JP6466966B2 - スポットアレイ基板の製造方法 - Google Patents
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00421—Means for dispensing and evacuation of reagents using centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00457—Dispensing or evacuation of the solid phase support
- B01J2219/00459—Beads
- B01J2219/00466—Beads in a slurry
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
- B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B27/00—Layered products comprising a layer of synthetic resin
- B32B27/32—Layered products comprising a layer of synthetic resin comprising polyolefins
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G77/00—Macromolecular compounds obtained by reactions forming a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon in the main chain of the macromolecule
- C08G77/48—Macromolecular compounds obtained by reactions forming a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon in the main chain of the macromolecule in which at least two but not all the silicon atoms are connected by linkages other than oxygen atoms
- C08G77/50—Macromolecular compounds obtained by reactions forming a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon in the main chain of the macromolecule in which at least two but not all the silicon atoms are connected by linkages other than oxygen atoms by carbon linkages
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
Description
以上のように、さらなる微細化と高集積化の必要なDNAシーケンサでは、低コストの基板材料を採用しても、あるいは、光学的計測、電気的計測のどちらを採用しても、いずれにせよ、リソグラフィ、ドライエッチング微細加工などの半導体プロセスを用いて全ての高集積アレイ基板を製造している限り、製造コスト低減には限界がある。全ての基板の製造に半導体プロセスを適用しなくても量産できる製造技術の確立が望まれる。
実施例1として、樹脂基板を用いて、官能基スポットアレイ又はオリゴDNAスポットアレイを形成したスポットアレイ基板の製造例を説明する。
実用上好ましくはビーズ充填率が高く、少なくとも90%以上の有効なアレイスポットを形成することが望まれる。そこで、実施例2として、樹脂基板を用いて官能基スポットアレイやオリゴDNAスポットアレイなどの表面修飾スポットアレイを形成したチップを製造し、そのとき特に、ビーズ充填率を向上し、かつ分析中に脱落しない施策を施した例を示す。
次に、実施例3として、樹脂基板を用いて、官能基スポットアレイ又はオリゴDNAスポットアレイを形成したチップを製造し、そのとき特に、ビーズ充填率を上げかつ分析中に脱落しない別の施策として、ビーズの固定箇所での化学結合の効果並びにビーズ粒径分布の狭小化の効果を検討した。
実施例4として、スポットアレイ基板を組み込んだフローセルを用いた分析の例を示す。
実施例5として、スポットアレイ基板を組み込んだフローセルを用いた分析の他の例を示す。
207 遠心分離器
208 アミノ基表面修飾磁気ビーズ
210 磁石
304 凸部
305 金型
306 樹脂基板
405 遠心分離器
701 フローセル
702 反応試薬ユニット
709 光源
710 光学系
711 光検出器アレイ
712 温調機能
713 解析装置
714 アミノ基表面修飾ビーズ
715 表面にテンプレートDNAの複製を形成したビーズ
915 オリゴDNA表面修飾ビーズ
916 相補配列付きテンプレートDNA
Claims (1)
- 表面に凹凸パターンが形成され、前記凹凸パターン中に複数のビーズ着座位置が2次元アレイ状に設定されている樹脂基板を作製する工程と、
前記樹脂基板の前記ビーズ着座位置に表面修飾ビーズを装填する工程とを有し、
前記表面修飾ビーズは官能基表面修飾ビーズ又はオリゴDNA表面修飾ビーズであり、
前記樹脂基板の前記ビーズ着座位置に表面修飾ビーズを装填する工程は、前記樹脂基板を加熱して前記樹脂基板を熱膨張させた状態で前記ビーズ着座位置に前記表面修飾ビーズを装填する第1の工程と、その後、前記樹脂基板の温度を下げ、前記樹脂基板を収縮させて前記ビーズ着座位置に装填された表面修飾ビーズを脱落しにくくする第2の工程を有することを特徴とするスポットアレイ基板の製造方法。
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JP2014240434 | 2014-11-27 | ||
JP2014240434 | 2014-11-27 | ||
PCT/JP2015/078479 WO2016084489A1 (ja) | 2014-11-27 | 2015-10-07 | スポットアレイ基板、その製造方法、核酸ポリマー解析方法及び装置 |
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JP2018217491A Division JP6710259B2 (ja) | 2014-11-27 | 2018-11-20 | スポットアレイ基板、その製造方法、核酸ポリマー解析方法及び装置 |
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JPWO2016084489A1 JPWO2016084489A1 (ja) | 2017-08-24 |
JP6466966B2 true JP6466966B2 (ja) | 2019-02-06 |
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JP2016561445A Expired - Fee Related JP6466966B2 (ja) | 2014-11-27 | 2015-10-07 | スポットアレイ基板の製造方法 |
JP2018217491A Expired - Fee Related JP6710259B2 (ja) | 2014-11-27 | 2018-11-20 | スポットアレイ基板、その製造方法、核酸ポリマー解析方法及び装置 |
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JP2018217491A Expired - Fee Related JP6710259B2 (ja) | 2014-11-27 | 2018-11-20 | スポットアレイ基板、その製造方法、核酸ポリマー解析方法及び装置 |
Country Status (6)
Country | Link |
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US (1) | US11130985B2 (ja) |
JP (2) | JP6466966B2 (ja) |
CN (1) | CN107002071A (ja) |
DE (1) | DE112015004899B4 (ja) |
GB (1) | GB2546441B (ja) |
WO (1) | WO2016084489A1 (ja) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
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JP6729557B2 (ja) * | 2015-03-26 | 2020-07-22 | 東レ株式会社 | エアフィルター用濾材 |
WO2018100724A1 (ja) * | 2016-12-01 | 2018-06-07 | 株式会社日立ハイテクノロジーズ | スポットアレイ基板、核酸解析方法、及び核酸解析装置 |
US20210178353A1 (en) * | 2017-10-30 | 2021-06-17 | Corning Incorporated | Nucleic acid immobilization article and methods thereof |
WO2019219757A1 (de) * | 2018-05-15 | 2019-11-21 | Albert-Ludwig-Universität Freiburg | Mikroarray-transformer |
WO2020095405A1 (ja) * | 2018-11-08 | 2020-05-14 | 株式会社日立ハイテク | 生体分子分析用基板、生体分子分析用フローセル、及び生体分子解析方法 |
WO2020145124A1 (ja) * | 2019-01-09 | 2020-07-16 | 株式会社日立ハイテク | 核酸分析用基板、核酸分析用フローセル、及び画像解析方法 |
CN110607220B (zh) * | 2019-08-01 | 2023-03-14 | 广东工业大学 | 一种精确修饰生物分子的阵列式结构及其修饰方法 |
WO2021024416A1 (ja) * | 2019-08-07 | 2021-02-11 | 株式会社日立ハイテク | フローセルの調整方法 |
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US20020172980A1 (en) * | 2000-11-27 | 2002-11-21 | Phan Brigitte Chau | Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems |
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JP2004037338A (ja) * | 2002-07-05 | 2004-02-05 | Yokogawa Electric Corp | 磁気ビーズを用いて生体高分子を基板へ固定する方法およびその方法を用いた生体高分子測定装置 |
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JP5504587B2 (ja) * | 2008-05-27 | 2014-05-28 | 東レ株式会社 | 分析用チップ |
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JP5618556B2 (ja) * | 2010-01-28 | 2014-11-05 | 株式会社日立ハイテクノロジーズ | 核酸分析装置,核酸分析反応デバイス、および核酸分析用反応デバイス用基板 |
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JP5907990B2 (ja) | 2010-12-17 | 2016-05-11 | ライフ テクノロジーズ コーポレーション | 核酸増幅のための方法、組成物、システム、装置およびキット |
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WO2014176435A2 (en) * | 2013-04-25 | 2014-10-30 | Bergo Vladislav B | Microarray compositions and methods of their use |
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2015
- 2015-10-07 CN CN201580064597.9A patent/CN107002071A/zh active Pending
- 2015-10-07 JP JP2016561445A patent/JP6466966B2/ja not_active Expired - Fee Related
- 2015-10-07 DE DE112015004899.5T patent/DE112015004899B4/de active Active
- 2015-10-07 WO PCT/JP2015/078479 patent/WO2016084489A1/ja active Application Filing
- 2015-10-07 US US15/529,122 patent/US11130985B2/en active Active
- 2015-10-07 GB GB1706197.9A patent/GB2546441B/en active Active
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Also Published As
Publication number | Publication date |
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WO2016084489A1 (ja) | 2016-06-02 |
US20170260573A1 (en) | 2017-09-14 |
US11130985B2 (en) | 2021-09-28 |
JPWO2016084489A1 (ja) | 2017-08-24 |
GB2546441B (en) | 2020-07-29 |
JP2019022534A (ja) | 2019-02-14 |
CN107002071A (zh) | 2017-08-01 |
JP6710259B2 (ja) | 2020-06-17 |
DE112015004899T5 (de) | 2017-08-10 |
DE112015004899B4 (de) | 2022-10-20 |
GB2546441A (en) | 2017-07-19 |
GB201706197D0 (en) | 2017-05-31 |
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