JP6545153B2 - 発現方法 - Google Patents

発現方法 Download PDF

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Publication number
JP6545153B2
JP6545153B2 JP2016511126A JP2016511126A JP6545153B2 JP 6545153 B2 JP6545153 B2 JP 6545153B2 JP 2016511126 A JP2016511126 A JP 2016511126A JP 2016511126 A JP2016511126 A JP 2016511126A JP 6545153 B2 JP6545153 B2 JP 6545153B2
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Japan
Prior art keywords
chinese hamster
hamster ovary
sequence
cells
expression
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JP2016511126A
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English (en)
Japanese (ja)
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JP2016517691A5 (cg-RX-API-DMAC10.html
JP2016517691A (ja
Inventor
サンダース，フェイ・ルイーズ
ドッズ，アンナ・ルイーズ
ベイヤード，アデライン・マリー・ジェラルディン
カラ，ブペンドラ・ヴァラブ
Original Assignee
フジフィルム・ダイオシンス・バイオテクノロジーズ ・ユーケイ・リミテッド
フジフィルム・ダイオシンス・バイオテクノロジーズ ・ユーケイ・リミテッド
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Priority claimed from GB201308017A external-priority patent/GB201308017D0/en
Priority claimed from GB201320339A external-priority patent/GB201320339D0/en
Application filed by フジフィルム・ダイオシンス・バイオテクノロジーズ ・ユーケイ・リミテッド, フジフィルム・ダイオシンス・バイオテクノロジーズ ・ユーケイ・リミテッド filed Critical フジフィルム・ダイオシンス・バイオテクノロジーズ ・ユーケイ・リミテッド
Publication of JP2016517691A publication Critical patent/JP2016517691A/ja
Publication of JP2016517691A5 publication Critical patent/JP2016517691A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3076—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties
    • C07K16/3092—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties against tumour-associated mucins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
JP2016511126A 2013-05-03 2014-04-29 発現方法 Active JP6545153B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB201308017A GB201308017D0 (en) 2013-05-03 2013-05-03 Expression process
GB1308017.1 2013-05-03
GB201320339A GB201320339D0 (en) 2013-11-18 2013-11-18 Expression Process
GB1320339.3 2013-11-18
PCT/GB2014/000165 WO2014177826A1 (en) 2013-05-03 2014-04-29 Expression process

Publications (3)

Publication Number Publication Date
JP2016517691A JP2016517691A (ja) 2016-06-20
JP2016517691A5 JP2016517691A5 (cg-RX-API-DMAC10.html) 2017-06-08
JP6545153B2 true JP6545153B2 (ja) 2019-07-17

Family

ID=50639794

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016511126A Active JP6545153B2 (ja) 2013-05-03 2014-04-29 発現方法

Country Status (12)

