JP2016506239A5 - - Google Patents
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- JP2016506239A5 JP2016506239A5 JP2015545470A JP2015545470A JP2016506239A5 JP 2016506239 A5 JP2016506239 A5 JP 2016506239A5 JP 2015545470 A JP2015545470 A JP 2015545470A JP 2015545470 A JP2015545470 A JP 2015545470A JP 2016506239 A5 JP2016506239 A5 JP 2016506239A5
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- JP
- Japan
- Prior art keywords
- dna
- interest
- cell
- gene
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 239000012528 membrane Substances 0.000 claims description 13
- 108020004705 Codon Proteins 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 claims 18
- 108090000623 proteins and genes Proteins 0.000 claims 18
- 108010091086 Recombinases Proteins 0.000 claims 16
- 102000018120 Recombinases Human genes 0.000 claims 16
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 102000004169 proteins and genes Human genes 0.000 claims 7
- 238000000034 method Methods 0.000 claims 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims 5
- 102000005962 receptors Human genes 0.000 claims 4
- 108020003175 receptors Proteins 0.000 claims 4
- 229920001184 polypeptide Polymers 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- 238000011144 upstream manufacturing Methods 0.000 claims 3
- 108010051219 Cre recombinase Proteins 0.000 claims 2
- 108010046276 FLP recombinase Proteins 0.000 claims 2
- 102100034343 Integrase Human genes 0.000 claims 2
- 108010061833 Integrases Proteins 0.000 claims 2
- 108010052160 Site-specific recombinase Proteins 0.000 claims 2
- 230000004927 fusion Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 238000012216 screening Methods 0.000 claims 2
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 230000004071 biological effect Effects 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 229960004641 rituximab Drugs 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000012096 transfection reagent Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261732156P | 2012-11-30 | 2012-11-30 | |
| US61/732,156 | 2012-11-30 | ||
| PCT/US2013/072412 WO2014085711A1 (en) | 2012-11-30 | 2013-11-27 | A novel cell line screening method |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018167792A Division JP6785272B2 (ja) | 2012-11-30 | 2018-09-07 | 新規な細胞株スクリーニング方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016506239A JP2016506239A (ja) | 2016-03-03 |
| JP2016506239A5 true JP2016506239A5 (enExample) | 2016-12-28 |
| JP6442411B2 JP6442411B2 (ja) | 2018-12-26 |
Family
ID=49880972
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015545470A Expired - Fee Related JP6442411B2 (ja) | 2012-11-30 | 2013-11-27 | 新規な細胞株スクリーニング方法 |
| JP2018167792A Expired - Fee Related JP6785272B2 (ja) | 2012-11-30 | 2018-09-07 | 新規な細胞株スクリーニング方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018167792A Expired - Fee Related JP6785272B2 (ja) | 2012-11-30 | 2018-09-07 | 新規な細胞株スクリーニング方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9910038B2 (enExample) |
| EP (2) | EP2925867B1 (enExample) |
| JP (2) | JP6442411B2 (enExample) |
| CN (2) | CN104870646B (enExample) |
| WO (1) | WO2014085711A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104870646B (zh) * | 2012-11-30 | 2019-05-28 | 落叶松生物科学公司 | 一种新颖的细胞系筛选方法 |
| WO2016132122A1 (en) * | 2015-02-17 | 2016-08-25 | University Of Edinburgh | Assay construct |
| US20210371875A1 (en) * | 2018-07-09 | 2021-12-02 | Genovie Ab | A cell surface tag exchange (cste) system for tracing and manipulation of cells during recombinase mediated cassette exchange integration of nucleic acid sequences to engineered receiver cells |
| EP3847271A4 (en) * | 2018-09-07 | 2022-10-12 | MedImmune, LLC | Methods of cell selection |
| CN114080454A (zh) * | 2019-06-26 | 2022-02-22 | 豪夫迈·罗氏有限公司 | 核酸的随机化构型靶向整合 |
| WO2025176217A1 (en) * | 2024-02-22 | 2025-08-28 | The University Of Hong Kong | Conditional splicing system for tight control of viral overlapping genes |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1293564A4 (en) * | 2000-06-08 | 2005-08-17 | Mitsubishi Pharma Corp | EXPRESSION VECTOR FOR SCREENING CELLS HIGH EXPRESSION OF RECOMBINANT PROTEINS, TRANSFORMING HIGH EXPRESSION OF A RECOMBINANT PROTEIN AND USE THEREOF |
| US6406863B1 (en) | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| US20030059763A1 (en) * | 2001-01-26 | 2003-03-27 | Andrew Saxon | Immunoglobulin class switch recombination |
| GB0118337D0 (en) | 2001-07-27 | 2001-09-19 | Lonza Biologics Plc | Method for selecting antibody expressing cells |
| AU2003208876A1 (en) | 2002-02-21 | 2003-09-09 | Vision 7 Gmbh | Methods for conducting site-specific dna recombination |
| US7119249B2 (en) * | 2002-07-12 | 2006-10-10 | Emory University | Methods and transgenic mouse model for identifying and modulating factors involved in the production of reactive oxygen intermediates |
| US20040115814A1 (en) * | 2002-09-30 | 2004-06-17 | Protein Design Labs, Inc. | Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes |
| DK1716233T3 (da) * | 2004-01-30 | 2009-10-26 | Maxygen Holdings Ltd | Reguleret stopkodongennemlæsning |
| CA2625619A1 (en) | 2005-10-14 | 2007-04-26 | Medimmune, Inc. | Cell display of antibody libraries |
| US7732195B2 (en) | 2006-11-01 | 2010-06-08 | Facet Biotech Corporation | Tethered vectors for cell surface immunoglobulin display |
| WO2008151219A1 (en) | 2007-06-04 | 2008-12-11 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| WO2009059235A2 (en) * | 2007-11-01 | 2009-05-07 | Facet Biotech Corporation | Immunoglobulin display vectors |
| MX2011002273A (es) * | 2008-08-28 | 2011-03-30 | Novartis Ag | Despliegue de isoformas de polipeptido en la superficie celular por ultralectura del codon de paro. |
| CN101671653B (zh) * | 2009-09-22 | 2012-05-23 | 浙江大学 | 一种hiv侵染细胞的筛选系统及其应用 |
| EP2501810A4 (en) * | 2009-11-19 | 2014-05-28 | Immuno Tec Lab Co Ltd | METHOD FOR PRODUCING ANTIBODY-PROPERING CELLS FOR THE PREPARATION OF DESIRED POLYPEPTIDES |
| JP2011182645A (ja) * | 2010-03-04 | 2011-09-22 | Kyushu Univ | 動物細胞ディスプレイ法およびそれを利用した完全ヒトモノクローナル抗体の体外成熟法 |
| CN104870646B (zh) * | 2012-11-30 | 2019-05-28 | 落叶松生物科学公司 | 一种新颖的细胞系筛选方法 |
-
2013
- 2013-11-27 CN CN201380062665.9A patent/CN104870646B/zh active Active
- 2013-11-27 WO PCT/US2013/072412 patent/WO2014085711A1/en not_active Ceased
- 2013-11-27 US US14/092,786 patent/US9910038B2/en active Active
- 2013-11-27 CN CN201910335307.XA patent/CN110257412B/zh not_active Expired - Fee Related
- 2013-11-27 EP EP13812267.6A patent/EP2925867B1/en active Active
- 2013-11-27 JP JP2015545470A patent/JP6442411B2/ja not_active Expired - Fee Related
- 2013-11-27 EP EP18000323.8A patent/EP3382026B1/en active Active
-
2018
- 2018-02-19 US US15/899,051 patent/US10816548B2/en active Active
- 2018-09-07 JP JP2018167792A patent/JP6785272B2/ja not_active Expired - Fee Related
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