JP2016010330A5 - - Google Patents
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- JP2016010330A5 JP2016010330A5 JP2014132622A JP2014132622A JP2016010330A5 JP 2016010330 A5 JP2016010330 A5 JP 2016010330A5 JP 2014132622 A JP2014132622 A JP 2014132622A JP 2014132622 A JP2014132622 A JP 2014132622A JP 2016010330 A5 JP2016010330 A5 JP 2016010330A5
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- JP
- Japan
- Prior art keywords
- ips cells
- foreign
- cells
- gene
- episomal vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004027 cell Anatomy 0.000 claims 28
- 108090000623 proteins and genes Proteins 0.000 claims 20
- 238000000034 method Methods 0.000 claims 13
- 210000002242 embryoid body Anatomy 0.000 claims 9
- 108700008625 Reporter Genes Proteins 0.000 claims 8
- 239000003814 drug Substances 0.000 claims 8
- 229940079593 drug Drugs 0.000 claims 8
- 238000012136 culture method Methods 0.000 claims 7
- 238000012258 culturing Methods 0.000 claims 5
- 239000003550 marker Substances 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- GRRNUXAQVGOGFE-UHFFFAOYSA-N Hygromycin-B Natural products OC1C(NC)CC(N)C(O)C1OC1C2OC3(C(C(O)C(O)C(C(N)CO)O3)O)OC2C(O)C(CO)O1 GRRNUXAQVGOGFE-UHFFFAOYSA-N 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 4
- -1 cationic lipid Chemical class 0.000 claims 4
- GRRNUXAQVGOGFE-NZSRVPFOSA-N hygromycin B Chemical compound O[C@@H]1[C@@H](NC)C[C@@H](N)[C@H](O)[C@H]1O[C@H]1[C@H]2O[C@@]3([C@@H]([C@@H](O)[C@@H](O)[C@@H](C(N)CO)O3)O)O[C@H]2[C@@H](O)[C@@H](CO)O1 GRRNUXAQVGOGFE-NZSRVPFOSA-N 0.000 claims 4
- 229940097277 hygromycin b Drugs 0.000 claims 4
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 4
- 239000012096 transfection reagent Substances 0.000 claims 4
- 230000003068 static effect Effects 0.000 claims 3
- 108091023040 Transcription factor Proteins 0.000 claims 2
- 102000040945 Transcription factor Human genes 0.000 claims 2
- 108010048367 enhanced green fluorescent protein Proteins 0.000 claims 2
- 102100035961 Hematopoietically-expressed homeobox protein HHEX Human genes 0.000 claims 1
- 102100029284 Hepatocyte nuclear factor 3-beta Human genes 0.000 claims 1
- 101001021503 Homo sapiens Hematopoietically-expressed homeobox protein HHEX Proteins 0.000 claims 1
- 101001062347 Homo sapiens Hepatocyte nuclear factor 3-beta Proteins 0.000 claims 1
- 101000819074 Homo sapiens Transcription factor GATA-4 Proteins 0.000 claims 1
- 102100021380 Transcription factor GATA-4 Human genes 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 230000004069 differentiation Effects 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000002062 proliferating effect Effects 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014132622A JP6469371B2 (ja) | 2014-06-27 | 2014-06-27 | 人工多能性幹細胞（ｉＰＳ細胞）から成る胚様体に複数の外来遺伝子を発現させる方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014132622A JP6469371B2 (ja) | 2014-06-27 | 2014-06-27 | 人工多能性幹細胞（ｉＰＳ細胞）から成る胚様体に複数の外来遺伝子を発現させる方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016010330A JP2016010330A (ja) | 2016-01-21 |
| JP2016010330A5 true JP2016010330A5 (enExample) | 2017-08-10 |
| JP6469371B2 JP6469371B2 (ja) | 2019-02-13 |
Family
ID=55227519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014132622A Active JP6469371B2 (ja) | 2014-06-27 | 2014-06-27 | 人工多能性幹細胞（ｉＰＳ細胞）から成る胚様体に複数の外来遺伝子を発現させる方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6469371B2 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3061165A1 (en) * | 2017-04-26 | 2018-11-01 | Memorial Sloan-Kettering Cancer Center | Ready-to-use cryopreserved cells |
| CN107164527B (zh) * | 2017-06-29 | 2018-04-20 | 杭州观梓健康科技有限公司 | 一种筛选参与多能干细胞体外定向分化调节因子的方法 |
| CN111139218A (zh) * | 2020-01-16 | 2020-05-12 | 协和干细胞基因工程有限公司 | 用诱导多能干细胞或胚胎干细胞快速高效制备拟胚体的方法 |
-
2014
- 2014-06-27 JP JP2014132622A patent/JP6469371B2/ja active Active
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