Country Link
US (3) US9873891B2 (cg-RX-API-DMAC10.html)
EP (1) EP2992104B1 (cg-RX-API-DMAC10.html)
JP (1) JP6545153B2 (cg-RX-API-DMAC10.html)
KR (1) KR102180660B1 (cg-RX-API-DMAC10.html)
CN (2) CN105392889A (cg-RX-API-DMAC10.html)
BR (1) BR112015027373A2 (cg-RX-API-DMAC10.html)
CA (1) CA2910877C (cg-RX-API-DMAC10.html)
DK (1) DK2992104T3 (cg-RX-API-DMAC10.html)
ES (1) ES2732907T3 (cg-RX-API-DMAC10.html)
RU (1) RU2731717C2 (cg-RX-API-DMAC10.html)
SG (1) SG11201508447TA (cg-RX-API-DMAC10.html)
WO (1) WO2014177826A1 (cg-RX-API-DMAC10.html)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3323826A1 (en) * 2016-11-21 2018-05-23 Danmarks Tekniske Universitet Native chinese hamster ovary cell secretion signal peptides for production of recombinant polypeptides
EP3725877A4 (en) 2017-12-11 2021-02-17 FUJIFILM Corporation ANIMAL CELLS AS WELL AS A METHOD FOR MANUFACTURING THE SAME, AND A METHOD FOR MANUFACTURING TARGET PROTEIN
JP7123168B2 (ja) 2018-12-11 2022-08-22 富士フイルム株式会社 動物細胞、動物細胞の製造方法および目的タンパク質の製造方法
CN109837277A (zh) * 2019-03-01 2019-06-04 深圳生生凡非基因技术有限公司 一种cpgk嵌合型启动子、腺相关病毒载体及其应用
JP7352650B2 (ja) 2019-11-29 2023-09-28 富士フイルム株式会社 細胞培養方法、抗体製造方法、有機酸除去方法、及び、抗体
EP4276167A1 (en) 2021-01-07 2023-11-15 FUJIFILM Corporation Cell selection method, device and device system
WO2022196710A1 (ja) 2021-03-17 2022-09-22 富士フイルム株式会社 細胞培養方法、及び有用物質の製造方法
CN112980852B (zh) * 2021-05-18 2021-08-13 北京华芢生物技术有限公司 新型冠状病毒b.1.351南非突变株rbd的基因及其应用
MX2023014069A (es) 2021-05-27 2024-03-15 Janssen Biotech Inc Composiciones y métodos para el tratamiento del cáncer de próstata.
WO2023054556A1 (ja) 2021-09-30 2023-04-06 富士フイルム株式会社 細胞培養方法、及び有用物質の製造方法
CN118541491A (zh) 2021-12-15 2024-08-23 富士胶片株式会社 产物的制造方法及细胞培养装置
JPWO2023190300A1 (cg-RX-API-DMAC10.html) 2022-03-28 2023-10-05
EP4502149A4 (en) 2022-03-31 2026-03-11 Fujifilm Corp PRODUCT MANUFACTURING PROCESS AND PRODUCT
CN119654417A (zh) 2022-06-10 2025-03-18 富士胶片戴奥辛思生物技术英国有限公司 细胞的制备方法、异源多聚体蛋白质的制造方法、双特异性抗体的制造方法、载体组、哺乳动物细胞、cho细胞和细胞池的制备方法
JPWO2024071373A1 (cg-RX-API-DMAC10.html) 2022-09-29 2024-04-04
JPWO2024248120A1 (cg-RX-API-DMAC10.html) 2023-05-31 2024-12-05
EP4722338A1 (en) 2023-05-31 2026-04-08 FUJIFILM Corporation Cell culture method and product producing method
CN121175432A (zh) 2023-05-31 2025-12-19 富士胶片株式会社 生产物的生产方法
WO2025244971A1 (en) 2024-05-20 2025-11-27 Janssen Biotech, Inc. Compositions and methods for the treatment of prostate cancer

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7071172B2 (en) * 2002-04-30 2006-07-04 The University Of North Carolina At Chapel Hill Secretion signal vectors
US7186699B2 (en) * 2003-06-03 2007-03-06 Cell Genesys, Inc. Method for treating cancer by vector-mediated delivery of one or more anti-angiogenic or pro-apoptotic genes
US8029783B2 (en) * 2005-02-02 2011-10-04 Genentech, Inc. DR5 antibodies and articles of manufacture containing same
EP1739179A1 (en) * 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
ES2474940T3 (es) * 2006-12-28 2014-07-10 Janssen Biotech, Inc. Métodos y vectores que generan inmunoglobulinas sialiladas
KR101618767B1 (ko) * 2008-09-02 2016-05-09 한국생명공학연구원 외래단백질을 고효율로 생산하는 리더서열
ES2740051T3 (es) * 2008-12-31 2020-02-05 Bharat Serums & Vaccines Ltd Anticuerpos monoclonales anti-RHD
NZ594514A (en) 2009-03-05 2013-06-28 Abbott Lab Interleukin-17 BINDING PROTEINS
GB0905331D0 (en) * 2009-03-27 2009-05-13 Avecia Biolog Ltd Expression process
KR101420274B1 (ko) * 2011-06-13 2014-07-21 주식회사 종근당 Ｃｓｐ－ｂ ５＇ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법

Also Published As

Publication number Publication date
CA2910877C (en) 2021-08-03
RU2731717C2 (ru) 2020-09-08
RU2015151626A (ru) 2017-06-08
KR102180660B1 (ko) 2020-11-19
KR20160003705A (ko) 2016-01-11
JP2016517691A (ja) 2016-06-20
CN114395584B (zh) 2025-01-28
CN114395584A (zh) 2022-04-26
CN105392889A (zh) 2016-03-09
ES2732907T3 (es) 2019-11-26
RU2015151626A3 (cg-RX-API-DMAC10.html) 2018-03-20
US9873891B2 (en) 2018-01-23
BR112015027373A2 (pt) 2017-08-29
SG11201508447TA (en) 2015-11-27
WO2014177826A1 (en) 2014-11-06
CA2910877A1 (en) 2014-11-06
EP2992104B1 (en) 2019-04-17
US20220213500A1 (en) 2022-07-07
US20180127777A1 (en) 2018-05-10
EP2992104A1 (en) 2016-03-09
US20160076051A1 (en) 2016-03-17
DK2992104T3 (da) 2019-07-15
US11306323B2 (en) 2022-04-19

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