JP6706210B2 - 脳および脊髄に標的化遺伝子移入するためのウイルスベクター - Google Patents
脳および脊髄に標的化遺伝子移入するためのウイルスベクター Download PDFInfo
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Description
(a)配列番号10のアミノ酸配列;
(b)配列番号10のアミノ酸配列と少なくとも80%、好ましくは90、95、または99%同一であり、およびさらに(i)配列番号1のアミノ酸配列を含むか、または(ii)1つのアミノ酸の改変により配列番号1のアミノ酸配列とは異なるアミノ酸配列を含む、アミノ酸配列;
(c)(a)または(b)において定義されたアミノ酸配列の1つの断片。
ington:The Science and Practice of Pharmacy,”Lippincott Williams & Wilkins;21st
edition(2005)に記述されている。
組織特異的AAV2カプシドを選択するために、ランダムペプチドライブラリを調製して5ラウンドで選択した。個々に存在するクローン1×108個の理論的多様性を有するランダムX7−AAVペプチドライブラリを、以前に記述された二段階プロトコール[26〜27]を使用して調製した。VP1のアミノ酸R588位に対応するAAVゲノムの3967位のヌクレオチドで7個のランダム化アミノ酸をコードする縮重オリゴヌクレオチドを最初に産生した。オリゴヌクレオチドは、配列:5’−CAGTCGGCCAGA
GAGGC(NNK)7GCCCAGGCGGCTGACGAG−3’(配列番号12)
を有した。シーケナーゼ(Amersham,Freiburg,Germany)、および配列5’−CTCGTCAGCCGCCTGG−3’(配列番号13)を有するプラ
イマーを使用して第二の鎖を産生した。二本鎖インサートをBglIによって切断し、QIAquickヌクレオチド除去キット(Qiagen,Hilden,Germany)によって精製し、SfiIによって消化済のライブラリプラスミドpMT187−0−3にライゲートした[26]。プラスミドライブラリの多様性は、150mg/mlアンピシリンを含有する寒天上で、形質転換されたエレクトロコンピテントDH5α細菌の代表的なアリコートから生育させたクローン数によって決定した。ライブラリプラスミドを採取して、Qiagenのプラスミド調製キットを使用して精製した。細胞培養皿(15cm)10枚において293T細胞2×108個に、プラスミドpVP3cm(末端逆位配列を有しない改変コドン使用を有する野生型cap遺伝子を含有する)[27]、ライブラリプラスミド、およびpXX6ヘルパープラスミド[28]を、プラスミド間の比率1:1;2でトランスフェクトさせることによって、AAVライブラリゲノムを、キメラ野生型およびライブラリAAVカプシド(AAV移行シャトル)にパッケージングした。得られたAAVライブラリ移行シャトルを使用して、細胞培養皿(15cm)において293T細胞2×108個にMOI 0.5複製単位/細胞で感染させた。細胞に、Ad5(the Laboratoire de Therapie Genique,France)を、MOI 5プラーク形成単位(pfu/細胞)で重複感染させた。最終的なAAVディスプレイライブラリを48時間後に上清から採取した。上清をVivaSpinカラム(Viva Science,Hannover,Germany)を使用して濃縮し、以前に記述されたように[29]イオジキサノール密度勾配超遠心分離によって精製し、cap特異的プライマー5’−GCAGTATGGTTCTGTATCTACC
AACC−3’(配列番号14)および5’−GCCTGGAAGAACGCCTTGT
GTG−3’(配列番号15)を使用してLightCyclerシステム(Roche
Diagnostics,Mannheim,Germany)によるリアルタイムPCRによって力価を測定した。
CAAGCCACAAGGACGATG−3’(配列番号16)および5’−CGTGG
AGTACTGTGTGATGAAG−3’(配列番号17)を使用し、ならびに第二の
PCRに関してプライマー5’−GGTTCTCATCTTTGGGAAGCAAG−3
’(配列番号18)および5−TGATGAGAATCTGTGGAGGAG−3’(配
列番号19)を使用するネステッドPCRによって増幅した。PCR増幅されたオリゴヌクレオチドを使用して、さらに3ラウンドの選択のために二次ライブラリを調製した。二次ライブラリは、一次ライブラリ(上記を参照)と同様に生成したが、移行シャトルを産生する追加の工程を行わなかった。二次プラスミドライブラリを使用して、細胞培養皿(15cm)においてトランスフェクション試薬Polyfect(Qiagen)を使用して、ライブラリプラスミド25個/細胞の比率で293T細胞2×108個にトランスフェクトさせた。各ラウンドの選択後、いくつかのクローンをシークエンシングした。適用した選択法を図1に示す。
