JP6442411B2 - 新規な細胞株スクリーニング方法 - Google Patents
新規な細胞株スクリーニング方法 Download PDFInfo
- Publication number
- JP6442411B2 JP6442411B2 JP2015545470A JP2015545470A JP6442411B2 JP 6442411 B2 JP6442411 B2 JP 6442411B2 JP 2015545470 A JP2015545470 A JP 2015545470A JP 2015545470 A JP2015545470 A JP 2015545470A JP 6442411 B2 JP6442411 B2 JP 6442411B2
- Authority
- JP
- Japan
- Prior art keywords
- cell
- dna
- sequence
- cells
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims description 39
- 238000012216 screening Methods 0.000 title claims description 15
- 210000004027 cell Anatomy 0.000 claims description 171
- 230000014509 gene expression Effects 0.000 claims description 71
- 239000012528 membrane Substances 0.000 claims description 62
- 108020004414 DNA Proteins 0.000 claims description 60
- 108090000623 proteins and genes Proteins 0.000 claims description 58
- 108010091086 Recombinases Proteins 0.000 claims description 41
- 102000018120 Recombinases Human genes 0.000 claims description 41
- 102000004169 proteins and genes Human genes 0.000 claims description 40
- 239000001963 growth medium Substances 0.000 claims description 21
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims description 19
- 230000027455 binding Effects 0.000 claims description 14
- 108020003175 receptors Proteins 0.000 claims description 12
- 102000005962 receptors Human genes 0.000 claims description 12
- 108091092195 Intron Proteins 0.000 claims description 9
- 239000000427 antigen Substances 0.000 claims description 8
- 108091007433 antigens Proteins 0.000 claims description 8
- 102000036639 antigens Human genes 0.000 claims description 8
- 108700024394 Exon Proteins 0.000 claims description 5
- 230000004071 biological effect Effects 0.000 claims description 5
- 210000000349 chromosome Anatomy 0.000 claims description 3
- 108700008625 Reporter Genes Proteins 0.000 claims 3
- 102100034343 Integrase Human genes 0.000 claims 2
- 108010061833 Integrases Proteins 0.000 claims 2
- 101001010097 Shigella phage SfV Bactoprenol-linked glucose translocase Proteins 0.000 claims 2
- 239000006143 cell culture medium Substances 0.000 claims 1
- 239000013604 expression vector Substances 0.000 description 30
- 241000282414 Homo sapiens Species 0.000 description 28
- 239000013598 vector Substances 0.000 description 28
- 229960004641 rituximab Drugs 0.000 description 27
- 238000004519 manufacturing process Methods 0.000 description 20
- 238000013461 design Methods 0.000 description 17
- 238000002826 magnetic-activated cell sorting Methods 0.000 description 15
- 238000010790 dilution Methods 0.000 description 14
- 239000012895 dilution Substances 0.000 description 14
- 229940048921 humira Drugs 0.000 description 14
- 238000010586 diagram Methods 0.000 description 13
- 238000002965 ELISA Methods 0.000 description 11
- 108090000765 processed proteins & peptides Proteins 0.000 description 11
- 241000283707 Capra Species 0.000 description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 description 9
- 108010076504 Protein Sorting Signals Proteins 0.000 description 9
- 238000001890 transfection Methods 0.000 description 9
- 108060003951 Immunoglobulin Proteins 0.000 description 8
- 238000011965 cell line development Methods 0.000 description 8
- 238000000684 flow cytometry Methods 0.000 description 8
- 102000018358 immunoglobulin Human genes 0.000 description 8
- 238000003780 insertion Methods 0.000 description 8
- 230000037431 insertion Effects 0.000 description 8
- 230000003834 intracellular effect Effects 0.000 description 8
- 210000004962 mammalian cell Anatomy 0.000 description 8
- 230000007246 mechanism Effects 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
- 238000012270 DNA recombination Methods 0.000 description 7
- 108010052285 Membrane Proteins Proteins 0.000 description 7
- 102000018697 Membrane Proteins Human genes 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 230000001225 therapeutic effect Effects 0.000 description 7
- 239000012096 transfection reagent Substances 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 6
- 229920001184 polypeptide Polymers 0.000 description 6
- 102000004196 processed proteins & peptides Human genes 0.000 description 6
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 6
- 108020004684 Internal Ribosome Entry Sites Proteins 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 5
- 230000004481 post-translational protein modification Effects 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 4
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 4
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 230000004186 co-expression Effects 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 230000013595 glycosylation Effects 0.000 description 4
- 238000006206 glycosylation reaction Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 101710200251 Recombinase cre Proteins 0.000 description 3
- 108010022394 Threonine synthase Proteins 0.000 description 3
- 101100343202 Vicia faba LB29 gene Proteins 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 238000004220 aggregation Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 102000004419 dihydrofolate reductase Human genes 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 230000016784 immunoglobulin production Effects 0.000 description 3
- 229940072221 immunoglobulins Drugs 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 229950010131 puromycin Drugs 0.000 description 3
- 229960000160 recombinant therapeutic protein Drugs 0.000 description 3
- 238000003757 reverse transcription PCR Methods 0.000 description 3
- 230000003248 secreting effect Effects 0.000 description 3
- 102000019260 B-Cell Antigen Receptors Human genes 0.000 description 2
- 108010012919 B-Cell Antigen Receptors Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 108010051219 Cre recombinase Proteins 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 101000692455 Homo sapiens Platelet-derived growth factor receptor beta Proteins 0.000 description 2
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 2
- 102100026547 Platelet-derived growth factor receptor beta Human genes 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 210000000628 antibody-producing cell Anatomy 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000013537 high throughput screening Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- SXTAYKAGBXMACB-UHFFFAOYSA-N methionine S-imide-S-oxide Natural products CS(=N)(=O)CCC(N)C(O)=O SXTAYKAGBXMACB-UHFFFAOYSA-N 0.000 description 2
- 229960000485 methotrexate Drugs 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 230000030648 nucleus localization Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 150000003905 phosphatidylinositols Chemical group 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 230000010474 transient expression Effects 0.000 description 2
- 238000003146 transient transfection Methods 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108091006020 Fc-tagged proteins Proteins 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 101100386242 Homo sapiens CD55 gene Proteins 0.000 description 1
- 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 description 1
- 108700005091 Immunoglobulin Genes Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 101710193739 Protein RecA Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 241000710960 Sindbis virus Species 0.000 description 1
- 108010052160 Site-specific recombinase Proteins 0.000 description 1
- -1 adhesion molecules Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940124691 antibody therapeutics Drugs 0.000 description 1
- 230000009831 antigen interaction Effects 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003114 blood coagulation factor Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000009615 deamination Effects 0.000 description 1
- 238000006481 deamination reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000011162 downstream development Methods 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 102000057041 human TNF Human genes 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N inositol Chemical group OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000009126 molecular therapy Methods 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 229910052754 neon Inorganic materials 0.000 description 1
- GKAOGPIIYCISHV-UHFFFAOYSA-N neon atom Chemical compound [Ne] GKAOGPIIYCISHV-UHFFFAOYSA-N 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 238000002818 protein evolution Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 238000003153 stable transfection Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Food Science & Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261732156P | 2012-11-30 | 2012-11-30 | |
| US61/732,156 | 2012-11-30 | ||
| PCT/US2013/072412 WO2014085711A1 (en) | 2012-11-30 | 2013-11-27 | A novel cell line screening method |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018167792A Division JP6785272B2 (ja) | 2012-11-30 | 2018-09-07 | 新規な細胞株スクリーニング方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016506239A JP2016506239A (ja) | 2016-03-03 |
| JP2016506239A5 JP2016506239A5 (enExample) | 2016-12-28 |
| JP6442411B2 true JP6442411B2 (ja) | 2018-12-26 |
Family
ID=49880972
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015545470A Expired - Fee Related JP6442411B2 (ja) | 2012-11-30 | 2013-11-27 | 新規な細胞株スクリーニング方法 |
| JP2018167792A Expired - Fee Related JP6785272B2 (ja) | 2012-11-30 | 2018-09-07 | 新規な細胞株スクリーニング方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018167792A Expired - Fee Related JP6785272B2 (ja) | 2012-11-30 | 2018-09-07 | 新規な細胞株スクリーニング方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9910038B2 (enExample) |
| EP (2) | EP3382026B1 (enExample) |
| JP (2) | JP6442411B2 (enExample) |
| CN (2) | CN104870646B (enExample) |
| WO (1) | WO2014085711A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6442411B2 (ja) * | 2012-11-30 | 2018-12-26 | ラリクス・バイオサイエンス・リミテッド・ライアビリティ・カンパニーＬａｒｉｘ Ｂｉｏｓｃｉｅｎｃｅ， Ｌｌｃ | 新規な細胞株スクリーニング方法 |
| WO2016132122A1 (en) * | 2015-02-17 | 2016-08-25 | University Of Edinburgh | Assay construct |
| KR20210030414A (ko) * | 2018-07-09 | 2021-03-17 | 제노비에 에이비 | 조작된 수용체 세포로의 핵산 서열의 재조합효소 매개 카세트 교환 통합 동안 세포의 추적 및 조작을 위한 세포 표면 태그 교환(cste) 시스템 |
| US20210356377A1 (en) * | 2018-09-07 | 2021-11-18 | Medimmune, Llc | Methods Of Cell Selection |
| KR20220025806A (ko) * | 2019-06-26 | 2022-03-03 | 제넨테크, 인크. | 핵산의 무작위 구성 표적화 통합 |
| WO2025176217A1 (en) * | 2024-02-22 | 2025-08-28 | The University Of Hong Kong | Conditional splicing system for tight control of viral overlapping genes |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001094571A1 (fr) * | 2000-06-08 | 2001-12-13 | Mitsubishi Pharma Corporation | Vecteur d'expression permettant le criblage de cellules a haute expression de proteine de recombinaison, transformant a haute expression de proteine de recombinaison, et leur utilisation |
| US6406863B1 (en) | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| US20030059763A1 (en) * | 2001-01-26 | 2003-03-27 | Andrew Saxon | Immunoglobulin class switch recombination |
| GB0118337D0 (en) | 2001-07-27 | 2001-09-19 | Lonza Biologics Plc | Method for selecting antibody expressing cells |
| AU2003208876A1 (en) * | 2002-02-21 | 2003-09-09 | Vision 7 Gmbh | Methods for conducting site-specific dna recombination |
| US7119249B2 (en) * | 2002-07-12 | 2006-10-10 | Emory University | Methods and transgenic mouse model for identifying and modulating factors involved in the production of reactive oxygen intermediates |
| US20040115814A1 (en) * | 2002-09-30 | 2004-06-17 | Protein Design Labs, Inc. | Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes |
| ES2329807T3 (es) * | 2004-01-30 | 2009-12-01 | Maxygen Holdings Ltd. | Translectura regulada de codones de terminacion. |
| KR20080073293A (ko) | 2005-10-14 | 2008-08-08 | 메디뮨 엘엘씨 | 항체 라이브러리의 세포 디스플레이 |
| WO2008070367A2 (en) | 2006-11-01 | 2008-06-12 | Facet Biotech Corporation | Mammalian cell-based immunoglobulin display libraries |
| EP2150617B1 (en) | 2007-06-04 | 2014-10-22 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| WO2009059235A2 (en) * | 2007-11-01 | 2009-05-07 | Facet Biotech Corporation | Immunoglobulin display vectors |
| RU2528858C2 (ru) * | 2008-08-28 | 2014-09-20 | Новартис Аг | Дисплей на поверхности клеток полипептидных изоформ на основе прочитывания терминирующего кодона |
| CN101671653B (zh) * | 2009-09-22 | 2012-05-23 | 浙江大学 | 一种hiv侵染细胞的筛选系统及其应用 |
| US20130071881A1 (en) * | 2009-11-19 | 2013-03-21 | Immuno Tec Laboratory Co. Ltd | Methods for producing antibody-producing cells that produce desired polypeptides |
| JP2011182645A (ja) * | 2010-03-04 | 2011-09-22 | Kyushu Univ | 動物細胞ディスプレイ法およびそれを利用した完全ヒトモノクローナル抗体の体外成熟法 |
| JP6442411B2 (ja) * | 2012-11-30 | 2018-12-26 | ラリクス・バイオサイエンス・リミテッド・ライアビリティ・カンパニーＬａｒｉｘ Ｂｉｏｓｃｉｅｎｃｅ， Ｌｌｃ | 新規な細胞株スクリーニング方法 |
-
2013
- 2013-11-27 JP JP2015545470A patent/JP6442411B2/ja not_active Expired - Fee Related
- 2013-11-27 CN CN201380062665.9A patent/CN104870646B/zh active Active
- 2013-11-27 WO PCT/US2013/072412 patent/WO2014085711A1/en not_active Ceased
- 2013-11-27 EP EP18000323.8A patent/EP3382026B1/en active Active
- 2013-11-27 EP EP13812267.6A patent/EP2925867B1/en active Active
- 2013-11-27 CN CN201910335307.XA patent/CN110257412B/zh not_active Expired - Fee Related
- 2013-11-27 US US14/092,786 patent/US9910038B2/en active Active
-
2018
- 2018-02-19 US US15/899,051 patent/US10816548B2/en active Active
- 2018-09-07 JP JP2018167792A patent/JP6785272B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP3382026B1 (en) | 2021-01-06 |
| CN104870646A (zh) | 2015-08-26 |
| CN110257412A (zh) | 2019-09-20 |
| US20140155285A1 (en) | 2014-06-05 |
| US10816548B2 (en) | 2020-10-27 |
| CN104870646B (zh) | 2019-05-28 |
| JP6785272B2 (ja) | 2020-11-18 |
| EP3382026A1 (en) | 2018-10-03 |
| EP2925867A1 (en) | 2015-10-07 |
| WO2014085711A1 (en) | 2014-06-05 |
| US9910038B2 (en) | 2018-03-06 |
| JP2016506239A (ja) | 2016-03-03 |
| CN110257412B (zh) | 2023-12-15 |
| JP2018201520A (ja) | 2018-12-27 |
| US20180313834A1 (en) | 2018-11-01 |
| EP2925867B1 (en) | 2018-06-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6785272B2 (ja) | 新規な細胞株スクリーニング方法 | |
| US7112439B2 (en) | Dual expression vector system for antibody expression in bacterial and mammalian cells | |
| EP3047022B1 (en) | Expression vector production and high-throughput cell screening | |
| US10174309B2 (en) | Transposition-mediated identification of specific binding or functional proteins | |
| EP2401295B1 (en) | Method for producing antibodies | |
| AU2017213633B2 (en) | Enhanced production of immunoglobulins | |
| JP2004520037A (ja) | 分泌タンパク質を発現する細胞の単離 | |
| JP6683598B2 (ja) | 安定なトランスフェクトされた細胞を生成するための方法および組成物 | |
| JP2024009895A (ja) | 真核細胞ディスプレイ系におけるポリペプチド薬物の開発性のための選択 | |
| US20200072820A1 (en) | Method of Selecting for Antibodies | |
| KR20230150779A (ko) | 중쇄-단독 항체 | |
| US20190031752A1 (en) | Method for Producing Antibodies | |
| US8969539B2 (en) | Expression vectors | |
| Hashimoto et al. | B Cell-based seamless engineering of antibody Fc domains | |
| WO2013191199A1 (ja) | タンパク質の迅速改良法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20161108 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20161108 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20170913 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170919 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20171205 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20180508 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180907 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20180907 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20181010 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20181030 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20181126 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6442411 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |