JP6603663B2 - マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法 - Google Patents

マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法 Download PDF

Info

Publication number
JP6603663B2
JP6603663B2 JP2016540965A JP2016540965A JP6603663B2 JP 6603663 B2 JP6603663 B2 JP 6603663B2 JP 2016540965 A JP2016540965 A JP 2016540965A JP 2016540965 A JP2016540965 A JP 2016540965A JP 6603663 B2 JP6603663 B2 JP 6603663B2
Authority
JP
Japan
Prior art keywords
cells
enclosure
cell
nucleic acid
capture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2016540965A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017504315A (ja
JP2017504315A5 (cg-RX-API-DMAC7.html
Inventor
ティー． チャプマン，ケヴィン
ディー． ホッブズ，エリック
ダブリュ． ショート，スティーヴン
ピー． ホワイト，マーク
マレオ，ダニエレ
Original Assignee
バークレー ライツ，インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by バークレー ライツ，インコーポレイテッド filed Critical バークレー ライツ，インコーポレイテッド
Publication of JP2017504315A publication Critical patent/JP2017504315A/ja
Publication of JP2017504315A5 publication Critical patent/JP2017504315A5/ja
Priority to JP2019188011A priority Critical patent/JP6954972B2/ja
Application granted granted Critical
Publication of JP6603663B2 publication Critical patent/JP6603663B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Electrochemistry (AREA)
  • Fluid Mechanics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Computer Hardware Design (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2016540965A 2013-12-18 2014-12-18 マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法 Active JP6603663B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2019188011A JP6954972B2 (ja) 2013-12-18 2019-10-11 マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/133,361 US20150166326A1 (en) 2013-12-18 2013-12-18 Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device
US14/133,361 2013-12-18
PCT/US2014/071323 WO2015095623A1 (en) 2013-12-18 2014-12-18 Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019188011A Division JP6954972B2 (ja) 2013-12-18 2019-10-11 マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法

Publications (3)

Publication Number Publication Date
JP2017504315A JP2017504315A (ja) 2017-02-09
JP2017504315A5 JP2017504315A5 (cg-RX-API-DMAC7.html) 2018-02-01
JP6603663B2 true JP6603663B2 (ja) 2019-11-06

Family

ID=53367562

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2016540965A Active JP6603663B2 (ja) 2013-12-18 2014-12-18 マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法
JP2019188011A Active JP6954972B2 (ja) 2013-12-18 2019-10-11 マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2019188011A Active JP6954972B2 (ja) 2013-12-18 2019-10-11 マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法

Country Status (7)