実施例1において濃縮されたクローンを組み換え型AAVベクターとして産生し、その形質導入プロファイルを試験した。組み換え型AAVベクターは、HEK293T細胞のトリプルトランスフェクションによって産生した。細胞を37℃、5%CO2で、1%ペニシリン/ストレプトマイシンおよび10%ウシ胎仔血清を添加したダルベッコ改変イーグル培地(Invitrogen,Carlsbad,USA)においてインキュベートした。プラスミドDNAを、トランスフェクト剤Polyfect(Qiagen,Hilden,Germany)によって293T細胞にトランスフェクトさせた。トランスフェクションの4日後、細胞を採取して溶解し、ベクターを、以前に記述された[29]イオジキサノール密度勾配超遠心分離によって精製した。トランスフェクションに関して、関心対象のAAVカプシドをコードするプラスミドと同様に、ルシフェラーゼ遺伝子pUF2−CMV−luc[27]またはGFP遺伝子pTR−CMV−GFP[30]をコードするpXX6を、アデノウイルスヘルパープラスミド[28]として使用した。AAVライブラリから先に選択されているAAVカプシド変異体および野生型対照をコードするプラスミドは、改変pXX2−187[31]またはpXX2[28]であった。インサートを、記述のようにライブラリインサートへと処理した(上記を参照されたい)。組み換え型ベクターを定量するために、ゲノムの力価を、以前に記述されたように[32]、CMV特異的プライマー5’−GGCGGAGTTGTTACGACAT−3’(配列番号20)、および5’−GGGACTTTCCCTACTTGGCA−3’(配列番号21)を使用するリアルタイムPCRによって、LightCyclerシステムによって決定した。
インビボで濃縮されたペプチドの指向性を調べることができるように、ペプチドを、ルシフェラーゼレポーター遺伝子を含む組み換え型ベクターのカプシドに導入した。変異したカプシドを有するベクターを、対照ベクターと共にマウスに注射した。AAVベクターを、ベクターゲノム(vg)5×1010個/マウス(注射したAAVクローンあたり、動物n=3)の用量で静脈内投与した。14日目に、動物をイソフルランで麻酔した。ルシフェリン基質(150mg/kg、Xenogen)200μl/マウスの腹腔内注射後のルシフェラーゼ発現を、Xenogen IVIS200イメージングシステム(Caliper Lifescience,Hopkinton,USA)を使用して、Living Image4.0(Caliper)ソフトウェアによって分析した。異なる位置(腹側、背側、外側)でのトランスジーン発現の代表的なインビボ生物発光画像を、相対発光量(光子/秒/cm2)での発光が最高強度に達したときに撮影した。
静脈内に注射したNRGTEWDベクターのトランスジーンの脳特異的発現が特異的ホーミングに基づくものかを調べるために、最初に、5×1010gp/マウスの静脈内投与後14日目にベクターの分布を調べた。ベクターゲノムの定量は、リアルタイムPCRによって行った。最初に、組織ホモジナイザー(Precellys 24,Peqlab,Erlangen,Germany)およびDNeasy組織キット(Qiagen,Hilden,Germany)を使用して、製造元の説明書に従って、5×1010vg/マウスの静脈内投与後の様々な時点で、関係する臓器から総DNAを抽出した。DNAを、分光光度計(NanoDrop,ND−2000C、Peqlab)を使用して定量した。組織におけるAAVベクターDNAの分析は、上記のCMV特異的プライマーを使用して、鋳型40ngを使用する定量的リアルタイムPCRによって行い、総DNAに関して標準化した。
免疫組織化学を使用して、ペプチドNRGTEWDおよび/または対照としての野生型AAVカプシドを有するrAAV−GFPベクターの静脈内投与後14日に、脳ならびに対照臓器の細胞レベルでトランスジーンの発現を可視化した。動物の脳を4%(w/v)パラホルムアルデヒドで固定した。組織をパラフィンに包埋した。厚さ2μmの切片のロウを除去して、再度水和させ、免疫組織化学のために使用した。免疫組織化学技法は、GFP(A−11122,Invitrogen)またはCD31(AB28364、Abcam,Cambridge,USA)のポリクローナル抗体を使用して行った。内因性のペルオキシダーゼの活性を、1%H2O2のメタノール溶液によって30分間不活化した。CD31による染色の前に、切片をクエン酸緩衝液(pH6.0)中、100℃で20分間加熱した。PBSで洗浄後、切片をPBS、10%ヤギ血清(Vector Lab,Burlingame,USA)および2%粉乳(Roth)と共に30分間インキュベートした。一次抗体を37℃で1時間結合させた。PBSで洗浄後、切片を二次ビオチニル化ヤギ抗ウサギ抗体(Vector Lab)と共に30分間インキュベートした。結合した抗体を、VECTASTAIN−Elite ABCキット(Vector Lab)および3,3’−ジアミノベンジデン(3,3−diaminobenzidene)(DAB,Sigma−Aldrich,St.Louis,USA)を使用して可視化した。選択した切片をヘマラムで対比染色した。
Sf9昆虫細胞[33〜35]における組み換え型AAVベクターの産生に関して、cap遺伝子にペプチド挿入をコードする(上記を参照されたい)オリゴヌクレオチドインサートを有する改変AAV2ゲノムを、ドナープラスミドpFASTBAC Dual(Life Technologies,Darmstadt,Germany)にクローニングした。加えて、人工のイントロンを、polhプロモーターを含むドナープラスミドに挿入し、それによってプラスミドpFBD−Repin/Capin[35]を得た。ドナープラスミドpFB−CAG−eGFPを確立するために、CAGプロモーターおよびeGFP遺伝子を、SV40ポリアデニル化シグナルおよびAAV2 ITRと共にプラスミドpFASTBAC1(Life Technologies)にクローニングした。ドナープラスミドを、DH10Bac大腸菌(E.coli)細胞を形質転換するために使用して、次にDH10Bac大腸菌細胞を、組み換え型AAVゲノムまたはeGFPトランスジーンカセットをそれぞれ含む組み換え型バクミドを単離するために使用した。バクミド(9μg)を、Fectoflyトランスフェクション試薬(Polyplus Transfection/VWR International GmbH,Darmstadt,Germany)を使用して6ウェルフォーマットでSf9細胞1×106個のトランスフェクションに使用した。トランスフェクトしたSf9昆虫細胞を、1%ゲンタマイシン(Lonza)を含む昆虫X−Press培地(Lonza,Cologne,Germany)において27℃3日間インキュベーション後、細胞培養上清に存在する組み換え型バキュロウイルス500μlを、T175細胞培養フラスコにおいて1%ゲンタマイシンを含む昆虫X−Press培地(Lonza)中で、新たなSf9細胞2.5×107個を27℃でさらに3日間増幅するために使用した。このようにして増幅されたバキュロウイルスを、組み換え型AAVベクターを産生するために新たなSf9細胞を感染させるために使用した。この目的に関して、AAVゲノムが挿入された組み換え型バキュロウイルスおよびeGFPトランスジーンカセットが挿入された組み換え型バキュロウイルスを混合して、1Lアーレンマイヤーフラスコにおいて1%ゲンタマイシンを含む昆虫X−Press培地400ml中で、昆虫細胞6×108個を感染させるために共に使用した。次に、細胞を撹拌(110rpm)しながら27℃でインキュベートした。感染後4日目に、細胞を採取して溶解し、AAVベクターを、前に記述された[29]イオジキサノール密度勾配超遠心分離によって精製した。組み換え型ベクターの定量に関して、ゲノム力価を、配列番号20および配列番号21のCMV特異的プライマーを使用して、前に記述されたように[32]LightCyclerシステムで定量的リアルタイムPCRによって決定した。
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Claims (25)
- 配列番号1のアミノ酸配列を含むことを特徴とする、ウイルスベクターのカプシドタンパク質。
- アデノ随伴ウイルス(AAV)のカプシドタンパク質である、請求項1に記載のカプシドタンパク質。
- 血清型2、4、6、8および9からなる群から選択される血清型のAAVのカプシドタンパク質である、請求項2に記載のカプシドタンパク質。
- 血清型2のAAVのカプシドタンパク質である、請求項3に記載のカプシドタンパク質。
- 血清型2のAAVのVP1タンパク質である、請求項4に記載のカプシドタンパク質。
- 前記ペプチドがカプシドの550〜600位のアミノ酸の領域に存在する、請求項1〜5のいずれか1項に記載のカプシドタンパク質。
- 配列番号10のアミノ酸配列を含む、請求項1〜6のいずれか1項に記載のカプシドタンパク質。
- 請求項1〜7のいずれか1項に記載のカプシドタンパク質を含むウイルスカプシド。
- 請求項1〜7のいずれか1項に記載のカプシドタンパク質をコードする核酸。
- 前記カプシドタンパク質が配列番号10のアミノ酸配列を含む、請求項9に記載の核酸。
- 請求項9又は10に記載の核酸を含むプラスミド。
- カプシドと、カプシドの中にパッケージングされたトランスジーンとを含む、組み換え型ウイルスベクターであって、該カプシドが、請求項1〜7のいずれか1項に記載のカプシドタンパク質を含む少なくとも1つのカプシドタンパク質を含む組み換え型ウイルスベクター。
- 前記カプシドタンパク質が配列番号10のアミノ酸配列を含む、請求項12に記載の組み換えウイルスベクター。
- 組み換え型AAVベクターである、請求項12または13に記載の組み換え型ウイルスベクター。
- 血清型2、4、6、8および9からなる群より選択される血清型のAAVベクターである、請求項14記載の組み換え型ウイルスベクター。
- 血清型2のAAVベクターである、請求項15記載の組み換え型ウイルスベクター。
- 前記トランスジーンが以下のタンパク質:クローディンまたはオクルディンなどの膜または密着結合タンパク質、ノイラミニダーゼ1などのノイラミニダーゼ、グルクロニダーゼ、CCL2−7NDなどのケモカインアンタゴニスト、グリア細胞の神経栄養因子(GDNF)、ネプリライシン、コレステロール24ヒドロキシラーゼ、芳香族L−アミノ酸デカルボキシラーゼ、チロシンヒドロキシラーゼ、GTPシクロヒドロラーゼIおよび生存運動ニューロン(SMN)タンパク質、の1つをコードする、請求項12〜16のいずれか1項に記載の組み換え型ウイルスベクター。
- 前記トランスジーンがノイラミニダーゼをコードする、請求項17に記載の組み換え型ウイルスベクター。
- 前記トランスジーンがssDNAまたはdsDNAの形状である、請求項12〜18のいずれか1項に記載の組み換え型ウイルスベクター。
- 対象における脳および/または脊髄の機能障害または疾患を処置する方法に使用するための、請求項12〜18のいずれか1項に記載の組み換え型ウイルスベクター。
- 脳および/または脊髄の前記疾患が、副腎白質ジストロフィー、カナンバン(Cananvan)病、クラッベ病、異染性白質ジストロフィー、ペリツェウス・メルツバッハー病、およびアレキサンダー病などの、遺伝的に引き起こされた白質ジストロフィー;筋委縮性側索硬化症、アルツハイマー病、パーキンソン病、ハンチントン病、およびピック病などの神経変性疾患;多発性硬化症およびギラン・バレー症候群などの中枢神経系の慢性炎症疾患;ならびにセロイド・リポフスチン症およびファブリー病などのライソゾーム蓄積病からなる群より選択される、請求項20に記載の方法に使用するための組み換え型ウイルスベクター。
- 前記対象が哺乳動物、好ましくはヒトである、方法における使用のための請求項20または21に記載の組み換え型ウイルスベクター。
- 静脈内投与のために処方される、請求項20〜22のいずれか1項に記載の方法に使用するための組み換え型ウイルスベクター。
- 請求項1〜7のいずれか1項に記載のカプシドタンパク質、請求項8に記載のウイルスカプシド、請求項9または10に記載の核酸、請求項11に記載のプラスミド、または請求項12〜18のいずれか1項に記載の組み換え型ウイルスベクター、を含む細胞。
- 請求項1〜7のいずれか1項に記載のカプシドタンパク質、請求項8に記載のウイルスカプシド、請求項9または10に記載の核酸、請求項11に記載のプラスミド、または請求項12〜18のいずれか1項に記載の組み換え型ウイルスベクター、を含む医薬組成物。
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DE102014207498.3A DE102014207498A1 (de) | 2014-04-17 | 2014-04-17 | Viraler Vektor für den zielgerichteten Gentransfer in Gehirn und Rückenmark |
DE102014207498.3 | 2014-04-17 | ||
PCT/EP2015/058123 WO2015158749A2 (de) | 2014-04-17 | 2015-04-15 | Viraler vektor für den zielgerichteten gentransfer in gehirn und rückenmark |
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DE102013215817A1 (de) | 2013-08-09 | 2015-02-12 | Universitätsklinikum Hamburg-Eppendorf | Neue peptide mit spezifität für die lunge |
US10087224B2 (en) * | 2013-11-01 | 2018-10-02 | Cornell University | Gene therapy for Alzheimer's and other neurodegenerative diseases and conditions |
DE102014207498A1 (de) | 2014-04-17 | 2015-10-22 | Universitätsklinikum Hamburg-Eppendorf | Viraler Vektor für den zielgerichteten Gentransfer in Gehirn und Rückenmark |
EP3500278B1 (en) | 2016-08-19 | 2024-05-22 | University of Florida Research Foundation, Incorporated | Compositions for treating conditions using recombinant self-complementary adeno-associated virus |
CA3046347A1 (en) | 2016-12-07 | 2018-06-14 | University Of Florida Research Foundation, Incorporated | Il-1ra cdnas |
CA3059891A1 (en) | 2017-04-14 | 2018-10-18 | National Taiwan University | Gene therapy for aadc deficiency |
PL3740222T3 (pl) * | 2018-01-17 | 2023-12-04 | Meiragtx Uk Ii Limited | Zmodyfikowane białko kapsydu raav do terapii genowej |
US20210015898A1 (en) * | 2018-04-09 | 2021-01-21 | Allen Institute | Rescuing voltage-gated sodium channel function in inhibitory neurons |
US20220162637A1 (en) * | 2019-04-24 | 2022-05-26 | Takara Bio Inc. | Aav mutant having brain-targeting property |
KR20230129245A (ko) | 2020-12-23 | 2023-09-07 | 베링거 인겔하임 인터내셔날 게엠베하 | 심장 조직 세포에 특이성을 갖는 바이러스 캡시드 단백질 |
