JP2017504315A5 - - Google Patents
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- Publication number
- JP2017504315A5 JP2017504315A5 JP2016540965A JP2016540965A JP2017504315A5 JP 2017504315 A5 JP2017504315 A5 JP 2017504315A5 JP 2016540965 A JP2016540965 A JP 2016540965A JP 2016540965 A JP2016540965 A JP 2016540965A JP 2017504315 A5 JP2017504315 A5 JP 2017504315A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- nucleic acid
- capture
- enclosure
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims 67
- 238000000034 method Methods 0.000 claims 34
- 150000007523 nucleic acids Chemical class 0.000 claims 26
- 102000039446 nucleic acids Human genes 0.000 claims 26
- 108020004707 nucleic acids Proteins 0.000 claims 26
- 238000002955 isolation Methods 0.000 claims 25
- 239000000463 material Substances 0.000 claims 25
- 230000002934 lysing effect Effects 0.000 claims 16
- 239000000758 substrate Substances 0.000 claims 7
- 239000012528 membrane Substances 0.000 claims 6
- 238000004720 dielectrophoresis Methods 0.000 claims 5
- 238000012360 testing method Methods 0.000 claims 5
- 239000007788 liquid Substances 0.000 claims 4
- 230000004913 activation Effects 0.000 claims 2
- 210000003855 cell nucleus Anatomy 0.000 claims 2
- 230000009089 cytolysis Effects 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 238000009792 diffusion process Methods 0.000 claims 2
- 210000003463 organelle Anatomy 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019188011A JP6954972B2 (ja) | 2013-12-18 | 2019-10-11 | マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14/133,361 US20150166326A1 (en) | 2013-12-18 | 2013-12-18 | Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device |
| US14/133,361 | 2013-12-18 | ||
| PCT/US2014/071323 WO2015095623A1 (en) | 2013-12-18 | 2014-12-18 | Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019188011A Division JP6954972B2 (ja) | 2013-12-18 | 2019-10-11 | マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017504315A JP2017504315A (ja) | 2017-02-09 |
| JP2017504315A5 true JP2017504315A5 (cg-RX-API-DMAC7.html) | 2018-02-01 |
| JP6603663B2 JP6603663B2 (ja) | 2019-11-06 |
Family
ID=53367562
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016540965A Active JP6603663B2 (ja) | 2013-12-18 | 2014-12-18 | マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法 |
| JP2019188011A Active JP6954972B2 (ja) | 2013-12-18 | 2019-10-11 | マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019188011A Active JP6954972B2 (ja) | 2013-12-18 | 2019-10-11 | マイクロ流体デバイスにおける個々の生体細胞から特定の核酸材料を捕捉する方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20150166326A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3083980B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP6603663B2 (cg-RX-API-DMAC7.html) |
| KR (2) | KR102448505B1 (cg-RX-API-DMAC7.html) |
| DK (1) | DK3083980T3 (cg-RX-API-DMAC7.html) |
| SG (2) | SG10201902353UA (cg-RX-API-DMAC7.html) |
| WO (1) | WO2015095623A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9403172B2 (en) * | 2012-11-08 | 2016-08-02 | Berkeley Lights, Inc. | Circuit based optoelectronic tweezers |
| US20150166326A1 (en) * | 2013-12-18 | 2015-06-18 | Berkeley Lights, Inc. | Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device |
| WO2016172623A1 (en) | 2015-04-22 | 2016-10-27 | Berkeley Lights, Inc. | Manipulation of cell nuclei in a micro-fluidic device |
| IL299366A (en) | 2015-11-23 | 2023-02-01 | Berkeley Lights Inc | In situ-generated microfluidic isolation structures, kits and methods of use thereof |
| TWI808934B (zh) | 2015-12-31 | 2023-07-21 | 美商伯克利之光生命科技公司 | 經工程化以表現促發炎多肽之腫瘤浸潤細胞 |
| WO2017160991A1 (en) | 2016-03-16 | 2017-09-21 | Lavieu Gregory G | Methods, systems and devices for selection and generation of genome edited clones |
| CN109311930B (zh) * | 2016-03-31 | 2023-02-24 | 伯克利之光生命科技公司 | 核酸稳定试剂、试剂盒及其使用方法 |
| CN110248733B (zh) * | 2016-12-01 | 2022-01-11 | 伯克利之光生命科技公司 | 用于对微物体成像的设备、系统和方法 |
| CA3045333A1 (en) * | 2016-12-01 | 2018-06-07 | Berkeley Lights, Inc. | Automated detection and repositioning of micro-objects in microfluidic devices |
| EP3551745A4 (en) | 2016-12-12 | 2020-07-15 | Xcella Biosciences, Inc. | SCREENING METHODS AND SYSTEMS USING MICROCAPILLARY NETWORKS |
| CA3048645A1 (en) | 2016-12-30 | 2018-07-05 | The Regents Of The University Of California | Methods for selection and generation of genome edited t cells |
