SG10201902353UA - Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device - Google Patents

Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device

Info

Publication number
SG10201902353UA
SG10201902353UA SG10201902353UA SG10201902353UA SG10201902353UA SG 10201902353U A SG10201902353U A SG 10201902353UA SG 10201902353U A SG10201902353U A SG 10201902353UA SG 10201902353U A SG10201902353U A SG 10201902353UA SG 10201902353U A SG10201902353U A SG 10201902353UA
Authority
SG
Singapore
Prior art keywords
micro
nucleic acid
specific nucleic
fluidic device
biological cells
Prior art date
Application number
SG10201902353UA
Inventor
Kevin T Chapman
Eric D Hobbs
Steven W Short
Mark P White
Daniele Malleo
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG10201902353UA publication Critical patent/SG10201902353UA/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Abstract

METHODS AND COMPOSITIONS FOR TREATMENT OF ATTENTION DEFICIT DISORDER Therapeutic compositions and methods for treatment of attention deficit disorder (ADD) or attention deficit hyperactivity disorder (ADHD) include dosage forms that deliver a therapeutic amount of active drug in a delayed and controlled release formulation. The dosage form can be administered at night and drug release is delayed for from 3 to 8 hours, followed by an ascending release rate. FIGURE 3
SG10201902353UA 2013-12-18 2014-12-18 Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device SG10201902353UA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US14/133,361 US20150166326A1 (en) 2013-12-18 2013-12-18 Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device

Publications (1)

Publication Number Publication Date
SG10201902353UA true SG10201902353UA (en) 2019-04-29

Family

ID=53367562

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201604907SA SG11201604907SA (en) 2013-12-18 2014-12-18 Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
SG10201902353UA SG10201902353UA (en) 2013-12-18 2014-12-18 Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201604907SA SG11201604907SA (en) 2013-12-18 2014-12-18 Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device

Country Status (7)

Country Link
US (1) US20150166326A1 (en)
EP (1) EP3083980B1 (en)
JP (2) JP6603663B2 (en)
KR (2) KR102220001B1 (en)
DK (1) DK3083980T3 (en)
SG (2) SG11201604907SA (en)
WO (1) WO2015095623A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9403172B2 (en) * 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
US20150166326A1 (en) * 2013-12-18 2015-06-18 Berkeley Lights, Inc. Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
IL299366A (en) 2015-11-23 2023-02-01 Berkeley Lights Inc In situ-generated microfluidic isolation structures, kits and methods of use thereof
TWI808934B (en) 2015-12-31 2023-07-21 美商伯克利之光生命科技公司 Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
CN109922885B (en) 2016-03-16 2022-05-10 伯克利之光生命科技公司 Methods, systems and apparatus for selection and generation of genome editing clones
DK3436469T3 (en) * 2016-03-31 2022-03-21 Berkeley Lights Inc NUCLEIC ACID STABILIZATION REAGENTS, KITS AND METHODS OF USING IT
AU2017368268A1 (en) * 2016-12-01 2019-06-20 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
CN114414510A (en) 2016-12-01 2022-04-29 伯克利之光生命科技公司 Apparatus, system and method for imaging micro-objects
CA3046827A1 (en) 2016-12-12 2018-06-21 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
CN110546495B (en) 2016-12-30 2022-11-01 加利福尼亚州立大学董事会 Methods for selection and passage of genome editing T cells
WO2018207454A1 (en) * 2017-05-12 2018-11-15 株式会社ニコン Object manipulation device and object manipulation method
KR20200026878A (en) 2017-06-06 2020-03-11 지머젠 인코포레이티드 HTP Genome Engineering Platform to Improve Fungal Strains
CN112166180A (en) 2018-06-06 2021-01-01 齐默尔根公司 Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
SG11202104544WA (en) * 2018-11-19 2021-06-29 Berkeley Lights Inc Microfluidic device with programmable switching elements
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6685812B2 (en) * 2001-01-09 2004-02-03 The Regents Of The University Of California Movement of particles using sequentially activated dielectrophoretic particle trapping
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
WO2004040001A2 (en) * 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
WO2004065618A2 (en) * 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
US20050142565A1 (en) 2003-12-30 2005-06-30 Agency For Science, Technology And Research Nucleic acid purification chip
EP1735428A4 (en) * 2004-04-12 2010-11-10 Univ California Optoelectronic tweezers for microparticle and cell manipulation
EP3029135B1 (en) * 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
WO2007102839A2 (en) * 2005-10-27 2007-09-13 Applera Corporation Optoelectronic separation of biomolecules
WO2008030631A2 (en) * 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
DK3088083T3 (en) * 2006-03-24 2018-11-26 Handylab Inc Method of carrying out PCR down a multi-track cartridge
US7439014B2 (en) * 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
KR101228308B1 (en) * 2007-05-23 2013-01-31 삼성전자주식회사 Disk type microfluidic device using microfluidic chip and disk type microfluidic device using biomolecule microarray chip
US20080302732A1 (en) * 2007-05-24 2008-12-11 Hyongsok Soh Integrated fluidics devices with magnetic sorting
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
EP2606154B1 (en) * 2010-08-20 2019-09-25 Integenx Inc. Integrated analysis system
SG10201608159XA (en) * 2012-02-29 2016-11-29 Fluidigm Corp Methods, systems, and devices for multiple single-cell capturing and processing using microfluidics
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
US20150166326A1 (en) * 2013-12-18 2015-06-18 Berkeley Lights, Inc. Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device

