JP6495440B2 - 核酸リガンドの構造変化に基づくspfsバイオセンサー - Google Patents
核酸リガンドの構造変化に基づくspfsバイオセンサー Download PDFInfo
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- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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Description
プリズムと、
前記プリズム上に形成された金属薄膜であって、所定波長の入射光により所定角度でプリズムが照射されると、表面近くに電場を発生するよう構成され、前記電場が、前記金属薄膜の表面に隣接する蛍光クエンチ領域と、前記蛍光クエンチ領域よりも更に表面から離れた位置の蛍光増強領域とを形成する、金属薄膜と、
一端が前記金属薄膜の表面に固定され、他端が蛍光マーカーで修飾された核酸分子であって、標的が結合すると折りたたまれた状態から伸長した状態へ、又は標的が結合すると伸長した状態から折りたたまれた状態へ構造が変化する、核酸分子と、
前記核酸分子が、金属薄膜の表面から離れる方向に伸長するように前記蛍光マーカー又は前記核酸分子に磁力を加える手段と、
を備え、
前記核酸分子が伸長状態にあるとき、前記蛍光マーカーは、前記蛍光増強領域に位置して第1の蛍光シグナルを発し、且つ、前記核酸分子が折りたたまれた状態にあるとき、前記蛍光マーカーは、蛍光クエンチ領域に位置して蛍光シグナルを発さないか、又は第1の蛍光シグナルよりも弱い第2の蛍光シグナルを発し、
前記蛍光マーカーが常磁性を有するか、又は前記核酸分子が、前記蛍光マーカーで修飾された端部において常磁性を有する別の分子で修飾されている。
シグナル = o f(d) * h(d) dd
ノイズ = o f(d) * g(d) dd
Claims (6)
- 光学バイオセンサーであって、
プリズムと、
前記プリズム上に形成された金属薄膜であって、所定波長の入射光により所定角度でプリズムが照射されると、表面近くに電場を発生するよう構成され、前記電場が、前記金属薄膜の表面に隣接する蛍光クエンチ領域と、前記蛍光クエンチ領域よりも更に表面から離れた位置の蛍光増強領域とを形成する、金属薄膜と、
一端が前記金属薄膜の表面に固定され、他端が蛍光マーカーで修飾された核酸分子であって、標的が結合すると折りたたまれた状態から伸長した状態へ、又は標的が結合すると伸長した状態から折りたたまれた状態へ構造が変化する、核酸分子と、
前記核酸分子が、金属薄膜の表面から離れる方向に伸長するように前記蛍光マーカー又は前記核酸分子に磁力を加える手段と、
を備え、
前記核酸分子が伸長状態にあるとき、前記蛍光マーカーは、前記蛍光増強領域に位置して第1の蛍光シグナルを発し、且つ、前記核酸分子が折りたたまれた状態にあるとき、前記蛍光マーカーは、蛍光クエンチ領域に位置して蛍光シグナルを発さないか、又は第1の蛍光シグナルよりも弱い第2の蛍光シグナルを発し、
前記蛍光マーカーが常磁性を有するか、又は前記核酸分子が、前記蛍光マーカーで修飾された端部において常磁性を有する別の分子で修飾されている、光学バイオセンサー。 - 前記バイオセンサーが、表面プラズモン励起増強蛍光分光センサーである、請求項1に記載の光学バイオセンサー。
- 前記蛍光マーカーが、前記金属薄膜の表面に導入される試料媒質の密度よりも高い又は低い密度を有する、請求項1または2に記載の光学バイオセンサー。
- 前記核酸分子が、前記蛍光マーカーで修飾された端部においてマイクロバブルで修飾されている、請求項1〜3のいずれかに記載の光学バイオセンサー。
- 請求項1〜4のいずれかの光学バイオセンサーの使用方法であって、
前記バイオセンサーの金属薄膜表面に試料を導入する工程と、
前記バイオセンサーから蛍光シグナルを測定する工程と、
からなる方法。 - 前記核酸分子が金属薄膜の表面から離れる方向に伸長するように、前記蛍光マーカー又は前記核酸分子に外力を加える工程、を更に備える請求項5に記載の方法。
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PCT/US2015/047988 WO2016040059A1 (en) | 2014-09-10 | 2015-09-01 | Spfs biosensor based on nucleic acid ligand structural change |
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WO2016040059A1 (en) | 2016-03-17 |
JP2017534844A (ja) | 2017-11-24 |
US10180425B2 (en) | 2019-01-15 |
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