JP6545676B2 - 改善された代謝能力を有する細菌 - Google Patents

改善された代謝能力を有する細菌 Download PDF

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JP6545676B2
JP6545676B2 JP2016530177A JP2016530177A JP6545676B2 JP 6545676 B2 JP6545676 B2 JP 6545676B2 JP 2016530177 A JP2016530177 A JP 2016530177A JP 2016530177 A JP2016530177 A JP 2016530177A JP 6545676 B2 JP6545676 B2 JP 6545676B2
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coli
strain
modified
gene
parent
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JP2016536993A (ja
JP2016536993A5 (enExample
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フレデリック アール． ブラットナー，
フレデリック アール． ブラットナー，
デイビッド エー． フリッシュ，
デイビッド エー． フリッシュ，
ロバート イー． ノビー，
ロバート イー． ノビー，
テランス エム． ヘンカー，
テランス エム． ヘンカー，
エリック エー． ステフェン，
エリック エー． ステフェン，
ホセ エー． サンチェス，
ホセ エー． サンチェス，
クリストファー アール． ブラットナー，
クリストファー アール． ブラットナー，
ヒュンシク チョイ，
ヒュンシク チョイ，
ジォルジュ ポスファイ，
ジォルジュ ポスファイ，
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スカラブ ゲノミクス， エルエルシー
スカラブ ゲノミクス， エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2016530177A 2013-11-14 2014-11-13 改善された代謝能力を有する細菌 Active JP6545676B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361904298P 2013-11-14 2013-11-14
US61/904,298 2013-11-14
PCT/US2014/065545 WO2015073720A1 (en) 2013-11-14 2014-11-13 Bacteria with improved metabolic capacity

Publications (3)

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JP2016536993A JP2016536993A (ja) 2016-12-01
JP2016536993A5 JP2016536993A5 (enExample) 2017-12-28
JP6545676B2 true JP6545676B2 (ja) 2019-07-17

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JP2016530177A Active JP6545676B2 (ja) 2013-11-14 2014-11-13 改善された代謝能力を有する細菌

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US (2) US9902965B2 (enExample)
EP (1) EP3068887B1 (enExample)
JP (1) JP6545676B2 (enExample)
WO (1) WO2015073720A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016242996B2 (en) 2015-04-02 2021-02-18 Scarab Genomics, Llc Materials and methods for extended continuous flow fermentation of reduced genome bacteria
AU2017233862B2 (en) 2016-03-15 2023-04-13 Rnaissance Ag Llc Methods and compositions for increased double stranded RNA production
AU2018329671B2 (en) 2017-09-05 2023-12-07 Scarab Genomics, Llc Methods and compositions for improved expression of recombinant proteins
CN108587992B (zh) * 2018-04-10 2022-03-15 安徽大学 一种高效分泌表达抗VEGF-Fab抗体片段的高产菌株及其构建方法
CA3142362A1 (en) * 2019-05-30 2020-12-03 Rnaissance Ag Llc Improved methods and compositions for increased double stranded rna production
CN115698276A (zh) * 2020-04-15 2023-02-03 阿拉斯加大学安克雷奇分校 用于生产异丁烯的方法和组合物
US12168679B2 (en) 2020-11-10 2024-12-17 Northwestern University Modified bacteria for enhanced bioproduction and methods of using the same
WO2024158973A2 (en) 2023-01-25 2024-08-02 Novel Biotechnology Inc. Expression system for product manufacturing

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5578464A (en) 1989-10-31 1996-11-26 Schering Corporation E. coli secretory strains
US5747662A (en) 1995-03-01 1998-05-05 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
US6022952A (en) 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US8119365B2 (en) 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US8039243B2 (en) 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US7303906B2 (en) 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
US7300776B2 (en) 2004-04-26 2007-11-27 Ajinomoto Co., Inc. L-amino acid-producing bacterium and a method for producing L-amino acid
AU2005308101A1 (en) * 2004-11-26 2006-06-01 Kyowa Hakko Kirin Co., Ltd. Industrially useful microorganism
US8178339B2 (en) 2005-08-20 2012-05-15 Scarab Genomics Llc Reduced genome E. coli
JP2009165355A (ja) 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
WO2007140816A1 (en) * 2006-06-09 2007-12-13 Metabolic Explorer Glycolic acid production by fermentation from renewable resources
US8367380B2 (en) 2008-02-22 2013-02-05 Scarab Genomics Llc Compositions and methods for amino acid biosynthesis
WO2009116566A1 (ja) * 2008-03-18 2009-09-24 協和発酵キリン株式会社 工業的に有用な微生物
CN103189517A (zh) 2010-08-27 2013-07-03 代谢探索者公司 用经修饰的微生物改善乙醇酸发酵产生
WO2013059595A1 (en) * 2011-10-20 2013-04-25 Scarab Genomics, Llc Reduced genome bacteria with improved genetic stability
WO2015134402A1 (en) * 2014-03-03 2015-09-11 Scarab Genomics, Llc Enhanced production of recombinant crm197 in e. coli

Also Published As

Publication number Publication date
EP3068887A1 (en) 2016-09-21
US20160298126A1 (en) 2016-10-13
WO2015073720A1 (en) 2015-05-21
EP3068887B1 (en) 2019-10-30
JP2016536993A (ja) 2016-12-01
US9902965B2 (en) 2018-02-27
US20180208933A1 (en) 2018-07-26
EP3068887A4 (en) 2017-05-10

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