Also Published As

Publication number Publication date
WO2015095623A1 (en) 2015-06-25
DK3083980T3 (en) 2021-12-13
KR20210022146A (en) 2021-03-02
EP3083980B1 (en) 2021-10-13
EP3083980A1 (en) 2016-10-26
KR20160098341A (en) 2016-08-18
US20150166326A1 (en) 2015-06-18
SG11201604907SA (en) 2016-07-28
EP3083980A4 (en) 2017-08-30
JP2017504315A (en) 2017-02-09
JP6954972B2 (en) 2021-10-27
KR102220001B1 (en) 2021-02-25
KR102448505B1 (en) 2022-09-27
JP2020022479A (en) 2020-02-13
JP6603663B2 (en) 2019-11-06

Similar Documents

Publication Publication Date Title
SG10201902353UA (en) Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
MX2022013590A (en) Methods and compositions for treatment of attention deficit disorder.
IN2015DN03826A (en)
NZ609201A (en) Means and methods for treating dlbcl
EA201401028A1 (en) PHARMACEUTICAL COMPOSITION, CONTAINING EMAGOGLIFLOZIN AND MEDICINES FOR OBESITY
MX2015016983A (en) Nuclear transport modulators and uses thereof.
MX2013002488A (en) Methods and compositions for delivering interleukin-1 receptor antagonist.
UA118085C2 (en) Nuclear transport modulators and uses thereof
JP2014141525A5 (en)
TN2012000335A1 (en) Antibody formulation and therapeutic regimens
MX2015000337A (en) Etanercept formulations exhibiting marked reduction in sub-visible particles.
IL228600A0 (en) (alpha- substituted aralkylamino and heteroarylalkylamino) pyrimidinyl and 1,3,5 -triazinyl benzimidazoles, pharmaceutical compositions containing them, and these compounds for use in treating proliferative diseases
MX2021016109A (en) Methods and compositions particularly for treatment of attention deficit disorder.
PH12014502366B1 (en) Injectable formulation
MX344846B (en) Combination of active loaded granules with additional actives.
EA201400737A1 (en) DELIVERY SYSTEM ON THE BASIS OF A PELLET SET WITH IMMEDIATE release of an ACTIVE SUBSTANCE
CR20140358A (en) DRUG ADMINISTRATION SYSTEM
MX2014012164A (en) Compositions containing probiotics and a bee pollen/clay complex, preparation method thereof and uses of same in nutrition and therapeutics.
EA201591415A1 (en) PHARMACEUTICAL COMPOSITIONS, INCLUDING MULTICOMPONENT CRYSTALLINE PARTICLES, SUITABLE FOR USE IN INHALATION THERAPY
MX2016002930A (en) Liver x receptor (lxr) modulators.
MX2016011706A (en) Progesterone formulations.
TR201908937T4 (en) Indanyl urea compounds that inhibit P38 MAP kinase.
WO2014078506A3 (en) Cell delivery device and system with anti-clumping feature and methods for pelvic tissue treatment
PH12015501533A1 (en) Controlled release formulations of lorazepam
MX2015004940A (en) Treatment of depression and ptsd.