A1 (en) * 2008-11-14 2010-05-20 Isis Innovation Limited Methods of enhancing translocation of charged analytes through transmembrane protein pores
WO2013121201A1 (en) * 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
WO2013123379A2 (en) * 2012-02-16 2013-08-22 The Regents Of The University Of California Nanopore sensor for enzyme-mediated protein translocation

Also Published As

Publication number Publication date
EP3049814A1 (en) 2016-08-03
JP2016532125A (ja) 2016-10-13
CN105765387A (zh) 2016-07-13
EP3049814B1 (en) 2021-03-17
CN105765387B (zh) 2019-10-18
WO2015040423A1 (en) 2015-03-26
AU2014322867A1 (en) 2016-03-31
JP6707453B2 (ja) 2020-06-10
US10712254B2 (en) 2020-07-14
CA2924752A1 (en) 2015-03-26
US11592382B2 (en) 2023-02-28
GB201316849D0 (en) 2013-11-06
US20160209317A1 (en) 2016-07-21
US20210096053A1 (en) 2021-04-01
CA2924752C (en) 2022-11-29

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