| US11156626B2 (en) | 2016-12-30 | 2021-10-26 | xCella Biosciences, Inc. | Multi-stage sample recovery system |
| WO2018207454A1 (ja) * | 2017-05-12 | 2018-11-15 | 株式会社ニコン | 対象物操作装置、及び対象物操作方法 |
| WO2018226900A2 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | A htp genomic engineering platform for improving fungal strains |
| KR20210018219A (ko) | 2018-06-06 | 2021-02-17 | 지머젠 인코포레이티드 | 발효 및 생산 중 곰팡이 형태를 제어하기 위한 신호 전달에 포함된 유전자의 조작 |
| KR20210078510A (ko) | 2018-10-11 | 2021-06-28 | 버클리 라잇츠, 인크. | 최적화된 단백질 생성의 확인을 위한 시스템 및 방법, 및 이를 위한 키트 |
| EP3883692A4 (en) * | 2018-11-19 | 2022-08-31 | Berkeley Lights, Inc. | MICROFLUID DEVICE WITH PROGRAMMABLE SWITCHING ELEMENTS |
| US12318775B2 (en) | 2018-12-06 | 2025-06-03 | xCella Biosciences, Inc. | Lateral loading of microcapillary arrays |
| US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6685812B2 (en) * | 2001-01-09 | 2004-02-03 | The Regents Of The University Of California | Movement of particles using sequentially activated dielectrophoretic particle trapping |
| US7312085B2 (en) * | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
| US6958132B2 (en) | 2002-05-31 | 2005-10-25 | The Regents Of The University Of California | Systems and methods for optical actuation of microfluidics based on opto-electrowetting |
| AU2003299541A1 (en) * | 2002-10-02 | 2004-05-25 | California Institute Of Technology | Microfluidic nucleic acid analysis |
| WO2004065618A2 (en) * | 2003-01-16 | 2004-08-05 | Thermogenic Imaging | Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers |
| US20050142565A1 (en) * | 2003-12-30 | 2005-06-30 | Agency For Science, Technology And Research | Nucleic acid purification chip |
| JP2007537729A (ja) * | 2004-04-12 | 2007-12-27 | ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア | 微粒子及び細胞の操作用の光電子ピンセット |
| EP3029135B1 (en) * | 2005-07-07 | 2021-03-17 | The Regents of the University of California | Apparatus for cell culture array |
| WO2007102839A2 (en) * | 2005-10-27 | 2007-09-13 | Applera Corporation | Optoelectronic separation of biomolecules |
| CN101415813B (zh) * | 2006-02-03 | 2013-04-10 | 微芯片生物工艺学股份有限公司 | 微流体装置 |
| US9040288B2 (en) * | 2006-03-24 | 2015-05-26 | Handylab, Inc. | Integrated system for processing microfluidic samples, and method of using the same |
| US7439014B2 (en) * | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
| WO2008119066A1 (en) | 2007-03-28 | 2008-10-02 | The Regents Of The University Of California | Single-sided lateral-field and phototransistor-based optoelectronic tweezers |
| KR101228308B1 (ko) * | 2007-05-23 | 2013-01-31 | 삼성전자주식회사 | 미세유동 칩을 이용한 디스크형 미세유동장치 및 생체물질마이크로어레이 칩을 이용한 디스크형 미세유동장치 |
| ATE554859T1 (de) * | 2007-05-24 | 2012-05-15 | Univ California | Integrierte fluidische vorrichtungen mit magnetischer sortierung |
| US9533306B2 (en) | 2010-08-02 | 2017-01-03 | The Regents Of The University Of California | Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns |
| EP2606154B1 (en) * | 2010-08-20 | 2019-09-25 | Integenx Inc. | Integrated analysis system |
| US9506845B2 (en) * | 2012-02-29 | 2016-11-29 | Fluidigm Corporation | Methods, systems and devices for multiple single-cell capturing and processing using microfluidics |
| US9857333B2 (en) | 2012-10-31 | 2018-01-02 | Berkeley Lights, Inc. | Pens for biological micro-objects |
| US9403172B2 (en) | 2012-11-08 | 2016-08-02 | Berkeley Lights, Inc. | Circuit based optoelectronic tweezers |
| US20150166326A1 (en) * | 2013-12-18 | 2015-06-18 | Berkeley Lights, Inc. | Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device |
-
2013
- 2013-12-18 US US14/133,361 patent/US20150166326A1/en not_active Abandoned
-
2014
- 2014-12-18 DK DK14872873.6T patent/DK3083980T3/da active
- 2014-12-18 SG SG10201902353UA patent/SG10201902353UA/en unknown
- 2014-12-18 KR KR1020217004925A patent/KR102448505B1/ko active Active
- 2014-12-18 KR KR1020167018502A patent/KR102220001B1/ko active Active
- 2014-12-18 SG SG11201604907SA patent/SG11201604907SA/en unknown
- 2014-12-18 WO PCT/US2014/071323 patent/WO2015095623A1/en not_active Ceased
- 2014-12-18 JP JP2016540965A patent/JP6603663B2/ja active Active
- 2014-12-18 EP EP14872873.6A patent/EP3083980B1/en active Active
-
2019
- 2019-10-11 JP JP2019188011A patent/JP6954972B2/ja active Active
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