Country Link
US (1) US20150166326A1 (cg-RX-API-DMAC7.html)
EP (1) EP3083980B1 (cg-RX-API-DMAC7.html)
JP (2) JP6603663B2 (cg-RX-API-DMAC7.html)
KR (2) KR102448505B1 (cg-RX-API-DMAC7.html)
DK (1) DK3083980T3 (cg-RX-API-DMAC7.html)
SG (2) SG10201902353UA (cg-RX-API-DMAC7.html)
WO (1) WO2015095623A1 (cg-RX-API-DMAC7.html)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9403172B2 (en) * 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
US20150166326A1 (en) * 2013-12-18 2015-06-18 Berkeley Lights, Inc. Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
IL299366A (en) 2015-11-23 2023-02-01 Berkeley Lights Inc In situ-generated microfluidic isolation structures, kits and methods of use thereof
TWI808934B (zh) 2015-12-31 2023-07-21 美商伯克利之光生命科技公司 經工程化以表現促發炎多肽之腫瘤浸潤細胞
WO2017160991A1 (en) 2016-03-16 2017-09-21 Lavieu Gregory G Methods, systems and devices for selection and generation of genome edited clones
CN109311930B (zh) * 2016-03-31 2023-02-24 伯克利之光生命科技公司 核酸稳定试剂、试剂盒及其使用方法
CN110248733B (zh) * 2016-12-01 2022-01-11 伯克利之光生命科技公司 用于对微物体成像的设备、系统和方法
CA3045333A1 (en) * 2016-12-01 2018-06-07 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
EP3551745A4 (en) 2016-12-12 2020-07-15 Xcella Biosciences, Inc. SCREENING METHODS AND SYSTEMS USING MICROCAPILLARY NETWORKS
CA3048645A1 (en) 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
US11156626B2 (en) 2016-12-30 2021-10-26 xCella Biosciences, Inc. Multi-stage sample recovery system
WO2018207454A1 (ja) * 2017-05-12 2018-11-15 株式会社ニコン 対象物操作装置、及び対象物操作方法
WO2018226900A2 (en) 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
KR20210018219A (ko) 2018-06-06 2021-02-17 지머젠 인코포레이티드 발효 및 생산 중 곰팡이 형태를 제어하기 위한 신호 전달에 포함된 유전자의 조작
KR20210078510A (ko) 2018-10-11 2021-06-28 버클리 라잇츠, 인크. 최적화된 단백질 생성의 확인을 위한 시스템 및 방법, 및 이를 위한 키트
EP3883692A4 (en) * 2018-11-19 2022-08-31 Berkeley Lights, Inc. MICROFLUID DEVICE WITH PROGRAMMABLE SWITCHING ELEMENTS
US12318775B2 (en) 2018-12-06 2025-06-03 xCella Biosciences, Inc. Lateral loading of microcapillary arrays
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6685812B2 (en) * 2001-01-09 2004-02-03 The Regents Of The University Of California Movement of particles using sequentially activated dielectrophoretic particle trapping
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
AU2003299541A1 (en) * 2002-10-02 2004-05-25 California Institute Of Technology Microfluidic nucleic acid analysis
WO2004065618A2 (en) * 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
US20050142565A1 (en) * 2003-12-30 2005-06-30 Agency For Science, Technology And Research Nucleic acid purification chip
JP2007537729A (ja) * 2004-04-12 2007-12-27 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 微粒子及び細胞の操作用の光電子ピンセット
EP3029135B1 (en) * 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
WO2007102839A2 (en) * 2005-10-27 2007-09-13 Applera Corporation Optoelectronic separation of biomolecules
CN101415813B (zh) * 2006-02-03 2013-04-10 微芯片生物工艺学股份有限公司 微流体装置
US9040288B2 (en) * 2006-03-24 2015-05-26 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US7439014B2 (en) * 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
KR101228308B1 (ko) * 2007-05-23 2013-01-31 삼성전자주식회사 미세유동 칩을 이용한 디스크형 미세유동장치 및 생체물질마이크로어레이 칩을 이용한 디스크형 미세유동장치
ATE554859T1 (de) * 2007-05-24 2012-05-15 Univ California Integrierte fluidische vorrichtungen mit magnetischer sortierung
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
EP2606154B1 (en) * 2010-08-20 2019-09-25 Integenx Inc. Integrated analysis system
US9506845B2 (en) * 2012-02-29 2016-11-29 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
US20150166326A1 (en) * 2013-12-18 2015-06-18 Berkeley Lights, Inc. Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device

Also Published As

Publication number Publication date
EP3083980A4 (en) 2017-08-30
SG11201604907SA (en) 2016-07-28
DK3083980T3 (da) 2021-12-13
SG10201902353UA (en) 2019-04-29
JP2017504315A (ja) 2017-02-09
KR20160098341A (ko) 2016-08-18
KR102220001B1 (ko) 2021-02-25
KR102448505B1 (ko) 2022-09-27
JP6954972B2 (ja) 2021-10-27
WO2015095623A1 (en) 2015-06-25
JP2020022479A (ja) 2020-02-13
US20150166326A1 (en) 2015-06-18
EP3083980B1 (en) 2021-10-13
KR20210022146A (ko) 2021-03-02
EP3083980A1 (en) 2016-10-26

Similar Documents

Publication Publication Date Title
JP6603663B2 (ja) マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法
US11318479B2 (en) Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
Kovac et al. Intuitive, image-based cell sorting using optofluidic cell sorting
TWI758265B (zh) 原位產生之微流體分析結構，相關套組，及其使用方法
Voldman et al. A microfabrication-based dynamic array cytometer
US10578630B2 (en) Automated identification of assay areas in a microfluidic device and detection of assay positive areas based on rate of change of image light intensity
KR20190069456A (ko) 미세유체 장치에서 인 시츄 식별을 위한 dna 바코드 조성물 및 방법
CN109311930B (zh) 核酸稳定试剂、试剂盒及其使用方法
US20160257918A1 (en) Generation and Selection of Embryos in Vitro
CN106255888A (zh) 在同一微流体装置的不同部分中的dep力控制和电润湿控制
US20150360236A1 (en) Methods and devices for sorting cells and other biological particulates
JP2017504315A5 (cg-RX-API-DMAC7.html)
CN109142717B (zh) 用于测定生物活性的微流体装置
CN105658781A (zh) 具有隔离围栏的微流体装置及用它测试生物微目标的方法
US20220379320A1 (en) Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
CN107427315A (zh) 体外胚胎的生殖和选择
Berendsen The entrapment and characterization of single spermatozoa in a microfluidic system
HK1225407B (zh) 具有隔离围栏的微流体装置及用它测试生物微目标的方法
HK40016859B (en) Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171218

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20171218

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20180910

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20180914

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20181204

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20190304

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190409

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20190815

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20190912

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20191011

R150 Certificate of patent or registration of utility model

Ref document number: 6603663

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250