JP6466966B2 - スポットアレイ基板の製造方法 - Google Patents
スポットアレイ基板の製造方法 Download PDFInfo
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- JP6466966B2 JP6466966B2 JP2016561445A JP2016561445A JP6466966B2 JP 6466966 B2 JP6466966 B2 JP 6466966B2 JP 2016561445 A JP2016561445 A JP 2016561445A JP 2016561445 A JP2016561445 A JP 2016561445A JP 6466966 B2 JP6466966 B2 JP 6466966B2
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
以上のように、さらなる微細化と高集積化の必要なDNAシーケンサでは、低コストの基板材料を採用しても、あるいは、光学的計測、電気的計測のどちらを採用しても、いずれにせよ、リソグラフィ、ドライエッチング微細加工などの半導体プロセスを用いて全ての高集積アレイ基板を製造している限り、製造コスト低減には限界がある。全ての基板の製造に半導体プロセスを適用しなくても量産できる製造技術の確立が望まれる。
実施例1として、樹脂基板を用いて、官能基スポットアレイ又はオリゴDNAスポットアレイを形成したスポットアレイ基板の製造例を説明する。
実用上好ましくはビーズ充填率が高く、少なくとも90%以上の有効なアレイスポットを形成することが望まれる。そこで、実施例2として、樹脂基板を用いて官能基スポットアレイやオリゴDNAスポットアレイなどの表面修飾スポットアレイを形成したチップを製造し、そのとき特に、ビーズ充填率を向上し、かつ分析中に脱落しない施策を施した例を示す。
次に、実施例3として、樹脂基板を用いて、官能基スポットアレイ又はオリゴDNAスポットアレイを形成したチップを製造し、そのとき特に、ビーズ充填率を上げかつ分析中に脱落しない別の施策として、ビーズの固定箇所での化学結合の効果並びにビーズ粒径分布の狭小化の効果を検討した。
実施例4として、スポットアレイ基板を組み込んだフローセルを用いた分析の例を示す。
実施例5として、スポットアレイ基板を組み込んだフローセルを用いた分析の他の例を示す。
207 遠心分離器
208 アミノ基表面修飾磁気ビーズ
210 磁石
304 凸部
305 金型
306 樹脂基板
405 遠心分離器
701 フローセル
702 反応試薬ユニット
709 光源
710 光学系
711 光検出器アレイ
712 温調機能
713 解析装置
714 アミノ基表面修飾ビーズ
715 表面にテンプレートDNAの複製を形成したビーズ
915 オリゴDNA表面修飾ビーズ
916 相補配列付きテンプレートDNA
Claims (1)
- 表面に凹凸パターンが形成され、前記凹凸パターン中に複数のビーズ着座位置が2次元アレイ状に設定されている樹脂基板を作製する工程と、
前記樹脂基板の前記ビーズ着座位置に表面修飾ビーズを装填する工程とを有し、
前記表面修飾ビーズは官能基表面修飾ビーズ又はオリゴDNA表面修飾ビーズであり、
前記樹脂基板の前記ビーズ着座位置に表面修飾ビーズを装填する工程は、前記樹脂基板を加熱して前記樹脂基板を熱膨張させた状態で前記ビーズ着座位置に前記表面修飾ビーズを装填する第1の工程と、その後、前記樹脂基板の温度を下げ、前記樹脂基板を収縮させて前記ビーズ着座位置に装填された表面修飾ビーズを脱落しにくくする第2の工程を有することを特徴とするスポットアレイ基板の製造方法。
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PCT/JP2015/078479 WO2016084489A1 (ja) | 2014-11-27 | 2015-10-07 | スポットアレイ基板、その製造方法、核酸ポリマー解析方法及び装置 |
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WO2019089581A1 (en) * | 2017-10-30 | 2019-05-09 | Corning Incorporated | Nucleic acid immobilization article and methods thereof |
US20210213414A1 (en) * | 2018-05-15 | 2021-07-15 | Biocopy Gmbh | Microarray transformer |
WO2020095405A1 (ja) * | 2018-11-08 | 2020-05-14 | 株式会社日立ハイテク | 生体分子分析用基板、生体分子分析用フローセル、及び生体分子解析方法 |
DE112019005939T5 (de) * | 2019-01-09 | 2021-09-09 | Hitachi High-Tech Corporation | Substrat für nukleinsäurenanalyse, durchflusszelle für nukleinsäurenanalyse und bildanalyseverfahren |
CN110607220B (zh) * | 2019-08-01 | 2023-03-14 | 广东工业大学 | 一种精确修饰生物分子的阵列式结构及其修饰方法 |
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WO2016084489A1 (ja) | 2016-06-02 |
JPWO2016084489A1 (ja) | 2017-08-24 |
US20170260573A1 (en) | 2017-09-14 |
JP2019022534A (ja) | 2019-02-14 |
DE112015004899B4 (de) | 2022-10-20 |
GB2546441B (en) | 2020-07-29 |
GB201706197D0 (en) | 2017-05-31 |
DE112015004899T5 (de) | 2017-08-10 |
JP6710259B2 (ja) | 2020-06-17 |
GB2546441A (en) | 2017-07-19 |
US11130985B2 (en) | 2021-09-28 |
CN107002071A (zh) | 2017-08-01 |
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