JP6430253B2 - 相互作用を検出および測定するための核酸ベースのリンカー - Google Patents

相互作用を検出および測定するための核酸ベースのリンカー Download PDF

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JP6430253B2
JP6430253B2 JP2014540177A JP2014540177A JP6430253B2 JP 6430253 B2 JP6430253 B2 JP 6430253B2 JP 2014540177 A JP2014540177 A JP 2014540177A JP 2014540177 A JP2014540177 A JP 2014540177A JP 6430253 B2 JP6430253 B2 JP 6430253B2
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nucleic acid
binding
binding partner
oligonucleotide
oligonucleotides
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JP2014532430A (ja
JP2014532430A5 (OSRAM
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フィリップ ウォン，ウェズリー
フィリップ ウォン，ウェズリー
アンダース ハルヴォルセン，ケネス
アンダース ハルヴォルセン，ケネス
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Boston Childrens Hospital
Harvard University
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Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2014540177A 2011-11-05 2012-11-05 相互作用を検出および測定するための核酸ベースのリンカー Active JP6430253B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161556194P 2011-11-05 2011-11-05
US61/556,194 2011-11-05
US201261666655P 2012-06-29 2012-06-29
US61/666,655 2012-06-29
PCT/US2012/063538 WO2013067489A1 (en) 2011-11-05 2012-11-05 Nucleic acid-based linkers for detecting and measuring interactions

Publications (3)

Publication Number Publication Date
JP2014532430A JP2014532430A (ja) 2014-12-08
JP2014532430A5 JP2014532430A5 (OSRAM) 2015-12-24
JP6430253B2 true JP6430253B2 (ja) 2018-11-28

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JP2014540177A Active JP6430253B2 (ja) 2011-11-05 2012-11-05 相互作用を検出および測定するための核酸ベースのリンカー

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US (2) US9914958B2 (OSRAM)
JP (1) JP6430253B2 (OSRAM)
WO (1) WO2013067489A1 (OSRAM)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6430253B2 (ja) * 2011-11-05 2018-11-28 プレジデント アンド フェローズ オブ ハーバード カレッジ 相互作用を検出および測定するための核酸ベースのリンカー
CN105555794A (zh) 2013-07-10 2016-05-04 哈佛学院院长及董事 涉及核酸-蛋白质复合物的组合物和方法
JP2017514467A (ja) * 2014-04-23 2017-06-08 チルドレンズ メディカル センター コーポレーション 核酸キャリパーを用いた高スループット構造決定
WO2016089588A1 (en) * 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
WO2016196824A1 (en) * 2015-06-02 2016-12-08 Children's Medical Center Corporation Nucleic acid complexes for screening barcoded compounds
US12077807B2 (en) 2015-06-27 2024-09-03 The Research Foundation For The State University Of New York Compositions and methods for analyte detection using nanoswitches
WO2017055463A1 (en) * 2015-10-01 2017-04-06 Dynamic Biosensors Gmbh Method for detecting molecular interactions using an immobilized nucleic acid
US11713483B2 (en) 2016-02-09 2023-08-01 Children's Medical Center Corporation Method for detection of analytes via polymer complexes
WO2017147398A1 (en) 2016-02-25 2017-08-31 Children's Medical Center Corporation Spinning apparatus for measurement of characteristics relating to molecules
EP3426598B1 (en) 2016-03-11 2021-07-28 Children's Medical Center Corporation Nucleic acid nanoswitch catenanes
WO2017165647A1 (en) * 2016-03-23 2017-09-28 Children's Medical Center Corporation Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods
WO2017165585A1 (en) 2016-03-23 2017-09-28 Children's Medical Center Corporation Systems and apparatus for detecting compounds in human biological samples
WO2017189794A1 (en) * 2016-04-27 2017-11-02 President And Fellows Of Harvard College Method of secure communication via nucleotide polymers
WO2018023088A1 (en) 2016-07-29 2018-02-01 Confer Health, Inc. Gel electrophoresis diagnostic kit and methods of using the same
US11254972B2 (en) 2016-08-02 2022-02-22 President And Fellows Of Harvard College Crisscross cooperative self-assembly
WO2018106721A1 (en) * 2016-12-05 2018-06-14 Children's Medical Center Corporation Nucleic acid nanoswitch construction methods
WO2018119437A2 (en) 2016-12-23 2018-06-28 Confer Health, Inc. Electrophoresis diagnostic methods and kits
WO2019100080A1 (en) 2017-11-20 2019-05-23 Children's Medical Center Corporation Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
FR3075820B1 (fr) 2017-12-21 2022-12-30 Paris Sciences Lettres Quartier Latin Molecule d'adn double-brin pour la detection et la caracterisation des interactions moleculaires
US11561221B2 (en) * 2018-05-02 2023-01-24 Trustees Of Boston University Dynamic tracking of captured targets for enhanced digital biosensing
WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
AU2020218190A1 (en) * 2019-02-04 2021-08-26 Akoya Biosciences, Inc. Analyte detection by selective labeling of biological samples
CN111549098B (zh) * 2020-06-16 2024-10-29 南开大学 测量人端粒精准长度的单分子力学方法
CN111721504B (zh) * 2020-06-17 2022-02-18 中国科学院物理研究所 在小力下测算磁镊高度转换系数的方法及应用
EP4504968A1 (en) 2022-04-01 2025-02-12 Depixus SAS Methods of screening compounds
WO2025041065A1 (en) * 2023-08-21 2025-02-27 Depixus SAS Device for determining molecular interactions

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9114525D0 (en) 1991-07-05 1991-08-21 Delnatte Sabine Homogeneous nucleic acids probe based tests and substances
AU7323194A (en) 1993-07-02 1995-01-24 Lynx Therapeutics, Inc. Synthesis of branched nucleic acids
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
EP0938588B1 (en) * 1996-07-12 2003-02-12 TM Technologies Inc. Signal amplification method
WO1999026724A2 (en) 1997-11-25 1999-06-03 Mosaic Technologies Devices and methods for detecting target molecules in biological samples
US20020177144A1 (en) 1997-12-30 2002-11-28 Jose Remacle Detection and/or quantification method of a target molecule by a binding with a capture molecule fixed on the surface of a disc
US6143504A (en) * 1998-10-27 2000-11-07 Arch Development Corporation Methods and compositions for the diagnosis of fragile X syndrome
GB9827912D0 (en) * 1998-12-19 1999-02-10 Univ Manchester Detecting nucleic acids
JP3080951B1 (ja) * 1999-03-04 2000-08-28 株式会社分子バイオホトニクス研究所 ウイルスを検出する方法
JP4436672B2 (ja) 2001-07-11 2010-03-24 アプライド バイオシステムズ， エルエルシー 電気泳動分析のための内部較正標準
US20030143549A1 (en) * 2002-01-29 2003-07-31 Eastman Kodak Company Method for DNA sequencing and gene identification
US7294513B2 (en) 2002-07-24 2007-11-13 Wyatt Technology Corporation Method and apparatus for characterizing solutions of small particles
JP4657919B2 (ja) 2002-08-19 2011-03-23 プレジデント アンド フェローズ オブ ハーバード カレッジ 進化する新しい分子機能
AU2002952797A0 (en) 2002-11-20 2002-12-05 Bio-Molecular Holdings Pty Limited Centrifugal device and method using same
EP2365095A1 (en) * 2003-02-26 2011-09-14 Callida Genomics, Inc. Random array DNA analysis by hybridization
JP4318721B2 (ja) 2004-10-15 2009-08-26 独立行政法人科学技術振興機構 ｍＲＮＡ−ピューロマイシン−タンパク質連結体作製用リンカー
US20070026423A1 (en) * 2005-03-16 2007-02-01 Thomas Koehler Method and test kit for the detection of target nucleic acids
US20070154899A1 (en) * 2005-05-26 2007-07-05 Coull James M Biodetection by nucleic acid-templated chemistry
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
US20100035247A1 (en) 2005-11-04 2010-02-11 U.S. Genomics, Inc. Heterogeneous Assay of Analytes in Solution Using Polymers
CA2640385C (en) * 2005-12-23 2014-07-15 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
EP1987166A4 (en) * 2006-02-03 2010-06-02 Univ Columbia BINARY DEOXYRIBOZYM PROBES FOR NUCLEIC ACID ANALYSIS
US8685894B2 (en) * 2006-05-25 2014-04-01 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University Modified nucleic acid nanoarrays and uses therefor
US20090286694A1 (en) 2006-08-21 2009-11-19 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
JP2008259453A (ja) * 2007-04-12 2008-10-30 Toyobo Co Ltd 核酸の検出方法
US8043810B2 (en) 2007-05-02 2011-10-25 Eagle Eye Research, Inc. Analyte detection using autocatalytic chain reactions
US8361299B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
EP2361098B1 (en) 2008-10-31 2019-08-28 The General Hospital Corporation Compositions and methods for delivering a substance to a biological target
US9354189B2 (en) 2008-12-02 2016-05-31 President And Fellows Of Harvard College Apparatus for measurement of spinning forces relating to molecules
US8491454B2 (en) 2008-12-02 2013-07-23 President And Fellows Of Harvard College Spinning force apparatus
JP2012532607A (ja) 2009-07-07 2012-12-20 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 一対の結合パートナーを同定する方法
EP3236264A3 (en) * 2009-10-13 2017-11-08 Nanostring Technologies, Inc Protein detection via nanoreporters
US9138418B2 (en) 2009-12-09 2015-09-22 William Marsh Rice University Therapeutic compositions and methods for delivery of active agents cleavably linked to nanoparticles
US20130196341A1 (en) 2010-07-06 2013-08-01 T2 Biosystems ,Inc. Methods and compositions for detection of analytes
JP6430253B2 (ja) * 2011-11-05 2018-11-28 プレジデント アンド フェローズ オブ ハーバード カレッジ 相互作用を検出および測定するための核酸ベースのリンカー
US9182334B2 (en) 2012-02-24 2015-11-10 Agilent Technologies, Inc. Method of determining thermodynamic and kinetic parameters from measured off rates
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
WO2013177046A1 (en) 2012-05-21 2013-11-28 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
WO2014011800A1 (en) 2012-07-10 2014-01-16 Pivot Bio, Inc. Methods for multipart, modular and scarless assembly of dna molecules
CN105555794A (zh) 2013-07-10 2016-05-04 哈佛学院院长及董事 涉及核酸-蛋白质复合物的组合物和方法
US9333463B2 (en) 2013-07-26 2016-05-10 General Electric Company Devices and systems for elution of biomolecules
US9322051B2 (en) 2013-10-07 2016-04-26 General Electric Company Probing of biological samples
US20150361422A1 (en) 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
WO2016089588A1 (en) 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
US12077807B2 (en) 2015-06-27 2024-09-03 The Research Foundation For The State University Of New York Compositions and methods for analyte detection using nanoswitches

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Publication number Publication date
WO2013067489A1 (en) 2013-05-10
JP2014532430A (ja) 2014-12-08
US9914958B2 (en) 2018-03-13
US20140255939A1 (en) 2014-09-11
US20180291434A1 (en) 2018-10-11
WO2013067489A9 (en) 2013-09-12

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