EP4359547A1 (en) * | 2021-06-22 | 2024-05-01 | Pfizer Inc. | Production of adeno-associated virus vector in insect cells |
WO2023154763A2 (en) * | 2022-02-08 | 2023-08-17 | Spacecraft Seven, Llc | Adeno-associated viral vector for glut1 expression and uses thereof |
WO2024079317A1 (en) * | 2022-10-14 | 2024-04-18 | Institut National de la Santé et de la Recherche Médicale | Methods and pharmaceutical composition for the treatment of alpha-synucleinopathies |
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US20040181830A1 (en) * | 2001-05-07 | 2004-09-16 | Kovalic David K. | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
US20030148264A1 (en) * | 2001-07-06 | 2003-08-07 | Genentech, Inc. | Phage displayed PDZ domain ligands |
US7314974B2 (en) * | 2002-02-21 | 2008-01-01 | Monsanto Technology, Llc | Expression of microbial proteins in plants for production of plants with improved properties |
WO2004083441A2 (en) | 2003-03-19 | 2004-09-30 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Random peptide library displayed on aav vectors |
US7427396B2 (en) | 2004-06-03 | 2008-09-23 | Genzyme Corporation | AAV vectors for gene delivery to the lung |
SI2029742T1 (sl) * | 2006-06-07 | 2016-11-30 | Genzyme Corporation | Genska terapija amiotrofične lateralne skleroze in drugih motenj hrbtenjače |
WO2009012176A2 (en) * | 2007-07-14 | 2009-01-22 | The University Of Iowa Research Foundation | Methods and compositions for treating brain diseases |
WO2010127097A1 (en) | 2009-04-30 | 2010-11-04 | The Trustees Of The University Of Pennsylvania | Compositions for targeting conducting airway cells comprising adeno-associated virus constructs |
WO2012057363A1 (ja) * | 2010-10-27 | 2012-05-03 | 学校法人自治医科大学 | 神経系細胞への遺伝子導入のためのアデノ随伴ウイルスビリオン |
JP6426001B2 (ja) * | 2011-11-17 | 2018-11-21 | グリア エスピー ゼット.オー.オー. | 神経膠腫を治療するための組成物および方法 |
DE102014207498A1 (de) | 2014-04-17 | 2015-10-22 | Universitätsklinikum Hamburg-Eppendorf | Viraler Vektor für den zielgerichteten Gentransfer in Gehirn und Rückenmark |
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WO2015158749A2 (de) | 2015-10-22 |
US10287318B2 (en) | 2019-05-14 |
WO2015158749A3 (de) | 2016-02-04 |
EP3132043B1 (de) | 2019-03-06 |
EP3722437A1 (de) | 2020-10-14 |
EP3533877A3 (de) | 2019-10-23 |
EP3533877A2 (de) | 2019-09-04 |
US20170029464A1 (en) | 2017-02-02 |
US20210147480A1 (en) | 2021-05-20 |
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