JP6469371B2 - 人工多能性幹細胞(iPS細胞)から成る胚様体に複数の外来遺伝子を発現させる方法 - Google Patents
人工多能性幹細胞(iPS細胞)から成る胚様体に複数の外来遺伝子を発現させる方法 Download PDFInfo
- Publication number
- JP6469371B2 JP6469371B2 JP2014132622A JP2014132622A JP6469371B2 JP 6469371 B2 JP6469371 B2 JP 6469371B2 JP 2014132622 A JP2014132622 A JP 2014132622A JP 2014132622 A JP2014132622 A JP 2014132622A JP 6469371 B2 JP6469371 B2 JP 6469371B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- gene
- ips cells
- foreign
- ips
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014132622A JP6469371B2 (ja) | 2014-06-27 | 2014-06-27 | 人工多能性幹細胞(iPS細胞)から成る胚様体に複数の外来遺伝子を発現させる方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014132622A JP6469371B2 (ja) | 2014-06-27 | 2014-06-27 | 人工多能性幹細胞(iPS細胞)から成る胚様体に複数の外来遺伝子を発現させる方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016010330A JP2016010330A (ja) | 2016-01-21 |
| JP2016010330A5 JP2016010330A5 (enExample) | 2017-08-10 |
| JP6469371B2 true JP6469371B2 (ja) | 2019-02-13 |
Family
ID=55227519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014132622A Active JP6469371B2 (ja) | 2014-06-27 | 2014-06-27 | 人工多能性幹細胞(iPS細胞)から成る胚様体に複数の外来遺伝子を発現させる方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6469371B2 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110868853A (zh) * | 2017-04-26 | 2020-03-06 | 纪念斯隆-凯特琳癌症中心 | 即用型冷冻保存细胞 |
| CN107164527B (zh) * | 2017-06-29 | 2018-04-20 | 杭州观梓健康科技有限公司 | 一种筛选参与多能干细胞体外定向分化调节因子的方法 |
| CN111139218A (zh) * | 2020-01-16 | 2020-05-12 | 协和干细胞基因工程有限公司 | 用诱导多能干细胞或胚胎干细胞快速高效制备拟胚体的方法 |
-
2014
- 2014-06-27 JP JP2014132622A patent/JP6469371B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2016010330A (ja) | 2016-01-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12496274B2 (en) | Compositions and methods for compartment-specific cargo delivery | |
| BR112020016570A2 (pt) | Composições e métodos para liberação de proteína de membrana | |
| CN108588023B (zh) | 一种生产嵌合抗原受体修饰的γδT细胞的方法 | |
| EP1559782A1 (en) | Human artificial chromosome (hac) vector | |
| KR20160145814A (ko) | 세포 생산성을 향상시키는 마이크로rna | |
| ES2547700T3 (es) | Nueva línea de células humanas permanente | |
| WO2011071476A2 (en) | Compositions and methods for engineering cells | |
| EP2785832A1 (en) | Haploid cells | |
| CN107893073A (zh) | 一种筛选谷氨酰胺合成酶缺陷型hek293细胞株的方法 | |
| KR102428606B1 (ko) | 향상된 유전자 전달 방법 | |
| JP2021048875A (ja) | 膵内分泌細胞の製造方法、及び分化転換剤 | |
| Sauter et al. | Induced neurons for the study of neurodegenerative and neurodevelopmental disorders | |
| Grossi et al. | Generation of knockout human primary keratinocytes by CRISPR/Cas9 | |
| JP2016010330A (ja) | 人工多能性幹細胞(iPS細胞)から成る胚様体に複数の外来遺伝子を発現させる方法 | |
| CN109868286A (zh) | 一种慢病毒载体、慢病毒及其制备方法和应用 | |
| Kumari et al. | Transgenic animals in biotechnology | |
| WO2022243320A1 (en) | Method for producing a biomolecule by a cell | |
| Wang et al. | Laboratory-Engineered Glioblastoma Organoid Culture and Drug Screening | |
| KR102091511B1 (ko) | 줄기세포 분화 효율 촉진 방법 | |
| Siddiqui et al. | A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells | |
| EP4339272A1 (en) | Systems and methods for developing and optimizing cell culture processes | |
| KR101348295B1 (ko) | Bmp2 발현 세포를 무혈청 배지에서 단일클론으로 배양하는 방법 | |
| US20230272346A1 (en) | Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure | |
| WO2025060088A1 (zh) | 一种免疫细胞的制备方法 | |
| JP2026512866A (ja) | 遺伝子発現構築物のためのスペーサー |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170623 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20170623 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20180626 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180820 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20181225 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20190116 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6469371 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |